scholarly journals Effect of duration and dilution on antimicrobial efficacy of octenidine hydrochloride as an intracanal medicament with chitosan carrier against Enterococcus faecalis – A modified direct contact test

2020 ◽  
Vol 23 (5) ◽  
pp. 463
Author(s):  
VinayaSusan Varghese ◽  
Nirmal Kurian
Keyword(s):  
Enterococcus Faecalis ◽  
Direct Contact ◽  
Antimicrobial Efficacy ◽  
Contact Test ◽  
Direct Contact Test

scholarly journals An Assessment of Antibacterial Activity of Four Endodontic Sealers on Enterococcus faecalis by a Direct Contact Test: An In Vitro Study

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Lavanya Anumula ◽  
Swaroop Kumar ◽  
Venkata Suneel Kumar ◽  
Chandra Sekhar ◽  
Murali Krishna ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Direct Contact ◽  
Glass Ionomer ◽  
Polydimethyl Siloxane ◽  
Contact Test ◽  
Urethane Dimethacrylate ◽  
Endodontic Sealers ◽  
Direct Contact Test

Aim. To evaluate the antibacterial activity of four endodontic sealers on Enterococcus faecalis by a direct contact test. Material and Methods. Enterococcus faecalis was used as a test organism. Direct contact test which is based on measuring the effect of close contact between test bacteria and tested material on the kinetics of bacterial growth was performed to overcome the disadvantages of agar diffusion test. The sealers tested were zinc oxide eugenol-based sealer, glass-ionomer-based sealer, polydimethyl-siloxane-based sealer, and urethane dimethacrylate resin-based sealer. Data was collected by recording the optical density with the help of a spectrophotometer. Results. The sealers exhibited different inhibitory effects. The results obtained were subjected to statistical analysis by Kruskal Wallis analysis of variance and Dunn's multiple comparison test. Group comparison showed very highly significant difference between the groups. Conclusion. Zinc oxide eugenol-based sealer was the most effective and urethane dimethacrylate resin-based sealer was the least effective against Enterococcus faecalis, whereas glass-ionomer-based and polydimethyl-siloxane-based sealers were effective only for a short period. Inhibition of the bacterial growth is related to the direct contact of the microorganism with the sealer.

scholarly journals An assessment of antibacterial activity of three pulp capping materials on Enterococcus faecalis by a direct contact test: An in vitro study

2015 ◽  
Vol 09 (02) ◽  
pp. 240-245 ◽  
Author(s):  
Mine Koruyucu ◽  
Nursen Topcuoglu ◽  
E. Bahar Tuna ◽  
Sevda Ozel ◽  
Koray Gencay ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Direct Contact ◽  
In Vitro Study ◽  
Bacterial Density ◽  
Control Group ◽  
Pulp Capping ◽  
Contact Test ◽  
Direct Contact Test

ABSTRACT Objective: The aim of this in vitro study was to evaluate antimicrobial activities of three different pulp capping materials; Biodentine, mineral trioxide aggregate (MTA) Angelus, and Dycal against Enterococcus faecalis and their durability with time. Materials and Methods: Direct contact test was used for the assessment. Three sets of sealers were mixed and placed on microtiter plate wells: One set was used within 20 min of recommended setting time while others were used after 24-h and 1-week. E. faecalis suspension was placed directly on the materials for 1 h and then transferred to another plate with fresh media. Nine wells of bacteria without the tested cements served as the positive control. One well of the tested cements without bacteria served as the negative control. Bacterial growth was evaluated by a temperature-controlled microplate spectrophotometer for 1-h intervals among 24 h. Data were analyzed using Kruskal–Wallis test. Results: All tested materials showed less bacterial density than the control group. MTA, Biodentine, and Dycal showed significantly higher bacterial density than the control group in freshly mixed samples (P < 0.05). And MTA showed significantly higher antibacterial activity than Dycal (P < 0.05). In 24 h, materials did not show any differences (P > 0.05). MTA and Biodentine samples showed significant differences than Dycal; MTA also showed higher antibacterial activity than control in 1-week samples (P < 0.05). Conclusion: While freshly mixed MTA showed the best antibacterial activity over time, Biodentine had shown similar antibacterial activity to MTA.

scholarly journals Evaluation of Antimicrobial Efficacy of Pudina (Menthaarvenis), Tulsi (Ocimumtenuiflorum) and Curry Leaves (Murrayakoenigii) on Streptococcus mutans by Modified Direct Contact Test- An In vitro Study

2020 ◽  
Author(s):  
Hima Patel ◽  
Adish Anand Saraf ◽  
TM Mangala ◽  
Rushikesh Mahaparale ◽  
Sneha Mali ◽  
...  
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Direct Contact ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antimicrobial Efficacy ◽  
Contact Test ◽  
Curry Leaves ◽  
Direct Contact Test

Introduction: Dental caries is a pandemic disease caused by Streptococcus mutans. Numerous preventive strategies have been developed to prevent dental caries with modern medicines. Recently, there has been a shift from use of modern medicines to herbal ayurvedic preparations which are easily available and cause least possible side effects but have not been tested against S. mutans. Aim: To evaluate antimicrobial value of Pudina, Tulsi and Curry leaves against S. mutans by modified direct contact test. Materials and Methods: This in vitro study used finely powdered form of Pudina, Tulsi and Curry leaves extracts (Hakim Chi Chi Pharmacy, Surat, India) without any added preservatives. The test microorganism S. mutans was isolated from human saliva using mitis salivarius agar. The Minimum Inhibition Concentration (MIC) was determined using agar well diffusion for Group 1 (Tulsi), Group 2 (Pudina), Group 3 (Curry leaves) and Group 4 (Chlorhexidine). Using this MIC, modified Direct Contact Test was performed for specific evaluation of the antimicrobial efficacy of each product. Chlorhexidine (0.2%) was used as a positive control for the comparison of each product. The results of modified direct contact test were subjected to ANOVA Test and Tukey’s Test using SPSS software version 23.0 which showed that when compared against chlorhexidine, the antimicrobial efficacy against S. mutans in decreasing order was of curry leaves, tulsi and pudina and the result of the study was significant. Results: Curry leaves showed higher antimicrobial efficacy than tulsi and pudina when compared against chlorhexidine (control) and the results were statistically significant (p<0.05). Conclusion: Curry leaf extract shows promising antimicrobial property against S. mutans and can be recommended for caries control with further investigations.

Comparative evaluation of the antimicrobial efficacy of three endodontic sealers against Enterococcus faecalis with and without incorporation of Chitosan nanoparticles

2019 ◽  
Vol 11 (1) ◽  
pp. 21-28
Author(s):  
Dr. Rajisha K R
Keyword(s):  
Growth Inhibition ◽  
Enterococcus Faecalis ◽  
Direct Contact ◽  
Antibacterial Effect ◽  
Chitosan Nanoparticles ◽  
Contact Test ◽  
Agar Diffusion Test ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Direct Contact Test

Aims and Objectives: The aim of the present study was to evaluate and to compare the antibacterial activity of AH Plus, Tubliseal and MTA Fillapex against E.faecalis with and without incorporation of Chitosan nanoparticles. Methodology: The antibacterial effect against Enterococcus faecalis was evaluated by i) direct contact test (DCT) and ii) agar diffusion test (ADT). The nanoparticles were incorporated into the sealers at concentration of 2 % weight/ weight. Bacterial growth rates were analysed using ANOVA followed by Fischer Exact Test Results: The antibacterial tests demonstrated significant E.faecalis growth inhibition in the DCT with the sealer samples incorporated with chitosan nanoparticles. Direct contact test showed total growth inhibition of up to 8 logs in count in AH Plus, up to 7 logs in count in Tubliseal, and up to 6 logs in count in MTA Fillapex which were incorporated with chitosan nanoparticles. Significant differences were found between the unmodified sealers and the modified sealers. No antibacterial effect was observed in the ADT, indicating Chitosan nanoparticles were not diffusing into the agar. Conclusion: The endodontic sealers incorporating Chitosan nanoparticles exhibited significant and stable antimicrobial properties.

scholarly journals ANTIMICROBIAL ACTIVITY OF MINERAL TRIOXIDE AGGREGATE AND CALCIUM HYDROXIDE SEALER ON ENTEROCOCCUS FAECALIS STRAIN ATCC29212

2019 ◽  
pp. 123-125 ◽  
Author(s):  
TARA PRATHITA ◽  
NILA KESUMA DJAUHARIE ◽  
RATNA MEIDYAWATI
Keyword(s):  
Antimicrobial Activity ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Direct Contact ◽  
Mineral Trioxide Aggregate ◽  
Antimicrobial Effect ◽  
Time Points ◽  
Contact Test ◽  
Direct Contact Test

Objective: The objective of this study was to evaluate the antimicrobial activity of mineral trioxide aggregate (MTA) and calcium hydroxide (Ca(OH)2)sealers on Enterococcus faecalis at various time points after the preparation of the sealer.Methods: Antimicrobial activity of MTA and Ca(OH)2 sealer on E. faecalis (ATCC 29212) was assessed by the direct contact test immediately, 1 day,and 7 days after the preparation of the sealer.Results: The antimicrobial effect of MTA sealer was greatest 1 and 7 days after the preparation of the sealer. The effect of Ca(OH)2 was greatest 1 dayafter the preparation of the sealer.Conclusion: MTA sealer showed constant antimicrobial activity on E. faecalis overtime. By contrast, Ca(OH)2 sealer showed decreasing antimicrobialactivity overtime, with the greatest antimicrobial activity being 1 day after the preparation of the sealer.

Antibacterial Properties Evaluation of a Novel Polyurethane-Based Root Canal Sealer

2014 ◽  
Vol 804 ◽  
pp. 231-234
Author(s):  
Jian Wang ◽  
Ming Hui Zhao ◽  
Yu Bao Li ◽  
Yi Zuo ◽  
Bin Sun ◽  
...  
Keyword(s):  
Root Canal ◽  
Direct Contact ◽  
Antibacterial Properties ◽  
Adhesion Assay ◽  
Root Canal Sealer ◽  
Contact Test ◽  
Silver Phosphate ◽  
Application Potential ◽  
Rate Test ◽  
Direct Contact Test

Elimination of microorganisms from the root canal system and the prevention of subsequent reinfection are of importance for long-term endodontic treatment. The application of a sealer with antibacterial properties may reduce the reinfection and improve the success rate of the root filling treatment. The aim of this paper is to evaluate the antibacterial properties of a novel root canal sealer based on injectable self-curing polyurethane with silver phosphate (PU/Ag3PO4). The antibacterial abilities were assessed by direct contact test, anti-bacterial adhesion assay and bacteriostatic rate test. The results show that the fabricated PU/Ag3PO4sealer can completely inhibit the bacterial growth and prevent bacterial adhension effectively. The bacteriostatic rate is 93.6% and 98.1% when the bacteria incubated with PU/Ag3PO4 sealer for 7 and 24h respectively. The strong antibacterial abilities suggest that PU/Ag3PO4 sealer has great application potential in the field of root canal filling.Key words: Root canal sealer, polyurethane, silver phosphate, antibacterial properties, direct contact test

Antibacterial investigation of titanium-copper alloys using luminescent Staphylococcus epidermidis in a direct contact test

2019 ◽  
Vol 97 ◽  
pp. 707-714 ◽  
Author(s):  
Lee Fowler ◽  
Oscar Janson ◽  
Håkan Engqvist ◽  
Susanne Norgren ◽  
Caroline Öhman-Mägi
Keyword(s):  
Staphylococcus Epidermidis ◽  
Direct Contact ◽  
Copper Alloys ◽  
Contact Test ◽  
Direct Contact Test

Direct Contact Test for Evaluating Bacterial Growth on Machined and Rough Surface Implants

2017 ◽  
Vol 26 (6) ◽  
pp. 899-903 ◽  
Author(s):  
Shimshon Slutzkey ◽  
Ofer Moses ◽  
Haim Tal ◽  
Avi Meirowitz ◽  
Shlomo Matalon
Keyword(s):  
Rough Surface ◽  
Bacterial Growth ◽  
Direct Contact ◽  
Contact Test ◽  
Direct Contact Test
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