Peroxidase isozyme patterns in primary trisomics of pearl millet

1984 ◽  
Vol 68-68 (1-2) ◽  
pp. 179-182 ◽  
Author(s):  
J. S. Sidhu ◽  
J. S. Ravi ◽  
J. L. Minocha
1975 ◽  
Vol 55 (3) ◽  
pp. 661-666 ◽  
Author(s):  
D. E. LABERGE

Two barley (Hordeum vulgare L.) cultivars with different peroxidase isozyme patterns were studied using polyacrylamide-slab electrophoresis at pH 4.75 to separate the enzymes, and 3-amino-9-ethyl carbazole or o-dianisidine as hydrogen donors to detect peroxidase isozymes. Peroxidase isozyme patterns of extracts of very immature kernels up to 19 days post-anthesis were quite different from isozyme patterns from extracts of more mature kernels. During malting, the peroxidase isozymes of mature barely persisted in green malt, but an additional isozyme was detected in malt after 3 days of germination. Immature kernels with peroxidase isozyme patterns identical to those found in mature kernels for each barley cultivar were dissected into different tissue fractions including husks, pericarp, "green layer," aleurone, endosperm, embryo and scutellum. Electrophoresis of extracts of these tissues revealed the anatomical location of most of the peroxidase enzymes in the whole kernels of the two cultivars.


2000 ◽  
Vol 23 (4) ◽  
pp. 415-422 ◽  
Author(s):  
J. Andrews ◽  
M. Malone ◽  
D. S. Thompson ◽  
L. C. Ho ◽  
K. S. Burton

1988 ◽  
Vol 101 (3) ◽  
pp. 256-259 ◽  
Author(s):  
S. S. Chahal ◽  
R. Kumar ◽  
J. S. Sidhu ◽  
J. L. Minocha

1976 ◽  
Vol 18 (1) ◽  
pp. 11-15 ◽  
Author(s):  
V. Manga

Seven primary trisomics were obtained in the progeny of an autotriploid Pennisetum typhoides (Burm.) S. &H. The trisomics could be distinguished from the diploids and from one another morphologically and were designated Dwarf, Bush, Slender, Semidwarf, Purple, Robust and Pseudonormal. In Purple and Pseudonormal the mean chiasma frequencies were significantly higher than in the diploid sibs but in Dwarf the mean chiasma frequency was significantly lower. In the other trisomics the chiasma frequencies did not differ from the diploid sibs. The trivalent frequencies and configurations varied in the different trisomics and no correlation was noticed between the length of the chromosome and trivalent frequency but one was observed between trivalent frequency and chiasma frequency.


Author(s):  
É. Stefanovits-Bányai ◽  
S. Lakatos ◽  
M. Hajós-Novák ◽  
E. Hajdu ◽  
I. Balogh

Isoelectric focusing is an effective and well reproducible method to provide information for identification of various plant species and clones if breeding or other genetic modification(s) for a given species are reflected in changes of an isozyme pattern. The method has been used for characterization of plant proteins and enzymes and for identification of various species and varieties. Our aim was to continue our several-year-work carried out on a wide variety of grapevine varieties and to reveal whether analyses of esterase and peroxidase isozyme patterns are suitable to distinguish various grapevine varieties. Therefore we compared esterase and peroxidase isozyme patterns of various species. Plant samples were obtained from Szigetcsep, Kecskemet, Tokaj and Eger. The following samples were analyzed: Pinot gris, noir, blanc, Chardonnay, Riesling Theses, Chasselas from Szigetcs6p, Bianca and his parents Eger2 and Bouvier from Kecskemet, Furmint and Hárslevelű from Tokaj, Kékfrankos and Zweigelt from Eger. To identify various species according to their esterase isozyme patterns the after blooming phenological phase while according to their peroxidase isozyme pattern the dormant phenological phase was found as optimal sampling time.


1986 ◽  
Vol 4 (3) ◽  
pp. 101-107
Author(s):  
R.A. Menendez ◽  
F.E. Larsen ◽  
R. Fritts

Anionic electrophoretic isozyme patterns of peroxidase, esterase and acid phosphatase and cationic peroxidase isozyme patterns from shoot bark protein extracts were used to identify clonal apple scion cultivars. Each of the 21 cultivars included in this study developed a unique combination of isozyme patterns which allowed it to be distinguished from the others. Sports within cultivars exhibited identical patterns of enzymes, with the exception of ‘Wijcik’, a natural compact mutant of ‘McIntosh’ which could be distinguished from the latter, although it was indistinguishable from the cultivar ‘Spartan’. Isozyme patterns remained constant when samples were taken from wood of different ages, at several times of the year and with trees growing in different locations and on different rootstocks.


CYTOLOGIA ◽  
1982 ◽  
Vol 47 (3/4) ◽  
pp. 503-510 ◽  
Author(s):  
R. Sai Kumar ◽  
U. P. Singh ◽  
R. B. Singh ◽  
R. M. Singh

1984 ◽  
Vol 67 (2-3) ◽  
pp. 135-142 ◽  
Author(s):  
U. P. Singh ◽  
R. Sai Kumar ◽  
R. M. Singh ◽  
R. B. Singh

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