PEROXIDASE ISOZYMES IN MATURE BARLEY KERNELS

1973 ◽  
Vol 53 (4) ◽  
pp. 705-713 ◽  
Author(s):  
D. E. LaBERGE ◽  
J. E. KRUGER ◽  
W. O. S. MEREDITH
Keyword(s):  
Hordeum Vulgare ◽  
Genetic Markers ◽  
Disc Electrophoresis ◽  
Barley Cultivar ◽  
Actual Number ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Peroxidase Isozymes ◽  
Cereal Breeding ◽  
Mode Of Inheritance

Fourteen cationic peroxidase isozymes were detected in mature barley (Hordeum vulgare L.) kernels using polyacrylamide slabs for disc electrophoresis at pH 4.75. The actual number of isozymes detected on electrophoretograms of a particular barley cultivar was dependent on the hydrogen donor used for staining the peroxidase isozymes. In this respect, 3-amino-9-ethyl carbazole and o-dianisidine were superior to guaiacol, catechol, and benzidine as donors for differentiating the isozymes. Barley cultivars were classified into three categories based on varietal differences of peroxidase isozyme patterns. Evidence is presented to suggest that peroxidase isozymes may be useful genetic markers in cereal breeding work, but the results were not sufficiently detailed to present a hypothesis on the mode of inheritance of the different isozymes.

DIFFERENTIAL TOLERANCE OF TWO BARLEY CULTIVARS TO METRIBUZIN

1985 ◽  
Vol 65 (2) ◽  
pp. 415-421 ◽  
Author(s):  
C. D. CALDWELL ◽  
P. A. O’SULLIVAN
Keyword(s):  
Hordeum Vulgare ◽  
Relative Humidity ◽  
Opposite Effect ◽  
Dark Period ◽  
Light Period ◽  
Barley Cultivar ◽  
Herbicide Application ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Dark Regime

In greenhouse and growth cabinet studies, two cultivars of barley (Hordeum vulgare L. ’Klondike’ and ’Summit’) were found to differ in their tolerance to postemergence applications of metribuzin. Klondike was more susceptible than Summit, although both displayed varying amounts of injury depending on environmental conditions. Klondike was most susceptible following herbicide application at the three-leaf stage; when soil was moist at application; and when application was followed by a period of cold (0 °C). For both cultivars, high relative humidity and increasing duration of a dark period following spraying increased the injury from metribuzin. Increasing the duration of a light period following spraying had the opposite effect. The magnitude of the light/dark regime response was greater for Klondike than for Summit.Key words: Barley cultivar tolerance, metribuzin, environmental factors

BARLEY CULTIVAR IDENTIFICATION BY USE OF ISOZYME ELECTROPHORETIC PATTERNS

1976 ◽  
Vol 56 (1) ◽  
pp. 1-6 ◽  
Author(s):  
ABDOLLAH BASSIRI
Keyword(s):  
Acid Phosphatase ◽  
Hordeum Vulgare ◽  
Cultivar Identification ◽  
Barley Cultivar ◽  
Hordeum Vulgare L ◽  
Banding Patterns ◽  
Electrophoretic Patterns ◽  
Peroxidase Isozymes ◽  
Significant Relationships ◽  
Complete Identification

Electrophoresis was employed to study esterase, acid phosphatase and peroxidase isozymes in shoot extracts from four local and eight introduced barley (Hordeum vulgare L.) cultivars. The numbers of bands obtained for esterase, phosphatase, anodal peroxidase and cathodal peroxidase systems were 21, 11, 10 and 7, respectively. Results indicated that esterase alone could be used to differentiate between most of the cultivars and whenever it failed to show differences in banding patterns between two or more cultivars, either phosphatase or anodal peroxidase systems could be applied for complete identification. No significant relationships were found between the banding patterns and agronomic characteristics of the cultivars. The results are compared with other published reports.

Classification of cultivated barley (Hordeum vulgare). 1. Historical aspects, and phenetic character analysis of some characters by information theory and by spatial autocorrelation

1986 ◽  
Vol 64 (11) ◽  
pp. 2769-2773
Author(s):  
Bernard B. Baum
Keyword(s):  
Information Theory ◽  
Hordeum Vulgare ◽  
Spatial Autocorrelation ◽  
Theory Model ◽  
Kernel Weight ◽  
Hordeum Vulgare L ◽  
Department Of Agriculture ◽  
Character Analysis ◽  
Barley Cultivars

A brief historical sketch of the classification of barley (Hordeum vulgare L.) cultivars is presented along with reference to key reviews on this subject. Characters, utilized in the comprehensive study on the barley cultivars of North America by Aberg and Wiebe (U.S. Department of Agriculture Technical Bulletin 942), were subjected to a series of phenetic character analyses using an information theory model and a spatial autocorrelation model. The ranking of the 48 characters in order of their importance (for classification and identification purposes) from the character analysis by information theory was compared with the previous rating of characters made by Aberg and Wiebe and was found to differ significantly. Numerous trials of character analysis by spatial autocorrelation using various Minkowski distances, setting various values among three parameters, never yielded results comparable with those obtained by Aberg and Wiebe. Among those trials, a few combinations of values for the three parameters (X, Y, and Z) yielded results comparable with those obtained with character analysis by information theory. Those same combinations of values were found by Estabrook and Gates (Taxon, 33: 13–25) in their study of Banisteriopsis in 1984, where they also developed the method of character analysis by spatial autocorrelation. Kernel weight was found to be the most important character.

DIAMOND BARLEY

1985 ◽  
Vol 65 (1) ◽  
pp. 215-217 ◽  
Author(s):  
MARTIN L. KAUFMANN ◽  
SOLOMON KIBITE
Keyword(s):  
Hordeum Vulgare ◽  
Research Station ◽  
Barley Cultivar ◽  
Maturity Group ◽  
Hordeum Vulgare L ◽  
Lodging Resistance ◽  
Cultivar Description ◽  
The Cross

Diamond is a six-rowed feed barley (Hordeum vulgare L.) developed at Agriculture Canada Research Station, Lacombe, Alberta, from the cross Galt/Unitan. It has large kernels and yields more than other cultivars in its maturity group. Diamond has adequate disease and lodging resistance to be grown anywhere in the traditional six-rowed barley areas of Western Canada.Key words: Hordeum vulgare, barley, feed barley, cultivar description

OAC ACTON WINTER BARLEY

1985 ◽  
Vol 65 (2) ◽  
pp. 449-450 ◽  
Author(s):  
E. REINBERGS
Keyword(s):  
Hordeum Vulgare ◽  
Winter Barley ◽  
Science Department ◽  
Barley Cultivar ◽  
Hordeum Vulgare L ◽  
Lodging Resistance ◽  
Crop Science ◽  
Cultivar Description ◽  
Breeder Seed

OAC Acton is a new six-rowed winter barley (Hordeum vulgare L.) with good lodging resistance, higher yield, and better scald and BYD resistance than OAC Halton, the currently recommended winter barley cultivar for Ontario. It was licensed on 2 Aug. 1984. Breeder seed of OAC Acton is maintained by the Crop Science Department, University of Guelph, Guelph, Ontario.Key words: Hordeum vulgare L., barley (winter), BYD resistance, scald resistance, cultivar description

A comparative study of initiation and development of embryogenic callus from haploid embryos of several barley cultivars. II. Cytophotometry of embryos and callus

1986 ◽  
Vol 64 (9) ◽  
pp. 2107-2112 ◽  
Author(s):  
L. S. Kott ◽  
S. Flack ◽  
K. J. Kasha
Keyword(s):  
Hordeum Vulgare ◽  
Comparative Study ◽  
Ploidy Level ◽  
Embryogenic Callus ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Haploid Embryos ◽  
Diploid Level

Cells of haploid barley embryos (Hordeum vulgare L. 'Bruce', 'Perth', and 'Klages') and callus cells originating from embryos were cytophotometrically examined to determine the ploidy level. Specific embryo tissues regularly exhibited predictable ploidies and smaller embryos had a higher percentage of haploid cells than larger embryos of the same age. The predominantly haploid cells of the scutellar epidermis of the embryo initiated callus which generally, at least initially, was haploid. Monitoring of the ploidy evolution of these haploid calli showed that over a 6-month period each line exhibited its own unique rate of polyploidization, although lines of the same cultivar showed similar trends. Accumulation of cells at the diploid level was often a characteristic of these cultures.

ANATOMICAL DISTRIBUTION OF PEROXIDASE ISOZYMES IN BARLEY KERNELS

1975 ◽  
Vol 55 (3) ◽  
pp. 661-666 ◽  
Author(s):  
D. E. LABERGE
Keyword(s):  
Anatomical Location ◽  
Barley Cultivar ◽  
Hordeum Vulgare L ◽  
Anatomical Distribution ◽  
Peroxidase Isozyme ◽  
Isozyme Patterns ◽  
Peroxidase Isozymes ◽  
Hydrogen Donors ◽  
Green Malt ◽  
Peroxidase Enzymes

Two barley (Hordeum vulgare L.) cultivars with different peroxidase isozyme patterns were studied using polyacrylamide-slab electrophoresis at pH 4.75 to separate the enzymes, and 3-amino-9-ethyl carbazole or o-dianisidine as hydrogen donors to detect peroxidase isozymes. Peroxidase isozyme patterns of extracts of very immature kernels up to 19 days post-anthesis were quite different from isozyme patterns from extracts of more mature kernels. During malting, the peroxidase isozymes of mature barely persisted in green malt, but an additional isozyme was detected in malt after 3 days of germination. Immature kernels with peroxidase isozyme patterns identical to those found in mature kernels for each barley cultivar were dissected into different tissue fractions including husks, pericarp, "green layer," aleurone, endosperm, embryo and scutellum. Electrophoresis of extracts of these tissues revealed the anatomical location of most of the peroxidase enzymes in the whole kernels of the two cultivars.

Comparative proteomic analysis of hulless barley cultivars (Hordeum vulgare L.) differing distinctly in β-glucan content

LWT ◽  
2020 ◽  
Vol 133 ◽  
pp. 110085
Author(s):  
Guoqiang Zhang ◽  
Kun Yang ◽  
Wenhua Xue ◽  
Xingquan Zeng ◽  
Qijun Xu ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Proteomic Analysis ◽  
Hordeum Vulgare L ◽  
Hulless Barley ◽  
Barley Cultivars ◽  
Comparative Proteomic Analysis

RELATIONSHIP OF ADVENTITIOUS ROOTS AND AGRONOMIC CHARACTERISTICS IN BARLEY

1986 ◽  
Vol 66 (2) ◽  
pp. 257-280 ◽  
Author(s):  
E. A. HOCKETT
Keyword(s):  
Hordeum Vulgare ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Number ◽  
Seeding Rate ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Agronomic Characteristics ◽  
Relationship Of ◽  
Selection Of

The number of adventitious roots of two- and six-row barleys (Hordeum vulgare L.) grown under irrigation at Bozeman, Montana in 1970 and 1971, was related to yield and other agronomic characteristics. Adventitious roots were counted shortly before heading and at maturity and correlated with 15 agronomic characteristics. Adventitious root number was not correlated with yield of barley, except for two out of six cases in two-row barley. Roots per tiller, per plant and per square metre were all positively correlated with each other. The number of kernels per spike increased as roots per tiller increased but decreased with a high number of tillers per plant. Kernel plumpness and numbers of tillers were negatively correlated in two-row barley, but not in six-row barley. Cultivars differed significantly in yield and adventitious root number. Six-row barley had more roots per tiller but fewer tillers per square metre than two-row barley. Roots per tiller and roots per square metre measurements had the best repeatability between years for six-row barley, but no root measurements were consistent over years for the two-row type. Tillers per plant measurements for both two-row and six-row barleys and tillers per square metre for just two-row barley were consistent over years. Tiller and root number interact and are usually positively related. The genetic variation for root number shown here could be used in crosses for selection of superior genotypes.Key words: Barley, Hordeum vulgare, adventitious roots, subcrown internode, yield components, seeding rate

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