Assessment of Cytotoxic Activity of Agave cantula Using Brine Shrimp (Artemia salina) Lethality Bioassay

2010 ◽  
Vol 4 (1) ◽  
pp. 90-94 ◽  
Author(s):  
S. Ramachandr ◽  
M. Vamsikrish ◽  
K.V. Gowthami ◽  
B. Heera ◽  
M.D. Dhanaraju
Keyword(s):  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Artemia Salina

scholarly journals UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2019 ◽  
Vol 7 (3) ◽  
pp. 265
Author(s):  
Faradila Y. Karim ◽  
Nickson J Kawung ◽  
Billy Th. Wagey
Keyword(s):  
Cytotoxic Activity ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Bioactive Compound ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Thalassia Hemprichii ◽  
Brine Shrimp Lethality ◽  
A Value ◽  
Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)  

scholarly journals Skrining Fitokimia dan Uji Aktivitas Sitotoksik dari Tumbuhan Sarang Semut (Myrmecodia beccarii Hook.f.) Asal Kabupaten Merauke

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Modustriati P. Mardany ◽  
Linus Y. Chrystomo ◽  
Aditya K. Karim
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Medicinal Plant ◽  
Cytotoxic Activity ◽  
Active Compound ◽  
Brine Shrimp ◽  
Ethanolic Extract ◽  
Artemia Salina ◽  
Test Method ◽  
High Cytotoxic Activity

Sarang semut (Myrmecodia beccarii Hook.f.) is a famous medicinal plant of Papua, which has traditionally potential in treating a wide variety of mild and severe illnesses, such as cancers and tumors, gout, coronary heart disease, hemorrhoids, tuberculosis, migraines, rheumatism, and leukemia. The purposes of this study were to determine the content of the active compound group and the cytotoxic activity of sarang semut plants from Merauke Region. The extraction was done by maceration using 96 % ethanol. The concentration of ethanolic extract were 0, 25, 50, 75, 100 ppm. The results of the study showed that the cytotoxic activity determine using BSLT (Brine Shrimp Lethality Test) method have a high cytotoxic activity with LC50 values of 22.86 ppm and containing the active compound namely flavonoids, tannins and saponins separately. The ethanolic extract of tuber of sarang semut (M. beccarii) has a high cytotoxic activity against larvae of Artemia salina Leach according BSLT method. Key words: Active compound, M. beccarii, cytotoxic activity, Merauke.

scholarly journals A Cytotoxic Activity of Tahongai (Kleinhovia hospita Linn.) Leaves Extracts Using Brine Shrimp Lethality Test

2019 ◽  
Vol 4 (3) ◽  
pp. 60
Author(s):  
Indah Solihah ◽  
Herlina Herlina ◽  
Riana Sari Puspita Rasyid ◽  
Tri Suciati ◽  
Khairunnisa Khairunnisa
Keyword(s):  
Statistical Analysis ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Ethanol Extracts

Tahongai (Kleinhovia hospita Linn.) leaves had been known contain alkaloids, flavonoids, saponins, steroids, and tannins. At Komering, South Sumatera tribe, tahongai leaves had been known to treat tumor, cancer, polyps, acne, and dysmenorrhea. The study of cytotoxic activity of tahongai bark and stem were done. This study aims to determine the cytotoxic activity of tahongai leaves extracts using BSLT method. Tahongai leaves were extracted using gradual maceration with n-hexane, ethyl acetate, and ethanol 96%. Each extract was tested cytotoxic activity towards Artemia salina L. larvae. The yield of n-hexane, ethyl acetate, and ethanol extracts are 2,686%, 7,033%, and 7,933% respectively. Ethanol extract of tahongai leaves had the best cytotoxic activity with lethality value 76,667% at 500ppm. Statistical analysis with two way ANOVA showed extract and concentration had a significant (p<0,05) effect on larvae lethality percentage.

scholarly journals Antibacterial and Cytotoxic Activity of Longiverbenone Isolated from the Rhizome of Cyperus scariosu

1970 ◽  
Vol 25 (1) ◽  
pp. 82-84 ◽  
Author(s):  
M Shafiqur Rahman ◽  
M Nural Anwar
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Artemia Salina ◽  
Diffusion Method ◽  
Chromatographic Technique

Longiverbenone is a naturally occurring sesquiterpene isolated from ethanolic extract of Cyperus scariosus rhizome by solvent-solvent portioning and chromatographic technique. The antibacterial activity of longiverbenone was evaluated against eleven potential human pathogenic bacteria using disc diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth macrodilution method. Cytotoxic activity (lethal concentration 50%, LC50) of longiverbenone was determined on new borne brine shrimp (Artemia salina). Longiverbenone showed moderate to good antibacterial activity against the test organisms tested herein. It exhibited the lowest MIC (20 μg/ml) and MBC (80 μg/ml) against Vibrio cholerae. The LC50 of the isolated sesquiterpene was found to be 14.38 μg/ml against new borne brine shrimp. Keywords: Antibacterial activity; Cytotoxic activity (LC50); Cyperus scariosu; Longiverbenone; Minimum inhibitory concentration (MIC)DOI: http://dx.doi.org/10.3329/bjm.v25i1.4866 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 82-84

scholarly journals Cytotoxic Activity of Orsellinates

2006 ◽  
Vol 61 (9-10) ◽  
pp. 653-657 ◽  
Author(s):  
Alcir T. Gomes ◽  
Neli K. Honda ◽  
Fernanda M. Roese ◽  
Rozanna M. Muzzi ◽  
Leandro Sauer
Keyword(s):  
Cytotoxic Activity ◽  
Natural Product ◽  
Hydroxy Group ◽  
Brine Shrimp ◽  
Linear Chain ◽  
Artemia Salina ◽  
Chain Elongation ◽  
Log P ◽  
Brine Shrimp Lethality ◽  
Parmotrema Tinctorum

AbstractThe series of 2,4-dihydroxy-6-methylbenzoates 2-10 (methyl to hexyl orsellinates) prepared by alcoholysis of lecanoric acid (1) - a natural product from the lichen Parmotrema tinctorum (Nyl.) Hale - was submitted to the brine shrimp lethality test (BST), which was also performed for 2,4-dihydroxy-6-methylbenzoic acid (11) (orsellinic acid) and the derivative ethyl-2-hydroxy-4-methoxy-6-methylbenzoate (12) (4-methoxy-ethyl orsellinate), in order to detect new substances with probable antineoplasic activity. Results showed that chain elongation - increase in lipophilicity (log P) - causes a rise in the cytotoxic activity of orsellinates. Hexyl orsellinate (7) showed the highest cytotoxic activity (LC50 = 31 μм). A correlation between lipophilicity (log P) and cytotoxic activity (log 1/LC50) is presented. Compounds with ramified chains - iso-propyl, sec-butyl and tert-butyl orsellinates (8-10) - were less active than those with the correspondent linear chain. The activities presented by 4-methoxy-ethyl orsellinate (12) and ethyl orsellinate (3) suggest that the hydroxy group at the C-4 position causes effect in the cytotoxic activity of orsellinates against Artemia salina.

scholarly journals UJI AKTIVITAS SITOTOKSIK EKSTRAK METANOL BUAH KETUMBAR (Coriandrum sativum Linn) TERHADAP ARTEMIA SALINA LEACH DENGAN UJI BSLT (Brine Shrimp Lethality Test)

2020 ◽  
Vol 2 (3) ◽  
pp. 137-146
Author(s):  
Mega Yulia ◽  
Rani Anggraini ◽  
Farizal Farizal
Keyword(s):  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Coriandrum Sativum ◽  
Brine Shrimp Lethality ◽  
Activity Test ◽  
Anti Cancer ◽  
Expected Benefits

Coriander is a common herb can be used as traditional medicine. One of the expected benefits of Coriander fruits is an anti-cancer. This study aims to determine the cytotoxic activity of Coriander extract (Coriandrum sativum Linn) on shrimp larvae (Artemia salina Leach) with the BSLT (Brine Shrimp Lethality Test) method. Coriander fruits was extracted using methanol solvent and maceration method, then concentrated it with a rotary evaporator until a thick extract. Cytotoxic activity test used methanol extract with several concentration : 1,000 ppm, 100 ppm, 10 ppm, and 1 ppm. The result of this research reveal that methanol extract of Coriander fruits has cytotoxic activity with LC50 value is 32.35 ppm.

scholarly journals Cytotoxicity of antimalarial plant extracts from Kenyan biodiversity to the brine shrimp, Artemia salina L. (Artemiidae)

2012 ◽  
Vol 2 (1) ◽  
pp. 12 ◽  
Author(s):  
Joseph Mwanzia Nguta ◽  
James Mucunu Mbaria ◽  
Daniel Waweru Gakuya ◽  
Peter Karuri Gathumbi ◽  
John David Kabasa ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Plant Extracts ◽  
Anticancer Agents ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Aqueous Extracts ◽  
Crude Extracts ◽  
Organic Extracts ◽  
Alternative Test ◽  
Positive Controls

<em>Artemia salina</em> (Artemiidae), the brine shrimp larva, is an invertebrate used in the alternative test to determine toxicity of chemicals and natural products. In this study the medium lethal concentration fifty (LC50 values) of 45 antimalarial plant extracts and positive controls, cyclophosphamide and etoposide were determined using <em>Artemia salina</em> (Artemiidae). Out of the 45 organic extracts screened for activity against <em>Artemia salina </em>larvae, 23 (51%) of the crude extracts demonstrated activity at or below 100 μg/mL, and were categorized as having strong cytotoxic activity, 18 (40%) of the crude extracts had LC50 values between 100 μg/mL and 500 μg/mL, and were categorized as having moderate cytotoxicity, 2 (4.5%) of the crude extracts had LC50 values between 500 μg/mL and 1000 μg/mL, and were considered to have weak cytotoxic activity, while 2 (4.5%) of the crude extracts had LC50 values greater than 1000 μg/mL and were considered to be non toxic. Approximately 20% (9) of the aqueous extracts demonstrated activity at or below 100 g/mL and were considered to have strong cytotoxic activity, 40% (18) of the screened aqueous crude extracts had LC50 values between 100 μg/mL and 500 μg/mL and were considered to be moderately cytotoxic, 16% (7) of the crude extracts had LC50 values between 500 μg/mL and 1000 μg/mL and were considered to have weak cytotoxic activity while 24% (11) of the aqueous extracts had LC50 values greater than 1000μg/mL and were categorized as non toxic The positive controls, cyclophosphamide and etoposide exhibited strong cytotoxicity with LC50 values of 95 μg/mL and 6 μg/mL respectively in a 24 hour lethality study, validating their use as anticancer agents. In the current study, 95.5% of all the screened organic extracts and 76% of the investigated aqueous extracts demonstrated LC50 values &lt;1000 g/mL, indicating that these plants could not make safe anti-malarial treatments. This calls for dose adjustment amongst the community using the plant extracts for the treatment of malaria and chemical investigation for isolation of bioactive compounds responsible for the observed toxicity.

scholarly journals THE CYTOTOXIC ACTIVITY OF N-HEXANE EXTRACT OF KERSEN(muntingia calabura linn.) LEAVES USING THE BRINE SHRIMP LETHALITY TEST (BSLT) METHOD

2016 ◽  
Vol 16 (2) ◽  
pp. 37-44
Author(s):  
Irma Sari ◽  
Titania Miranda ◽  
Sadli Sadli
Keyword(s):  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Hexane Extract ◽  
Water Soluble ◽  
Extractive Content ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Muntingia Calabura

The cytotoxic activity experiment of n-hexane extract of kersen (Muntingia calabura Linn.) leaves has been carried out using the Brine Shrimp Lethality Test (BSLT) method. The purpose of this research is to identify the secondary metabolites, extract characterize, and determine the LC50 value of the extract against larvae shrimp Artemia salina Leach. Screening result showed that n-hexane extract of kersen leaves contain triterpenoid. Extract characterization showed the results water content of 2.59±0.18 %w/w, water soluble extractive content of 1.9%±0.19 %w/w, the ethanol soluble extractive content of 17.41±1,87 %w/w and total ash value 0.25% w/w. Extract showed LC50 value is 278,72 ppm were calculated by probit analysis. The LC50 value indicated that the n-hexane extract of kersen leaves potentially has cytotoxic activity.

scholarly journals Leishmanicidal, Antifungal, and Cytotoxic Activity of Triterpenoid Glycosides Isolated from the Sea Cucumber Neothyone Gibbosa

2006 ◽  
Vol 1 (7) ◽  
pp. 1934578X0600100 ◽  
Author(s):  
Rosalba Encarnacion-Dimayuga ◽  
Jesús Iván Murillo-Álvarez ◽  
Carsten Christophersen ◽  
Manuel Chan-Bacab ◽  
María Luz García Reiriz ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Sea Cucumber ◽  
Antimicrobial Effect ◽  
Artemia Salina ◽  
The Body ◽  
Leishmania Mexicana ◽  
Triterpenoid Glycosides ◽  
C Nmr

Neothyosides A - C, isolated from the body walls of the sea cucumber Neothyone gibbosa Deichmann, and a partially purified mixture of these saponins, were tested in vitro against promastigotes of two strains of Leishmania mexicana and a panel of several fungi using the broth microdilution technique. In addition, the cytotoxicity of these saponins was tested against Artemia salina. The IR, 1H and 13C NMR spectroscopic and FAB MS data of neothyoside C revealed that its structure was identical to that of pervicoside B, isolated from another sea cucumber species, Holothuria pervicax. Neothyoside C was the most active antiparasitic compound, inhibiting 100% of the promastigotes at 5 and 10 μg/mL of both L. mexicana strains. The mixture of saponins and pure neothyoside C showed the best activity against Aspergillus niger strains with MIC values between 15.6 μg/mL and 16.7 μg/mL for the mixture, and 4.65 μg/mL and 7.8 μg/mL for neothyoside C. Assays for cytotoxicity using Artemia salina showed that neothyosides A and C caused 50% brine-shrimp deaths at 130 and 63 μg/mL with this cytotoxicity 4 to 6 times greater than the concentration at which these compounds produce an antimicrobial effect. This is the first report describing the antileishmanial, antifungal, and cytotoxic activity of N. gibbosa saponins.

Biosynthesis of the Diguanosine Nucleotides. I. Purification and Properties of an Enzyme from Yolk Platelets of Brine Shrimp Embryos

1974 ◽  
Vol 52 (3) ◽  
pp. 231-240 ◽  
Author(s):  
A. H. Warner ◽  
P. C. Beers ◽  
F. L. Huang
Keyword(s):  
Molecular Weight ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Temperature Optimum ◽  
Small Molecular Weight ◽  
Ph Optimum ◽  
Optimal Activity ◽  
Purification And Properties

An enzyme that catalyzes the synthesis of P1P4-diguanosine 5′-tetraphosphate (Gp4G) has been isolated and purified from yolk platelets of encysted embryos of the brine shrimp, Artemia salina. The enzyme GTP:GTP guanylyltransferase (Gp4G synthetase) utilizes GTP as substrate, has a pH optimum of 5.9–6.0, a temperature optimum of 40–42 °C, and requires Mg2+ and dithiothreitol for optimal activity. The synthesis of Gp4G is inhibited markedly by pyrophosphate, whereas orthophosphate has no effect on the reaction. In the presence of GDP the enzyme also catalyzes the synthesis of P1,P3-diguanosine 5′-triphosphate (Gp3G), but the rate of synthesis is low compared with Gp4G synthesis and dependent upon other small molecular weight components of yolk platelets.

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