scholarly journals Effect of rumen degradable protein (RDP) in straw based ration on purine derivatives excretion and microbial nitrogen supply in cattle

1997 ◽  
Vol 10 (4) ◽  
pp. 364-370 ◽  
Author(s):  
Z. H. Khandaker ◽  
A. M. M. Tareque
Keyword(s):  
Nitrogen Supply ◽  
Purine Derivatives ◽  
Microbial Nitrogen ◽  
Degradable Protein

Post-weaning excretion of purine derivatives by young calves

1995 ◽  
Vol 75 (1) ◽  
pp. 153-156 ◽  
Author(s):  
M. Funaba ◽  
K. Kagiyama ◽  
T. Iriki ◽  
M. Abe
Keyword(s):  
Organic Matter ◽  
Key Words ◽  
Nitrogen Supply ◽  
Purine Derivatives ◽  
Purine Derivative ◽  
Microbial Nitrogen ◽  
Digestible Organic Matter

Intestinal flow of MN was estimated from urinary purine derivative output in calves. Although estimated MN output (g d−1) increased with age, it was restricted to the period between weeks 6 and 11 after weaning when expressed as g d−1 kg−0.75. The estimated MN output to digestible organic matter ratio decreased with time after weaning, although the cubic contrast indicated to be highest on wks 1 and 11. Key words: Microbial nitrogen supply, purine derivatives, growing calves

Effects of yeast culture on rumen fermentation and liveweight gain in bulls fed isonitrogenous diets of barley/urea or barley/soya

1992 ◽  
Vol 1992 ◽  
pp. 211-211
Author(s):  
S.M. Elhassan ◽  
R.J. Wallace ◽  
C.J. Newbold ◽  
X.B. Chen ◽  
I.E. Edwards ◽  
...  
Keyword(s):  
Microbial Growth ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Feed Additive ◽  
Yeast Culture ◽  
Purine Derivatives ◽  
Stomach Tube ◽  
Cane Molasses ◽  
Microbial Nitrogen ◽  
Soyabean Meal

Yeast culture (YC) based on Saccharomyces cerevisiae is gaining increasing acceptance as a feed additive for ruminants. Production responses to YC have been reported in growing and lactating animals. It has been suggested that these responses are at least partly due to enhanced microbial growth in and microbial nitrogen flow from the rumen (Williams and Newbold, 1990). The aim of the present study was to investigate the effect of YC on rumen fermentation, microbial growth and liveweight gain in bulls fed isonitrogenous diets containing either urea or soya.Thirty two Limousin cross Friesian bulls (liveweight 344 kg) were allocated to treatments on the basis of previous growth rate and initial liveweight. Animals received mixed diets of barley/urea (92% rolled barley, 5% cane molasses, 1.5% urea and 1.5% minerals/vitamin mix) or barley/soya (86.75% rolled barley, 5% cane molasses, 0.75% urea, 6% soyabean meal and 1.5% minerals/vitamin mix) plus or minus 1.5 kg/ tonne YC (Yea-sacc1026 , Alltech, UK) (Table 1). YC was added to the diet prior to mixing. All rations were offered ad libitum twice daily until slaughter (460 kg). Rumen fluid samples were withdrawn by stomach tube 5 and 10 weeks after the beginning of the trial. The outflow of microbial nitrogen from the rumen was estimated from the appearance of purine derivatives in the urine as described previously (Chen et al., 1990).

scholarly journals PSI-27 Excretion of purine derivatives and rumen microbial protein synthesis in Nelore steers supplemented with low-moisture, sugarcane molasses-based block and fed low-quality forage

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 249-250
Author(s):  
Andresa L Feliciano ◽  
Sérgio A G Pereira-Junior ◽  
Yury Granja-Salcedo ◽  
Livia Maria Sampaio Ferraz Sepini de Souza Grilo ◽  
Luís Felipe Arelaro Artioli ◽  
...  
Keyword(s):  
Random Effect ◽  
Latin Square ◽  
Experimental Period ◽  
Sugarcane Molasses ◽  
Microbial Protein ◽  
Purine Derivatives ◽  
Microbial Nitrogen ◽  
Protein Supply ◽  
Protein Block ◽  
Corn Grain

Abstract The objective of this study was to determine the effects of supplementation of low-moisture, sugarcane molasses-based block (LMB) on steers fed low quality forage in the excretion of purine derivatives and in the synthesis of ruminal microbial protein. Six rumen cannulated Nellore steers steers (23 months, 350 ± 10 kg) were distributed in a 3 × 3 double Latin square design. The treatments were composed of Brachiaria brizantha ‘Marandu’ hay ad libtum as an exclusive source of bulky (93.65% DM, 3.97% CP and 81.76% NDF) and supplements: complete mineral blend with urea [UR, (urea, salt, mineral-vitamin premix)], a commercial protein supplement [PS, (corn grain, soybean meal, urea, salt and mineral-vitamin premix)] or low-moisture, cooked sugarcane molasses-based protein block [LMB, (cane molasses, cottonseed meal, soybean oil, urea, salt and mineral-vitamin premix)]. Each experimental period lasted 21 days (14 days of adaptation and 7 days of data collection). The total urinary volume was measured for five days in each experimental period. The urine was collected in rubber funnels fixed by elastic loops on the backs of the animals. The urine was conduct through hoses connected to a 20 L polyethylene bucket containing 250 mL of 20% H2SO4 solution. Every 24 hours, the collected urine was homogenized and the total excreted volume was measured. The data were analyzed using Software R, having as fixed effect the treatments and as animal random effect, period, Latin square and error. Supplementation with LMB lead to greater excretion of allantoin (P = 0.046), microbial nitrogen flow (P = 0.023) and higher microbial crude protein (P = 0.023) into the intestine compared to UR and PS. While no effect was observed on total purines and purines absorbed (P > 0.05). Thus, LMB supplementation for rumen cannulated Nellore steers fed low quality forage was effective to improve metabolizable protein supply.

Effect of amount of rumen degradable protein on the utilization of wheat straw by Dohne Merino wethers

2003 ◽  
Vol 76 (2) ◽  
pp. 319-326
Author(s):  
J. van E. Nolte ◽  
A.V. Ferreirat ◽  
H.H. Köster
Keyword(s):  
Organic Matter ◽  
Wheat Straw ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Microbial Nitrogen ◽  
Digestible Organic Matter ◽  
Microbial Efficiency ◽  
Forage Intake ◽  
Degradable Protein

AbstractA 5 ✕ 5 Latin square was conducted with ruminally and duodenally cannulated Dohne Merino wethers consuming wheat straw to determine the effect of different rumen degradable protein (RDP) levels on forage intake, fermentation characteristics, nutrient flow and digestion. The wethers had ad libitum access to water and wheat straw (32 g crude protein (CP) per kg dry matter (DM); 742 g neutral-detergent fibre (NDF) per kg DM) that was offered twice daily, immediately after intraruminal infusion of the supplements at 07:00 and 19:00 h. The supplemental RDP (calcium caseinate; 900 g CP per kg DM) levels were: 0, 40, 80, 120 and 160 g/day. Each period consisted of 14 days of adaptation and 6 days of sampling. Forage and total organic matter (OM) intakes increased in a linear manner (P < 0•01) with increasing supplemental RDP levels. Digestible organic matter intake (DOMI) displayed a quadratic increase with elevated amounts of RDP (P < 0•01). The effects of treatments on rumen and total tract digestion, as well as fluid dilution rate were minimal. Microbial nitrogen (MN) flow to the duodenum and microbial efficiency increased quadratically (P < 0•04) with increased RDP supplementation. Rumen ammonia nitrogen (NH3-N) concentrations increased linearly (P < 0•01) and total volatile fatty acids (VFA) tended to increase linearly (P = 0•07). In conclusion, RDP supplementation to Dohne Merino wethers consuming wheat straw generally enhanced rumen fermentation and forage intake. A total RDP intake (sources: calcium caseinate and wheat straw) of 3•30 g/kg M0•75 or 0•12 of DOM maximized DOMI.

PROTOZOAL CONTRIBUTION TO NITROGEN DIGESTION IN SHEEP

1984 ◽  
Vol 64 (5) ◽  
pp. 20-21 ◽  
Author(s):  
K. USHIDA ◽  
J. P. JOUANY ◽  
BERNADETTE LASSALAS ◽  
P. THIVEND
Keyword(s):  
Key Words ◽  
Dietary Protein ◽  
Microbial Nitrogen ◽  
Ruminal Protein Degradability ◽  
Degradable Protein ◽  
Protein Degradability

Compared with defaunated sheep, the large protozoal population of faunated sheep fed a diet high in poorly degradable protein decreased both microbial and dietary protein flows into the duodenum which indicated that protozoa increased ruminal protein degradability. Protozoal nitrogen represented 20% of the total microbial nitrogen in the duodenum. Key words: Protozoa, microbial proteins, sheep, protein degradability

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