scholarly journals Damage to corn by fungi of the genus Fusarium and the presence of fusariotoxins

2012 ◽  
Vol 38 (No. 2) ◽  
pp. 46-54 ◽  
Author(s):  
J. Nedělník

In 1998 and 1999 a total of 84 samples of corn, predominantly from localities in southern and central Moravia, were collected either directly from fields (entire ears at harvest maturity) or as grain from merchants. The objectives of the experiments were (a) to determine, on the basis of the results from mycological and toxicological analyses, the basic spectrum of fungal contaminants of corn in the Czech Republic with special reference to the genus Fusarium, and (b) to determine by enzyme immunoassay the presence of major toxic metabolites such as deoxynivalenol (DON), T-2 toxin (T-2), zearalenone (ZEA), and fumonisins (FUM) in grain samples. From naturally infected corn, representatives of seven fungal genera were isolated under in vitro conditions in both harvest years. Most frequent were species of the genus Fusarium (mean contamination of 44.6%). The next frequent genus was Stemphylium (29.3%). Eight species of Fusarium were found. In both years the most frequent species was Fusarium graminearum (1998 – 42.75%, 1999 – 41.8%), followed by F. culmorum. DON was found in 95.2% of the samples; its content ranged from 25 to 285 µg/kg. The content of T-2 varied more than that of DON, ranging from 12 to 875 µg/kg. Zearalenone content was more varied than that of the trichothecene-type compounds; 17% of the samples did not contain ZEA, the maximum content was 110 µg/kg. No FUM were found in 17% of the samples; in the others, FUM ranged from 12 to nearly 1000 µg/kg. Compared with the other three compounds, fumonisins showed generally the highest levels.

2003 ◽  
Vol 35 (2) ◽  
pp. 287-302 ◽  
Author(s):  
Y. IMAIZUMI

Annual changes in twinning and triplet rates by zygosity were investigated in eight countries during the period 1972–1999 using vital statistics. The monozygotic (MZ) twinning rates in Denmark, Switzerland and the Slovak Republic remained more or less constant throughout this period, whereas those in England and Wales, the Federal Republic of Germany (Germany), the Netherlands, the Czech Republic and Japan increased significantly year by year. With the exception of the Slovak Republic, the dizygotic (DZ) twinning rate increased significantly year by year in each country. It was 2·9 times higher in Denmark and 1·5 times higher in Germany in 1999 than in 1972, and within the same range in the other countries. With two exceptions, the MZ triplet rates remained more or less constant in each country. On the other hand, the DZ and trizygotic (TZ) triplet rates increased significantly year by year in each country. The TZ rate increased 30-fold in Germany, 16·6-fold in Japan, 11·7-fold in Switzerland, 9·7-fold in the Czech Republic, 8·7-fold in the Netherlands, 6·4-fold in Denmark, 5·6-fold in England and Wales and 3·5-fold in the Slovak Republic. The higher DZ twinning rate and higher DZ and TZ triplet rates since 1983 have been attributed to the higher proportion of mothers being treated with ovulation-inducing hormones and in vitro fertilization (IVF) in Denmark, England and Wales, Germany, the Netherlands, Switzerland and Japan. After the introduction of fertility drugs and IVF, variations in the DZ twinning and triplet rates and the TZ triplet rates were not only due to biological factors, but also depended on the popularity of fertility drugs and IVF in each country. In the Slovak Republic, where human fertility might not be affected by some adverse environmental factors, the DZ:MZ ratio remained constant during the period 1972–1999.


Author(s):  
Jana Niedobová ◽  
Vladimír Hula ◽  
Pavla Šťastná

Collecting of Carabidae was conducted using pitfall traps at four sites. The first two sites (T1 + T2) were at the slope of Macošská stráň and the other two sites (T3 + T4) at the slope of Vilémovická stráň. The study was done in 2008 and 2009. At Macošská stráň in 2008, 21 species of Carabidae with the total number of 228 individuals were found and in 2009, 18 species of the total number of 116 specimens were collected. At Vilémovická stráň in 2008, 22 species of Carabidae with the total number of 1977 specimens were found and in 2009, 21 species of the total number of 623 specimens were caught. In terms of classification of relictness, Macošská stráň in 2008 was dominated by species of adaptable group A (60%), species of eurytop group (E) were represented by 35% and of relic group (R) by 5%. In 2009, the same representation of species of groups A and E (47%) were found and the species of group R were represented by 6%. Vilémovická stráň in 2008 was dominated by species of group A (52%), species of group E were represented by 43% and of group R by 5%. In 2009 also dominated species of group A (54%), species of group E were represented by 41% and of group R by 5%. In the studied area we reported four endangered species of Carabidae protected by Law (No. 395/1992 Coll.) as amended, these were Calosoma auropunctatum (critically endangered), Brachinus crepitans, Carabus ullrichii and Cicindela campestris (endangered) and two species listed under the Red List of Threatened Species of the Czech Republic (Veselý et al., 2005). One of the species is listed as vulnerable (Calosoma auropunctatum) and one as near endangered (Carabus cancellatus). Another significant species found on the monitored sites was Aptinus bombarda.


Author(s):  
Lucie Havlová ◽  
Vladimír Hula ◽  
Jana Niedobová

Araneofauna of vineyards is relatively known in Central Europe but we have a lack of knowledge about araneofauna which occur directly on the vine plants. Our investigation was focused on spiders which live on vine plants, especially on the vine plants trunks. We investigated spiders in six vineyards in southern Moravia (Šatov, Mikulov, Popice, Morkůvky, Nosislav and Blučina). Vineyards were under different soil management, traps were placed on different parts of particular locality (terraced and plain) and all localities were under integrated pest management. We employed two types of cardboard traps for spider collecting during whole vegetation season. Altogether, we collected 21 spider species which belong to seven families. The most important species was Marpissa nivoyi (Lucas, 1836), which is mentioned in the Red List as vulnerable (VU) and Sibianor tantulus (Simon, 1868) which had unknown distribution in the Czech Republic. The other very interesting result is that the most common species is myrmecomorph Synageles venator (Lucas, 1836), which is scarcely recorded in such huge numbers as we documented in our study.


Author(s):  
David Kolumber

The contribution “Anti-state Crimes in Modern Codifications” deals with the topic of anti-state crimes during centuries. Firstly, legal concepts in the pre-state period are mentioned. Then the attention is paid on the evolution of this institute in the Euro-Atlantic area. From this point of view, there are mentioned mainly regulations from England (1351), France (1810), Germany (1871) and Soviet Union (1958) which could be remarked as the most important for the development of other countries. On the other hand, it also reminds the development of codifications in Czechoslovakia, the Czech Republic and Slovakia, which were affected by the Austro-Hungarian legislations as well as German and Soviet approaches. The Czech current arrangement generally does not depart from the other European samples, but it cannot be omitted that in the Czech Republic it is distinguished the penal concept of the high treason (Landesverrat, vlastizrada) from the constitutional concept (Hochverrat, velezrada) which is dedicated to the presidential anti-state activities. The contribution also indicates that the concept of anti-state crimes in countries has not been unified and it has been varying according to various experiences and attitudes.


2013 ◽  
Vol 59 (No. 3) ◽  
pp. 136-142 ◽  
Author(s):  
R. Vácha ◽  
M. Sáňka ◽  
O. Sáňka ◽  
J. Skála ◽  
J. Čechmánková

The upper values of the extractability of trace elements (As, Be, Cd, Co, Cr, Cu, Ni, Pb and Zn) in 2 mol/L HNO<sub>3</sub> and 0.025 mol/L ethylenediaminetetraacetic acid (EDTA) (compared with their pseudototal content in aqua regia) for determination of prevailing anthropogenic and geogenic soil load were proposed and compared with the results of the other 30 Fluvisol samples collected from the Labe fluvial zone. The increased geogenic load of Fluvisols was confirmed in the case of Be and As in some localities where low extractability with increased pseudototal contents were detected as opposed to the other elements when their increased pseudototal contents were followed by their increased extractability. The maps of probability of increased geogenic soil load in the area of the Czech Republic based on the comparison of geological substrates and trace element load were constructed. The combination of proposed elements extractability values for geogenic load together with developed maps is a suitable tool for the definition of prevailing Fluvisol or sediment load on some localities in the whole area of the Czech Republic. The results can be also a useful tool in the decision making processes regarding dredged sediment application on agricultural soil (support tool for legislative norms, Direction No. 257/2009 Sb.).


2011 ◽  
Vol 56 (No. 4) ◽  
pp. 149-155 ◽  
Author(s):  
P. Alexa ◽  
L. Konstantinova ◽  
Z. Sramkova-Zajacova

A survey to estimate the prevalence of verotoxigenic E. coli (VTEC) or enterohaemorrhagic E. coli (EHEC) in rectal swabs from healthy dairy cattle aged three weeks, three months and one year was conducted in three herds from the Czech Republic. Screening for the presence of the stx1, stx2 and eaeA genes in faecal swab cultures was performed by PCR, and in positive samples, isolated colonies were examined. Immunomagnetic separation was used for the isolation of the VTEC serogroup O157 from samples. VTEC were detected in animals from all three herds under study. In the group of 3-week-old calves, VTEC were only detected in samples collected in the summer months. However, in the other age-groups, VTEC were detected in both the summer and winter months. EHEC shedding was observed in 30 to 100% of the total samples collected from cattle aged three months and one year in the summer months, and in 30 to 60% of samples taken in the winter months. EHEC strains of serogroup O157 were detected in two herds. The range of verotoxins shed by VTEC isolates of serogroup O157 differed between herds. Besides serogroup O157, additional EHEC belonging to the antigen groups O26, O103, O128 and O153 have been identified, and in some of them, no somatic antigen was detected.


2019 ◽  
Vol 13 (1) ◽  
pp. 27-44
Author(s):  
Vlasta Škorpíková ◽  
Václav Hlaváč ◽  
Milan Křápek

Abstract In 2015–2016, 6,429 km medium-voltage power lines with 76,430 pylons were checked for bird mortality in the Czech Republic. 1,326 bird victims of power lines were found, 156 of which died after collisions, and 1,170 birds were electrocuted. They belonged to 60 species from 12 orders, and birds of prey made up almost half of all victims. Steel pylons bearing several cross-arms including upper and crosswise jumpers were identified as most dangerous from the electrocution point of view. On the other hand, pylons in straight lines with Pařát cross-arms (triangular arrangement of conductors without any horizontal bar) were among the least dangerous, and when they had a simple perch fitted below the cross-arm, no mortality was recorded. But these pylons are new in practice and despite becoming widely used recently, they form less than one tenth of all pylons in the Czech Republic. On other pylons various types of mitigation measures have been installed. Commonly used plastic covers and plastic strips have proved to be especially effective, but only in cases when they are undamaged and correctly installed.


2008 ◽  
Vol 53 (No. 9) ◽  
pp. 421-431 ◽  
Author(s):  
K. Ryglová

This paper deals with problems of rural tourism development. The works is focused on researching the situation in the area of rural tourism in the Czech Republic, on understanding the attitudes of entrepreneurial sphere and rural population to this entrepreneurship and mainly on the determination of limiting factors that prevent this form of tourism from a more distinctive development. Rural tourism in the Czech Republic is still in the initiating stage of its development and it is not as developed as in some countries in West Europe. This goal has been reached with the help of primary questionnaire inquiry among business and agricultural subjects in rural areas in the individual regions and the detail results of this questionnaire inquiry are enclosed to the following paper. These identified factors were mainly insufficient financial means and at the same time insufficient state assistance connected with this. Insufficient awareness of this support as well as its inaccessibility for small business appears to be a difficulty, too. From the other obstacles, we can mention the current legislation system, bureaucracy, the state of communication and tourist infrastructure, fears of neighbours’ intolerance and losing privacy and also unfamiliarity and little experience.


2000 ◽  
Vol 33 (3) ◽  
pp. 379-402 ◽  
Author(s):  
Eric Hanley

Researchers analyzing self-employment in post-communist Eastern Europe have frequently adopted a “dualist” model which relegates the self-employed to marginal sectors of the economy. This paper challenges the dualist approach and argues that the self-employed cannot be regarded as refugees from poverty with few resources and few opportunities to earn high incomes and accumulate wealth. Data from the Czech Republic, Poland, and Slovakia are used to show that self-employment in post-communist Eastern Europe encompasses two distinct class locations: the individually self-employed on the one hand, whose socioeconomic status differs little from that of ordinary workers, and employers on the other, who receive incomes and possess assets far in excess of that of both the individually self-employed and ordinary workers. A proper understanding of the manner in which systems of stratification have changed in Eastern Europe thus requires that one acknowledge processes of differentiation among the self-employed as well as the importance of property ownership in generating new forms of social inequality in the post-communist period.


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