Post-weaning excretion of purine derivatives by young calves

1995 ◽  
Vol 75 (1) ◽  
pp. 153-156 ◽  
Author(s):  
M. Funaba ◽  
K. Kagiyama ◽  
T. Iriki ◽  
M. Abe
Keyword(s):  
Organic Matter ◽  
Key Words ◽  
Nitrogen Supply ◽  
Purine Derivatives ◽  
Purine Derivative ◽  
Microbial Nitrogen ◽  
Digestible Organic Matter

Intestinal flow of MN was estimated from urinary purine derivative output in calves. Although estimated MN output (g d−1) increased with age, it was restricted to the period between weeks 6 and 11 after weaning when expressed as g d−1 kg−0.75. The estimated MN output to digestible organic matter ratio decreased with time after weaning, although the cubic contrast indicated to be highest on wks 1 and 11. Key words: Microbial nitrogen supply, purine derivatives, growing calves

Effect of leucaena supplementation level, and provision of urea, on utilisation by sheep of Rhodes grass hay

2001 ◽  
Vol 41 (2) ◽  
pp. 155 ◽  
Author(s):  
I. W. Karda ◽  
G. McL. Dryden
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Latin Square ◽  
Rhodes Grass ◽  
Microbial Nitrogen ◽  
Grass Hay ◽  
Digestible Organic Matter ◽  
Ruminal Degradation ◽  
Chloris Gayana ◽  
Dietary Treatments

Tarramba leucaena (Leucaena leucocephala cv. Tarramba) foliage had per kilogram dry matter, 169 g protein and 29.8 g condensed tannins. Its value as a supplement, given either with or without urea, to sheep given a low-quality Callide Rhodes grass (Chloris gayana cv. Callide) hay was studied. Six rumen fistulated sheep (mean s.d. liveweight, 34 1.4 kg) were used to compare 6 dietary treatments in an incomplete latin square design. Rhodes grass hay was given ad libitum either alone, or with urea 7 g/day (U), or with leucaena 150 g/day (L150), or leucaena with urea (L150U), or leucaena 300 g/day (L300), or leucaena with urea (L300U). Digestible organic matter intake was increased significantly by leucaena supplementation although digestibility of the whole diet did not alter. Rumen fluid ammonia-N was not altered by leucaena supplementation, but was increased by urea. This suggests that Tarramba foliage protein has some resistance to ruminal degradation. Liquid and solids passage rates were not affected by the treatments. Microbial nitrogen supply to the intestine (g/day), and the efficiency of microbial nitrogen synthesis (g/kg organic matter apparently digested in the rumen), were increased by leucaena supplementation (P<0.01). Microbial protein synthesis was the only response in which 300 g/day air-dry Tarramba foliage gave improved results over 150 g/day.

Effect of amount of rumen degradable protein on the utilization of wheat straw by Dohne Merino wethers

2003 ◽  
Vol 76 (2) ◽  
pp. 319-326
Author(s):  
J. van E. Nolte ◽  
A.V. Ferreirat ◽  
H.H. Köster
Keyword(s):  
Organic Matter ◽  
Wheat Straw ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Microbial Nitrogen ◽  
Digestible Organic Matter ◽  
Microbial Efficiency ◽  
Forage Intake ◽  
Degradable Protein

AbstractA 5 ✕ 5 Latin square was conducted with ruminally and duodenally cannulated Dohne Merino wethers consuming wheat straw to determine the effect of different rumen degradable protein (RDP) levels on forage intake, fermentation characteristics, nutrient flow and digestion. The wethers had ad libitum access to water and wheat straw (32 g crude protein (CP) per kg dry matter (DM); 742 g neutral-detergent fibre (NDF) per kg DM) that was offered twice daily, immediately after intraruminal infusion of the supplements at 07:00 and 19:00 h. The supplemental RDP (calcium caseinate; 900 g CP per kg DM) levels were: 0, 40, 80, 120 and 160 g/day. Each period consisted of 14 days of adaptation and 6 days of sampling. Forage and total organic matter (OM) intakes increased in a linear manner (P < 0•01) with increasing supplemental RDP levels. Digestible organic matter intake (DOMI) displayed a quadratic increase with elevated amounts of RDP (P < 0•01). The effects of treatments on rumen and total tract digestion, as well as fluid dilution rate were minimal. Microbial nitrogen (MN) flow to the duodenum and microbial efficiency increased quadratically (P < 0•04) with increased RDP supplementation. Rumen ammonia nitrogen (NH3-N) concentrations increased linearly (P < 0•01) and total volatile fatty acids (VFA) tended to increase linearly (P = 0•07). In conclusion, RDP supplementation to Dohne Merino wethers consuming wheat straw generally enhanced rumen fermentation and forage intake. A total RDP intake (sources: calcium caseinate and wheat straw) of 3•30 g/kg M0•75 or 0•12 of DOM maximized DOMI.

Rumen microbial production estimated either from urinary purine derivative excretion or from direct measurements of 15N and purine bases as microbial markers: effect of protein source and rumen bacteria isolates

1997 ◽  
Vol 65 (2) ◽  
pp. 225-236 ◽  
Author(s):  
J. F. Pérez ◽  
J. Balcells ◽  
J. A. Guada ◽  
C. Castrillo
Keyword(s):  
Organic Matter ◽  
Urinary Excretion ◽  
Fish Meal ◽  
Soya Bean ◽  
Purine Derivatives ◽  
Sunflower Meal ◽  
Purine Bases ◽  
Direct Measurements ◽  
Digestible Organic Matter ◽  
Microbial N

AbstractFour ewes fitted with ruminal and duodenal T-piece cannulae were each given six diets in a 6 × 4 factorial design. Diets or experimental treatments consisted of two ratios of forage: concentrate (700:150 (LC) and 400: 600 (HO). Forage was ammonia-treated straw and the concentrate was formulated with barley supplemented with one of three protein sources: sunflower meal, soya-bean meal or fish meal. Duodenal flows ofdigesta were estimated by the dual-phase technique using Co-EDTA and Yb acetate as liquid and solid markers. Microbial nitrogen (N) was estimated from the digesta flow of purine bases and 15N enrichment using as reference samples, bacterial isolates from the liquid (LAB) or solid (SAB) phase of rumen digesta.Duodenal flow of purine bases (mmol/day) was lower on LC (12·9) than HC (17·7) diets but in both treatments it was depressed by fish meal (12·3) compared with either soya-bean (17·3) or sunflower meal (16·3) as supplements (s.e. 1·13). Urinary excretion of purine derivatives showed a similar trend, 8·6 v. III mmol/day in LC and HC respectively and 8·8 v. 10·4 and 10·5 mmol/day in fish meal, soya-bean and sunflower meal diets (s.e. 0·56), respectively. Variation in excretion of urinary purine derivatives was mainly associated with digestible organic matter intake with an average ratio of 1·7 (s.e. 0·11) mmol per 100 g digestible organic matter intake. Irrespective of the microbial marker used, microbial yield was higher in animals offered HC than in those offered LC and with soya-bean or sunflower meal compared with fish meal supplemented diets. The microbial purine bases/N (mmol/g) ratio varied between LAB (1·99, s.e. 0·092) and SAB (1·69, s.e. 0·071) isolates leading to different estimates of microbial-N yield (g) from duodenal purine bases (7·76 (s.e. 2·84) v. 9·13 (s.e. 3·24)), urinary excretion of allantoin (5·57 (s.e. 2·0) v. 6·57 (s.e. 2·03)) or total purine derivatives (6·43 (s.e. 2·39) v. 7·56 (s.e. 2·77)). Urinary excretion of allantoin or total purine derivatives provided consistently lower estimates of duodenal microbial-N than duodenal purine bases or 15N, although it closely reflected the pattern observed in direct measurements.

EFFECT OF DIGESTIBLE CARBOHYDRATES ON THE APPARENT ABSORPTION OF MAGNESIUM BY WETHER LAMBS

1984 ◽  
Vol 64 (5) ◽  
pp. 219-220
Author(s):  
J. K. THOMPSON ◽  
A. L. GELMAN ◽  
C. S. JESSIMAN
Keyword(s):  
Organic Matter ◽  
Key Words ◽  
Corn Starch ◽  
Apparent Absorption ◽  
Grass Silage ◽  
Digestible Organic Matter ◽  
Consistent Increase ◽  
Forage Diets

The apparent absorption of magnesium was found to increase with increasing intakes of digestible organic matter in young wether lambs fed on a variety of forage diets. The addition of corn starch to either grass or grass silage diets did not, however, give a consistent increase in magnesium absorption. Key words: Sheep, Magnesium, energy

scholarly journals The effect of ammoniation on the nutritive value of tall wheatgrass

1991 ◽  
Vol 71 (3) ◽  
pp. 867-870
Author(s):  
R. D. H. Cohen ◽  
K. A. Cruise ◽  
J. A. Kernan ◽  
E. C. Coxworth ◽  
J. E. Knipfel
Keyword(s):  
Organic Matter ◽  
Key Words ◽  
Protein Intake ◽  
Crude Protein ◽  
Nutritive Value ◽  
Agropyron Elongatum ◽  
Tall Wheatgrass ◽  
Digestible Organic Matter ◽  
Acid Detergent Fibre ◽  
Digestible Crude Protein

Tall wheatgrass (TWG, Agropyron elongatum (Host) Beauvois) was harvested at anthesis, ammoniated, fed to sheep and its nutritive value was compared with that of nonammoniated TWG. Ammoniation increased organic matter digestibility (OMD) from 59.2 to 65.9% (SEM = 1.45; P < 0.001), acid detergent fibre digestibility (ADFD) from 53.2 to 63.4% (SEM = 2.14; P < 0.001), neutral detergent fibre digestibility (NDFD) from 55.2 to 69.3% (SEM = 2.90; P < 0.001), digestible organic matter (DOMI) from 25.8 to 44.3 g kg−0.75 d−1 (SEM = 3.90; P < 0.001) and digestible crude protein intake (DCPI) from 1.33 to 5.97 g kg−0.75 d−1 (SEM = 0.97; P < 0.001). Key words: Tall wheatgrass, ammoniation, intake, digestibility, fibre, sheep

scholarly journals Excretion of endogenous and exogenous purine derivatives in sheep: effect of increased concentrate intake

1998 ◽  
Vol 79 (3) ◽  
pp. 237-240 ◽  
Author(s):  
J. F. Pérez ◽  
J. Balcells ◽  
J. A. Cebrián ◽  
S. M. Martín-Orúe
Keyword(s):  
Organic Matter ◽  
Uric Acid ◽  
Urinary Excretion ◽  
Isotope Dilution ◽  
Basal Diet ◽  
Barley Grain ◽  
Dual Phase ◽  
Purine Derivatives ◽  
Marker System ◽  
Digestible Organic Matter

The present study examined the endogenous urinary excretion of purine derivatives (PD; allantoin, uric acid and xanthine plus hypoxanthine) in fed animals. Four Rasa Aragonesa ewes fitted with simple cannulas in the rumen and proximal duodenum were used. Animals were given a lucerne (Medicago sativa) hay diet, as sole feed (A) or supplemented, respectively, with 220 (B), 400 (C), and 550 (D) g rolled barley grain/d following a 4 × 4 random factorial design. Duodenal flow of purine bases (PB) was determined by the dual-phase marker system. 15N was infused continuously into the rumen to label exogenous or microbial PB. Duodenal PB flow and urinary excretion of PD increased with digestible organic matter intake showing a constant recovery of duodenal PB. The isotope dilution of PD in urine samples confirmed the presence of an endogenous fraction, originating from tissues, that increased from 115.2 (SE 5.84) μmol/kg W0.75 for the basal diet to 304.2 (SE 7.6) μmol/kg W0.75 at the highest level of duodenal PB.

scholarly journals Soil nitrogen supply of peat grasslands estimated by degree days and soil organic matter content

2020 ◽  
Vol 117 (3) ◽  
pp. 351-365
Author(s):  
J. Pijlman ◽  
G. Holshof ◽  
W. van den Berg ◽  
G. H. Ros ◽  
J. W. Erisman ◽  
...  
Keyword(s):  
Organic Matter ◽  
Soil Organic Matter ◽  
Soil Nitrogen ◽  
Organic Matter Content ◽  
Matter Content ◽  
Nitrogen Supply ◽  
Degree Days ◽  
Soil Organic Matter Content ◽  
Soil Nitrogen Supply
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