Correlation between Histological Changes and Loss of Anionic Charge of the Glomerular Basement Membrane in Early-Onset Pre-Eclampsia

Nephron ◽  
1997 ◽  
Vol 75 (2) ◽  
pp. 201-207 ◽  
Author(s):  
T. Naicker ◽  
I.G.H. Randeree ◽  
J. Moodley ◽  
S.M. Khedun ◽  
R. Ramsaroop ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Early Onset ◽  
Histological Changes ◽  
Anionic Charge

scholarly journals Anionic charge concentration of rat kidney glomeruli and glomerular basement membrane

1993 ◽  
Vol 289 (3) ◽  
pp. 647-652 ◽  
Author(s):  
W D Comper ◽  
A S N Lee ◽  
M Tay ◽  
Y Adal
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Electrostatic Interactions ◽  
Enzyme Inhibitors ◽  
Rat Kidney ◽  
Basement Membranes ◽  
Sulphate Content ◽  
Anionic Charge ◽  
Charge Concentration ◽  
A Charge

Estimates of levels of glomerular and glomerular-basement-membrane anion charge should serve as useful quantitative markers for the integrity of the tissues in health and disease. We have developed a simple, rapid, technique to measure this charge through the use of ion exchange with radioisotopes 22Na+ and 36Cl- at low ionic strengths in phosphate buffer. When this technique is used, normal glomeruli isolated from rat have a measured net anion charge concentration of 17.4 +/- 3.7 p-equiv. per glomerulus (n = 20). Perfused rat kidneys that lose approximately half of their glomerular heparan [35S]sulphate content (owing to oxygen-radical damage) exhibited a lower anion charge, of 7.5 +/- 1.6 p-equiv. per glomerulus (n = 5). Glomerular basement membranes prepared from rat glomeruli by a sonication-centrifugation procedure in the presence of enzyme inhibitors had a charge concentration of 6.3 +/- 0.7 mu-equiv./g wet wt. of tissue (n = 4), whereas membranes prepared by sonication, centrifugation, DNAse and detergent treatment had a charge concentration of 7.1 +/- 1.6 mu-equiv./g wet wt. (n = 4). Isotope-dilution experiments with 3H2O on these detergent-prepared glomerular basement membranes demonstrated that they had a water content of approx. 93%, which would then give a net anion charge concentration of 7.6 +/- 1.7 m-equiv./l (n = 4). These values are in good agreement with those obtained by others using titration techniques [Bray and Robinson (1984) Kidney Int. 25, 527-533]. The relatively low magnitude of glomerular anion charge in normal kidneys is consistent with other recent findings that glomerular anion charge is too low to affect the glomerular transport of charged molecules in a direct, passive, biophysical manner through electrostatic interactions.

Losartan preserves glomerular basement membrane anionic charge sites in a rat model of nephropathy

2012 ◽  
Vol 26 (4) ◽  
pp. 660-666 ◽  
Author(s):  
Yan Liu ◽  
Daoyuan Zhou ◽  
Xiao Xiao ◽  
Danping Qin ◽  
Xiaoshi Zhong ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Rat Model ◽  
Glomerular Basement Membrane ◽  
Anionic Charge

Early onset calciphylaxis following acute kidney injury secondary to anti-glomerular basement membrane antibody disease

2021 ◽  
Vol 14 (4) ◽  
pp. e241265
Author(s):  
Sheikh Raza Shahzad ◽  
Faris Alfaris ◽  
Mustafa Erdem Arslan ◽  
Swati Mehta
Keyword(s):  
Acute Kidney Injury ◽  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Early Onset ◽  
Kidney Injury ◽  
Caucasian Woman ◽  
History Of ◽  
Stage Renal Disease ◽  
End Stage

Calciphylaxis is commonly associated with end-stage renal disease (ESRD) and renal transplant. We present a rare case of early onset calciphylaxis in a patient presenting with acute kidney injury (AKI) secondary to anti-glomerular basement membrane (anti-GBM) antibody disease. A 65-year-old obese Caucasian woman with type 2 diabetes mellitus and hypertension presented with a 1-month history of painless gross haematuria and worsening lower extremity oedema. Laboratory results indicated AKI and nephrotic-range proteinuria. Anti-glomerular antibodies were elevated. Renal biopsy revealed focal crescentic glomerulonephritis with linear capillary immunoglobulin G staining consistent with anti-GBM antibody disease. She was treated with haemodialysis, plasmapheresis, steroids, bumetanide and cyclophosphamide. Two months later, she developed necrotic lesions on bilateral thighs. Wound biopsy was consistent with calciphylaxis. This case highlights that calciphylaxis, usually seen in patients with chronic kidney disease or ESRD, can manifest in patients with AKI as well.

scholarly journals Analysis of anionic charge of the glomerular basement membrane in aminonucleoside nephrosis

1989 ◽  
Vol 35 (6) ◽  
pp. 1405-1408 ◽  
Author(s):  
Shuzo Kobayashi ◽  
Mitsumasa Nagase ◽  
Nishio Honda ◽  
Kyoko Adachi ◽  
Norio Ichinose ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Anionic Charge ◽  
Aminonucleoside Nephrosis

scholarly journals A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods

2022 ◽  
Vol 23 (1) ◽  
Author(s):  
Débora Leal Viana ◽  
Dona Jeanne Alladagbin ◽  
Washington L. C. dos-Santos ◽  
Claudio Pereira Figueira
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Disease Diagnosis ◽  
Brazilian Population ◽  
Membrane Thickness ◽  
Histological Changes ◽  
Basement Membrane Thickness ◽  
Shortest Distance ◽  
Sources Of Variation ◽  
Glomerular Basement Membrane Thickness

Abstract Introduction Here we report estimates of glomerular basement membrane (GBM) thickness in the Brazilian population performed using direct (DM) and orthogonal interception methods (OIM), and comment on potential sources of variation among estimates made by different laboratories. Methodology A total of 38 patients, ranging from 3 to 78 years of age, 26 (68%) males and 12 (32%) females, were submitted to kidney biopsy procedures for renal disease diagnosis. Glomeruli were diagnosed with minor histological changes by conventional, immunofluorescence and electron microscopy. GBM thickness was estimated using both DM and OIM methods. Results Estimates of GBM thickness obtained using DM were higher than those obtained by OIM. However, the application of a correction for non-perpendicular membrane sectioning to DM estimates yielded similar results to those obtained under OIM. The estimated GMB thickness using DM after correction was 289 + 44 nm, versus 287 + 48 nm by OIM. No statistically significant differences were detected in GMB thickness, nor with respect to patient age or sex. Conclusions GBM thickness in the studied Brazilian population measured approximately 290 nm. The application of criteria for estimating the shortest distance between the endothelial and podocyte cell membranes with correction for non-perpendicular membrane sectioning can increase the accuracy of GBM thickness estimates using DM and OIM.

scholarly journals The Glomerular Basement Membrane as a Model System to Study the Bioactivity of Heparan Sulfate Glycosaminoglycans

2012 ◽  
Vol 18 (1) ◽  
pp. 3-21 ◽  
Author(s):  
Kevin J. McCarthy ◽  
Deborah J. Wassenhove-McCarthy
Keyword(s):  
Basement Membrane ◽  
Heparan Sulfate ◽  
Glomerular Basement Membrane ◽  
Molecular Genetic ◽  
Research Area ◽  
Critical Elements ◽  
Core Proteins ◽  
Transmembrane Flux ◽  
Anionic Charge ◽  
A Charge

AbstractThe glomerular basement membrane and its associated cells are critical elements in the renal ultrafiltration process. Traditionally the anionic charge associated with several carbohydrate moieties in the glomerular basement membrane are thought to form a charge selective barrier that restricts the transmembrane flux of anionic proteins across the glomerular basement membrane into the urinary space. The charge selective function, along with the size selective component of the basement membrane, serves to limit the efflux of plasma proteins from the capillary lumen. Heparan sulfate glycosaminoglycans are anionically charged carbohydrate structures attached to proteoglycan core proteins and have a role in establishing the charge selective function of the glomerular basement membrane. Although there are a large number of studies in the literature that support this concept, the results of several recent studies using molecular genetic approaches to minimize the anionic charge of the glomerular basement membrane would suggest that the role of heparan sulfate glycosaminoglycans in the glomerular capillary wall are still not yet entirely resolved, suggesting that this research area still requires new and novel exploration.

The effect of losartan and captopril on glomerular basement membrane anionic charge in a diabetic rat model

1999 ◽  
Vol 17 (8) ◽  
pp. 1217-1223 ◽  
Author(s):  
Dilek G. Yavuz ◽  
Önder Ersöz ◽  
Belgin Kuçükkaya ◽  
Yasemin Budak ◽  
Rengin Ahiskali ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Rat Model ◽  
Glomerular Basement Membrane ◽  
Diabetic Rat ◽  
Anionic Charge ◽  
Diabetic Rat Model

Electron microscopic radioautographic studies on the incorporation of 3H proline in the glomerular basement membrane (GBM) in antistreptococcal-cell-membrane (SCM) injected mice

1984 ◽  
Vol 42 ◽  
pp. 188-189
Author(s):  
R.P. Nayyar ◽  
C.F. Lange ◽  
J. L. Borke
Keyword(s):  
Body Weight ◽  
Cell Membrane ◽  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Mesangial Matrix ◽  
Electron Microscopic ◽  
Group A ◽  
Injection Protocol

Streptococcal cell membrane (SCM) antiserum injected mice show a significant thickening of glomerular basement membrane (GBM) and an increase in mesangial matrix within 4 to 24 hours of antiserum administration (1,2,3). This study was undertaken to evaluate the incorporation of 3H proline into glomerular cells and GBM under normal and anti-SCM induced conditions. Mice were administered, intraperitoneally, 0.1 ml of normal or anti-SCM serum followed by a 10 µC/g body weight injection of 3H proline. Details of the preparation of anti-SCM (Group A type 12 streptococcal pyogenes) and other sera and injection protocol have been described elsewhere (2). After 15 minutes of isotope injection a chase of cold proline was given and animal sacrificed at 20 minutes, 1,2,4,8,24 and 48 hours. One of the removed kidneys was processed for immunofluorescence, light and electron microscopic radioautographic studies; second kidney was used for GBM isolation and aminoacid analysis.

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