Modification of muscle inherent properties through age at slaughter, growth promotants and breed crosses

2011 ◽  
Vol 91 (4) ◽  
pp. 635-648 ◽  
Author(s):  
I. Girard ◽  
J. L. Aalhus ◽  
J. A. Basarab ◽  
I. L. Larsen ◽  
H. L. Bruce
Keyword(s):  
Gluteus Medius ◽  
Fibre Types ◽  
Adrenergic Agonist ◽  
Cross Sectional ◽  
Slaughter Age ◽  
Red Angus ◽  
Breed Crosses ◽  
Hormone Use ◽  
Tissue Characteristics ◽  
Growth Promotants

Girard, I., Aalhus, J. L., Basarab, J. A., Larsen, I. L. and Bruce, H. L. 2011. Modification of muscle inherent properties through age at slaughter, growth promotants and breed crosses. Can. J. Anim. Sci. 91: 635–648. A 24 factorial experiment tested the interactions of slaughter age (12–13 or 18–20 mo), growth hormone use, β-adrenergic agonist (β-AA) use and breed cross [Hereford–Aberdeen Angus (HAA) or Charolais–Red Angus (CRA)] on the composition, fibre types, and connective tissue characteristics of m. semitendinosus (ST) and m. gluteus medius (GM) from 112 crossbred steers. Muscle weights increased with slaughter age, implantation and CRA genetics (P<0.05), but were not affected by ractopamine hydrochloride (RAC) (P>0.10).Animal age increased fast glycolytic (FG) and decreased fast oxidative glycolytic (FOG) fibre percentages by 7.2 and 6.6%, respectively, in the ST and increased slow oxidative (SO) and FOG fibre areas in both muscles (P<0.05). Cross-sectional areas of all fibre types were increased in the ST with implantation. In the GM, implantation increased SO (3.1%) and reduced FOG (3.2%) fibre percentages, while RAC reduced the SO (3.8%) and increased the FG (6.1%) fibre percentages (P<0.05).Only GM total collagen content increased with slaughter age (P<0.05),but collagen solubility decreased with slaughter age for both muscles (P<0.05). CRA genetics increased FG percentage in the GM of yearling-fed steers and increased moisture and protein and reduced fat contents of both muscles (P<0.05). In the muscles studied, IMP, slaughter age and animal genetics induced greater changes in muscle inherent properties than RAC.

Modification of beef quality through steer age at slaughter, breed cross and growth promotants

2012 ◽  
Vol 92 (2) ◽  
pp. 175-188 ◽  
Author(s):  
I. Girard ◽  
J. L. Aalhus ◽  
J. A. Basarab ◽  
I. L. Larsen ◽  
H. L. Bruce
Keyword(s):  
Shear Force ◽  
Sarcomere Length ◽  
Gluteus Medius ◽  
Beef Quality ◽  
Water Losses ◽  
Colour Measurements ◽  
Hue Angle ◽  
Red Angus ◽  
Breed Crosses ◽  
Growth Promotants

Girard, I., Aalhus, J. L., Basarab, J. A., Larsen, I. L. and Bruce, H. L. 2012. Modification of beef quality through steer age at slaughter, breed cross and growth promotants. Can. J. Anim. Sci. 92: 175–188. A 23 factorial experiment tested the interactions of slaughter age (12–13 or 18–20 mo), growth implants use (Component E-S, TE-S), ractopamine hydrochloride (RAC) feed supplementation use and breed cross [Hereford–Aberdeen Angus (HAA) or Charolais–Red Angus (CRA)] on pH, temperature, objective colour measurements, relative myoglobin states, sarcomere lengths, shear force, and water losses of m. semitendinosus (ST) and m. gluteus medius (GM) from 112 crossbred steers. In the ST, age affected objective colour measurements by increasing chroma and decreasing lightness (L*) and hue angle (P<0.05). Metmyoglobin (MMB) content of the ST also increased with steer age (P<0.05). In the GM, yearling-fed steers had greater MMB content than calf-fed steers, while hue angle varied the opposite way (P<0.05). Other variations in meat colour and myoglobin contents were more complex in the GM than the ST as they involved three-way interactions between the different treatments. Shear force and purge loss of the ST increased with implantation (P<0.05) with no change in sarcomere length (P>0.05). Shear force standard deviation was similar for breed crosses when yearling-fed but greatest for CRA breed cross when calf-fed (P<0.05). In both muscles, purge loss was increased by RAC supplementation (P<0.05). RAC supplementation did not affect sarcomere length and shear force in both muscles (P>0.10). In the GM, shear force increased with age and with CRA genetics (P<0.05). Results indicated that producers seeking to reduce beef toughness should consider using British crossbreds, exclude the use of hormonal implants and slaughter process steers at 12 to13 mo of age.

scholarly journals Early life undernutrition in rats

1982 ◽  
Vol 47 (3) ◽  
pp. 417-431 ◽  
Author(s):  
K. S. Bedi ◽  
A. R. Birzgalis ◽  
M. Mahon ◽  
J. L. Smart ◽  
A. C. Wareham
Keyword(s):  
Body Weight ◽  
Relative Frequency ◽  
Cross Sectional Area ◽  
Fibre Types ◽  
Male Rats ◽  
Sectional Area ◽  
Muscle Weight ◽  
Cross Sectional ◽  
Volume Proportion ◽  
Fibre Number

1. Male rats were undernourished either during the geslational and suckling periods or for a period of time immediately following weaning. Some rats were killed at the end of the period of undernutrition; others were nutritionally rehabilitated for lengthy periods of time before examination. Two muscles, the extensor digitorum longus (EDL) and soleus (SOL) were studied from each rat. Histochemically-stained transverse sections of these muscles were used to determine total number of fibres, the fibre cross-sectional areas and the relative frequency of the various fibre types.2. All rats killed immediately following undernutrition showed significant deficit sin body-weight, muscle weight and fibre cross-sectional area compared to age-matched controls.3. Animals undernourished during gestation and suckling and then fed normally for 5 months showed persistent and significant deficits in body-weight, muscle weight and total fibre number. There were also significant deficits in mean fibre cross-sectional area of each fibre type except for red fibres in the EDL. No difference in the volume proportion of connective tissue was found.4. Rats undernourished after weaning and then fed ad lib. for approximately 7 months had normal body-and muscle weights. Their muscles showed no significant differences in total fibre number, relative frequency of the various fibre types, fibre size or volume proportion of connective lissue.5. These results indicate that, although the effects on rat skeletal muscle of a period of undernutrition after weaning can be rectified, undernutrition before weaning causes lasting deficits.

scholarly journals Comparison of gluteus medius strength between obese and eutrophic individuals: a cross-sectional study

2020 ◽  
Author(s):  
Rafael Ratti Fenato ◽  
Allan Cezar Faria Araujo ◽  
Ana Tereza Bittencourt Guimarães
Keyword(s):  
Principal Component ◽  
Gluteus Medius ◽  
Great Difficulty ◽  
Cross Sectional Study ◽  
Normal Weight ◽  
Cross Sectional ◽  
Human Research Ethics ◽  
Hip Abductor ◽  
Lateral Decubitus ◽  
Muscular Function

Abstract Background: The hip abductor muscles, primarily the gluteus medius, play an important role in stabilizing the pelvis during gait. Gluteus medius weakness is associated with biomechanical changes and musculoskeletal disorders. Obese individuals can have great difficulty maintaining abductor muscular function due to being overweight and possibly experiencing a decrease in muscle mass. However, it is still unclear whether the musculature of obese individuals can compensate for these changes. Therefore, the aim of this study was to compare gluteus medius strength between obese and normal-weight individuals using a digital hand-held dynamometer.Methods: Twenty-five obese (BMI > 35 kg/m2) participants were matched for sex, age, and height with normal-weight individuals. Gluteus medius strength was measured by a single examiner using a belt-stabilized hand-held digital dynamometer placed on the knee of the individuals positioned in lateral decubitus. Three measurements were recorded with rest intervals, and only the highest value measured for each limb was used for analysis. The differences between pairs were calculated, and the normality of the data was assessed using the Shapiro-Wilk test (p < 0.05). The matrices of the variables were standardized and analysed using principal component analysis (PCA).Results: For the strength variables (Newtons) on both sides, no significant differences were detected between the groups (p > 0.05). However, significant differences were detected in these variables between the groups (p < 0.05) when the measurements were normalized to body weight (Newtons/kilograms). PCA indicated that both the absolute and normalized values of strength are lower in obese than in normal-weight individuals.Conclusions: These findings indicate that obese individuals have the same or less strength (PCA) to move more mass, which may suggest a relative weakness that induces functional limitations.Trial registration: The study was approved by the UNIOESTE Human Research Ethics Committee (#1.180.202) in July 2015.

scholarly journals Effects of hormonal growth promotants (HGP) on growth, carcass characteristics, the palatability of different muscles in the beef carcass and their interaction with aging

2008 ◽  
Vol 48 (11) ◽  
pp. 1405 ◽  
Author(s):  
J. M. Thompson ◽  
B. M. McIntyre ◽  
G. D. Tudor ◽  
D. W. Pethick ◽  
R. Polkinghorne ◽  
...  
Keyword(s):  
Carcass Characteristics ◽  
Gluteus Medius ◽  
Biceps Femoris ◽  
Sensory Testing ◽  
Fat Percentage ◽  
Triceps Brachii ◽  
Trenbolone Acetate ◽  
Aging Rates ◽  
Growth Promotants ◽  
Feed Samples

Effects of hormonal growth promotant (HGP) implantation on liveweight, carcass and meat quality measurements were examined using 80 Angus yearling cattle. After entry to the feedlot, 40 steers and 40 heifers were implanted with Revalor-S (28 mg oestradiol and 140 mg trenbolone acetate) and Revalor-H (20 mg oestradiol, 200 mg trenbolone acetate), respectively. Cattle were slaughtered after 55 and 65 days on feed. Samples from the Mm. longissimus dorsi, biceps femoris (the cap and body portions), gluteus medius (the eye and D portions), infraspinatus and triceps brachii were prepared for sensory testing after aging for 5 and 21 days after slaughter. A total of 854 muscle samples were cooked by grill (601) or roast (253) methods and served to consumers using the Meat Standards Australia taste panel protocols. When adjusted to the same initial liveweight, implantation with Revalor-H and Revalor-S resulted in a 4 and 7% increase in slaughter weight, respectively. Implantation resulted in an increased ossification score in steers (P < 0.05), but not in heifers. There was a significant interaction (P < 0.05) between HGP implantation and days aged for shear force. There was a small effect of HGP implants on compression (P < 0.05), but not on cook loss and intramuscular fat percentage. Muscles differed in their response to HGP implantation (P < 0.05) for tenderness, overall liking and palatability scores. Muscles also differed in their aging rates after slaughter (P < 0.05). The greatest response in sensory scores to HGP implantation was found in those muscles that had the highest aging rates. Possible mechanisms by which muscles differed in their response to HGP implantation are discussed.

Rat diaphragm during postnatal development. I. Changes in distribution of muscle fibre type and in oxidative potential

1996 ◽  
Vol 8 (3) ◽  
pp. 391 ◽  
Author(s):  
MD Fratacci ◽  
M Levame ◽  
A Rauss ◽  
H Bousbaa ◽  
G Atlan
Keyword(s):  
Muscle Fibre ◽  
Fibre Type ◽  
Oxidative Capacity ◽  
Cross Sectional Area ◽  
Fibre Types ◽  
Type I ◽  
Sectional Area ◽  
Rat Diaphragm ◽  
Oxidative Potential ◽  
Cross Sectional

The changes occurring in the histochemical characteristics of the rat diaphragm during the postnatal period were examined. Fibre-type distribution, fibre oxidative capacity, i.e. succinate-dehydrogenase (SDH) activity, and cross-sectional area were compared in the costal (COS) and crural (CRU) regions, and across their abdominal and thoracic surfaces. The proportions of type I and IIb fibres in both COS and CRU increased with age, while the proportion of type IIa fibres progressively decreased. For COS, fibre distribution was homogeneous over the entire muscle and did not change after 4 weeks. For CRU, it was heterogeneous with a higher proportion of type I fibres on the thoracic surface as from the first week. All fibre types significantly increased in cross-sectional area between 1 and 8 weeks, with no significant differences in COS and CRU. Mean SDH activity did not differ between COS and CRU or across the muscles. Mean SDH activities-were low and identical in all fibre types at birth, and then increased, peaking at the 6th week in type I and IIa fibres. When total muscle fibre oxidative capacity was calculated from an index including fibre-type proportion, cross-sectional area and mean SDH activity, it was significantly higher at 1 than at 8 weeks after birth; this might have functional implications for the newborn.

Electromyography Activation Levels of the 3 Gluteus Medius Subdivisions During Manual Strength Testing

2015 ◽  
Vol 24 (3) ◽  
pp. 244-251
Author(s):  
Roald Otten ◽  
Johannes L. Tol ◽  
Per Holmich ◽  
Rodney Whiteley
Keyword(s):  
Study Design ◽  
Repeated Measures ◽  
Gluteus Medius ◽  
Strength Testing ◽  
Cross Sectional ◽  
Physically Active ◽  
Rehabilitation Programs ◽  
Significant Difference ◽  
The Individual ◽  
Hip Internal Rotation

Study Design:Cross-sectional.Context:Gluteus medius (GM) muscle dysfunction is associated with overuse injury. The GM is functionally composed of 3 separate subdivisions: anterior, middle, and posterior. Clinical assessment of the GM subdivisions is relevant to detect strength and activation deficits and guide specific rehabilitation programs. However, the optimal positions for assessing the strength and activation of these subdivisions are unknown.Objective:The first aim was to establish which strength-testing positions produce the highest surface electromyography (sEMG) activation levels of the individual GM subdivisions. The second aim was to evaluate differences in sEMG activation levels between the tested and contralateral (stabilizing) leg.Method:Twenty healthy physically active male subjects participated in this study. Muscle activity using sEMG was recorded for the GM subdivisions in 8 different strength-testing positions and analyzed using repeated-measures analysis of variance.Results:Significant differences between testing positions for all 3 GM subdivisions were found. There were significant differences between the tested and the contralateral anterior and middle GM subdivisions (P < .01). The posterior GM subdivision showed no significant difference (P = .154).Conclusion:Side-lying in neutral and side-lying with hip internal rotation are the 2 positions recommended to evaluate GM function and guide specific GM rehabilitation.

Fibre types in breast and leg muscles of hand-reared and wild grey partridge (Perdix perdix)

1998 ◽  
Vol 76 (2) ◽  
pp. 236-242 ◽  
Author(s):  
Ahti EI Pyörnilä ◽  
Ahti P Putaala ◽  
Raimo K Hissa
Keyword(s):  
Relative Number ◽  
Fibre Types ◽  
Muscle Fibres ◽  
Poor Survival ◽  
Cross Sectional ◽  
Perdix Perdix ◽  
Grey Partridge ◽  
The Poor ◽  
In The Wild ◽  
Fast Twitch

Fibre types and sizes and their relative numbers and cross-sectional areas in M. pectoralis, M. supracoracoideus, and M. iliotibialis of hand-reared and wild grey partridge (Perdix perdix) were determined in order to see if there are differences that could account for the poor survival of hand-reared birds released into the wild. Histochemical staining for myosin ATPase and succinate dehydrogenase (SDH) showed that most breast-muscle fibres (80-90%) are of the fast-twitch glycolytic (FG) type and a smaller portion of the fast-twitch oxidative glycolytic (FOG) type. In M. iliotibialis, about 60% of the fibres were FG fibres and the rest were of the FOG type. Judging from the low intensity of SDH staining, FOG fibres in the grey partridge appear weakly oxidative only. The relative number of FG fibres and their relative cross-sectional area in M. pectoralis were larger in the hand-reared than in the wild birds. The cross-sectional areas of both fibre types in M. iliotibialis were significantly larger in the hand-reared birds. Taken as a whole, these findings alone do not account for the poor survival of hand-reared partridge in the wild.

Effects of calf- and yearling-fed beef production systems and growth promotants on production and profitability

2013 ◽  
Vol 93 (1) ◽  
pp. 171-184 ◽  
Author(s):  
Óscar López-Campos ◽  
Jennifer L. Aalhus ◽  
Erasmus K. Okine ◽  
Vern S. Baron ◽  
John A. Basarab
Keyword(s):  
Dry Matter ◽  
Production Systems ◽  
Beef Production ◽  
Feed Conversion ◽  
Adrenergic Agonist ◽  
Growth Implants ◽  
Quality Grade ◽  
Reduce Risk ◽  
Growth Promotants ◽  
Growth Implant

López-Campos, Ó., Aalhus, J. L., Okine, E. K., Baron, V. S. and Basarab, J. A. 2013. Effects of calf- and yearling-fed beef production systems and growth promotants on production and profitability. Can. J. Anim. Sci. 93: 171–184. In each of 2 yr, 112 spring-born steers were used to evaluate the effect of calf-fed vs. yearling-fed with and without growth implant and β-adrenergic agonist on production parameters and economic potential. Steers were grouped into: (1) non-implanted feeders harvested at 11–14 mo of age, (2) growth implanted feeders harvested at 11–14 mo of age, (3) non-implanted feeders harvested at 19–23 mo of age, and (4) growth implanted feeders harvested at 19–23 mo of age. Production data were collected and economic evaluation was performed. Calf-fed steers grew slower (1.21 vs. 1.99±0.07 kg d−1) and had a poorer feed conversion ratio [5.32 vs. 4.99±0.34 kg dry matter intake (DMI) kg−1 gain] during the feedlot dietary adjustment period than yearling-fed. Calf-fed steers were more efficient than yearling-fed during the first 76–83 d (5.16 vs. 7.33±0.11 kg DMI kg−1 gain) and latter 48–79 d (5.69 vs. 14.28±1.50 kg DMI kg−1 gain) of the finishing period. Implanted steers were more efficient than non-implanted during the dietary feedlot adjustment period (4.80 vs. 5.52±0.15 kg DMI kg−1 gain), and during the first 76–83 d (6.05 vs. 6.44±0.11 kg DMI kg−1 gain) and latter 48–79 d of the finishing period (9.29 vs. 10.69±1.50 kg DMI kg−1 gain). Implanted steers grew 11.4–19.6% faster than non-implanted throughout the finishing period, while yearling-fed grew 11.1–12.9% faster during the first 76–83 d, but 49.1–64.4% slower during the last 48–79 d of the finishing period compared with calf-fed. Quality grade was improved for non-implanted steers, with 43.6% of yearling-fed and 35.7% calf-fed steers grading AAA. Adjusted net return was best for calf-fed implanted ($17.52 head−1), followed by calf-fed non-implanted ($−41.92 head−1), yearling-fed implanted ($−73.77 head−1), and yearling-fed non-implanted ($−99.65 head−1) production strategies. The results of the present study suggest that reducing age at slaughter combined with growth implant can increase profit and reduce risk, but growth implants can negatively affect the carcass quality.

scholarly journals Histochemical and Morphometric Evaluation of Skeletal Muscle from Horses with Exertional Rhabdomyolysis (Tying-up)

1986 ◽  
Vol 23 (4) ◽  
pp. 400-410 ◽  
Author(s):  
S. A. McEwen ◽  
T. J. Hulland
Keyword(s):  
Skeletal Muscle ◽  
Clinical Signs ◽  
Gluteus Medius ◽  
Control Group ◽  
Type Ii ◽  
Cross Sectional ◽  
Gluteus Medius Muscle ◽  
Exertional Rhabdomyolysis ◽  
Exercise Period ◽  
Type Ii Fibers

Thirteen horses with histories of exertional rhabdomyolysis were exercised for 20 minutes to induce clinical signs of lameness, elevated serum creatine kinase (CK), and aspartate aminotransferase (AST) activities and skeletal muscle morphologic lesions. The clinical signs exhibited by affected horses included trembling, sweating, increased rate of respiration, and restricted limb movement. Serum CK reached maximal activity between 4 and 8 hours after the exercise period and serum AST activity peaked between 24 and 48 hours. Histologically, the skeletal muscle lesions in muscle biopsies 24 hours after the exercise period consisted of segmental muscle fiber degeneration. Damaged muscle fibers were repaired by myoblastic regeneration. Horses with moderate (>1,500 U/liter) to severe (>5,000 U/liter) elevations of serum CK activity accompanied by clinical signs of muscle soreness induced by exercise, had visible muscle fiber degeneration microscopically. Frozen sections of biopsies of the gluteus medius muscle from affected ( n = 13) and control ( n = 11) groups of horses were processed to demonstrate myofibrillar ATPase activity. These sections were then used to determine fiber types, area percentages, and mean cross sectional fiber sizes. The mean type I, type II, and intermediate fiber sizes were significantly larger in the affected group than in the control group. In the gluteus medius muscles of the affected group, there was a significantly greater percentage of intermediate fibers and a significantly greater percentage of area occupied by intermediate fibers than in the control group. In the muscle samples with acute lesions of exertional rhabdomyolysis, type II fibers were selectively but not exclusively affected. In one horse which was subsequently necropsied 24 hours after the exercise period, lesions were present in several postural muscles, the masseter muscle and the heart. We conclude that the gluteus medius muscle fibers of affected horses are larger in cross sectional area than those of control horses and that there is preferential degeneration of type II fibers in acute lesions of exertional rhabdomyolysis.

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