BARLEY CULTIVAR IDENTIFICATION BY USE OF ISOZYME ELECTROPHORETIC PATTERNS

1976 ◽  
Vol 56 (1) ◽  
pp. 1-6 ◽  
Author(s):  
ABDOLLAH BASSIRI
Keyword(s):  
Acid Phosphatase ◽  
Hordeum Vulgare ◽  
Cultivar Identification ◽  
Barley Cultivar ◽  
Hordeum Vulgare L ◽  
Banding Patterns ◽  
Electrophoretic Patterns ◽  
Peroxidase Isozymes ◽  
Significant Relationships ◽  
Complete Identification

Electrophoresis was employed to study esterase, acid phosphatase and peroxidase isozymes in shoot extracts from four local and eight introduced barley (Hordeum vulgare L.) cultivars. The numbers of bands obtained for esterase, phosphatase, anodal peroxidase and cathodal peroxidase systems were 21, 11, 10 and 7, respectively. Results indicated that esterase alone could be used to differentiate between most of the cultivars and whenever it failed to show differences in banding patterns between two or more cultivars, either phosphatase or anodal peroxidase systems could be applied for complete identification. No significant relationships were found between the banding patterns and agronomic characteristics of the cultivars. The results are compared with other published reports.

Acid phosphatase isoforms in dry seeds and during seedling development in barley (Hordeum vulgare)

1996 ◽  
Vol 74 (5) ◽  
pp. 653-658
Author(s):  
S. Pasqualini ◽  
P. Batini ◽  
L. Ederli ◽  
F. Panara ◽  
M. Antonielli
Keyword(s):  
Cell Wall ◽  
Acid Phosphatase ◽  
Hordeum Vulgare ◽  
Developmental Stages ◽  
Soluble Fraction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seedling Development ◽  
Rapid Decrease ◽  
Hordeum Vulgare L ◽  
Electrophoretic Patterns

The acid phosphatase activity in the soluble, membrane, and cell wall fractions from Hordeum vulgare in dry seeds and during seedling development was investigated. The acid phosphatase activities were also assayed in barley roots and coleoptiles at different developmental stages. Electrophoretic patterns of multiple acid phosphatases in seeds, endosperms and embryos, and growing roots and coleoptiles are shown. The enzyme activity shows a rapid decrease in both roots and coleoptiles during growth. Using nondenaturing polyacrylamide gel electrophoresis, multiple acid phosphatase forms were found in all the organs examined. However, no qualitative differences in the location of bands were observed between root and coleoptile extract at various stages of development. The coleoptile cell wall fraction showed an acid phosphatase form characterized by a very low electrophoretic mobility that was not found in the soluble fraction. Keywords: barley, Hordeum vulgare L., acid phosphatase, isoforms, seedlings growth.

PEROXIDASE ISOZYMES IN MATURE BARLEY KERNELS

1973 ◽  
Vol 53 (4) ◽  
pp. 705-713 ◽  
Author(s):  
D. E. LaBERGE ◽  
J. E. KRUGER ◽  
W. O. S. MEREDITH
Keyword(s):  
Hordeum Vulgare ◽  
Genetic Markers ◽  
Disc Electrophoresis ◽  
Barley Cultivar ◽  
Actual Number ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Peroxidase Isozymes ◽  
Cereal Breeding ◽  
Mode Of Inheritance

Fourteen cationic peroxidase isozymes were detected in mature barley (Hordeum vulgare L.) kernels using polyacrylamide slabs for disc electrophoresis at pH 4.75. The actual number of isozymes detected on electrophoretograms of a particular barley cultivar was dependent on the hydrogen donor used for staining the peroxidase isozymes. In this respect, 3-amino-9-ethyl carbazole and o-dianisidine were superior to guaiacol, catechol, and benzidine as donors for differentiating the isozymes. Barley cultivars were classified into three categories based on varietal differences of peroxidase isozyme patterns. Evidence is presented to suggest that peroxidase isozymes may be useful genetic markers in cereal breeding work, but the results were not sufficiently detailed to present a hypothesis on the mode of inheritance of the different isozymes.

DIAMOND BARLEY

1985 ◽  
Vol 65 (1) ◽  
pp. 215-217 ◽  
Author(s):  
MARTIN L. KAUFMANN ◽  
SOLOMON KIBITE
Keyword(s):  
Hordeum Vulgare ◽  
Research Station ◽  
Barley Cultivar ◽  
Maturity Group ◽  
Hordeum Vulgare L ◽  
Lodging Resistance ◽  
Cultivar Description ◽  
The Cross

Diamond is a six-rowed feed barley (Hordeum vulgare L.) developed at Agriculture Canada Research Station, Lacombe, Alberta, from the cross Galt/Unitan. It has large kernels and yields more than other cultivars in its maturity group. Diamond has adequate disease and lodging resistance to be grown anywhere in the traditional six-rowed barley areas of Western Canada.Key words: Hordeum vulgare, barley, feed barley, cultivar description

OAC ACTON WINTER BARLEY

1985 ◽  
Vol 65 (2) ◽  
pp. 449-450 ◽  
Author(s):  
E. REINBERGS
Keyword(s):  
Hordeum Vulgare ◽  
Winter Barley ◽  
Science Department ◽  
Barley Cultivar ◽  
Hordeum Vulgare L ◽  
Lodging Resistance ◽  
Crop Science ◽  
Cultivar Description ◽  
Breeder Seed

OAC Acton is a new six-rowed winter barley (Hordeum vulgare L.) with good lodging resistance, higher yield, and better scald and BYD resistance than OAC Halton, the currently recommended winter barley cultivar for Ontario. It was licensed on 2 Aug. 1984. Breeder seed of OAC Acton is maintained by the Crop Science Department, University of Guelph, Guelph, Ontario.Key words: Hordeum vulgare L., barley (winter), BYD resistance, scald resistance, cultivar description

ANATOMICAL DISTRIBUTION OF PEROXIDASE ISOZYMES IN BARLEY KERNELS

1975 ◽  
Vol 55 (3) ◽  
pp. 661-666 ◽  
Author(s):  
D. E. LABERGE
Keyword(s):  
Anatomical Location ◽  
Barley Cultivar ◽  
Hordeum Vulgare L ◽  
Anatomical Distribution ◽  
Peroxidase Isozyme ◽  
Isozyme Patterns ◽  
Peroxidase Isozymes ◽  
Hydrogen Donors ◽  
Green Malt ◽  
Peroxidase Enzymes

Two barley (Hordeum vulgare L.) cultivars with different peroxidase isozyme patterns were studied using polyacrylamide-slab electrophoresis at pH 4.75 to separate the enzymes, and 3-amino-9-ethyl carbazole or o-dianisidine as hydrogen donors to detect peroxidase isozymes. Peroxidase isozyme patterns of extracts of very immature kernels up to 19 days post-anthesis were quite different from isozyme patterns from extracts of more mature kernels. During malting, the peroxidase isozymes of mature barely persisted in green malt, but an additional isozyme was detected in malt after 3 days of germination. Immature kernels with peroxidase isozyme patterns identical to those found in mature kernels for each barley cultivar were dissected into different tissue fractions including husks, pericarp, "green layer," aleurone, endosperm, embryo and scutellum. Electrophoresis of extracts of these tissues revealed the anatomical location of most of the peroxidase enzymes in the whole kernels of the two cultivars.

scholarly journals Studies on the genetic diversity of pointed gourd using biochemical methods (Isozyme analysis)

1970 ◽  
Vol 34 (1) ◽  
pp. 123-141
Author(s):  
ASMMR Khan ◽  
MG Rabbani ◽  
MA Siddique ◽  
MA Islam
Keyword(s):  
Genetic Diversity ◽  
Acid Phosphatase ◽  
Key Words ◽  
Glutamate Oxaloacetate Transaminase ◽  
Banding Patterns ◽  
Biochemical Methods ◽  
Electrophoretic Patterns ◽  
Wide Range ◽  
Rf Values ◽  
Pointed Gourd

Biochemical characterizations of 64 pointedgourds were done using three isozyme viz., acid phosphatase, peroxidase and glutamate oxaloacetate transaminase. A wide range of diversity among the gremplasm based on their acid phosphatase, peroxidase and glutamate oxaloacetate transaminase isoenzyme banding patterns were observed. In respect of isoenzyme activity; 8 acid phosphatase, 7 peroxidase and 9 glutamate oxaloacetate transminase electrophoretic zymotypes were formed by 19, 11, and 19 bands at different Rf values varying from 0.19 to 0.82, 0.38 to 0.69 and 0.15 to 0.95, respectively. The wide range of similarity co-efficient of 0.0-80.0, 0.0-66.0, and 0.0-80.0 as found among the electrophoretic patterns in acid phosphatase, peroxidase, and glutamate oxaloacetate transminase, respectively, indicating wide genetic diversity among the accessions. Based on the polymorphic activity of these three enzymes, 27 combinations of electrophoretic zymotypes were identified, each of which can he equated to genotypes. Each of the groups consisted of one to eight genotypes. Sixty four accessions of pointed gourd were grouped into 12 clusters. The genotypes collected from the same location were grouped into different clusters. Key Words: Genetic diversity; pointed gourd; biochemical methods. DOI: 10.3329/bjar.v34i1.5762Bangladesh J. Agril. Res. 34(1) : 123-141, March 2009

OAC HALTON WINTER BARLEY

1980 ◽  
Vol 60 (2) ◽  
pp. 741-742
Author(s):  
E. REINBERGS
Keyword(s):  
Disease Resistance ◽  
Hordeum Vulgare ◽  
Winter Barley ◽  
Science Department ◽  
Barley Cultivar ◽  
Hordeum Vulgare L ◽  
Lodging Resistance ◽  
Crop Science ◽  
Breeder Seed

OAC Halton is a new six-rowed winter barley (Hordeum vulgare L.) with good lodging resistance, higher yield, larger kernels and better disease resistance than Huron, the presently recommended winter barley cultivar for Ontario. It was licensed on 31 July 1979. Breeder seed of OAC Halton is maintained by the Crop Science Department, University of Guelph, Guelph, Ontario.

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