Gliadin alleles in Canadian western red spring wheat cultivars: use of two different procedures of acid polyacrylamide gel electrophoresis for gliadin separation

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 743-749 ◽  
Author(s):  
E. V. Metakovsky ◽  
P. K. W. Ng ◽  
V. M. Chernakov ◽  
N. E. Pogna ◽  
W. Bushuk
Keyword(s):  
Common Wheat ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Component Composition ◽  
Polyacrylamide Gel ◽  
Marker Genes ◽  
Wheat Cultivars ◽  
Gliadin Alleles ◽  
Gliadin Allele ◽  
Spring Common Wheat

Gliadin allele compositions of 21 Canadian spring common wheat cultivars, most of which belong to the Canada western red spring (CWRS) class, were studied and great similarity in their genotypes was confirmed. It was found that alleles frequent in the set of Canadian wheats (such as Gli-B1d, Gli-D1j, Gli-A2m, and Gli-D2h) are very rare or absent in common wheat cultivars from other regions and countries studied earlier, indicating that germplasm of CWRS cultivars is rather unique. It may be suggested that alleles frequent in Canadian cultivars relate to important technological characteristics of these wheats and may possibly serve as marker genes during selection for quality traits. Similarity of gliadin electrophoregrams obtained by two different acid polyacryl-amide gel electrophoretic procedures for the same genotype was established, and the component composition of allelic variants of blocks of gliadin components found in the set of Canadian cultivars and in standard cultivars Chinese Spring and Bezostaya 1 are described.Key words: gliadin alleles, acid polyacrylamide gel electrophoresis, Canadian bread wheats.

scholarly journals Similarity of cultivars of wheat (Triticum durum) on the basis of composition of Gliadin alleles

Genetika ◽  
2011 ◽  
Vol 43 (3) ◽  
pp. 527-536
Author(s):  
Nevena Djukic ◽  
Desimir Knezevic ◽  
Daniela Horvat ◽  
Dragan Zivancev ◽  
Aleksandra Torbica
Keyword(s):  
Durum Wheat ◽  
Gel Electrophoresis ◽  
Triticum Durum ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Wheat Cultivars ◽  
Allele Composition ◽  
Gliadin Alleles

Twenty one durum wheat cultivars originating from different world countries were investigated. Composition of gliadins was analyzed by acid polyacrylamide gel electrophoresis. Allele composition of gliadins was determined on the basis of identified gliadin blocks. Polymorphisms of Gli- loci was established and 27 different gliadin alleles were identified, namely, 5 at Gli-A1, 4 at Gli-B1, 9 at Gli-A2 and 9 alleles at Gli-B2 locus. The catalogue of determined alleles was presented. Frequency of alleles ranged from 4.76% to 42.86%. Heterozygous Gli-loci were identified at two durum cultivars. Similarity among cultivars was studied on composition of Gli-alleles and presented by UPGMA dendogram. On the base of Gli-allele composition, similarity varied from 0% to 100%.

scholarly journals Polymorphism of Gli-D1 alleles of Kragujevac’s winter wheat cultivars (Triticum aestivum L.)

Genetika ◽  
2007 ◽  
Vol 39 (2) ◽  
pp. 273-282
Author(s):  
Desimir Knezevic ◽  
Aleksandra Novoselskaya-Dragovich
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Molecular Mass ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Triticum Aestivum L ◽  
Polyacrylamide Gel ◽  
Wheat Cultivars ◽  
Number Of Components ◽  
Winter Wheat Cultivars

Composition of gliadins encoded by Gli-D1 allele as well polymorphisms of Gli-D1 allele investigated in 25 wheat cultivars by using acid polyacrylamide gel electrophoresis. Electrophoregrams obtained by polyacrylamide gel electrophoresis were used for estimation variability of gliadin components and identification of gliadin blocks. Five gliadin blocks encoded by different alleles at Gli-D1 locus were apparently expressed and identified. Gliadin blocks differed according to number of components and their molecular mass. Variability of determined block components indicates that existing polymorphisms of gliadins alleles. Frequency of identified 5 alleles at Gli-D1 locus was in ratio from 4% to 52%. The highest frequency of b allele and the of g allele was found.

Diversity of seed storage proteins in common wheat (Triticum aestivum L.)

2011 ◽  
Vol 9 (2) ◽  
pp. 256-259
Author(s):  
Zuzana Šramková ◽  
Edita Gregová ◽  
Svetlana Šliková ◽  
Ernest Šturdík
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Gel Electrophoresis ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L ◽  
Polyacrylamide Gel

The objective of our study was to determine the composition of high-molecular weight-glutenin subunits (HMW-GS) in 120 cultivars of common wheat (Triticum aestivum L.). Fourteen alleles and 34 allelic compositions were detected using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1 and Glu-D1 loci were null (57.1%), 7+9 (43.3%) and 5+10 (61.9%), respectively. However, low-frequency HMW-GS alleles were also observed, such as 13+16, 20, 21, 7 and 18, encoded by the Glu-B1 locus, and 4+12, encoded by the Glu-D1 locus. The wheat–rye 1BL.1RS translocation was identified in 25 cultivars, using acid polyacrylamide gel electrophoresis. The Glu-score varied greatly, and some lines reached the maximum value of 10.

GLIADIN COMPOSITION OF THE BREAD-WHEAT CULTIVARS BW 20 AND SINTON

1979 ◽  
Vol 59 (4) ◽  
pp. 1001-1005 ◽  
Author(s):  
F. G. KOSMOLAK
Keyword(s):  
Bread Wheat ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Test Plot ◽  
Wheat Cultivars ◽  
Electrophoretic Patterns ◽  
Yield Test

Gliadins from 123 breeder lines of the cultivar BW 20 and from 198 breeder lines of the cultivar Sinton were extracted and separated by polyacrylamide gel electrophoresis. The cultivar BW 20 consisted of four biotypes and the cultivar Sinton of five with respect to their gliadin composition. The major biotype was represented by 93% of the breeder lines of the cultivar BW 20 and by 98% of the cultivar Sinton. The electrophoretic patterns of awned off-type plants in a yield test plot of Cultivar BW 20 were different from the patterns of the biotypes present in breeder lines of BW 20. It was concluded that these awned plants were contaminants from other cultivar sources.

ASSOCIATION BETWEEN GLIADIN ELECTROPHORETIC BANDS AND QUALITY IN COMMON WHEAT

1982 ◽  
Vol 62 (4) ◽  
pp. 913-918 ◽  
Author(s):  
N. E. POGNA ◽  
G. BOGGINI ◽  
M. CORBELLINI ◽  
M. CATTANEO ◽  
A. DAL BELIN PERUFFO
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Triticum Aestivum L ◽  
Loaf Volume ◽  
Wheat Cultivars ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Twenty common wheat (Triticum aestivum L.) cultivars were classified into two groups based on the presence or absence in their electrophoregrams of two gliadin bands separated by polyacrylamide gel electrophoresis. The two groups were compared for quality and agronomic characteristics evaluated during 6 yr of testing. The group 1 cultivars (band 40 present, 43.5 absent) had weaker gluten properties and lower loaf volume than cultivars in group 2 (band 40 absent, 43.5 present). No significant difference between the groups was found for protein content or grain yield. The present data are discussed in relation to similar results obtained by other authors for durum wheat cultivars.

Ultrastructural localization of specific nucleoprotein fractions

1978 ◽  
Vol 36 (2) ◽  
pp. 204-205
Author(s):  
G. L. Brown
Keyword(s):  
Gel Electrophoresis ◽  
Mouse Liver ◽  
Polyacrylamide Gel Electrophoresis ◽  
Ultrastructural Localization ◽  
Polyacrylamide Gel ◽  
Acid Extraction ◽  
Acid Solutions ◽  
Low Salt ◽  
Liver Nuclei ◽  
Phosphate Groups

Bismuth (Bi) stains nucleoproteins (NPs) by interacting with available amino and primary phosphate groups. These two staining mechanisms are distinguishable by glutaraldehyde crosslinking (Fig. 1,2).Isolated mouse liver nuclei, extracted with salt and acid solutions, fixed in either formaldehyde (form.) or gl utaraldehyde (glut.) and stained with Bi, were viewed to determine the effect of the extractions on Bi stainina. Solubilized NPs were analyzed by SDS-polyacrylamide gel electrophoresis.Extraction with 0.14 M salt does not change the Bi staining characteristics (Fig. 3). 0.34 M salt reduces nucleolar (Nu) staining but has no effect on interchromatinic (IC) staining (Fig. 4). Proteins responsible for Nu and glut.- insensitive IC staining are removed when nuclei are extracted with 0.6 M salt (Fig. 5, 6). Low salt and acid extraction prevents Bi-Nu staining but has no effect on IC staining (Fig. 7). When nuclei are extracted with 0.6 M salt followed by low salt and acid, all Bi-staining components are removed (Fig. 8).

Platelet Microtubule Subunit Proteins

1979 ◽  
Vol 42 (05) ◽  
pp. 1630-1633 ◽  
Author(s):  
A G Castle ◽  
N Crawford
Keyword(s):  
Epithelial Cells ◽  
Gel Electrophoresis ◽  
Blood Platelets ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Lens Epithelial Cells ◽  
Molecular Weights ◽  
Kinetics Of ◽  
Microtubular Structures

SummaryBlood platelets contain microtubule proteins (tubulin and HMWs) which can be polymerised “in vitro” to form structures which resemble the microtubules seen in the intact platelet. Platelet tubulin is composed of two non-identical subunits a and p tubulin which have molecular weights around 55,000 but can be resolved in alkaline SDS-polyacrylamide gel electrophoresis. These subunits associate as dimers with sedimentation coefficients of about 5.7 S although it is not known whether the dimer protein is a homo- or hetero-dimer. The dimer tubulin binds the anti-mitotic drug colchicine and the kinetics of this binding are similar to those reported for neurotubulins. Platelet microtubules also contain two HMW proteins which appear to be essential and integral components of the fully assembled microtubule. These proteins have molecular weights greater than 200,000 daltons. Fluorescent labelled antibodies to platelet and brain tubulins stain long filamentous microtubular structures in bovine lens epithelial cells and this pattern of staining is prevented by exposing the cells to conditions known to cause depolymerisation of cell microtubules.

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