scholarly journals Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells

2009 ◽  
Vol 53 (2) ◽  
pp. 10 ◽  
Author(s):  
YH Huang ◽  
MJ Sun ◽  
M Jiang ◽  
BY Fu
Keyword(s):  
Pancreatic Polypeptide ◽  
Endocrine Pancreas ◽  
Islet Cells ◽  
Immunohistochemical Localization

Immunohistochemical localization of somatostatin and pancreatic polypeptide in smokers with chronic pancreatitis

2012 ◽  
Vol 114 (5) ◽  
pp. 495-502 ◽  
Author(s):  
Mariola Sliwinska-Mosson ◽  
Halina Milnerowicz ◽  
Stanislaw Milnerowicz ◽  
Marcin Nowak ◽  
Jerzy Rabczynski
Keyword(s):  
Chronic Pancreatitis ◽  
Pancreatic Polypeptide ◽  
Immunohistochemical Localization

scholarly journals Immunohistochemistry of Pancreatic Islet Cell Tumors in the Ferret (Mustela putorius furo)

1997 ◽  
Vol 34 (5) ◽  
pp. 387-393 ◽  
Author(s):  
G. A. Andrews ◽  
N. C. Myers ◽  
C. Chard-Bergstrom
Keyword(s):  
Pancreatic Islets ◽  
Pancreatic Islet ◽  
Pancreatic Polypeptide ◽  
Islet Cell ◽  
Islet Cells ◽  
Neuron Specific Enolase ◽  
Islet Cell Tumors ◽  
Pancreatic Islet Cell ◽  
Formalin Fixed Paraffin Embedded ◽  
Pancreatic Islet Cell Tumors

Twenty-two pancreatic islet cell tumors and normal pancreatic islets from ferrets were evaluated by immunohistochemistry for expression of the peptide hormones insulin, somatostatin, glucagon, and pancreatic polypeptide (PP) and the neuroendocrine markers chromogranin A (CgA) and neuron-specific enolase (NSE). In normal pancreatic islets, the majority of cells stained strongly with CgA and NSE. A cells, B cells, D cells, and PP cells stained strongly with glucagon, insulin, somatostatin, and PP, respectively. All 22 tumors stained with CgA and NSE. The proportion of cells within tumors staining for CgA was variable, but more than half of the cells stained positively in 18 of the tumors. The intensity of staining for CgA was strong (reactivity equivalent to or greater than normal islet cells in adjacent tissue) in 11 moderate in six, and weak in five of the tumors. All tumors stained for NSE, with ≥50% of the cells staining in 21 of the tumors, and the intensity of staining was strong in 18 of the tumors. Twenty of 22 tumors stained positively for insulin, with ≥50% of the cells staining in 19 of them. The intensity of staining for insulin was strong in 12, moderate in seven, and weak in one of the tumors. Approximately ≤1% of the cells in 15 of 22 tumors stained for somatostatin, five tumors stained for pancreatic polypeptide, and three tumors stained for glucagon. These data indicate that the majority of islet cell tumors of ferrets express immunohistochemically detectable insulin. CgA and NSE are both useful general markers for such tumors, including those that are insulin negative. Commercially available antisera to CgA, NSE, insulin, glucagon, somatostatin, and PP work well in formalin-fixed, paraffin-embedded tissue for immunophenotyping islet cell tumors in the ferret.

Effects of somatostatin and pancreatic polypeptide on exocrine and endocrine pancreas in the rats

1988 ◽  
Vol 23 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Wontae Lee ◽  
Kazunori Miyazaki ◽  
Akihiro Funakoshi
Keyword(s):  
Pancreatic Polypeptide ◽  
Endocrine Pancreas

scholarly journals QUANTITATIVE STUDIES ON ISOLATED PANCREATIC ISLETS OF MAMMALS: ADENOSINE TRIPHOSPHATASE ACTIVITY IN NORMAL AND OBESE-HYPERGLYCEMIC MICE

1964 ◽  
Vol 12 (7) ◽  
pp. 491-497 ◽  
Author(s):  
INGE-BERT TÄLJEDAL ◽  
BO HELLMAN ◽  
CLAES HELLERSTRÖM
Keyword(s):  
Enzyme Activity ◽  
Adenosine Triphosphatase ◽  
Endocrine Pancreas ◽  
Islet Cells ◽  
Adenosine Diphosphate ◽  
Histochemical Staining ◽  
Isolated Pancreatic Islets ◽  
Quantitative Studies ◽  
Substrate Cleavage ◽  
Substrate Medium

Microchemical and histochemical methods were used for the characterization, localization and assay of adenosine triphosphate (ATP) splitting enzymes in homogenates and sections of the endocrine pancreas from obese-hyperglycemic mice and their lean litter mates. The following observations were made: 1. Dephosphorylation of ATP was maximal at pH 9.1. It was strongly stimulated by magnesium ions at an optimal concentration of 1 mM. ATP cleavage was inhibited by adenosine diphosphate, sodium azide and p-chloromercuribenzoic acid. The addition of l-cysteine, sodium cyanide or sodium fluoride to the substrate medium had no effect on the enzyme activity. Substitution of ATP by equimolar amounts of other phosphate esters in the medium considerably reduced the substrate cleavage. These results are taken as evidence for the presence of sulfhydryl-dependent adenosine triphosphatase (ATPase) in the islet tissue. 2. Histochemical staining revealed a strong ATP splitting enzyme activity in the capillaries and walls of larger blood vessels throughout the pancreas; a rather weak and diffuse cytoplasmic reaction being found in the islet cells. 3. Microchemical assays revealed a lower cleavage of ATP in the islets as compared with the exocrine pancreas and the liver. The cleavage of ATP was more intense in the islets of the obese-hyperglycemic mice than in those of the lean litter mates. 4. Starvation for 7 days induced no significant changes in the enzyme activity of the endocrine pancreas.

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