Immunohistochemical localization of human pancreatic polypeptide (HPP) to a population of islet cells

1975 ◽  
Vol 156 (2) ◽  
Author(s):  
L.-I. Larsson ◽  
F. Sundler ◽  
R. H�kanson
Keyword(s):  
Pancreatic Polypeptide ◽  
Islet Cells ◽  
Immunohistochemical Localization ◽  
Human Pancreatic Polypeptide

scholarly journals Malignant Pancreatic Polypeptide Secreting Tumour of Islet Cells: A Case for Aggressive Surgical Palliation

HPB Surgery ◽  
1993 ◽  
Vol 6 (4) ◽  
pp. 301-309 ◽  
Author(s):  
R. D. Pullan ◽  
M. W. Scriven ◽  
J. O'dowd ◽  
A. T. Edwards ◽  
M. H. Lewis
Keyword(s):  
Pancreatic Polypeptide ◽  
Severe Pain ◽  
Islet Cells ◽  
Locally Advanced ◽  
Palliative Surgery ◽  
Term Survival ◽  
Aggressive Management ◽  
Pancreatic Duct Obstruction ◽  
Human Pancreatic Polypeptide

A case of a malignant pancreatic polypeptide secreting tumour is reported. The tumour was metastatic at presentation at which time it was excised. Pancreatic duct obstruction occurred 3 years after excision causing severe pain on eating. Major palliative surgery, in the form of a pancreatico-jejunostomy, cured the severe symptoms. The patient survives, largely symptom free, over six years after original excision. This case illustrates the need for aggressive management of symptoms in tumours in which long term survival is possible despite locally advanced or metastatic disease.ABBREVIATIONS: VIP — vasoactive intestinal peptide. CT — computed tomography. GI — gastrointestinal. HPP — human pancreatic polypeptide. APUD — amine precursor uptake and decarboxylation.

scholarly journals Structure of a precursor to human pancreatic polypeptide.

1984 ◽  
Vol 259 (23) ◽  
pp. 14702-14705 ◽  
Author(s):  
A B Leiter ◽  
H T Keutmann ◽  
R H Goodman
Keyword(s):  
Pancreatic Polypeptide ◽  
Human Pancreatic Polypeptide

Immunohistochemical localization of somatostatin and pancreatic polypeptide in smokers with chronic pancreatitis

2012 ◽  
Vol 114 (5) ◽  
pp. 495-502 ◽  
Author(s):  
Mariola Sliwinska-Mosson ◽  
Halina Milnerowicz ◽  
Stanislaw Milnerowicz ◽  
Marcin Nowak ◽  
Jerzy Rabczynski
Keyword(s):  
Chronic Pancreatitis ◽  
Pancreatic Polypeptide ◽  
Immunohistochemical Localization

scholarly journals Immunohistochemistry of Pancreatic Islet Cell Tumors in the Ferret (Mustela putorius furo)

1997 ◽  
Vol 34 (5) ◽  
pp. 387-393 ◽  
Author(s):  
G. A. Andrews ◽  
N. C. Myers ◽  
C. Chard-Bergstrom
Keyword(s):  
Pancreatic Islets ◽  
Pancreatic Islet ◽  
Pancreatic Polypeptide ◽  
Islet Cell ◽  
Islet Cells ◽  
Neuron Specific Enolase ◽  
Islet Cell Tumors ◽  
Pancreatic Islet Cell ◽  
Formalin Fixed Paraffin Embedded ◽  
Pancreatic Islet Cell Tumors

Twenty-two pancreatic islet cell tumors and normal pancreatic islets from ferrets were evaluated by immunohistochemistry for expression of the peptide hormones insulin, somatostatin, glucagon, and pancreatic polypeptide (PP) and the neuroendocrine markers chromogranin A (CgA) and neuron-specific enolase (NSE). In normal pancreatic islets, the majority of cells stained strongly with CgA and NSE. A cells, B cells, D cells, and PP cells stained strongly with glucagon, insulin, somatostatin, and PP, respectively. All 22 tumors stained with CgA and NSE. The proportion of cells within tumors staining for CgA was variable, but more than half of the cells stained positively in 18 of the tumors. The intensity of staining for CgA was strong (reactivity equivalent to or greater than normal islet cells in adjacent tissue) in 11 moderate in six, and weak in five of the tumors. All tumors stained for NSE, with ≥50% of the cells staining in 21 of the tumors, and the intensity of staining was strong in 18 of the tumors. Twenty of 22 tumors stained positively for insulin, with ≥50% of the cells staining in 19 of them. The intensity of staining for insulin was strong in 12, moderate in seven, and weak in one of the tumors. Approximately ≤1% of the cells in 15 of 22 tumors stained for somatostatin, five tumors stained for pancreatic polypeptide, and three tumors stained for glucagon. These data indicate that the majority of islet cell tumors of ferrets express immunohistochemically detectable insulin. CgA and NSE are both useful general markers for such tumors, including those that are insulin negative. Commercially available antisera to CgA, NSE, insulin, glucagon, somatostatin, and PP work well in formalin-fixed, paraffin-embedded tissue for immunophenotyping islet cell tumors in the ferret.

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