ANATOMICAL DISTRIBUTION OF PEROXIDASE ISOZYMES IN BARLEY KERNELS

1975 ◽  
Vol 55 (3) ◽  
pp. 661-666 ◽  
Author(s):  
D. E. LABERGE
Keyword(s):  
Anatomical Location ◽  
Barley Cultivar ◽  
Hordeum Vulgare L ◽  
Anatomical Distribution ◽  
Peroxidase Isozyme ◽  
Isozyme Patterns ◽  
Peroxidase Isozymes ◽  
Hydrogen Donors ◽  
Green Malt ◽  
Peroxidase Enzymes

Two barley (Hordeum vulgare L.) cultivars with different peroxidase isozyme patterns were studied using polyacrylamide-slab electrophoresis at pH 4.75 to separate the enzymes, and 3-amino-9-ethyl carbazole or o-dianisidine as hydrogen donors to detect peroxidase isozymes. Peroxidase isozyme patterns of extracts of very immature kernels up to 19 days post-anthesis were quite different from isozyme patterns from extracts of more mature kernels. During malting, the peroxidase isozymes of mature barely persisted in green malt, but an additional isozyme was detected in malt after 3 days of germination. Immature kernels with peroxidase isozyme patterns identical to those found in mature kernels for each barley cultivar were dissected into different tissue fractions including husks, pericarp, "green layer," aleurone, endosperm, embryo and scutellum. Electrophoresis of extracts of these tissues revealed the anatomical location of most of the peroxidase enzymes in the whole kernels of the two cultivars.

BARLEY CULTIVAR IDENTIFICATION BY USE OF ISOZYME ELECTROPHORETIC PATTERNS

1976 ◽  
Vol 56 (1) ◽  
pp. 1-6 ◽  
Author(s):  
ABDOLLAH BASSIRI
Keyword(s):  
Acid Phosphatase ◽  
Hordeum Vulgare ◽  
Cultivar Identification ◽  
Barley Cultivar ◽  
Hordeum Vulgare L ◽  
Banding Patterns ◽  
Electrophoretic Patterns ◽  
Peroxidase Isozymes ◽  
Significant Relationships ◽  
Complete Identification

Electrophoresis was employed to study esterase, acid phosphatase and peroxidase isozymes in shoot extracts from four local and eight introduced barley (Hordeum vulgare L.) cultivars. The numbers of bands obtained for esterase, phosphatase, anodal peroxidase and cathodal peroxidase systems were 21, 11, 10 and 7, respectively. Results indicated that esterase alone could be used to differentiate between most of the cultivars and whenever it failed to show differences in banding patterns between two or more cultivars, either phosphatase or anodal peroxidase systems could be applied for complete identification. No significant relationships were found between the banding patterns and agronomic characteristics of the cultivars. The results are compared with other published reports.

PEROXIDASE ISOZYMES IN MATURE BARLEY KERNELS

1973 ◽  
Vol 53 (4) ◽  
pp. 705-713 ◽  
Author(s):  
D. E. LaBERGE ◽  
J. E. KRUGER ◽  
W. O. S. MEREDITH
Keyword(s):  
Hordeum Vulgare ◽  
Genetic Markers ◽  
Disc Electrophoresis ◽  
Barley Cultivar ◽  
Actual Number ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Peroxidase Isozymes ◽  
Cereal Breeding ◽  
Mode Of Inheritance

Fourteen cationic peroxidase isozymes were detected in mature barley (Hordeum vulgare L.) kernels using polyacrylamide slabs for disc electrophoresis at pH 4.75. The actual number of isozymes detected on electrophoretograms of a particular barley cultivar was dependent on the hydrogen donor used for staining the peroxidase isozymes. In this respect, 3-amino-9-ethyl carbazole and o-dianisidine were superior to guaiacol, catechol, and benzidine as donors for differentiating the isozymes. Barley cultivars were classified into three categories based on varietal differences of peroxidase isozyme patterns. Evidence is presented to suggest that peroxidase isozymes may be useful genetic markers in cereal breeding work, but the results were not sufficiently detailed to present a hypothesis on the mode of inheritance of the different isozymes.

scholarly journals Genome Assembly of the Canadian Two-row Malting Barley Cultivar AAC Synergy

2021 ◽  
Author(s):  
Wayne Xu ◽  
James R Tucker ◽  
Wubishet A Bekele ◽  
Frank M You ◽  
Yong-Bi Fu ◽  
...  
Keyword(s):  
Reference Genome ◽  
Single Copy ◽  
Barley Cultivar ◽  
Malting Barley ◽  
Orthologous Genes ◽  
Hordeum Vulgare L ◽  
Chromosome Conformation ◽  
Mate Pair ◽  
Genome Assemblies ◽  
First Time

Abstract Barley (Hordeum vulgare L.) is one of the most important global crops. The six-row barley cultivar Morex reference genome has been used by the barley research community worldwide. However, this reference genome can have limitations when used for genomic and genetic diversity analysis studies, gene discovery, and marker development when working in two-row germplasm that is more common to Canadian barley. Here we assembled, for the first time, the genome sequence of a Canadian two-row malting barley, cultivar AAC Synergy. We applied deep Illumina paired-end reads, long mate-pair reads, PacBio sequences, 10X chromium linked read libraries, and chromosome conformation capture sequencing (Hi-C) to generate a contiguous assembly. The genome assembled from super-scaffolds had a size of 4.85 Gb, N50 of 2.32 Mb and an estimated 93.9% of complete genes from a plant database (BUSCO, benchmarking universal single-copy orthologous genes). After removal of small scaffolds (< 300 Kb), the assembly was arranged into pseudomolecules of 4.14 Gb in size with seven chromosomes plus unanchored scaffolds. The completeness and annotation of the assembly were assessed by comparing it with the updated version of six-row Morex and recently released two-row Golden Promise genome assemblies.

DIAMOND BARLEY

1985 ◽  
Vol 65 (1) ◽  
pp. 215-217 ◽  
Author(s):  
MARTIN L. KAUFMANN ◽  
SOLOMON KIBITE
Keyword(s):  
Hordeum Vulgare ◽  
Research Station ◽  
Barley Cultivar ◽  
Maturity Group ◽  
Hordeum Vulgare L ◽  
Lodging Resistance ◽  
Cultivar Description ◽  
The Cross

Diamond is a six-rowed feed barley (Hordeum vulgare L.) developed at Agriculture Canada Research Station, Lacombe, Alberta, from the cross Galt/Unitan. It has large kernels and yields more than other cultivars in its maturity group. Diamond has adequate disease and lodging resistance to be grown anywhere in the traditional six-rowed barley areas of Western Canada.Key words: Hordeum vulgare, barley, feed barley, cultivar description

OAC ACTON WINTER BARLEY

1985 ◽  
Vol 65 (2) ◽  
pp. 449-450 ◽  
Author(s):  
E. REINBERGS
Keyword(s):  
Hordeum Vulgare ◽  
Winter Barley ◽  
Science Department ◽  
Barley Cultivar ◽  
Hordeum Vulgare L ◽  
Lodging Resistance ◽  
Crop Science ◽  
Cultivar Description ◽  
Breeder Seed

OAC Acton is a new six-rowed winter barley (Hordeum vulgare L.) with good lodging resistance, higher yield, and better scald and BYD resistance than OAC Halton, the currently recommended winter barley cultivar for Ontario. It was licensed on 2 Aug. 1984. Breeder seed of OAC Acton is maintained by the Crop Science Department, University of Guelph, Guelph, Ontario.Key words: Hordeum vulgare L., barley (winter), BYD resistance, scald resistance, cultivar description

Peroxidase isozyme patterns in primary trisomics of pearl millet

1984 ◽  
Vol 68-68 (1-2) ◽  
pp. 179-182 ◽  
Author(s):  
J. S. Sidhu ◽  
J. S. Ravi ◽  
J. L. Minocha
Keyword(s):  
Pearl Millet ◽  
Peroxidase Isozyme ◽  
Primary Trisomics ◽  
Isozyme Patterns

Involvement of a novel peroxidase isozyme and lignification in hydathodes in resistance to black rot disease in cabbage

2000 ◽  
Vol 78 (9) ◽  
pp. 1144-1149 ◽  
Author(s):  
P A Gay ◽  
S Tuzun
Keyword(s):  
Xanthomonas Campestris ◽  
Virulent Strain ◽  
Black Rot ◽  
Peroxidase Isozyme ◽  
Anionic Peroxidase ◽  
Peroxidase Isozymes ◽  
Resistant Varieties ◽  
Rot Disease ◽  
Xanthomonas Campestris Pv Campestris ◽  
Lignin Deposition

The physiological mechanisms associated with resistance of cabbage to black rot disease seem to be associated with the hydathodes. To investigate the role of hydathodes in disease resistance, total peroxidase activities, anionic peroxidase isozyme expression, and lignin deposition were determined in hydathodes of resistant (Hancock and Green Cup), partially resistant (Cheers), and susceptible (Strukton and Perfect Ball) cabbage varieties (Brassica oleracea L.) during pathogenesis with Xanthomonas campestris pv. campestris. Four-week-old plants were petiole-inoculated with a highly virulent strain of X. campestris pv. campestris (FD91L). Hydathodal fluids were collected daily over a 14-day period from infected plants as well as noninfected, mock-inoculated control plants. Hydathodal fluids of resistant varieties had greater peroxidase activity when compared to susceptible ones, with infected plants having higher peroxidase levels than noninfected plants. Isoelectric focusing revealed the presence of four anionic peroxidase isozymes in hydathodal fluids, with the most anionic one (pI of 3.6) accumulating only upon infection. Lignin deposition in and around the hydathodes was associated with the accumulation of this particular isozyme in hydathodal fluids. The evidence suggests that a rapid, systemic response is associated with resistance to the bacterial pathogen.Key words: peroxidases, hydathodes, isozymes, black rot disease, cabbage, Xanthomonas campestris pv. campestris.

scholarly journals Peroxidase Isozyme Profiles in Compatible and Incompatible Pear-Quince Graft Combinations

2002 ◽  
Vol 127 (2) ◽  
pp. 152-157 ◽  
Author(s):  
Hatice Gulen ◽  
Rajeev Arora ◽  
Ali Kuden ◽  
Stephen L. Krebs ◽  
Joseph Postman
Keyword(s):  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Field Observations ◽  
Cydonia Oblonga ◽  
Graft Union ◽  
Peroxidase Isozyme ◽  
Peroxidase Isozymes ◽  
Starch Gel ◽  
Graft Compatibility ◽  
Growth Vigor

The similarity or differences of peroxidase isozymes in rootstocks and scions may influence their graft compatibility. This study was conducted to identify peroxidase isozymes that may be used as markers to predict compatibility between pear (Pyrus communis L.) and various quince (Cydonia oblonga Mill.) clones. `Bartlett' (BT) and `Beurre Hardy' (BH) pear cultivars are known to form incompatible and compatible grafts, respectively, with quince rootstocks. The two pear scion cultivars were budded on `quince A' (QA), `quince BA-29', and 15 selected quince clones from Turkey. Bark and cambial tissues were taken from nonbudded rootstocks and scions, and 4 cm above and below the graft union for peroxidase isozyme analysis performed by starch gel electrophoresis. Isoperoxidase analyses were also performed on samples from the graft unions collected 12 months after grafting. Many isozyme bands were observed commonly in the two scions; however, one anodal peroxidase A was detected in BH (compatible scion) but not in BT (incompatible scion) samples. This isoperoxidase was also detected in QA, Quince BA-29, and nine of the Turkish quince clones. Another isoperoxidase, band B, was detected in BH but not in BT or any of the rootstocks. However, the compatible (BH/QA) and moderately compatible (BT/BA-29) graft union tissues contained bands A and B whereas incompatible graft union tissues (BT/QA) lacked both. Graft union samples involving BT and five Turkish quince clones (705, 609-2, 702, 804, and 806) had both `A' and `B' isoperoxidases while one or both of these bands were absent in nonbudded graft partners. Field observations of 3.5 year-old grafts of BT and Turkish quince clones revealed that the vegetative growth (vigor) of BT scion was significantly greater, when grafted on these five clones, than that in graft combinations with other clones. We suggest that matching of isoperoxidase `A' in quince rootstocks and BH pear scion may be associated with a compatible graft combination. Additionally, presence of isoperoxidases `A' and `B' in the graft union tissues may be used as an indicator to predict a compatible graft between BT and quince rootstocks.

Epidemiology of Hand Fractures and Dislocations in England and Wales Professional Cricketers

2021 ◽  
Author(s):  
Sandeep Rajiv Deshmukh ◽  
Ella Donnison ◽  
Alexia Karantana ◽  
David Newman ◽  
Nicholas Peirce
Keyword(s):  
Index Finger ◽  
Anatomical Location ◽  
Injury Incidence ◽  
Anatomical Distribution ◽  
England And Wales ◽  
Hand Fractures ◽  
Hand Fracture ◽  
Playing Time ◽  
Injury Burden ◽  
And Training

AbstractThis study describes hand fracture and dislocation injuries in terms of anatomical distribution, incidence and impact on playing time in registered professional adult male players of all 18 First Class England and Wales County Cricket clubs over a five-year period from 2010–2014. Prospectively collected injury surveillance data for 1st and 2nd Team matches (Twenty20, One day and four-day) and training were analysed. There were 109 hand fractures and 53 dislocations. Hand injury was commonest during fielding (60%, 98/162) compared to batting, bowling or wicket-keeping. Exposed parts of the hand including tips of all digits, the index finger, thumb ray and little finger ray were most frequently injured with 78% (125/160) of all injuries where anatomical location was recorded. Match injury incidence for batsmen was highest in four-day matches (0.071 injuries per 1000 overs batted) but for other player roles it was highest in Twenty20 matches (0.587 per 1000 overs bowled). Player unavailability for selection to play was incurred in 82% (89/109) of hand fractures but only 47% (25/53) of dislocations. This study clarifies the hand fracture and dislocation injury burden for this population.

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