scholarly journals In VitroEvaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials againstEnterococcus faecalis,Candida albicans, andStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Duddi Narendra Nirupama ◽  
Mohan Thomas Nainan ◽  
Rajendran Ramaswamy ◽  
Sethumadhavan Muralidharan ◽  
Hulimangala Hosakote Lingareddy Usha ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Direct Contact ◽  
Microbial Growth ◽  
Antimicrobial Property ◽  
Recurrent Infection ◽  
Antimicrobial Effect ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Root Canal Sealers ◽  
Direct Contact Test

Root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different microorganisms,E. faecalis, C. albicans, andS. aureus, by direct contact test. 10 μL microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°C. Subsequently microbial growth was measured spectrophotometrically every 30 min for 18 hours. The microbial suspensions were simultaneously tested to determine the antimicrobial effect of components which are capable of diffusing into the medium. The results revealed that AH Plus and iRootSP had significantly higher antimicrobial activity againstE. faecalis. AH Plus and Tubliseal EWT showed significantly higher antimicrobial activity againstC. albicansandS. aureuscompared to iRoot SP and EndoRez. EndoRez showed the least antimicrobial activity against all the three microorganisms. Inhibition of microbial growth is related to the direct contact of microorganisms with the sealers. In conclusion AH Plus had significantly higher antimicrobial activity againstE. faecalis, C. albicans, andS. aureus.

Comparative evaluation of the antimicrobial efficacy of three endodontic sealers against Enterococcus faecalis with and without incorporation of Chitosan nanoparticles

2019 ◽  
Vol 11 (1) ◽  
pp. 21-28
Author(s):  
Dr. Rajisha K R
Keyword(s):  
Growth Inhibition ◽  
Enterococcus Faecalis ◽  
Direct Contact ◽  
Antibacterial Effect ◽  
Chitosan Nanoparticles ◽  
Contact Test ◽  
Agar Diffusion Test ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Direct Contact Test

Aims and Objectives: The aim of the present study was to evaluate and to compare the antibacterial activity of AH Plus, Tubliseal and MTA Fillapex against E.faecalis with and without incorporation of Chitosan nanoparticles. Methodology: The antibacterial effect against Enterococcus faecalis was evaluated by i) direct contact test (DCT) and ii) agar diffusion test (ADT). The nanoparticles were incorporated into the sealers at concentration of 2 % weight/ weight. Bacterial growth rates were analysed using ANOVA followed by Fischer Exact Test Results: The antibacterial tests demonstrated significant E.faecalis growth inhibition in the DCT with the sealer samples incorporated with chitosan nanoparticles. Direct contact test showed total growth inhibition of up to 8 logs in count in AH Plus, up to 7 logs in count in Tubliseal, and up to 6 logs in count in MTA Fillapex which were incorporated with chitosan nanoparticles. Significant differences were found between the unmodified sealers and the modified sealers. No antibacterial effect was observed in the ADT, indicating Chitosan nanoparticles were not diffusing into the agar. Conclusion: The endodontic sealers incorporating Chitosan nanoparticles exhibited significant and stable antimicrobial properties.

scholarly journals ANTIMICROBIAL ACTIVITY OF MINERAL TRIOXIDE AGGREGATE AND CALCIUM HYDROXIDE SEALER ON ENTEROCOCCUS FAECALIS STRAIN ATCC29212

2019 ◽  
pp. 123-125 ◽  
Author(s):  
TARA PRATHITA ◽  
NILA KESUMA DJAUHARIE ◽  
RATNA MEIDYAWATI
Keyword(s):  
Antimicrobial Activity ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Direct Contact ◽  
Mineral Trioxide Aggregate ◽  
Antimicrobial Effect ◽  
Time Points ◽  
Contact Test ◽  
Direct Contact Test

Objective: The objective of this study was to evaluate the antimicrobial activity of mineral trioxide aggregate (MTA) and calcium hydroxide (Ca(OH)2)sealers on Enterococcus faecalis at various time points after the preparation of the sealer.Methods: Antimicrobial activity of MTA and Ca(OH)2 sealer on E. faecalis (ATCC 29212) was assessed by the direct contact test immediately, 1 day,and 7 days after the preparation of the sealer.Results: The antimicrobial effect of MTA sealer was greatest 1 and 7 days after the preparation of the sealer. The effect of Ca(OH)2 was greatest 1 dayafter the preparation of the sealer.Conclusion: MTA sealer showed constant antimicrobial activity on E. faecalis overtime. By contrast, Ca(OH)2 sealer showed decreasing antimicrobialactivity overtime, with the greatest antimicrobial activity being 1 day after the preparation of the sealer.

scholarly journals In vitro evaluation of the antimicrobial activity of five root canal sealers

2004 ◽  
Vol 15 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Brenda Paula Figueiredo de Almeida Gomes ◽  
José Assis Pedroso ◽  
Rogério Castilho Jacinto ◽  
Morgana Eli Vianna ◽  
Caio Cezar Randi Ferraz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Contact Method ◽  
Streptococcus Sanguis ◽  
Actinomyces Naeslundii ◽  
Agar Diffusion Test ◽  
Significant Difference ◽  
Ah Plus ◽  
Endodontic Sealers

The aim of the present study was to analyze the antimicrobial properties of five endodontic sealers: Endo Fill, Endomethasone, Endomethasone N, Sealer 26 and AH-Plus, against the following microorganisms: Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus sanguis and Actinomyces naeslundii. The sealers were tested immediately, 24 h, 48 h and 7 days after manipulation.The direct contact method through the observation of the microbial growth in liquid medium and the agar diffusion test were used to evaluate the antimicrobial properties of the sealers. The results, in both methodologies used, showed that immediately after manipulation, Endo-Fill and Endomethasone demonstrated the highest antimicrobial activity, with no statistically significant difference between them. Sealer 26 demonstrated the lowest antimicrobial activity. At all other times after manipulation, there were no statistically significant differences among all the sealers tested. In conclusion, none of the sealers totally inhibited the growth of the microorganisms. Furthermore, the antimicrobial activity of each sealer decreased with time and was dependent upon the microbial susceptibility to them.

scholarly journals Antimicrobial activity of endodontic sealers and medications containing chitosan and silver nanoparticles against Enterococcus faecalis

2019 ◽  
Vol 17 (3) ◽  
pp. 228080001985177 ◽  
Author(s):  
Juan Pablo Loyola-Rodríguez ◽  
Fernando Torres-Méndez ◽  
León Francisco Espinosa-Cristobal ◽  
José Obed García-Cortes ◽  
Alejandra Loyola-Leyva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Enterococcus Faecalis ◽  
Membrane Surface ◽  
Chemical Components ◽  
Collagen Membrane ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Antimicrobial Effectiveness

Background: The main microorganism associated with the failure of endodontic treatments is Enterococcus faecalis. Although several endodontic therapeutics have demonstrated antimicrobial activity against E. faecalis, the antimicrobial effectiveness of chitosan (CsNPs) and silver nanoparticles (AgNPs) included into conventional endodontic sealers for endodontic therapies is still unclear. Aim: The objective of this study was to evaluate the antibacterial activity increment (AAI) of endodontic sealers containing CsNPs and AgNPs as well as some chemical components against E. faecalis by direct contact assays. Methods: CsNPs and AgNPs were synthesized by reduction and ionic gelation methods, respectively. Nanoparticles were characterized by dynamic light scattering and energy dispersive X-ray analysis. The bactericidal activity was tested on monolayers on agar plates and collagen membrane surface assays against E. faecalis. Results: The size of CsNPs was 70.6±14.8 nm and zeta potential was 52.0±5.4 mV; the size of AgNPs was 54.2±8.5 nm, and zeta potential was –48.4±6.9 mV. All materials, single or combined, showed an AAI, especially when CsNPs, chlorhexidine (Chx), and the combination of CsNPs-Chx were added. However, the combination of CsNPs-Chx showed the highest (55%) AAI, followed by Chx (35.5%) and CsNPs (11.1%), respectively. There was a significant statistical difference in all comparisons ( p < 0.05). Tubliseal (40%) and AH Plus (32%) sealants showed a higher AAI on E. faecalis in the monolayer test and collagen membrane assay analyzed by scanning electron microscopy. Conclusions: Tubliseal and AH plus sealers combined with nanoparticles, especially CsNPs-Chx, could be used for conventional endodontic treatments in the control of E. faecalis bacteria.

An In vitro Evaluation of the Antimicrobial Activity of Nine Root Canal Sealers

2008 ◽  
Vol 9 (5) ◽  
pp. 60-67
Author(s):  
Leena Smadi ◽  
Azmi Mahafzah ◽  
Ameen Khraisat
Keyword(s):  
Antimicrobial Activity ◽  
Root Canal ◽  
Bacterial Strains ◽  
A Value ◽  
Agar Diffusion Test ◽  
Ah Plus ◽  
Microbial Strains ◽  
And Control ◽  
Root Canal Sealers

Abstract Aim The purpose of this in vitro study was to analyze the antimicrobial activity of root canal sealers by using the agar diffusion test (ADT). Methods and Materials Three categories of root canal sealers were included in the study: resin-based sealers (4), zinc oxide-based sealers (3), and calcium hydroxide-based sealers (2). The microbial strains used were: S. aureus (2 strains), C. albicans (2 strains), and E. faecalis (1 strain). Statistical analysis was conducted using a one-way analysis of variance (ANOVA). Tests of differences were analyzed using the Tukey's test with a value of p < 0.05 considered statistically significant. Results The antimicrobial activity of root canal sealers was ranked in descending order as follows: Sealite Regular, Cortisemol, Dentalis KEZ, AH26, Sealapex, Acroseal/Topseal, and Endorez/AH plus. Conclusion Root canal sealers showed different inhibitory effects depending on their types and the bacterial strains tested. Root canal sealers containing formaldehyde and eugenol proved to be effective against the microorganisms studied. Clinical Significance The incorporation of antimicrobial components into root canal sealers may become an essential factor in preventing the re-growth of residual bacteria and control of bacteria re-entry into the root canal system. Citation Smadi L, Mahafzah A, Khraisat A. An In vitro Evaluation of the Antimicrobial Activity of Nine Root Canal Sealers. J Contemp Dent Pract 2008 July; (9)5:060-067.

scholarly journals In vitro evaluation of the antimicrobial activity of endodontic sealers

2006 ◽  
Vol 20 (4) ◽  
pp. 303-306 ◽  
Author(s):  
Daniela Cristina Miyagak ◽  
Elaine Manso Oliveira Franco de Carvalho ◽  
Carlos Roberto Colombo Robazza ◽  
Jorge Kleber Chavasco ◽  
Gustavo Labegalline Levorato
Keyword(s):  
Antimicrobial Activity ◽  
Portland Cement ◽  
Mineral Trioxide Aggregate ◽  
Diffusion Method ◽  
E Coli ◽  
Microbial Inhibition ◽  
Ah Plus ◽  
Inhibition Zones ◽  
Endodontic Sealers

The purpose of this study is to evaluate the antimicrobial activity of the endodontic sealers: N-Rickert, Sealapex, AH Plus, Mineral Trioxide Aggregate (MTA) and portland cement. The Agar diffusion method was used in plates previously inoculated with the following microorganisms: C. albicans, S. aureus, E. faecalis, E. coli. The diameters of microbial inhibition zones were measured after 24 hours of incubation in kiln at 37°C. According to the methodology used, it was possible to conclude that only the sealers AH Plus and N-Rickert presented antimicrobial activity against C. albicans, S. aureus, and E. coli; no antimicrobial activity in MTA, Sealapex and portland cement was observed. N-Rickert presented the largest inhibition zones varying from 8 to 18 mm, and the microorganism E. faecalis was resistant against all sealers tested.

scholarly journals Component Release and Mechanical Properties of Endodontic Sealers following Incorporation of Antimicrobial Agents

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Elizabeta S. Gjorgievska ◽  
John W. Nicholson ◽  
Nichola J. Coleman ◽  
Samantha Booth ◽  
Aleksandar Dimkov ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Antimicrobial Agents ◽  
Cetylpyridinium Chloride ◽  
Antimicrobial Properties ◽  
Cure Reaction ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Root Canal Sealers ◽  
Ammonium Compounds ◽  
Antimicrobial Additive

Root canal sealers with antimicrobial activity are highly beneficial; therefore, their antimicrobial properties could be improved by incorporation of antimicrobial agents. In the present study, the release of the quaternary ammonium compounds from endodontic sealers admixed with either benzalkonium chloride (BC) or cetylpyridinium chloride (CPC) at loadings of 2% wt was monitored. The effect of these additives on the compressive strengths and their release from the sealers was determined after 1 and 4 weeks. All of the materials studied were found to be capable of releasing antimicrobial additive in useful quantities. The release of CPC occurred to a statistically significant greater extent than BC for all materials. The addition of both BC and CPC generally decreased the compressive strength of all the endodontic sealers, with the exception of CPC in AH Plus, where the compressive strength was significantly increased. This suggests that, for these endodontic sealers, the antimicrobial additives alter the setting chemistry. AH Plus is an epoxy-based material cured with an amine, and in this case the increase in compressive strength with CPC is attributed to an enhanced cure reaction with this system. In all other cases, the additive inhibited the cure reaction to a greater or lesser extent.

scholarly journals Antimicrobial and cytotoxic capacity of pyroligneous extracts films of Eucalyptus grandis and chitosan for oral applications

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Juliana Leitzke Santos de Souza ◽  
Tomaz Alves ◽  
Laísa Camerini ◽  
Fernanda Nedel ◽  
Angela Diniz Campos ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Dental Caries ◽  
Direct Contact ◽  
Eucalyptus Grandis ◽  
Effective Control ◽  
Natural Polymers ◽  
Contact Test ◽  
Enamel Demineralization ◽  
Chitosan Films ◽  
Direct Contact Test

AbstractChitosan films containing distilled pyroligneous extracts of Eucalyptus grandis (DPEC), characterized and developed by Brazilian Agricultural Research Corporation—Embrapa Temperate Agriculture (EMBRAPA-CPACT), were evaluated for antimicrobial activity against Candida albicans, Streptococcus mutans, and Lactobacillus acidophilus by direct contact test. Further, their capacity for the prevention of teeth enamel demineralization and cytotoxicity in vitro were also determined. The natural polymers were tested at different concentrations (1500–7500 µg mL−1) and the formulation of an experimental fluoride varnish with antimicrobial activity was evaluated by direct contact test, whereas cytotoxicity was analyzed through the colorimetric MTT assay. Preliminary data showed no statistically significant differences in cytotoxicity to NIH/3T3 cell line when DPEC is compared to the control group. On the other hand, the antimicrobial capacity and demineralization effects were found between the test groups at the different concentrations tested. Chitosan films containing distilled pyroligneous extracts of E. grandis may be an effective control strategy to prevent biofilm formation related to dental caries when applied as a protective varnish. They may inhibit the colonization of oral microorganisms and possibly control dental caries through a decrease in pH and impairment of enamel demineralization.

scholarly journals An Assessment of Antibacterial Activity of Four Endodontic Sealers on Enterococcus faecalis by a Direct Contact Test: An In Vitro Study

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Lavanya Anumula ◽  
Swaroop Kumar ◽  
Venkata Suneel Kumar ◽  
Chandra Sekhar ◽  
Murali Krishna ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Direct Contact ◽  
Glass Ionomer ◽  
Polydimethyl Siloxane ◽  
Contact Test ◽  
Urethane Dimethacrylate ◽  
Endodontic Sealers ◽  
Direct Contact Test

Aim. To evaluate the antibacterial activity of four endodontic sealers on Enterococcus faecalis by a direct contact test. Material and Methods. Enterococcus faecalis was used as a test organism. Direct contact test which is based on measuring the effect of close contact between test bacteria and tested material on the kinetics of bacterial growth was performed to overcome the disadvantages of agar diffusion test. The sealers tested were zinc oxide eugenol-based sealer, glass-ionomer-based sealer, polydimethyl-siloxane-based sealer, and urethane dimethacrylate resin-based sealer. Data was collected by recording the optical density with the help of a spectrophotometer. Results. The sealers exhibited different inhibitory effects. The results obtained were subjected to statistical analysis by Kruskal Wallis analysis of variance and Dunn's multiple comparison test. Group comparison showed very highly significant difference between the groups. Conclusion. Zinc oxide eugenol-based sealer was the most effective and urethane dimethacrylate resin-based sealer was the least effective against Enterococcus faecalis, whereas glass-ionomer-based and polydimethyl-siloxane-based sealers were effective only for a short period. Inhibition of the bacterial growth is related to the direct contact of the microorganism with the sealer.

Sign in / Sign up

Export Citation Format

Share Document