The electrophoretic patterns of proteins extracted from spores and mycelium of two Drechslera species

1970 ◽  
Vol 48 (5) ◽  
pp. 1000-1002 ◽  
Author(s):  
W. A. Shipton ◽  
W. C. McDonald
Keyword(s):  
Present State ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Fungal Taxonomy ◽  
Polyacrylamide Gels ◽  
Interspecies Differences ◽  
Electrophoretic Patterns ◽  
Mycelial Extract ◽  
Electrophoresis Of Proteins

Electrophoresis of protein extracts from spores and mycelium of two Drechslera species (D. erythrospilum and D. teres) was carried out on polyacrylamide gels. Spores were harvested from V-8 juice agar and mycelium from V-8 juice broth cultures. One or two band differences were found between electrophoretic patterns of spore extract and mycelial extract depending on the species. Interspecies differences in patterns were no greater than intraspecies differences. The results are discussed, and it is suggested that the method of polyacrylamide gel electrophoresis of proteins is unsuitable as an aid to fungal taxonomy in its present state of development.

IDENTIFICATION OF CULTIVARS OF FORAGE LEGUME (Desmodium ovalifolium GUILL ET PERR.) BY THEIR ELECTROPHORETIC PATTERNS

1987 ◽  
Vol 67 (3) ◽  
pp. 713-717 ◽  
Author(s):  
A. HUSSAIN ◽  
W. BUSHUK ◽  
H. RAMIREZ ◽  
W. ROCA
Keyword(s):  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel ◽  
Forage Legume ◽  
Desmodium Ovalifolium ◽  
Electrophoretic Patterns ◽  
Electrophoretic Procedure

An electrophoretic procedure was developed for discriminating cultivars of Desmodium ovalifolium on the basis of patterns of partially purified seed proteins. Electrophoresis was done on uniform 15% polycrylamide gels in basic (8.9) pH. The method produced satisfactory discrimination of eight cultivars used in its initial evaluation.Key words: Forage legume, Desmodium ovalifolium Guill et Perr., cultivar identification, polyacrylamide gel electrophoresis

Polyacrylamide gel electrophoresis and infrared spectroscopy of vibrio biotypes

1981 ◽  
Vol 27 (10) ◽  
pp. 1048-1052 ◽  
Author(s):  
M. Maiti ◽  
P. Sur ◽  
S. N. Chatterjee
Keyword(s):  
Infrared Spectroscopy ◽  
Vibrio Cholerae ◽  
Infrared Spectra ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Electrophoretic Patterns ◽  
Different Strains ◽  
El Tor

Polyacrylamide gel electrophoresis and infrared spectroscopy were used to study the relationship between Vibrio cholerae (classical), Vibrio cholerae (El Tor), and nonagglutinable (NAG) vibrios. Acid extracts of the different strains produced type-specific electrophoretic patterns, and the infrared spectra revealed broad absorption maxima which largely correspond to those found in other organisms. With the exception of the NAG vibrios, the infrared spectra of cholera El Tor vibrios were identical. Strain-specific differences were found in the exoprotein spectra of these organisms by the sodium dodecyl sulphate – polyacrylamide gel electrophoretic technique.

An analysis of the proteinases ofTrichomonas vaginalisby polyacrylamide gel electrophoresis

Parasitology ◽  
1983 ◽  
Vol 86 (1) ◽  
pp. 1-6 ◽  
Author(s):  
G. H. Coombs ◽  
M. J. North
Keyword(s):  
Gel Electrophoresis ◽  
Trichomonas Vaginalis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Cysteine Proteinases ◽  
Polyacrylamide Gels

SUMMARYThe proteinases ofTrichomonas vaginalishave been analysed by electrophoresis in polyacrylamide gels containing denatured haemoglobin. Seven bands of activity were detected indicating multiple proteinases. All of the enzymes were stimulated by 1 mM dithiothreitol and had inhibitor sensitivities characteristic of cysteine proteinases. The enzymes differed significantly, however, with respect to pH optima and relative sensitivities to inhibitors.

A COMPARATIVE STUDY OF PREPARATIONS OF OVINE LUTEINIZING HORMONE BY BIOASSAY, IMMUNODOUBLEDIFFUSION, RADIOIMMUNOASSAY, RADIORECEPTOR ASSAY AND POLYACRYLAMIDE GEL ELECTROPHORESIS

1976 ◽  
Vol 81 (2) ◽  
pp. 270-282 ◽  
Author(s):  
M. Schlamowitz ◽  
J. Cronquist ◽  
M. Esfahani ◽  
D. N. Ward
Keyword(s):  
Luteinizing Hormone ◽  
Comparative Study ◽  
Gel Electrophoresis ◽  
Binding Capacity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Radioreceptor Assay ◽  
Polyacrylamide Gel ◽  
Polyacrylamide Gels ◽  
Biological Potency

ABSTRACT Three preparations of ovine LH were compared for biological potency and by several in vitro parameters. All were found to be heterogenous by immunodoublediffusion and by electrophoresis in polyacrylamide gels. They all also showed similarities and/or differences with respect to their characteristics in immunodoublediffusion, radioimmunoassay, radioreceptor assay, gel electrophoresis and in dye-binding capacity, but in ways that preclude establishing a meaningful correlation between biopotency and the in vitro parameters or even among the in vitro parameters themselves. The implications of these findings for the use of these in vitro parameters for screening and assessing biological potencies of LH preparations and for inferring chemical and/or structural similarities between LH preparations are discussed. Aspects of polymorphism of LH, observed by electrophoresis on polyacrylamide gels, are also discussed.

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