GLIADIN COMPOSITION OF THE BREAD-WHEAT CULTIVARS BW 20 AND SINTON

1979 ◽  
Vol 59 (4) ◽  
pp. 1001-1005 ◽  
Author(s):  
F. G. KOSMOLAK
Keyword(s):  
Bread Wheat ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Test Plot ◽  
Wheat Cultivars ◽  
Electrophoretic Patterns ◽  
Yield Test

Gliadins from 123 breeder lines of the cultivar BW 20 and from 198 breeder lines of the cultivar Sinton were extracted and separated by polyacrylamide gel electrophoresis. The cultivar BW 20 consisted of four biotypes and the cultivar Sinton of five with respect to their gliadin composition. The major biotype was represented by 93% of the breeder lines of the cultivar BW 20 and by 98% of the cultivar Sinton. The electrophoretic patterns of awned off-type plants in a yield test plot of Cultivar BW 20 were different from the patterns of the biotypes present in breeder lines of BW 20. It was concluded that these awned plants were contaminants from other cultivar sources.

IDENTIFICATION OF CULTIVARS OF FORAGE LEGUME (Desmodium ovalifolium GUILL ET PERR.) BY THEIR ELECTROPHORETIC PATTERNS

1987 ◽  
Vol 67 (3) ◽  
pp. 713-717 ◽  
Author(s):  
A. HUSSAIN ◽  
W. BUSHUK ◽  
H. RAMIREZ ◽  
W. ROCA
Keyword(s):  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel ◽  
Forage Legume ◽  
Desmodium Ovalifolium ◽  
Electrophoretic Patterns ◽  
Electrophoretic Procedure

An electrophoretic procedure was developed for discriminating cultivars of Desmodium ovalifolium on the basis of patterns of partially purified seed proteins. Electrophoresis was done on uniform 15% polycrylamide gels in basic (8.9) pH. The method produced satisfactory discrimination of eight cultivars used in its initial evaluation.Key words: Forage legume, Desmodium ovalifolium Guill et Perr., cultivar identification, polyacrylamide gel electrophoresis

scholarly journals Polymorphism of Gli-D1 alleles of Kragujevac’s winter wheat cultivars (Triticum aestivum L.)

Genetika ◽  
2007 ◽  
Vol 39 (2) ◽  
pp. 273-282
Author(s):  
Desimir Knezevic ◽  
Aleksandra Novoselskaya-Dragovich
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Molecular Mass ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Triticum Aestivum L ◽  
Polyacrylamide Gel ◽  
Wheat Cultivars ◽  
Number Of Components ◽  
Winter Wheat Cultivars

Composition of gliadins encoded by Gli-D1 allele as well polymorphisms of Gli-D1 allele investigated in 25 wheat cultivars by using acid polyacrylamide gel electrophoresis. Electrophoregrams obtained by polyacrylamide gel electrophoresis were used for estimation variability of gliadin components and identification of gliadin blocks. Five gliadin blocks encoded by different alleles at Gli-D1 locus were apparently expressed and identified. Gliadin blocks differed according to number of components and their molecular mass. Variability of determined block components indicates that existing polymorphisms of gliadins alleles. Frequency of identified 5 alleles at Gli-D1 locus was in ratio from 4% to 52%. The highest frequency of b allele and the of g allele was found.

scholarly journals Similarity of cultivars of wheat (Triticum durum) on the basis of composition of Gliadin alleles

Genetika ◽  
2011 ◽  
Vol 43 (3) ◽  
pp. 527-536
Author(s):  
Nevena Djukic ◽  
Desimir Knezevic ◽  
Daniela Horvat ◽  
Dragan Zivancev ◽  
Aleksandra Torbica
Keyword(s):  
Durum Wheat ◽  
Gel Electrophoresis ◽  
Triticum Durum ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Wheat Cultivars ◽  
Allele Composition ◽  
Gliadin Alleles

Twenty one durum wheat cultivars originating from different world countries were investigated. Composition of gliadins was analyzed by acid polyacrylamide gel electrophoresis. Allele composition of gliadins was determined on the basis of identified gliadin blocks. Polymorphisms of Gli- loci was established and 27 different gliadin alleles were identified, namely, 5 at Gli-A1, 4 at Gli-B1, 9 at Gli-A2 and 9 alleles at Gli-B2 locus. The catalogue of determined alleles was presented. Frequency of alleles ranged from 4.76% to 42.86%. Heterozygous Gli-loci were identified at two durum cultivars. Similarity among cultivars was studied on composition of Gli-alleles and presented by UPGMA dendogram. On the base of Gli-allele composition, similarity varied from 0% to 100%.

Polyacrylamide gel electrophoresis and infrared spectroscopy of vibrio biotypes

1981 ◽  
Vol 27 (10) ◽  
pp. 1048-1052 ◽  
Author(s):  
M. Maiti ◽  
P. Sur ◽  
S. N. Chatterjee
Keyword(s):  
Infrared Spectroscopy ◽  
Vibrio Cholerae ◽  
Infrared Spectra ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Electrophoretic Patterns ◽  
Different Strains ◽  
El Tor

Polyacrylamide gel electrophoresis and infrared spectroscopy were used to study the relationship between Vibrio cholerae (classical), Vibrio cholerae (El Tor), and nonagglutinable (NAG) vibrios. Acid extracts of the different strains produced type-specific electrophoretic patterns, and the infrared spectra revealed broad absorption maxima which largely correspond to those found in other organisms. With the exception of the NAG vibrios, the infrared spectra of cholera El Tor vibrios were identical. Strain-specific differences were found in the exoprotein spectra of these organisms by the sodium dodecyl sulphate – polyacrylamide gel electrophoretic technique.

Gliadin alleles in Canadian western red spring wheat cultivars: use of two different procedures of acid polyacrylamide gel electrophoresis for gliadin separation

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 743-749 ◽  
Author(s):  
E. V. Metakovsky ◽  
P. K. W. Ng ◽  
V. M. Chernakov ◽  
N. E. Pogna ◽  
W. Bushuk
Keyword(s):  
Common Wheat ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Component Composition ◽  
Polyacrylamide Gel ◽  
Marker Genes ◽  
Wheat Cultivars ◽  
Gliadin Alleles ◽  
Gliadin Allele ◽  
Spring Common Wheat

Gliadin allele compositions of 21 Canadian spring common wheat cultivars, most of which belong to the Canada western red spring (CWRS) class, were studied and great similarity in their genotypes was confirmed. It was found that alleles frequent in the set of Canadian wheats (such as Gli-B1d, Gli-D1j, Gli-A2m, and Gli-D2h) are very rare or absent in common wheat cultivars from other regions and countries studied earlier, indicating that germplasm of CWRS cultivars is rather unique. It may be suggested that alleles frequent in Canadian cultivars relate to important technological characteristics of these wheats and may possibly serve as marker genes during selection for quality traits. Similarity of gliadin electrophoregrams obtained by two different acid polyacryl-amide gel electrophoretic procedures for the same genotype was established, and the component composition of allelic variants of blocks of gliadin components found in the set of Canadian cultivars and in standard cultivars Chinese Spring and Bezostaya 1 are described.Key words: gliadin alleles, acid polyacrylamide gel electrophoresis, Canadian bread wheats.

scholarly journals Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins

2015 ◽  
Vol 48 (4) ◽  
pp. 511-521
Author(s):  
Aleksander F. Sikorski
Keyword(s):  
Membrane Proteins ◽  
Chlamydomonas Reinhardtii ◽  
Outer Surface ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Electrophoretic Analysis ◽  
Polyacrylamide Gel ◽  
Electrophoretic Patterns

SDS-polyacrylamide electrophoretic patterns of <i>Chlamydomonas</i> flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and internal localization of fractions '4' and '6'. Application of SDS-polyacrylamide gel electrophoresis and radiolabeled membranes allowed to isolate individual membrane polypeptides.

Isoenzymatic pattern of glucose 6-phosphate dehydrogenase from Ascaris suum

1993 ◽  
Vol 67 (3) ◽  
pp. 226-232 ◽  
Author(s):  
C. Cutillas ◽  
B. Rodriguez ◽  
P. German ◽  
D. Guevara
Keyword(s):  
Gel Electrophoresis ◽  
Ascaris Suum ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Starch Gel Electrophoresis ◽  
Specific Staining ◽  
Electrophoretic Patterns ◽  
Starch Gel ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Isoenzymatic Pattern

AbstractThe isoenzymatic pattern of glucose 6-phosphate dehydrogenase (abbreviation G6PD) from Ascaris suum has been studied by vertical polyacrylamide gel (PAGE) and horizontal starch gel electrophoresis. After polyacrylamide gel electrophoresis, two stained zones could be identified. One corresponded to tetrazolium oxidase activity; since this zone was stained even in the absence of glucose 6-phosphate (G6P), non-specific staining could be detected. In the other zone of activity, seven regularly-spaced bands were identified by staining in the presence of G6P and NADP as substrates. By using starch gel electrophoresis, different electrophoretic patterns for G6PD have been observed in the muscular sac, intestine and reproductive system from A. suum. The existence of three different alleles of G6PD in the same individual suggests the existence of at least two genes for this enzyme.

Action of milk-clotting enzymes on β-caseins from buffalo's and cow's milk

1976 ◽  
Vol 43 (3) ◽  
pp. 443-448 ◽  
Author(s):  
Safinaz El-Shibiny ◽  
M. H. Abd El-Salam
Keyword(s):  
Gel Electrophoresis ◽  
Degradation Products ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Cow’S Milk ◽  
Mucor Miehei ◽  
Cow's Milk ◽  
Milk Clotting ◽  
Electrophoretic Patterns ◽  
Endothia Parasitica

Summaryβ-Caseins isolated from buffalo's and cow's milk were hydrolysed either with rennet or with microbial proteases fromMucor miehei, M. pusillusLindt orEndothia parasitica. The degradation products were separated by polyacrylamide-gel electrophoresis and the residual β-casein was determined quantitatively after various times. The electrophoretic patterns of the degradation products of buffalo and bovine β-casein produced by the different enzymes were not identical. β-Casein of buffalo's milk was hydrolysed by rennet andM. mieheiprotease at a slower rate than that of cow's milk. The reverse was found withE. parasiticaandM. pusillusLindt proteases. Carbamylation of buffalo β-casein was found to retard its proteolysis by all the enzymes but particularly by rennet andM. mieheiprotease.

The electrophoretic patterns of proteins extracted from spores and mycelium of two Drechslera species

1970 ◽  
Vol 48 (5) ◽  
pp. 1000-1002 ◽  
Author(s):  
W. A. Shipton ◽  
W. C. McDonald
Keyword(s):  
Present State ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Fungal Taxonomy ◽  
Polyacrylamide Gels ◽  
Interspecies Differences ◽  
Electrophoretic Patterns ◽  
Mycelial Extract ◽  
Electrophoresis Of Proteins

Electrophoresis of protein extracts from spores and mycelium of two Drechslera species (D. erythrospilum and D. teres) was carried out on polyacrylamide gels. Spores were harvested from V-8 juice agar and mycelium from V-8 juice broth cultures. One or two band differences were found between electrophoretic patterns of spore extract and mycelial extract depending on the species. Interspecies differences in patterns were no greater than intraspecies differences. The results are discussed, and it is suggested that the method of polyacrylamide gel electrophoresis of proteins is unsuitable as an aid to fungal taxonomy in its present state of development.

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