scholarly journals Skrining Fitokimia dan Uji Aktivitas Sitotoksik dari Tumbuhan Sarang Semut (Myrmecodia beccarii Hook.f.) Asal Kabupaten Merauke

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Modustriati P. Mardany ◽  
Linus Y. Chrystomo ◽  
Aditya K. Karim
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Medicinal Plant ◽  
Cytotoxic Activity ◽  
Active Compound ◽  
Brine Shrimp ◽  
Ethanolic Extract ◽  
Artemia Salina ◽  
Test Method ◽  
High Cytotoxic Activity

Sarang semut (Myrmecodia beccarii Hook.f.) is a famous medicinal plant of Papua, which has traditionally potential in treating a wide variety of mild and severe illnesses, such as cancers and tumors, gout, coronary heart disease, hemorrhoids, tuberculosis, migraines, rheumatism, and leukemia. The purposes of this study were to determine the content of the active compound group and the cytotoxic activity of sarang semut plants from Merauke Region. The extraction was done by maceration using 96 % ethanol. The concentration of ethanolic extract were 0, 25, 50, 75, 100 ppm. The results of the study showed that the cytotoxic activity determine using BSLT (Brine Shrimp Lethality Test) method have a high cytotoxic activity with LC50 values of 22.86 ppm and containing the active compound namely flavonoids, tannins and saponins separately. The ethanolic extract of tuber of sarang semut (M. beccarii) has a high cytotoxic activity against larvae of Artemia salina Leach according BSLT method. Key words: Active compound, M. beccarii, cytotoxic activity, Merauke.

scholarly journals Antibacterial and Cytotoxic Activity of Longiverbenone Isolated from the Rhizome of Cyperus scariosu

1970 ◽  
Vol 25 (1) ◽  
pp. 82-84 ◽  
Author(s):  
M Shafiqur Rahman ◽  
M Nural Anwar
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Artemia Salina ◽  
Diffusion Method ◽  
Chromatographic Technique

Longiverbenone is a naturally occurring sesquiterpene isolated from ethanolic extract of Cyperus scariosus rhizome by solvent-solvent portioning and chromatographic technique. The antibacterial activity of longiverbenone was evaluated against eleven potential human pathogenic bacteria using disc diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth macrodilution method. Cytotoxic activity (lethal concentration 50%, LC50) of longiverbenone was determined on new borne brine shrimp (Artemia salina). Longiverbenone showed moderate to good antibacterial activity against the test organisms tested herein. It exhibited the lowest MIC (20 μg/ml) and MBC (80 μg/ml) against Vibrio cholerae. The LC50 of the isolated sesquiterpene was found to be 14.38 μg/ml against new borne brine shrimp. Keywords: Antibacterial activity; Cytotoxic activity (LC50); Cyperus scariosu; Longiverbenone; Minimum inhibitory concentration (MIC)DOI: http://dx.doi.org/10.3329/bjm.v25i1.4866 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 82-84

scholarly journals UJI AKTIVITAS SITOTOKSIK EKSTRAK METANOL BUAH KETUMBAR (Coriandrum sativum Linn) TERHADAP ARTEMIA SALINA LEACH DENGAN UJI BSLT (Brine Shrimp Lethality Test)

2020 ◽  
Vol 2 (3) ◽  
pp. 137-146
Author(s):  
Mega Yulia ◽  
Rani Anggraini ◽  
Farizal Farizal
Keyword(s):  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Coriandrum Sativum ◽  
Brine Shrimp Lethality ◽  
Activity Test ◽  
Anti Cancer ◽  
Expected Benefits

Coriander is a common herb can be used as traditional medicine. One of the expected benefits of Coriander fruits is an anti-cancer. This study aims to determine the cytotoxic activity of Coriander extract (Coriandrum sativum Linn) on shrimp larvae (Artemia salina Leach) with the BSLT (Brine Shrimp Lethality Test) method. Coriander fruits was extracted using methanol solvent and maceration method, then concentrated it with a rotary evaporator until a thick extract. Cytotoxic activity test used methanol extract with several concentration : 1,000 ppm, 100 ppm, 10 ppm, and 1 ppm. The result of this research reveal that methanol extract of Coriander fruits has cytotoxic activity with LC50 value is 32.35 ppm.

scholarly journals UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2019 ◽  
Vol 7 (3) ◽  
pp. 265
Author(s):  
Faradila Y. Karim ◽  
Nickson J Kawung ◽  
Billy Th. Wagey
Keyword(s):  
Cytotoxic Activity ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Bioactive Compound ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Thalassia Hemprichii ◽  
Brine Shrimp Lethality ◽  
A Value ◽  
Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)  

Assessment of Cytotoxic Activity of Agave cantula Using Brine Shrimp (Artemia salina) Lethality Bioassay

2010 ◽  
Vol 4 (1) ◽  
pp. 90-94 ◽  
Author(s):  
S. Ramachandr ◽  
M. Vamsikrish ◽  
K.V. Gowthami ◽  
B. Heera ◽  
M.D. Dhanaraju
Keyword(s):  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Artemia Salina

Cytotoxic Activities of Endophytic Fungi Isolated from the Endangered, Chinese Endemic Species Dysosma pleiantha

2009 ◽  
Vol 64 (7-8) ◽  
pp. 518-520 ◽  
Author(s):  
Yin Lu ◽  
Shaoyuan Chen ◽  
Ben Wang
Keyword(s):  
Medicinal Plant ◽  
Cytotoxic Activity ◽  
Endophytic Fungi ◽  
Endemic Species ◽  
Brine Shrimp ◽  
Cytotoxic Activities ◽  
Antitumour Agents ◽  
Endemic Medicinal Plant ◽  
Trichoderma Citrinoviride ◽  
Promising Source

Eleven strains of endophytic fungi which habitat in an endangered, Chinese endemic medicinal plant, Dysosma pleiantha (Hance) Woodson, were isolated and tested for their cytotoxic activity using the brine shrimp lethality bioassay. Six isolates were found to exhibit some cytotoxic activity. Extracts of F1273, F1276, and F1280, which were identified as Trichoderma citrinoviride, Chaetomium globosum and Ascomycete sp., in particular, showed most potent activity with LC50 values of 4.86, 7.71, and 14.88 μg/ml, respectively. These results indicate that endophytic fungi of Dysosma pleiantha could be a promising source for antitumour agents.

scholarly journals Uji Toksisitas Akut Ekstrak dan Fraksi Kulit Batang Ketapang Laut (Terminalia Catappa L.) Menggunakan Metode BSLT

2020 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Ari Sartinah ◽  
Yamin Yamin ◽  
Muhammad Arba ◽  
Nur Illiyyin Akib ◽  
Andi Nafisah Tendri Adjeng ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Program Analysis ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Brine Shrimp Lethality ◽  
Terminalia Catappa ◽  
Water Fractions

AbstrakToksisitas akut merupakan kemampuan suatu bahan kimia (obat/bahan obat) dalam menimbulkan kerusakan  pada suatu organisme dalam waktu yang relatif singkat. Tujuan penelitian ini adalah untuk mengetahui potensi ketoksikan akut dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L.). Pengujian toksisitas akut dilakukan dengan menggunakan metode BSLT (Brine Shrimp Lethality Test) dan menggunakan analisis program untuk mengetahui nilai LC50 dari ekstrak dan fraksi. Hasil uji toksisitas akut menunjukan nilai LC50  dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L. )  berturut-turut 247,997 ppm; 400,666 ppm dan 618,046 ppm. Hal ini menunjukan bahwa ekstrak metanol batang ketapang laut (Terminalia catappa L.) bersifat paling toksik dibandingkan fraksi etil asetat dan fraksi air.Kata kunci: Ekstrak, Fraksi, Terminalia catappa L., BSLT, Artemia salina LeachAbstractAcute toxicity is the ability of chemical (drug/drug ingredient) to cause damage to organisms in a relatively short time. The purpose of this study was to determine the potential for acute toxicity of methanol extract, ethyl acetate and water fractions of sea ketapang stem skin (Terminalia catappa L.). Acute toxicity testing was performed using the BSLT (Brine Shrimp Lethality Test) method and program analysis to determine the LC50 values of extracts and fractions. The results of the acute toxicity test showed the LC50 values of methanol extract, ethyl acetate and water fractions of the plant stem skin consecutively were 247,997 ppm; 400,666 ppm and 618,046 ppm. This shows that the methanol extract of sea ketapang stems (Terminalia catappa L.) is the most toxic compared to the ethyl acetate and the water fraction.Keywords: Extract, Fraction, Terminalia catappa L., BSLT, Artemia salina Leach

Toksisitas Akut Ektrak N-Heksan Dan Methanol Rimpang Jeringau Merah Terhadap Kematian Artemia Salina Leach Dengan Metode Brine Shrimp Lethality Test

2018 ◽  
Vol 1 (2) ◽  
pp. 125
Author(s):  
Edy Suwandi ◽  
Supriyanto Supriyanto
Keyword(s):  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Test Method ◽  
Endemic Plant ◽  
Brine Shrimp Lethality ◽  
Purposive Sampling ◽  
Study Results ◽  
Sweet Flag

Abstract: Acorus sp (red sweet flag) is an endemic plant from West Borneo which contains chemical substances such as flavonoids that have bioactivity as antioxidants so it is recommended one to prevent cancer. The study aimed to determine toxicity of n-Hexan extract and methanol red sweet flag rhizomes against Artemia salina Leach with Brine Shrimp Lethality Test method. This study was a type of quasi experimental design, sampling technique obtained by using purposive sampling. According to the study results, it can be seen than methanol extract from red sweet flag rhizomes give impact on the mortality average of Artemia salina Leach : concentration 100 µg/ml (51%), 250 µg/ml (75%), 500 µg/ml (92%), 750 µg/ml (96%) and 1000 µg/ml (99%). In addition, n-Hexan extract of red sweet flag rhizomes in 100 µg/ml (45%), 250 µg/ml (60%), 500 µg/ml (92%), 750 µg/ml (84%) and 1000 µg/ml (96%). It is reported based on probit analysis that LC50 of n-Hexan extract from red sweet flag rhizomes was 128.2921 µg/ml meanwhile methanol extract was 242.7169 µg/ml µg/ ml, thus in conclusion n-Hexan and methanol extract of red sweet flag rhizomes potentially cytotoxic to Artemia salina Leach larvae.Abstrak: Jeringau merah (Acorus sp) merupakan satu jenis tanaman endemik Kalimantan Barat memiliki kandungan kimia diantaranya flavonoidyang mempunyai bioaktivitas sebagai antioksidan sehingga sangat baik untuk pencegahan kanker. Penelitian ini bertujuan mengetahui mengetahui dan menentukan toksisitas ekstrak n-Heksan dan Methanol Rimpang Jeringau MerahterhadapArtemia salinaLeachdengan metode Brine Shrimp Lethality Test. Desain penelitian ini adalah eksperimental semu, teknik pengambilan sampel dengan cara purposive sampling. Berdasarkan hasil penelitian diketahui ekstrak Methanol Rimpang Jeringau Merah rerata kematianArtemia salina Leach konsentrasi 100 µg/ml (51%), 250 µg/ ml (75%), 500 µg/ml (92%), 750 µg/ml (96%) dan 1000 µg/ml (99%)pada ekstrak n-Heksan Rimpang Jeringau Merah konsentrasi 100 µg/ml (45%), 250 µg/ml (60%), 500 µg/ml (92%), 750 µg/ml (84%) dan 1000 µg/ml (96%). Dari analisis probit diketahuinilai LC50 ekstrak n-Heksan Rimpang Jeringau Merah yaitu 128.2921 µg/ml dan ekstrak Methanol Rimpang Jeringau Merah yaitu 242.7169 µg/ml µg/ml, maka disimpulkan ekstrak n-Heksan dan Methanol Rimpang Jeringau Merahbersifat sitotoksik terhadap larva Artemia salina Leach.

Assessment of biological activity of brazilian medicinal plants against Artemia Salina Leach

2010 ◽  
Vol 4 (4) ◽  
pp. 1639
Author(s):  
Rinalda Araújo Guerra de Oliveira ◽  
Marcus Antônio de Brito Folly ◽  
Aline Lira Xavier ◽  
Margareth De Fátima Formiga Melo Diniz ◽  
Edeltrudes De Oliveira Lima
Keyword(s):  
Biological Activity ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Momordica Charantia ◽  
Test Method ◽  
Artificial Light ◽  
Lc50 Value ◽  
Pharmacological Studies ◽  
Ipomoea Asarifolia ◽  
Brazilian Medicinal Plants

ABSTRACT Objective: to evaluate the biological activity of extracts of Pithecellobium cochliocarpum, Momordica charantia and Ipomoea asarifolia isolated and associated with the Brine Shrimp test. Method: solutions of different concentrations of the extracts was prepared and metanauplius of Artemia salina were added. Each concentration was tested in triplicate, repeated in two experiments. The set was left in incubation under artificial light for 24 hours and after this time the counting of the number of live larvae and dead was done. It was determined the LC50 in accordance with the statistical method of Probits. Results: the LC50 value obtained for the extracts of Pithecellobium cochliocarpum, Momordica charantia and Ipomoea asarifolia when tested alone was: 543.5 ppm, 400.9 ppm and 916.3 ppm, respectively. For the association of Pithecellobium cochliocarpum and Ipomoea asarifolia, Pithecellobium cochliocarpum and Momordica charantia, and Momordica charantia and Ipomoea asarifolia, the LC50 found was respectively: 641.0 ppm, 502.2 ppm and 706.4 ppm. The association of the three extracts showed an LC50 of 990.8 ppm. Conclusion: all extracts showed biological activity against Artemia salina and their associations can lead to interactions among its constituents. More detailed pharmacological studies should be conducted. Descriptors: Artemia; Plants medicinal; toxicity; associations.RESUMOObjetivo: avaliar a atividade biológica dos extratos de Pithecellobium cochliocarpum, Momordica charantia e Ipomoea asarifolia isolados e associados através do bioensaio com Artemia salina. Método: prepararam-se soluções de diferentes concentrações dos extratos e a elas foram adicionados metanáuplios de Artemia salina. Cada concentração foi testada em triplicata, repetida em dois experimentos. Deixou-se o conjunto em incubação sob luz artificial por 24 h e após esse período realizou-se a contagem do número de larvas vivas e mortas. Determinou-se a CL50 de acordo com o método estatístico de Probitos. Resultados: o valor da CL50 obtido para os extratos de Pithecellobium cochliocarpum, Momordica charantia e Ipomoea asarifolia quando testados isoladamente foi de: 543,5 ppm, 400,9 ppm e 916,3 ppm, respectivamente. Para a associação de Pithecellobium cochliocarpum e Momordica charantia, Pithecellobium cochliocarpum e Ipomoea asarifolia, e Momordica charantia e Ipomoea asarifolia, a CL50 encontrada foi, respectivamente: 641,0 ppm, 502,2 ppm e 706,4 pppm. Já a associação dos três extratos apresentou uma CL50 de 990,8 ppm. Conclusão: Todos os extratos apresentaram atividade biológica frente Artemia salina. E suas associações podem levar à interações entre seus constituintes. Estudos farmacológicos mais aprofundados devem ser realizados. Descritores: Artemia; plantas medicinais; toxicidade; associações.RESUMENObjetivo: evaluar la actividad biológica de extractos de Pithecellobium cochliocarpum, Momordica charantia y Ipomoea asarifolia aislada y asociada con el bioensayo con Artemia salina. Método: de las soluciones preparadas de diferentes concentraciones de los extractos y se agregaron metanauplius de Artemia salina. Cada concentración se analizó por triplicado, repetida en dos experimentos. Salió de la serie en la incubación bajo luz artificial durante 24 horas después de ese momento en poder contar el número de larvas vivas y muertas. Se determinó la CL50 de conformidad con el método estadístico de Probits. Resultados: el valor de la CL50 obtenidos para los extractos de Pithecellobium cochliocarpum, Momordica charantia y Ipomoea asarifolia durante la prueba era el único: 543,5 ppm, 400,9 ppm y 916,3 ppm, respectivamente. Para la asociación de Pithecellobium cochliocarpum y Momordica charantia, Pithecellobium cochliocarpum y Ipomoea asarifolia, y Momordica charantia y Ipomoea asarifolia, la CL50 fue encontrado, respectivamente: 641,0 ppm, 502,2 ppm y 706,4 PPPM. La asociación de los tres extractos mostraron un CL50 de 990,8 ppm. Conclusión: Todos los extractos mostraron actividad biológica contra Artemia salina. Y sus asociaciones pueden dar lugar a interacciones entre sus componentes. Estudios más detallados farmacológico debe llevarse a cabo. Descriptores: Artemia; plantas medicinales; toxicidad; associaciones. 

scholarly journals Brine Shrimp (Artemia salina Leach.) Lethality Test of Ethanolic Extract from Green Betel (Piper betle Linn.) and Red Betel (Piper crocatum Ruiz and Pav.) through the Soxhletation Method for Cytotoxicity Test

2021 ◽  
Vol 9 (A) ◽  
pp. 407-412
Author(s):  
Nerdy Nerdy ◽  
Puji Lestari ◽  
Jon Piter Sinaga ◽  
Selamat Ginting ◽  
Nilsya Febrika Zebua ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Ethanolic Extract ◽  
Artemia Salina ◽  
Lethal Concentration ◽  
Piper Betle ◽  
Cytotoxicity Test ◽  
Pharmacological Effects ◽  
Phytochemical Content ◽  
Anticancer Properties ◽  
Lc50 Value

Traditional medicine is currently being explored and developed widely by various countries because its cost effectiveness and reduced side effects. The green betel (Piper betle Linn.) and the red betel (Piper crocatum Ruiz and Pav.) are plants from the same Piper genus that are widely cultivated in Indonesia and have various phytochemical properties and pharmacological effects. The objective of this research is to determine the cytotoxicity of green leaves and red betel leaves. This research was conducted in two main stages: first, the extraction and preparation through the soxhletation method; second, a cytotoxicity test through the brine shrimp (Artemia salina Leach) lethality test (BSLT). The results showed that there were lethal concentration 50 % (LC50) value in both green betel leaves ethanolic extract and red betel leaves ethanolic extract, with 44.975 μg per mL and 31.556 μg per mL, respectively. The red betel (leaves ethanolic extract has a higher cytotoxicity than green betel (leaves ethanolic extract which can be seen from the lower lethal concentration of 50% (LC50) value. The difference in cytotoxicity is due to differences in plant varieties that cause variations in phytochemical content which affects the pharmacological effects. The green betel leaves ethanolic extract and the red betel leaves ethanolic extract are cytotoxic and have potential anticancer properties.

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