scholarly journals Anionic charge concentration of rat kidney glomeruli and glomerular basement membrane

1993 ◽  
Vol 289 (3) ◽  
pp. 647-652 ◽  
Author(s):  
W D Comper ◽  
A S N Lee ◽  
M Tay ◽  
Y Adal
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Electrostatic Interactions ◽  
Enzyme Inhibitors ◽  
Rat Kidney ◽  
Basement Membranes ◽  
Sulphate Content ◽  
Anionic Charge ◽  
Charge Concentration ◽  
A Charge

Estimates of levels of glomerular and glomerular-basement-membrane anion charge should serve as useful quantitative markers for the integrity of the tissues in health and disease. We have developed a simple, rapid, technique to measure this charge through the use of ion exchange with radioisotopes 22Na+ and 36Cl- at low ionic strengths in phosphate buffer. When this technique is used, normal glomeruli isolated from rat have a measured net anion charge concentration of 17.4 +/- 3.7 p-equiv. per glomerulus (n = 20). Perfused rat kidneys that lose approximately half of their glomerular heparan [35S]sulphate content (owing to oxygen-radical damage) exhibited a lower anion charge, of 7.5 +/- 1.6 p-equiv. per glomerulus (n = 5). Glomerular basement membranes prepared from rat glomeruli by a sonication-centrifugation procedure in the presence of enzyme inhibitors had a charge concentration of 6.3 +/- 0.7 mu-equiv./g wet wt. of tissue (n = 4), whereas membranes prepared by sonication, centrifugation, DNAse and detergent treatment had a charge concentration of 7.1 +/- 1.6 mu-equiv./g wet wt. (n = 4). Isotope-dilution experiments with 3H2O on these detergent-prepared glomerular basement membranes demonstrated that they had a water content of approx. 93%, which would then give a net anion charge concentration of 7.6 +/- 1.7 m-equiv./l (n = 4). These values are in good agreement with those obtained by others using titration techniques [Bray and Robinson (1984) Kidney Int. 25, 527-533]. The relatively low magnitude of glomerular anion charge in normal kidneys is consistent with other recent findings that glomerular anion charge is too low to affect the glomerular transport of charged molecules in a direct, passive, biophysical manner through electrostatic interactions.

scholarly journals The Glomerular Basement Membrane as a Model System to Study the Bioactivity of Heparan Sulfate Glycosaminoglycans

2012 ◽  
Vol 18 (1) ◽  
pp. 3-21 ◽  
Author(s):  
Kevin J. McCarthy ◽  
Deborah J. Wassenhove-McCarthy
Keyword(s):  
Basement Membrane ◽  
Heparan Sulfate ◽  
Glomerular Basement Membrane ◽  
Molecular Genetic ◽  
Research Area ◽  
Critical Elements ◽  
Core Proteins ◽  
Transmembrane Flux ◽  
Anionic Charge ◽  
A Charge

AbstractThe glomerular basement membrane and its associated cells are critical elements in the renal ultrafiltration process. Traditionally the anionic charge associated with several carbohydrate moieties in the glomerular basement membrane are thought to form a charge selective barrier that restricts the transmembrane flux of anionic proteins across the glomerular basement membrane into the urinary space. The charge selective function, along with the size selective component of the basement membrane, serves to limit the efflux of plasma proteins from the capillary lumen. Heparan sulfate glycosaminoglycans are anionically charged carbohydrate structures attached to proteoglycan core proteins and have a role in establishing the charge selective function of the glomerular basement membrane. Although there are a large number of studies in the literature that support this concept, the results of several recent studies using molecular genetic approaches to minimize the anionic charge of the glomerular basement membrane would suggest that the role of heparan sulfate glycosaminoglycans in the glomerular capillary wall are still not yet entirely resolved, suggesting that this research area still requires new and novel exploration.

scholarly journals SARS-CoV-2 infection and recurrence of anti-glomerular basement disease: a case report

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Alexander Winkler ◽  
Emanuel Zitt ◽  
Hannelore Sprenger-Mähr ◽  
Afschin Soleiman ◽  
Manfred Cejna ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Plasma Cells ◽  
Rapidly Progressive Glomerulonephritis ◽  
Kidney Injury ◽  
Pulmonary Involvement ◽  
Pulmonary Haemorrhage ◽  
Basement Membranes ◽  
High Avidity ◽  
History Of

Abstract Background Anti-glomerular basement membrane disease (GBM) disease is a rare autoimmune disease causing rapidly progressive glomerulonephritis and pulmonary haemorrhage. Recently, an association between COVID-19 and anti-glomerular basement membrane (anti-GBM) disease has been proposed. We report on a patient with recurrence of anti-GBM disease after SARS-CoV-2 infection. Case presentation The 31-year-old woman had a past medical history of anti-GBM disease, first diagnosed 11 years ago, and a first relapse 5 years ago. She was admitted with severe dyspnoea, haemoptysis, pulmonary infiltrates and acute on chronic kidney injury. A SARS-CoV-2 PCR was positive with a high cycle threshold. Anti-GBM autoantibodies were undetectable. A kidney biopsy revealed necrotising crescentic glomerulonephritis with linear deposits of IgG, IgM and C3 along the glomerular basement membrane, confirming a recurrence of anti-GBM disease. She was treated with steroids, plasma exchange and two doses of rituximab. Pulmonary disease resolved, but the patient remained dialysis-dependent. We propose that pulmonary involvement of COVID-19 caused exposure of alveolar basement membranes leading to the production of high avidity autoantibodies by long-lived plasma cells, resulting in severe pulmonary renal syndrome. Conclusion Our case supports the assumption of a possible association between COVID-19 and anti-GBM disease.

In vivo biosynthesis and turnover of glomerular basement membrane in diabetic rats

1982 ◽  
Vol 242 (4) ◽  
pp. F385-F389
Author(s):  
M. P. Cohen ◽  
M. L. Surma ◽  
V. Y. Wu
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Diabetic Rats ◽  
Basement Membranes ◽  
Membrane Turnover ◽  
Proline Incorporation ◽  
Basement Membrane Collagen ◽  
Osmotic Lysis ◽  
Specific Activities

Glomerular basement membrane (GBM) was labeled in vivo by the injection of tracer amounts of tritiated proline into normal and streptozotocin-diabetic rats. Basement membrane biosynthesis and turnover were determined from the specific activities of proline and hydroxyproline in samples purified following osmotic lysis of glomeruli isolated 4 h to 12 days after injection. Peak radiolabeling of normal and diabetic GBM occurred within 24-48 h and 48-72 h, respectively, and, when corrected for differences in the serum proline specific activities, [3H]proline incorporation was greater in diabetic than in normal samples. In contrast to the subsequent time-dependent progressive decline in radiolabeling in basement membranes from normal animals, specific activities of proline and hydroxyproline in diabetic glomerular basement membrane did not change significantly over the same period of observation. Renal cortical mass and glomerular basement membrane collagen content were preserved in diabetic animals despite loss of body weight. The findings are compatible with prolongation of glomerular basement membrane turnover in experimental diabetes, and suggest that diminished degradation contributes to the accumulation of glomerular basement membrane that is characteristic of chronic diabetes.

Losartan preserves glomerular basement membrane anionic charge sites in a rat model of nephropathy

2012 ◽  
Vol 26 (4) ◽  
pp. 660-666 ◽  
Author(s):  
Yan Liu ◽  
Daoyuan Zhou ◽  
Xiao Xiao ◽  
Danping Qin ◽  
Xiaoshi Zhong ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Rat Model ◽  
Glomerular Basement Membrane ◽  
Anionic Charge

Differentiation of glomerular filter and tubular reabsorption apparatus during foetal development of the rat kidney

Development ◽  
1980 ◽  
Vol 58 (1) ◽  
pp. 157-175
Author(s):  
Jean Schaeverbeke ◽  
Madeleine Cheignon
Keyword(s):  
Molecular Weight ◽  
Basement Membrane ◽  
Proximal Tubule ◽  
High Molecular Weight ◽  
Glomerular Basement Membrane ◽  
Rat Kidney ◽  
Tubular Reabsorption ◽  
Foetal Development ◽  
Endocytic Vesicles ◽  
Glomerular Barrier

Differentiation of the glomerulus and the proximal tubule was studied in the rat foetus, especially with regard to the development of the protein filtration-reabsorption apparatus. Filtration starts several days before full differentiation of the glomerulus, when the glomerular basement membrane consists of a thin lamina alongside the podocyte membrane. Endocytosis is functional from this time, but fusion between endocytic vesicles and lysosome-like bodies occurs 2 days later. Foetal urine electrophoresis shows the presence of many proteins, including high molecular weight ones, this proteinuria seeming chiefly due to the immaturity of the glomerular barrier.

scholarly journals Localization and quantitation of anionic charge sites in fetal and neonatal alveolar basement membranes.

1985 ◽  
Vol 33 (6) ◽  
pp. 611-614 ◽  
Author(s):  
T B Ferrara ◽  
S P Sisson ◽  
R L Vernier
Keyword(s):  
Basement Membrane ◽  
Microscopic Analysis ◽  
Basement Membranes ◽  
Lamina Densa ◽  
Human Lung Tissue ◽  
Charge Site ◽  
Electron Microscopic ◽  
Anionic Charge ◽  
Site Localization ◽  
Standard Techniques

A method utilizing biopsy sized samples of lung for anionic charge site localization in alveolar and capillary basement membranes in human tissue is discussed. Tissue fixed in either paraformaldehyde-lysine-periodate or 1% paraformaldehyde with 0.05% glutaraldehyde, cut into 30 mu sections, and incubated with the cationic probe, polyethyleneimine, was processed for electron microscopic analysis using standard techniques. Anionic charge sites were identified and regularly distributed in increments of approximately 40-50 nm in the lamina rara externa of the alveolar basement membrane, with lesser amounts found in the lamina rara interna and lamina densa. Anionic charge sites were also demonstrated in the interstitial portion of the capillary basement membrane and on cell surfaces. These methods can be used to more broadly define the localization of anionic charge sites in human lung tissue in both normal and pathologic states.

Synthesis of the glomerular basement membrane in the rat kidney

1976 ◽  
Vol 20 (1) ◽  
pp. 125-137
Author(s):  
W. Romen ◽  
B. Schultze ◽  
K. Hempel
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Rat Kidney

scholarly journals Analysis of anionic charge of the glomerular basement membrane in aminonucleoside nephrosis

1989 ◽  
Vol 35 (6) ◽  
pp. 1405-1408 ◽  
Author(s):  
Shuzo Kobayashi ◽  
Mitsumasa Nagase ◽  
Nishio Honda ◽  
Kyoko Adachi ◽  
Norio Ichinose ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Anionic Charge ◽  
Aminonucleoside Nephrosis

Correlation between Histological Changes and Loss of Anionic Charge of the Glomerular Basement Membrane in Early-Onset Pre-Eclampsia

Nephron ◽  
1997 ◽  
Vol 75 (2) ◽  
pp. 201-207 ◽  
Author(s):  
T. Naicker ◽  
I.G.H. Randeree ◽  
J. Moodley ◽  
S.M. Khedun ◽  
R. Ramsaroop ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Early Onset ◽  
Histological Changes ◽  
Anionic Charge
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