Isoenzymatic pattern of glucose 6-phosphate dehydrogenase from Ascaris suum

The isoenzymatic pattern of glucose 6-phosphate dehydrogenase (abbreviation G6PD) from Ascaris suum has been studied by vertical polyacrylamide gel (PAGE) and horizontal starch gel electrophoresis. After polyacrylamide gel electrophoresis, two stained zones could be identified. One corresponded to tetrazolium oxidase activity; since this zone was stained even in the absence of glucose 6-phosphate (G6P), non-specific staining could be detected. In the other zone of activity, seven regularly-spaced bands were identified by staining in the presence of G6P and NADP as substrates. By using starch gel electrophoresis, different electrophoretic patterns for G6PD have been observed in the muscular sac, intestine and reproductive system from A. suum. The existence of three different alleles of G6PD in the same individual suggests the existence of at least two genes for this enzyme.

Isoenzymatic pattern and structure of glutamate dehydrogenase from Ascaris suum

ABSTRACTThe isoenzymatic pattern of glutamate dehydrogenase (GDH) has been described for Ascaris suum, a parasite of Sus scrofa domestica. Only one band of activity has been revealed, suggesting a monomorphic condition for this enzyme. Also, the structure of GDH has been assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) and silver staining. Only one subunit was present with a molecular weight of about 55 000. A hexameric structure for GDH of A. suum is suggested.

Glucose 6-phosphate dehydrogenase: isoenzymatic pattern in Oesophagostomum venulosum, Trichuris ovis and T. suis

ABSTRACTIn the present communication we have studied the isoenzymatic pattern activity of the glucose 6-phosphate dehydrogenase (G6PD) in Oesophagostomum venulosum, Trichuris ovis and T. suis, parasites of Capra hircus (goat), Ovis aries (sheep) and Sus scrofa domestica (pig) respectively, by polyacrylamide gel electrophoresis. Different phenotypes have been observed in the G6PD isoenzymatic pattern activity in males and females of Oesophagostomum venulosum. Furthermore, G6PD activity has been assayed in Trichuris ovis collected from Ovis aries and Capra hircus. No differences have been observed in the isoenzymatic patterns attending to the different hosts. All the individuals exhibited one single band or two bands; this suggests a monomeric condition for G6PD in T. ovis. In T. suis the enzyme G6PD appeared as a single electrophoretic band in about 85·7% of the individuals.

In vitro genetic variability of sugarcane cultivars

Genotypic stability and uniformity of field plants from sugarcane cultivars grown in vitro (calli and cell suspensions) were evaluated by isoenzymatic peroxidase analysis after regeneration, Phenotypes of field plants exhibited uniformity within cultivars and differences among cultivars. Distribution of PRX isoenzymes was different in the constitution of the calli phenotypes, not only among cultivars but also within the same cultivar. Genotype PR980 showed a 60% heterogeneity coefficient, whereas the call of cultivars PR62258, PR641791, PR692176, V64-10 and V71-51 showed identity coefficient of 100%. The isoenzymatic pattern of PRX for the phenotypes of cell suspensions was different among the four cuitivars used, as well as within cultivars PR980 and V64-10, all of which showed heterogeneity coefficients of 50 and 22%, respectively. Nevertheless, the identity coefficient for regenerated plants of all genotypes was 100%. In sugarcane, the isoenzyme PRX1 is related to differentiated tissue, since it is present only in phenotypes from field and regenerated plants. PRX4 is induced in vitro conditions since it was observed only in callus tissue and ceil suspensions,This study detected high uniformity and genetic stability within the regenerated plants in 100% of the sugarcane cultivars used.