In vitro activity of methanol extract of Citrullus lanatus Thunb. (Curcubitaceae) fruit rind on isolated gastrointestinal tissues of rabbit and guinea pig

2018 ◽  
Vol 14 (2) ◽  
pp. 244
Author(s):  
Muhajira Ismail ◽  
Bilkisu B. Maiha ◽  
Jamilu Ya’u ◽  
Ibrahim M. Aliyu
Keyword(s):  
Guinea Pig ◽  
Methanol Extract ◽  
Citrullus Lanatus ◽  
Fruit Rind

scholarly journals Triterpenoids, Including One with Smooth Muscle Relaxant Activity, from Rubus idaeus (Raspberry) Leaves

2006 ◽  
Vol 1 (8) ◽  
pp. 1934578X0600100
Author(s):  
Janne Rojas Vera ◽  
Christopher G. Dacke ◽  
Asmita V. Patel ◽  
Gerald Blunden
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Column Chromatography ◽  
Muscle Relaxant ◽  
Methanol Extract ◽  
Rubus Idaeus ◽  
Guinea Pig Ileum ◽  
Muscle Relaxant Activity ◽  
Smooth Muscle Relaxant

A methanol extract of Rubus idaeus leaves was fractionated by column chromatography and the eluted samples were tested for their ability to relax transmurally stimulated Guinea pig ileum preparations in vitro. Two distinct bands of activity were detected. From band one sitosterol and eight triterpenoids were characterized, but only olean-18-ene-1β, 2α, 3α-triol demonstrated smooth muscle relaxant activity. This compound is, to the best of our knowledge, novel, as are olean-12-ene-3β, 29-diol and urs-12-ene-3β, 30-diol.

scholarly journals IN VITRO STUDY OF THE BRONCHORELAXATION EFFECT OF COPTOSAPELTA FLAVESCENS KORTH. ROOT’S EXTRACT AND FRACTIONS

2019 ◽  
pp. 94-97
Author(s):  
KHEMASILI KOSALA ◽  
MOCH. ARIS WIDODO ◽  
SANARTO SANTOSO ◽  
SETYAWATI SOEHARTO
Keyword(s):  
Guinea Pig ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control ◽  
Hexane Fraction ◽  
Organ Bath ◽  
Response Curves ◽  
Methanol Fraction

Objective: This study aimed to compare the bronchorelaxation effect of the methanol extract, hexane fraction, ethyl acetate fraction, and methanol fraction of Coptosapelta flavescens Korth. roots (CFRs) on the isolated bronchial rings of guinea pig. Methods: Research design was experimental in which a 4-mm long bronchus of a male guinea pig was kept in an isolated organ bath containing Krebs-Henseleit solution at 37°C, pH 7.4, and flooded with carbogen gas. The bronchial ring was precontracted with methacholine and then given six doses of C. flavescens Korth. root’s methanol extract (MECFR) solution cumulatively. The extract’s solvent 10% dimethyl sulfoxide ethanol was used as negative control. This procedure was then repeated using CFRs hexane fraction (HFCFR) solution, CFRs ethyl acetate fraction (EAFCFR), and CFRs methanol fraction (MFCFR). Results: The bronchorelaxation effect of MECFR, MFCFR, EAFCFR, and HFCFR is shown by their dose-response curves (DRCs) which are significantly different compared with the extracts solvent’s DRC. The maximal efficacy (Emax) of MFCFR was the same (p>0.05) as the MECFRs Emax, but the EAFCFRs and HFCFRs Emax were smaller (p<0.05) than the MECFRs Emax. Conclusion: The bronchorelaxation effect of the MECFR on the guinea pig’s bronchial ring is similar to the CFRs methanol fraction, and is stronger than the CFRs ethyl acetate fraction and CFRs hexane fraction.

Anti-inflammatory effect of Mitragyna speciosa crude methanol extract on the guinea pig ileum

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
WM Shaik Mossadeq ◽  
K Syamimi ◽  
MP Azyyati ◽  
ZA Zakaria ◽  
AK Arifah ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Methanol Extract ◽  
Guinea Pig Ileum ◽  
Mitragyna Speciosa ◽  
Anti Inflammatory ◽  
Crude Methanol Extract ◽  
Inflammatory Effect

Inhibition of Human and Animal Platelet Adhesiveness to Glass Bead Columns by Adenosine, Dipyridamole, Chlorpromazine and Acetylsalicylic Acid

1976 ◽  
Vol 36 (02) ◽  
pp. 401-410 ◽  
Author(s):  
Buichi Fujttani ◽  
Toshimichi Tsuboi ◽  
Kazuko Takeno ◽  
Kouichi Yoshida ◽  
Masanao Shimizu
Keyword(s):  
Guinea Pig ◽  
Acetylsalicylic Acid ◽  
Guinea Pigs ◽  
Glass Bead ◽  
Animal Species ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Platelet Adhesiveness

SummaryThe differences among human, rabbit and guinea-pig platelet adhesiveness as for inhibitions by adenosine, dipyridamole, chlorpromazine and acetylsalicylic acid are described, and the influence of measurement conditions on platelet adhesiveness is also reported. Platelet adhesiveness of human and animal species decreased with an increase of heparin concentrations and an increase of flow rate of blood passing through a glass bead column. Human and rabbit platelet adhesiveness was inhibited in vitro by adenosine, dipyridamole and chlorpromazine, but not by acetylsalicylic acid. On the other hand, guinea-pig platelet adhesiveness was inhibited by the four drugs including acetylsalicylic acid. In in vivo study, adenosine, dipyridamole and chlorpromazine inhibited platelet adhesiveness in rabbits and guinea-pigs. Acetylsalicylic acid showed the inhibitory effect in guinea-pigs, but not in rabbits.

INHIBITION OF LIPOLYTIC ACTIVITY OF SYNTHETIC β1–24 AND β1–39 CORTICOTROPHIN BY ANTI-ACTH ANTISERUM

1966 ◽  
Vol 51 (1) ◽  
pp. 88-94 ◽  
Author(s):  
A. Villanueva ◽  
S. J. H. Ashcroft ◽  
J. P. Felber
Keyword(s):  
Guinea Pig ◽  
Lipolytic Activity ◽  
Peptide Hormones ◽  
Fat Pad ◽  
Double Antibody ◽  
Epididymal Fat ◽  
Antibody Complex ◽  
Radio Immunoassay ◽  
Antigen Antibody

ABSTRACT The synthetic ACTH peptides β1–39 and β1–24 stimulated lipolysis as determined by the rat epididymal fat pad in vitro. The stimulating effect of these peptides was diminished by prior incubation of the peptides with antibodies produced by the guinea-pig against ACTH. The stimulating effect of these hormones was also diminished by the double antibody system used in the radio-immunoassay of ACTH and other peptide hormones, in which incubation with antiserum is followed by precipitation of the antigen-antibody complex by rabbit anti-guinea-pig-γ-globulin.

The Comparative Study of Equisetum pratense, E. sylvaticum, E. telmateia: Accumulation of Silicon, Antioxidant and Antimicrobial Screening

2019 ◽  
Vol 70 (7) ◽  
pp. 2519-2523
Author(s):  
Denisa Batir Marin ◽  
Oana Cioanca ◽  
Mihai Apostu ◽  
Cristina Gabriela Tuchilus ◽  
Cornelia Mircea ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanol Extract ◽  
Previous Treatment ◽  
Total Phenolic ◽  
Quantitative Chemical Analysis ◽  
Qualitative And Quantitative ◽  
Antioxidant And Antimicrobial Activity ◽  
The Comparative Study

The objective of the current study is represented by the determination of silica and a phytochemical screening of phenolic derivates of some Equisetum species. The antioxidant and antimicrobial activity for Equisetum pratense Ehrh.,, Equisetum sylvaticum L. and Equisetum telmateia Ehrh. (sin. Equisetum maximum Lam.) were also investigated. The concentration of silicon (Si) in plants was determined by the spectrophotometric method using previous treatment with NaOH 50% both for the stem and the nodal branches [1]. Results obtained varied from 95.12 to 162.10 SiO2 mg/g dry plant which represents 4.44% to 7.58% Si/100g dry sample. Two types of total extracts were obtained using different solvents and were subjected to qualitative and quantitative chemical analysis considering total phenolic content [2]. The highest concentration of investigated compounds was found in the methanolic extract, E. sylvaticum, 196.5mg/g dry sample. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (�g/mL) [3]. Values gathered ranged from 261.7 to 429.5 �g/mL. The highest capacity to neutralized DPPH radicals was found in E. sylvaticum. In vitro antimicrobial activity was determined using difusimetric method [4]. Testing was performed on four microorganisms: three strains of bacteria and one species of fungi. Different effects were noticed against the bacteria, furthermore the methanol extract appeared to be most efficient. All extracts showed significand antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Candida albicans (ATCC 90028) and weak to no activity against Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922).

Sign in / Sign up

Export Citation Format

Share Document