CELL CULTIVATION OF BOVINE RUMEN MUCOSA TISSUES

1984 ◽  
Vol 64 (5) ◽  
pp. 110-111
Author(s):  
S. INOOKA ◽  
S. OHWADA ◽  
H. TAMATE
Keyword(s):  
Cell Culture ◽  
Sodium Chloride ◽  
Key Words ◽  
Sodium Citrate ◽  
Tween 80 ◽  
Cell Cultivation ◽  
Edta Treatment ◽  
Bovine Rumen ◽  
Key Words Cell Culture

This paper describes the separation of rumen mucosa cells by pretrypsinization with 0.1% trypsin elusion solution composed of 2% sodium citrate, 2% EDTA-Na, 0.001% Tween 80 and 0.01% polybrene in 0.9% sodium chloride, pH 7.2. In the use of the cells separated following trypsin-EDTA treatment, three culture cases are described. Key words: Cell culture, rumen mucosa, bovine

Effect of Grinding Aids on the Fracture Energy of Mica

2011 ◽  
Vol 402 ◽  
pp. 503-509
Author(s):  
Ze Hong Wang ◽  
Fu Jia Yu ◽  
Shan Cai ◽  
Shan Zhi Deng ◽  
Roger Horn
Keyword(s):  
Sodium Chloride ◽  
Fracture Energy ◽  
Sodium Citrate ◽  
Ph Value ◽  
Sodium Tripolyphosphate ◽  
Experimental System ◽  
Ammonium Bromide ◽  
Solution Ph ◽  
Grinding Aids ◽  
Slurry Rheology

An extension of the classical Obriemoff experiments has been set up to measure the fracture energy of mica. This experimental system will be entirely independent of slurry rheology. CTAB (Cetyl Trimethyl Ammonium Bromide), sodium tripolyphosphate, sodium hexametaphosphate, and tri-sodium citrate are used as grinding aids in this study and the fracture energy has been measured for mica in air, water, and water with the addition of various concentrations of these grinding aids. The results show that the fracture energy of mica in water is about half of what it is in air. Grinding aids are shown to reduce the fracture energy of mica, but not dramatically. Addition of grinding aids reduces the fracture energy by a further 10-20%, with tri-sodium citrate appearing to be the most effective. For each grinding aid there appears to be an optimal concentration, typically around 10 mmol. An experiment is also done with sodium chloride at a range of concentrations to investigate the mechanism of these grinding aids, but no reduction in fracture energy (compared to water) was observed, hence the molecular-level mechanism of action of these grinding aids remains unclear. The effect of solution pH values on the fracture energy are also investigated using tri-sodium citrate and sodium chloride. The results show that the solution pH value may effect on the fracture energy of mica. So, in practice, both concentration and pH value of solution are important for getting better grinding results.

scholarly journals THE EFFECT OF INORGANIC SALTS ON THE CHEMICAL CHANGES IN THE BLOOD OF THE DOG AFTER OBSTRUCTION OF THE DUODENUM

1924 ◽  
Vol 39 (2) ◽  
pp. 321-330 ◽  
Author(s):  
Russell L. Haden ◽  
Thomas G. Orr
Keyword(s):  
Sodium Chloride ◽  
Inhibitory Action ◽  
Sodium Citrate ◽  
Fatal Outcome ◽  
The Other ◽  
Inorganic Salts ◽  
Untreated Animal ◽  
Protein Nitrogen ◽  
Chemical Changes ◽  
Therapeutic Value

The chemical changes in the blood of dogs treated with various inorganic salts after obstruction of the duodenum are reported. Two dogs treated with sodium chloride survived approximately six times as long as the average untreated animal, one living 22 days, the other 24 days. Ammonium chloride was found to produce an acidosis. The administration of potassium chloride, calcium chloride, and magnesium chloride did not prevent the usual rise in non-protein nitrogen and fall in chlorides, and the fatal outcome. Iodides seemingly hasten the toxic process. Sodium bromide appears to have an inhibitory action upon it, but much less than that of sodium chloride. Sodium sulfate, magnesium sulfate, sodium citrate, monosodium phosphate, and disodium phosphate failed to alter the course of the intoxication. Atropine and pilocarpine were without therapeutic value in preventing the changes characteristic of intestinal obstruction.

scholarly journals FREEZING POINTS OF ANTI-COAGULANT SALT SOLUTIONS

1935 ◽  
Vol 18 (4) ◽  
pp. 485-490 ◽  
Author(s):  
David I. Hitchcock ◽  
Ruth B. Dougan
Keyword(s):  
Sodium Chloride ◽  
Linear Function ◽  
Sodium Citrate ◽  
Freezing Point ◽  
Biological Fluid ◽  
Linear Equations ◽  
Freezing Point Depression ◽  
Salt Solutions ◽  
Freezing Points ◽  
Sodium Chloride Solutions

By a method involving equilibration of ice and solution, and analysis of the solution, freezing point depressions of solutions of sodium citrate, oxalate, and fluoride have been determined over the range Δ = 0.45 to 0.65°C. Determinations with sodium chloride solutions have confirmed the accuracy of the method. In each case the freezing point depression is given, within 0.002°C., as a linear function of the concentration. By the use of these linear equations it is possible to prepare a solution of any of these four salts isotonic with a given biological fluid of known freezing point, provided the latter falls within the range studied.

INDUCTION AND TRANSFER OF THE MICROBIAL CAPACITY TO DEGRADE NITROTOXINS IN THE RUMEN

1984 ◽  
Vol 64 (5) ◽  
pp. 33-34 ◽  
Author(s):  
W. MAJAK ◽  
K.-J. CHENG
Keyword(s):  
Key Words ◽  
Nitrate Reduction ◽  
Bovine Rumen

Nitrocompounds, given intraruminally, were tested as potential inducers of nitrate, nitrite and nitropropanol detoxification in the bovine rumen. Nitroethane supplements stimulated the microbial rate of nitropropanol degradation. Nitrate supplements increased the rates for nitrite and nitrate reduction and this induced capacity was transferred between groups of animals in adjacent pens. Key words: Cattle, nitrate, nitrite, nitropropanol, detoxification

EFFECTS OF A WIDE RANGE OF DIETARY SALT LEVELS ON THE PERFORMANCE OF BROILER CHICKENS

1985 ◽  
Vol 65 (3) ◽  
pp. 773-775 ◽  
Author(s):  
F. G. PROUDFOOT ◽  
H. W. HULAN ◽  
D. M. NASH
Keyword(s):  
Sodium Chloride ◽  
Adverse Effects ◽  
Key Words ◽  
Broiler Chickens ◽  
Dietary Sodium ◽  
Dietary Salt ◽  
Wide Range ◽  
Commercial Broiler ◽  
Dietary Sodium Chloride

Five dietary levels of sodium chloride (0.2, 0.4, 0.6, 1.0 and 1.8%) were used. Commercial broiler chickens tolerated 0.4–1.0% of dietary sodium chloride without adverse effects on performance. Key words: Broilers, salt, diets, sodium chloride

scholarly journals Adsorptive Capacity of Activated Charcoal for Rifampicin with and without Sodium Chloride and Sodium Citrate.

2001 ◽  
Vol 24 (6) ◽  
pp. 724-726 ◽  
Author(s):  
Orish Ebere ORISAKWE ◽  
Onyenmechi Johnson AFONNE ◽  
Patrick U. AGBASI ◽  
Ndidi A. ILONDU ◽  
Sabinus I. OFOEFULE ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Activated Charcoal ◽  
Sodium Citrate ◽  
Adsorptive Capacity

scholarly journals Protein–polysaccharide of chicken cartilage and chondrocyte cell cultures

1970 ◽  
Vol 120 (4) ◽  
pp. 689-697 ◽  
Author(s):  
Herbert J. Shulman ◽  
Karl Meyer
Keyword(s):  
Cell Culture ◽  
Sodium Chloride ◽  
Cell Cultures ◽  
Keratan Sulphate ◽  
Adult Chicken ◽  
Protein Core ◽  
Embryonic Chicken ◽  
The Matrix

The nature of the matrix produced by embryonic chicken chondrocytes in cell culture was studied, and compared with adult and embryonic chicken cartilage. Adult chicken cartilage contains a protein–polysaccharide easily extracted with EDTA–sodium chloride at 4°C. Purification of this macromolecule on Bio-Gel P-300 and Bio-Gel A-50m yielded a progressively more homogeneous species in the ultracentrifuge. It contained mostly chondroitin 4-sulphate, some chondroitin 6-sulphate, and keratan sulphate. Embryonic chicken cartilage was previously shown to contain mostly chondroitin 4-sulphate, some chondroitin 6-sulphate and essentially no keratan sulphate. The matrix produced in chondrocyte cell cultures was shown to contain a protein–polysaccharide with alkali-labile linkages of chondroitin 4-sulphate to the protein core. A fraction was isolated from the matrix with many properties of keratan sulphate.

Activity coefficients of NaCl in NaCl–NaOAc–H2O at 25, 35, and 45 °C

1991 ◽  
Vol 69 (1) ◽  
pp. 111-115 ◽  
Author(s):  
S. Manohar ◽  
J. Ananthaswamy
Keyword(s):  
Sodium Chloride ◽  
Key Words ◽  
Activity Coefficients ◽  
Sodium Acetate ◽  
Osmotic Coefficients ◽  
Free Energies ◽  
Harned's Rule ◽  
Pitzer Formalism ◽  
Scatchard Equation

The activity coefficients of NaCl were estimated by measuring the EMFs of the cell[Formula: see text]at four ionic strengths, i.e., 0.5, 1.0, 2.0, and 3.0 mol/kg and at temperatures 25, 35, and 45 °C. The results were analyzed in terms of Harned's rule, the Pitzer and Rush–Johnson–Scatchard treatments. Osmotic coefficients and excess free energies of mixing were calculated at all ionic strengths and temperatures studied. Key words: activity coefficients, sodium chloride, sodium acetate, Pitzer formalism, Scatchard equation.

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