Losartan preserves glomerular basement membrane anionic charge sites in a rat model of nephropathy

2012 ◽  
Vol 26 (4) ◽  
pp. 660-666 ◽  
Author(s):  
Yan Liu ◽  
Daoyuan Zhou ◽  
Xiao Xiao ◽  
Danping Qin ◽  
Xiaoshi Zhong ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Rat Model ◽  
Glomerular Basement Membrane ◽  
Anionic Charge

The effect of losartan and captopril on glomerular basement membrane anionic charge in a diabetic rat model

1999 ◽  
Vol 17 (8) ◽  
pp. 1217-1223 ◽  
Author(s):  
Dilek G. Yavuz ◽  
Önder Ersöz ◽  
Belgin Kuçükkaya ◽  
Yasemin Budak ◽  
Rengin Ahiskali ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Rat Model ◽  
Glomerular Basement Membrane ◽  
Diabetic Rat ◽  
Anionic Charge ◽  
Diabetic Rat Model

scholarly journals Anionic charge concentration of rat kidney glomeruli and glomerular basement membrane

1993 ◽  
Vol 289 (3) ◽  
pp. 647-652 ◽  
Author(s):  
W D Comper ◽  
A S N Lee ◽  
M Tay ◽  
Y Adal
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Electrostatic Interactions ◽  
Enzyme Inhibitors ◽  
Rat Kidney ◽  
Basement Membranes ◽  
Sulphate Content ◽  
Anionic Charge ◽  
Charge Concentration ◽  
A Charge

Estimates of levels of glomerular and glomerular-basement-membrane anion charge should serve as useful quantitative markers for the integrity of the tissues in health and disease. We have developed a simple, rapid, technique to measure this charge through the use of ion exchange with radioisotopes 22Na+ and 36Cl- at low ionic strengths in phosphate buffer. When this technique is used, normal glomeruli isolated from rat have a measured net anion charge concentration of 17.4 +/- 3.7 p-equiv. per glomerulus (n = 20). Perfused rat kidneys that lose approximately half of their glomerular heparan [35S]sulphate content (owing to oxygen-radical damage) exhibited a lower anion charge, of 7.5 +/- 1.6 p-equiv. per glomerulus (n = 5). Glomerular basement membranes prepared from rat glomeruli by a sonication-centrifugation procedure in the presence of enzyme inhibitors had a charge concentration of 6.3 +/- 0.7 mu-equiv./g wet wt. of tissue (n = 4), whereas membranes prepared by sonication, centrifugation, DNAse and detergent treatment had a charge concentration of 7.1 +/- 1.6 mu-equiv./g wet wt. (n = 4). Isotope-dilution experiments with 3H2O on these detergent-prepared glomerular basement membranes demonstrated that they had a water content of approx. 93%, which would then give a net anion charge concentration of 7.6 +/- 1.7 m-equiv./l (n = 4). These values are in good agreement with those obtained by others using titration techniques [Bray and Robinson (1984) Kidney Int. 25, 527-533]. The relatively low magnitude of glomerular anion charge in normal kidneys is consistent with other recent findings that glomerular anion charge is too low to affect the glomerular transport of charged molecules in a direct, passive, biophysical manner through electrostatic interactions.

scholarly journals Analysis of anionic charge of the glomerular basement membrane in aminonucleoside nephrosis

1989 ◽  
Vol 35 (6) ◽  
pp. 1405-1408 ◽  
Author(s):  
Shuzo Kobayashi ◽  
Mitsumasa Nagase ◽  
Nishio Honda ◽  
Kyoko Adachi ◽  
Norio Ichinose ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Anionic Charge ◽  
Aminonucleoside Nephrosis

Correlation between Histological Changes and Loss of Anionic Charge of the Glomerular Basement Membrane in Early-Onset Pre-Eclampsia

Nephron ◽  
1997 ◽  
Vol 75 (2) ◽  
pp. 201-207 ◽  
Author(s):  
T. Naicker ◽  
I.G.H. Randeree ◽  
J. Moodley ◽  
S.M. Khedun ◽  
R. Ramsaroop ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Early Onset ◽  
Histological Changes ◽  
Anionic Charge

Antiproteinuric effect of pirfenidone in a rat model of anti-glomerular basement membrane glomerulonephritis

2014 ◽  
Vol 737 ◽  
pp. 106-116 ◽  
Author(s):  
Koji Takakura ◽  
Kazuhiko Mizukami ◽  
Hikaru Mitori ◽  
Takahisa Noto ◽  
Yuichi Tomura
Keyword(s):  
Basement Membrane ◽  
Rat Model ◽  
Glomerular Basement Membrane ◽  
Antiproteinuric Effect

scholarly journals Osteopontin Overproduction Is Associated with Progression of Glomerular Fibrosis in a Rat Model of Anti-Glomerular Basement Membrane Glomerulonephritis

2010 ◽  
Vol 32 (3) ◽  
pp. 262-271 ◽  
Author(s):  
Justin Merszei ◽  
Jean Wu ◽  
Lisa Torres ◽  
John M. Hicks ◽  
Todd Bartkowiak ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Rat Model ◽  
Glomerular Basement Membrane

scholarly journals The Glomerular Basement Membrane as a Model System to Study the Bioactivity of Heparan Sulfate Glycosaminoglycans

2012 ◽  
Vol 18 (1) ◽  
pp. 3-21 ◽  
Author(s):  
Kevin J. McCarthy ◽  
Deborah J. Wassenhove-McCarthy
Keyword(s):  
Basement Membrane ◽  
Heparan Sulfate ◽  
Glomerular Basement Membrane ◽  
Molecular Genetic ◽  
Research Area ◽  
Critical Elements ◽  
Core Proteins ◽  
Transmembrane Flux ◽  
Anionic Charge ◽  
A Charge

AbstractThe glomerular basement membrane and its associated cells are critical elements in the renal ultrafiltration process. Traditionally the anionic charge associated with several carbohydrate moieties in the glomerular basement membrane are thought to form a charge selective barrier that restricts the transmembrane flux of anionic proteins across the glomerular basement membrane into the urinary space. The charge selective function, along with the size selective component of the basement membrane, serves to limit the efflux of plasma proteins from the capillary lumen. Heparan sulfate glycosaminoglycans are anionically charged carbohydrate structures attached to proteoglycan core proteins and have a role in establishing the charge selective function of the glomerular basement membrane. Although there are a large number of studies in the literature that support this concept, the results of several recent studies using molecular genetic approaches to minimize the anionic charge of the glomerular basement membrane would suggest that the role of heparan sulfate glycosaminoglycans in the glomerular capillary wall are still not yet entirely resolved, suggesting that this research area still requires new and novel exploration.

Electron microscopic radioautographic studies on the incorporation of 3H proline in the glomerular basement membrane (GBM) in antistreptococcal-cell-membrane (SCM) injected mice

1984 ◽  
Vol 42 ◽  
pp. 188-189
Author(s):  
R.P. Nayyar ◽  
C.F. Lange ◽  
J. L. Borke
Keyword(s):  
Body Weight ◽  
Cell Membrane ◽  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Mesangial Matrix ◽  
Electron Microscopic ◽  
Group A ◽  
Injection Protocol

Streptococcal cell membrane (SCM) antiserum injected mice show a significant thickening of glomerular basement membrane (GBM) and an increase in mesangial matrix within 4 to 24 hours of antiserum administration (1,2,3). This study was undertaken to evaluate the incorporation of 3H proline into glomerular cells and GBM under normal and anti-SCM induced conditions. Mice were administered, intraperitoneally, 0.1 ml of normal or anti-SCM serum followed by a 10 µC/g body weight injection of 3H proline. Details of the preparation of anti-SCM (Group A type 12 streptococcal pyogenes) and other sera and injection protocol have been described elsewhere (2). After 15 minutes of isotope injection a chase of cold proline was given and animal sacrificed at 20 minutes, 1,2,4,8,24 and 48 hours. One of the removed kidneys was processed for immunofluorescence, light and electron microscopic radioautographic studies; second kidney was used for GBM isolation and aminoacid analysis.

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