scholarly journals Preliminary results from a plantation of semi-arid hybrid of Paulownia Clone in vitro 112® under conditions of the Czech Republic from the first two years

2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Jiří Kadlec ◽  
Kateřina Novosadová ◽  
Radek Pokorný
Keyword(s):  
Czech Republic ◽  
Frost Damage ◽  
Continuous Treatment ◽  
Woven Fabric ◽  
The Czech Republic ◽  
Plain Area ◽  
Growing Conditions ◽  
Private Owner ◽  
Semi Arid

A private owner established a plantation of a semi-arid hybrid of Paulownia Clone in vitro 112® near the village of Střelice u Brna in 2016. We split the plantation according to terrain micro-relief (into three expositions: South slope, Plain area and North slope) and according to the applied biotechnology of planting (into two parts: each with planting into 20 and 30 cm holes in diameter). We tested different winter protection techniques for above and belowground plant organs. The results suggest that plants inside the 30 cm holes survive and grow better than those inside the 20 cm ones, regardless of terrain micro-relief. On the other hand, plants inside the 20 cm holes survive and grow better on flat areas compared to the others. The most effective protection of the root system against frost during the wintertime seems to be simple soil covering. We have also recognized that bandage of non-woven fabric is the best protection for the above-ground parts of the plants. However, growing conditions in the Czech Republic (CR) are different to those in semi-arid climate for which researchers bred the Paulownia Clone in vitro 112®. It is possible to achieve well-growing and surviving Paulownia plants under growing conditions of the CR when appropriate biotechnology and continuous treatment are applied. Keywords: Paulownia Clone in vitro 112®, plantation, exposition, mortality, growth, protection against frost damage

scholarly journals Fabian two-row winter barley

2013 ◽  
Vol 49 (No. 4) ◽  
pp. 175-177
Author(s):  
Z. Nesvadba ◽  
J. Špunar ◽  
M. Kadlíková
Keyword(s):  
Czech Republic ◽  
Powdery Mildew ◽  
Plant Height ◽  
Cold Hardiness ◽  
Medium Density ◽  
The Czech Republic ◽  
Variety Trials ◽  
Growing Conditions ◽  
Moderate Resistance ◽  
Very High

Fabian is a medium-late two-row feedin barley of winter habit developed at Agrotest Fyto, Ltd., Kroměříž, Czech Republic (breeders’ rights holder) and registered in the Czech Republic in 2011. In the Official Variety Trials it showed high grain yields under intensive growing conditions (104.5%) and medium yields under low input conditions (101.1%). Fabian has the highest cold hardiness of the registered two-row winter barleys and regenerates very well in the spring. Its resistance to powdery mildew is very high (score 8.2 on a 1–9 scale). It has medium plant height, moderate resistance to lodging and large yellow kernel. The spike is long and of medium density.

scholarly journals Resistance behaviour of Septoria tritici to some fungicides in the territory of the Czech Republic

2012 ◽  
Vol 58 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Ludvik Tvaružek ◽  
P. Horáková ◽  
L. Ji
Keyword(s):  
Czech Republic ◽  
Septoria Tritici ◽  
Resistance Development ◽  
The Czech Republic ◽  
Colony Diameter ◽  
Dmi Fungicides ◽  
The Mean ◽  
Different Parts ◽  
Pathogen Strains

Reduction of colony diameter using two DMI-fungicides and one strobilurine fungicide was evaluated in the <i>Septoria tritici</i> population. In an <i>in vitro</i> assay, 57 strains of <i>S. tritici</i> originating from different parts of the Czech Republic were grown on agar plates containing different concentrations of fungicides. The mean EC<sub>50</sub> values of strains were analysed for flusilazole (Capitain), metconazole (Caramba) and one strobilurine fungicide: azoxystrobin (Amistar). ED 50 values for metconazole were low. There were highly significant correlation in reaction of pathogen strains to metconazole and flusilazole. Some isolates showed significant higher level of resistance to azoxystrobin. The data will be used in a continuing survey of resistance development in the <i>S. tritici</i> population originating from the territory of the Czech Republic.

scholarly journals Analysis of the Frost-Induced Damage of Building Enclosures on the Territory of the Czech Republic

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Jan Kočí ◽  
Jiří Maděra ◽  
Vojtěch Pommer ◽  
Robert Černý
Keyword(s):  
Czech Republic ◽  
Design Process ◽  
Computational Simulation ◽  
Geographic Area ◽  
Frost Damage ◽  
Building Envelope ◽  
Weather Data ◽  
Damage Functions ◽  
The Czech Republic ◽  
Environmental Loads

A comprehensive analysis of environmental loads across the Czech Republic in terms of frost-induced damage is presented. Computational simulation of hygrothermal performance of eleven characteristic types of building envelopes, composed of both contemporary and historical materials, is performed at first. The exterior boundary conditions of the computational model are defined by a set of weather data characterizing the environmental conditions in the Czech Republic, which are acquired from 64 weather stations. The results of hygrothermal simulations are assessed using several specific damage functions. In this way, the basic datasets for the frost damage analysis are obtained. Their application as input parameters of a specially developed correction procedure based on elevation makes them possible to obtain a continuous coverage of the geographic area of the Czech Republic. Finally, isopleths of the supposed frost damage are drawn, depending on the envelope type, and damage maps are produced which may help the engineers to enhance the building envelope design process. The presented results indicate the necessity of paying attention to local environmental loads in the building enclosure design process and reveal both critical and favorable locations from the point of view of frost-induced damage to buildings.

scholarly journals Protocol for efficient micropropagation of spring gentian and sand jurinea

2014 ◽  
Vol 60 (No. 1) ◽  
pp. 1-5
Author(s):  
H. Cvrčková ◽  
P. Máchová ◽  
J. Dostál ◽  
J. Malá
Keyword(s):  
Czech Republic ◽  
Plant Species ◽  
In Vitro Propagation ◽  
Casein Hydrolysate ◽  
Endangered Plant ◽  
Ms Medium ◽  
The Czech Republic ◽  
Endangered Plant Species ◽  
Primary Explants

Protocols for the in vitro propagation of two endangered plant species in the Czech Republic, Gentiana verna L. and Jurinea cyanoides (L.) Rchb., were established. In G. verna, the induction of organogenesis on vegetative shoots was successful on the basal 6% agar WPM medium with 200 mg&middot;l<sup>&ndash;1</sup>of glutamine, 200 mg&middot;l<sup>&ndash;1 </sup>of casein hydrolysate, 30 g&middot;l<sup>&ndash;1 </sup>of sucrose, 0.2 mg&middot;l<sup>&ndash;1 </sup>of BAP, and 0.1 mg&middot;l<sup>&ndash;1 </sup>of IBA. The multiplication of primary explants was achieved on 6% agar basal MS medium with the same concentrations of substances mentioned above. In J. cyanoides, the induction of organogenesis and multiplication of its vegetative shoots were successful on MS medium with the same concentration of added substances used in G. verna. On the basal MS medium of 1/3 concentration with 3 mg&middot;l<sup>&ndash;1 </sup>of IBA 70&ndash;75% rooting efficiency of G. verna microcuttings and 40% rooting efficiency of J. cyanoides microcuttings were reached. The mortality during acclimatization did not exceed 20% for G. verna and 25% for J. cyanoides. &nbsp; &nbsp;

scholarly journals Influence of selected fungicides registered in the Czech Republic for winter oilseed rape on in vitro Sclerotinia sclerotiorum mycelial growth

2018 ◽  
Vol 54 (No. 2) ◽  
pp. 101-110 ◽  
Author(s):  
Poslušná Jana ◽  
Plachká Eva ◽  
Mazáková Jana
Keyword(s):  
Czech Republic ◽  
Oilseed Rape ◽  
Sclerotinia Sclerotiorum ◽  
Mycelial Growth ◽  
Winter Oilseed Rape ◽  
Baseline Sensitivity ◽  
The Czech Republic ◽  
Dose Rates ◽  
The Mean

The baseline sensitivity of 55 isolates of Sclerotinia sclerotiorum, collected from oilseed rape in 6 regions of the Czech Republic, to selected fungicides was determined during the period 2013–2015. One single-component fungicide – Horizon (tebuconazole), and four multicomponent fungicides – Pictor (boscalid, dimoxystrobin), Efilor (boscalid, metconazole), Prosaro 250 EC (prothioconazole, tebuconazole), and Propulse (fluopyram, prothioconazole), were chosen as these are commonly used locally. The effect of each fungicide on the in vitro pathogen radial mycelial growth and EC<sub>50 </sub>values for the respective fungicides were determined. The following MIC values were estimated; for the fungicides Horizon 250 EW, Efilor, and Propulse the mean MIC values ranged between 0.125 and 0.250 µl/ml, for Prosaro 250 EC ranged between 0.0625 and 0.125 µl/ml, and for Pictor ranged from 0.00781 to 0.01562 µl/ml. No strains of S. sclerotiorum resistant to the tested fungicides were detected and the growth of all isolates was fully inhibited at concentrations corresponding to their registered dose rates. The highest fungicidal efficacy on the collected S. sclerotiorum isolates was recorded for Pictor, followed by Prosaro 250 with an EC<sub>50</sub> value 0.05856 µl/ml and then the remaining fungicides Propulse, Efilor, and Horizon 250 EW (EC<sub>50</sub> values 0.07277, 0.07221, and 0.08519 µl/ml, respectively).

scholarly journals First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic

Plant Disease ◽  
2010 ◽  
Vol 94 (8) ◽  
pp. 1071-1071 ◽  
Author(s):  
J. Přibylová ◽  
J. Špak ◽  
D. Kubelková ◽  
K. Petrzik
Keyword(s):  
Czech Republic ◽  
Reverse Transcriptase ◽  
Vaccinium Corymbosum ◽  
Ringspot Virus ◽  
Tween 20 ◽  
Highbush Blueberry ◽  
Reverse Transcriptase Gene ◽  
The Czech Republic ◽  
First Report

A collection of highbush blueberry (Vaccinium corymbosum L.) cultivars planted in the field for propagation in South Bohemia was surveyed in May and July of 2009 for the occurrence of detrimental viruses. A total of 67 plants of 10 cultivars (Berkeley, Burlington, Blue Crop, Bluetta, Darrow, Duke, Gila, Jersey, Late Blue, and Northland), were observed for typical Blueberry red ringspot virus (BRRV) symptoms that appear as reddish ring spots and blotches on stems and fruits, exclusively on the upper surface of the older leaves but not the underside. Samples of leaves were collected and maintained at –20°C until used for DNA extraction, then assayed for BRRV infection using PCR. Controls originated from the same blueberry cultivars in vitro. DNA was extracted from leaf tissue with a NucleoSpin Plant II kit for isolating genomic DNA according to the manufacturer's instructions (Macherey-Nagel, Düren, Germany). Primer pair BRRV15/16, which amplified fragments of the reverse transcriptase gene (1), was used in PCR for BRRV detection. The program used for PCR amplification was 94°C for 2 min, followed by 35 cycles at 94°C for 30 s, 49°C for 30 s, and 70°C for 45 s, followed by a final extension at 70°C for 5 min. The total PCR volume of 25 μl contained 20 ng of DNA, 200 μmol liter–1 dNTPs, 0.5 μl of each primer BRRV15 and BRRV16 (20 pmol μl–1), 75 mM Tris-HCl pH 8.8, 20 mM (NH4)2SO4, 0.01% Tween 20, 2.5 mM MgCl2, 2.5 U of Taq Purple DNA polymerase, and stabilizers (Top-Bio Ltd., Prague, Czech Republic). Amplifications were conducted in an MJ Research (Waltham, MA) thermocycler. Aliquots (4 μl) of each PCR product were analyzed by electrophoresis in tris-acetate-EDTA buffer. No BRRV symptoms were observed on the plants in early spring, yet BRRV was detected in one symptom-free bush of cv. Darrow by PCR. In July, typical symptoms developed on that and another cv. Darrow bush that was also positive by PCR. DNA fragments of the expected sizes were amplified from total nucleic acid samples of both infected blueberry bushes using primers BRRV15/16, while no amplification products were detected in plants without symptoms. The amplicons obtained with primers BRRV15/BRRV16 were sequenced and revealed 97.5%-nt identity to the BRRV putative reverse transcriptase gene (GenBank Accession No. AF404509). The 845 nt of the amplicon has been deposited at GenBank under Accession No. HM107773. The disease was likely introduced in infected planting material, since no highbush blueberry plantations exist in the vicinity and V. corymbosum is not native to the Czech Republic. In conclusion, to our knowledge, this is the first report of Blueberry red ringspot virus (genus Soymovirus, family Caulimoviridae) in V. corymbosum L. in the Czech Republic. Symptom observation and PCR testing for BRRV should therefore, be incorporated into the certification scheme for highbush blueberry in the Czech Republic. Reference: (1) J. J. Polashock et al. Plant Dis. 93:727, 2009.

scholarly journals Damage to corn by fungi of the genus Fusarium and the presence of fusariotoxins

2012 ◽  
Vol 38 (No. 2) ◽  
pp. 46-54 ◽  
Author(s):  
J. Nedělník
Keyword(s):  
Czech Republic ◽  
Fusarium Graminearum ◽  
The Other ◽  
The Czech Republic ◽  
Genus Fusarium ◽  
Toxic Metabolites ◽  
Fungal Contaminants ◽  
Harvest Maturity ◽  
Maximum Content

In 1998 and 1999 a total of 84 samples of corn, predominantly from localities in southern and central Moravia, were collected either directly from fields (entire ears at harvest maturity) or as grain from merchants. The objectives of the experiments were (a) to determine, on the basis of the results from mycological and toxicological analyses, the basic spectrum of fungal contaminants of corn in the Czech Republic with special reference to the genus Fusarium, and (b) to determine by enzyme immunoassay the presence of major toxic metabolites such as deoxynivalenol (DON), T-2 toxin (T-2), zearalenone (ZEA), and fumonisins (FUM) in grain samples. From naturally infected corn, representatives of seven fungal genera were isolated under in vitro conditions in both harvest years. Most frequent were species of the genus Fusarium (mean contamination of 44.6%). The next frequent genus was Stemphylium (29.3%). Eight species of Fusarium were found. In both years the most frequent species was Fusarium graminearum (1998 &ndash; 42.75%, 1999 &ndash; 41.8%), followed by F. culmorum. DON was found in 95.2% of the samples; its content ranged from 25 to 285 &micro;g/kg. The content of T-2 varied more than that of DON, ranging from 12 to 875 &micro;g/kg. Zearalenone content was more varied than that of the trichothecene-type compounds; 17% of the samples did not contain ZEA, the maximum content was 110 &micro;g/kg. No FUM were found in 17% of the samples; in the others, FUM ranged from 12 to nearly 1000 &micro;g/kg. Compared with the other three compounds, fumonisins showed generally the highest levels.

A COMPARATIVE STUDY OF ZYGOTIC TWINNING AND TRIPLET RATES IN EIGHT COUNTRIES, 1972–1999

2003 ◽  
Vol 35 (2) ◽  
pp. 287-302 ◽  
Author(s):  
Y. IMAIZUMI
Keyword(s):  
Czech Republic ◽  
The Netherlands ◽  
Slovak Republic ◽  
Vital Statistics ◽  
The Other ◽  
Federal Republic Of Germany ◽  
The Czech Republic ◽  
England And Wales ◽  
Fertility Drugs

Annual changes in twinning and triplet rates by zygosity were investigated in eight countries during the period 1972–1999 using vital statistics. The monozygotic (MZ) twinning rates in Denmark, Switzerland and the Slovak Republic remained more or less constant throughout this period, whereas those in England and Wales, the Federal Republic of Germany (Germany), the Netherlands, the Czech Republic and Japan increased significantly year by year. With the exception of the Slovak Republic, the dizygotic (DZ) twinning rate increased significantly year by year in each country. It was 2·9 times higher in Denmark and 1·5 times higher in Germany in 1999 than in 1972, and within the same range in the other countries. With two exceptions, the MZ triplet rates remained more or less constant in each country. On the other hand, the DZ and trizygotic (TZ) triplet rates increased significantly year by year in each country. The TZ rate increased 30-fold in Germany, 16·6-fold in Japan, 11·7-fold in Switzerland, 9·7-fold in the Czech Republic, 8·7-fold in the Netherlands, 6·4-fold in Denmark, 5·6-fold in England and Wales and 3·5-fold in the Slovak Republic. The higher DZ twinning rate and higher DZ and TZ triplet rates since 1983 have been attributed to the higher proportion of mothers being treated with ovulation-inducing hormones and in vitro fertilization (IVF) in Denmark, England and Wales, Germany, the Netherlands, Switzerland and Japan. After the introduction of fertility drugs and IVF, variations in the DZ twinning and triplet rates and the TZ triplet rates were not only due to biological factors, but also depended on the popularity of fertility drugs and IVF in each country. In the Slovak Republic, where human fertility might not be affected by some adverse environmental factors, the DZ:MZ ratio remained constant during the period 1972–1999.

scholarly journals Colletotrichum simmondsii Causing Anthracnose on Safflower in the Czech Republic

Plant Disease ◽  
2011 ◽  
Vol 95 (1) ◽  
pp. 79-79 ◽  
Author(s):  
J. Víchová ◽  
K. Vejražka ◽  
T. Cholastová ◽  
R. Pokorný ◽  
E. Hrudová
Keyword(s):  
Czech Republic ◽  
Carthamus Tinctorius ◽  
Colletotrichum Acutatum ◽  
Reaction Products ◽  
Internal Transcribed Spacer Region ◽  
The Czech Republic ◽  
Plant Pathol ◽  
Growing Conditions ◽  
Stem Lesions ◽  
Photoperiod Control

Safflower (Carthamus tinctorius L.) is an oil crop that is suitable for dry growing conditions in the Czech Republic. Most of the Czech production is used as bird feed. Typical anthracnose symptoms were observed at one safflower field in the Moravia Region of the Czech Republic during the 2005 growing season. Since then, the disease has become widespread with 100% yield losses observed in several locations in 2009. Symptoms consisted of circular spots on leaves and stem blight characterized by dark-colored stem lesions bearing salmon-colored conidia masses in acervuli. A fungus was isolated from symptomatic safflower plants (cv. Sabina) on potato dextrose agar and incubated at 25°C as described by Kwon et al. (3). The color of fungal colonies changed from white to gray with age with salmon-orange pigmentation on the reverse side of plates. Similar observations had been reported by Jelev et al. (1). Conidia were colorless, fusiform, and measured 10 to 17 μm (mean 13.59) × 4 to 8 μm (mean 5.98). Morphology suggested a Colletotrichum sp. To fulfill Koch' postulates, safflower plants at the BBCH 12 growth stage (second leaf fully expanded) were spray inoculated with a conidia suspension (1 × 105 conidia/ml). Growth chamber conditions were temperature 20 ± 1°C, relative humidity 70 ± 5%, with a 16-h photoperiod. Control plants were treated with sterile distilled water. Typical anthracnose symptoms were observed 1 week after inoculation. Control plants were symptomless. The pathogen was reisolated from infected stems and leaves. PCR with primers CaInt2 and ITS4 was used to confirm the identification of a Colletotrichum sp. Reaction products obtained with these primers were approximately 500 bp long. The ribosomal DNA internal transcribed spacer region containing ITS1, 5.8S, and ITS2 of the isolate from safflower was sequenced and identified with the BLASTn program. The sequence matches with 100% similarity to the sequence of the Glomerella acutata teleomorph of Colletotrichum acutatum (GenBank Accession No. AB548282) and 100% similarity to C. simmondsii (GenBank Accession No. GU183359). C. acutatum and C. simmondsii can be distinguished from each other by pigment color (4), with the safflower isolate matching the description of C. simmondsii. Kim et al. (2) recorded C. acutatum on safflower fields in the Euiseong area of Korea in 1997. To our knowledge, this is the first report of C. simmondsii causing safflower anthracnose in the Czech Republic. References: (1) Z. J. Jelev et al. Plant Dis. 92:172, 2008. (2) W. G. Kim et al. Plant Pathol. J. 15:62, 1999. (3) J. H. Kwon et al. Plant Pathol. J. 15:172, 1999. (4) R. G. Shivas and Y. P. Tan. Fungal Divers. 39:111, 2009.

scholarly journals Endangerment of thermophilous flora even under conditions of increasing environmental temperatures

2004 ◽  
Vol 52 (2) ◽  
pp. 175-180
Author(s):  
Vladimír Růžička ◽  
Vlasta Ondrová ◽  
Jaroslav Koblížek
Keyword(s):  
Frost Damage ◽  
Rapid Onset ◽  
Climatic Conditions ◽  
Negative Effects ◽  
General Increase ◽  
The Czech Republic ◽  
Air Temperatures ◽  
The Family ◽  
Growing Conditions ◽  
Environmental Temperatures

As mentioned earlier, it is not true that some bulbous species from the family Orchidaceae are able to survive only mycotrophically, i. e. without formation of stalk. Our observations, especially of Ophrys apifera, have demonstrated (in the Czech Republic) that the durability of adult plants is very short so that their numbers are fluctuating. The dying can be caused by several factors. Frost damages followed by rotting of underground parts (roots and bulbs) are relatively frequent. The leaf rosette, which is the most resistant, dies as the last, usually later in the spring of the following year. This means that the frost damage is often not identified during the cursory visually control in the spring. We observated very extensive damaging and dying of the Orchidaceae after the winter of 2002/03 - on the turn of November and December 2002, there was a rapid onset of very strong black frost after a long, wet and relatively mild autumn. Consequently 80% of population perished. None specimens of Ophrys apifera and/or Himantoglossum adriaticum came into blossom in 2003 and other species were strongly damaged. Our observations document that the general increase in air temperatures need not result in the occurrence of generally expected better growing conditions for some thermophilous species. It is very probable that the extremes climatic conditions could show greater effects than the general increase in average temperatures. Such phenomena are well-known but in practice they are not noticed and/or are explained in a different way. Such risks can exist in the whole Central European region. Negative effects of frosts in winter 2002/03 were further intensified by long and extreme droughts in the growing season of the year 2003. Combination of these extremes was crucial for the species Gentianella bohemica: In average, 95% of specimens in each population perished. If the fluctuations in climatic conditions will be more frequent, some species can become extinct irreversibly very quickly.

Sign in / Sign up

Export Citation Format

Share Document