Protocol optimization for rapid and efficient callus induction and in-vitro regeneration in rice (Oryza sativa L.) cv. CO 51

2020 ◽  
Keyword(s):  
Oryza Sativa ◽  
Callus Induction ◽  
Oryza Sativa L ◽  
In Vitro Regeneration ◽  
Protocol Optimization

scholarly journals Effects of Cultivation Media on In vitro Callus Induction and Regeneration Capabilities of Pakaumpuel Rice (Oryza sativa L.), Thai Rice Landrace

2018 ◽  
Vol 17 (1) ◽  
pp. 37-46
Author(s):  
Attachai TRUNJARUEN ◽  
Sayam RASO ◽  
Pitakpong MANEERATTANARUNGROJ ◽  
Worasitikulya TARATIMA
Keyword(s):  
Oryza Sativa ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Oryza Sativa L ◽  
Light Condition ◽  
Ms Medium ◽  
Rice Landraces ◽  
Rice Landrace ◽  
Number Of Leaves

Current study determined the effects of different cultivation media and some factors on in vitro callus induction and shoot regeneration of Pakaumpuel rice (Oryza sativa L.), the Thai rice landrace. Pakaumpuel’s dehusked seeds were surface sterilized by 20% Sodium hypoclorite for 20 mins before washed and cultured on Murashige and Skoog (MS) medium in various concentrations of BAP (0, 0.1, 0.5 and 1 mg/l) and 2,4-D (0, 1, 2 and 3 mg/l) and cultivated  in off -light or dark cycle condition for 15 days. Results showed that MS medium with 2 mg/l 2,4-D incubated in light condition provided the highest callus induction percentage (80 %). For shoot regeneration, calli were desiccated by using silica gel for 0, 30, 90 and 180 minutes prior to culture on MS medium with 1 mg/l NAA and various concentrations of BAP (0, 1, 2, 3 and 4 mg/l) for 30 days. The results revealed that desiccation periods had no effect on regeneration percentage and number of shoot per callus, but had effects on shoot length and number of leaves per shoot. Therefore, MS medium with 1 mg/l NAA and 3 mg/l BAP induced the highest regeneration percentage (53.33 %) and number of shoot per callus (4.40 shoots), while the longest shoot (12.82 cm) and the highest number of leaves per shoot (3.00 leaves) were found on the culture on this medium after desiccation for 90 minutes. This discovery can be applied for micropropagation of other Thai rice landraces.

scholarly journals Optimization of in vitro regeneration protocol for a popular Indica rice (Oryza sativa L. cv Swarna)

2016 ◽  
Vol 5 (08) ◽  
pp. 1395 ◽  
Author(s):  
Vijaya Naresh Juturu ◽  
Gopala Krishna Mekala ◽  
Mallikarjuna Garladinne ◽  
Puli Chandra Obul Reddy ◽  
Akila Chandra Sekhar*
Keyword(s):  
Callus Induction ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Rice Varieties ◽  
Regenerated Plants ◽  
Rooting Medium

Though regeneration system in rice has been very well established compare to other crop plants, the fact remains that, most of the indica rice varieties are still recalcitrant for regeneration and genetic transformation. Therefore, refinement of tissue culture protocol for generation of embryogenic calli and regeneration of the fertile plants from a single cell should be a pre requisite event for development of transgenic plants. Here, in this study we reported high frequency robust regeneration protocols for a popular Indica cultivar Swarna.Mature seeds were used as initial material as explants. Highest callus induction % was observed in MSCIMP medium containing 2.0 mg-1 2,4, D + 0.5 mg-1 Kn as phytohormonal combinations. In addition, maximum regeneration was observed in 2.0 mg-l KN + 0.5 mg-l NAA. Regenerated plants were shifted to rooting medium followed by polyhouse for hardening. The callus induction and regeneration reported in this study were well suited for transformation agronomical important genes or functional genomics studies.

Callus Induction and in vitro Plant Regeneration of Rice (Oryza sativa L.) Under Various Conditions

2008 ◽  
Vol 11 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Muhammad Tariq ◽  
Gowher Ali ◽  
Fazal Hadi ◽  
Shakeel Ahmad ◽  
Nasir Ali ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Oryza Sativa L ◽  
In Vitro Plant Regeneration ◽  
In Vitro Plant

scholarly journals In Vitro Regeneration of High Yielding Indica Rice (Oryza sativa L.) Varieties

2012 ◽  
Vol 5 (1) ◽  
pp. 173-177 ◽  
Author(s):  
MJ Alam ◽  
M Imran ◽  
L Hassan ◽  
MH Rubel ◽  
M Shamsuddoha
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Culture Media ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration ◽  
Induction Frequency ◽  
Establishment Rate ◽  
Number Of Shoots

The experiment was conducted to observe the regeneration potential and also to establish a suitable in vitro plantlet regeneration protocol from mature seed derived embryogenic calli of four indica rice varieties viz BRRI dhan28, BRRI dhan29, BRRI dhan47 and Binadhan-7 after partial desiccation treatment. Different concentrations and combinations of growth regulators were used in MS medium to observe the callus induction ability using mature embryo as explants. The percentage of callus induction frequency  was highest (86.00) in BRRI dhan47 and the lowest (56.50) in Binadhan-7 Among the culture media the performance of MS +500 mgl-1 L- Proline + 2.0 mgl-12, 4-D + 0.8 mgl-1BAP was better than any other media for callus induction frequency (%), rapid  callusing, size of the callus (mm), texture of callus and color of callus. Among the four varieties, shoot regeneration was highest in BRRI dhan29 (84.33%) which required minimum (14.80) days to and the lowest was in Binadhan-7 (39.67%) which required maximum (15.47) days. Among the treatments, the highest (65.75%) shoot regeneration was observed with MS + 6.0 mgl-1Kn +0.5 mgl-1 NAA which required minimum days (13.75) to develop green bud formation and the lowest shoot regeneration (56.50) was observed with MS + 2.0 mgl-1Kn + .05 mgl-1 NAA. BRRI dhan29 produced more number of shoots (4.67) per callus while Binadhan-7 showed minimum number of shoots (2.87) per callus. The highest number of shoot producing roots (3.66) was observed in BRRI dhan29 which showed maximum number of root per plant and the lowest (3.11) in Binadhan-7. Among the three treatments MS + 0.6 mgl-1IBA showed highest percentage 86.67 of root followed by MS+ 0.6 mgl-1IBA 70% and the lowest (66.7%) was in MS+ 0.4 mgl-1IBA. The establishment rate of the plantlet in the pot was the highest (67.67%) in BRRI dhan29 and Binadhan-7 showed lowest establishment rate (51.22%) in pot. DOI: http://dx.doi.org/10.3329/jesnr.v5i1.11573J. Environ. Sci. & Natural Resources, 5(1): 173 - 177, 2012

Investigating the In vitro Regeneration Potentiality of Three High Yielding Indica Rice (Oryza sativa L.) Varieties

2015 ◽  
Vol 9 (3) ◽  
pp. 1-13
Author(s):  
Mohammd Hossain ◽  
Nazneen Akter ◽  
Upama Mondal ◽  
Ratan Dey ◽  
Lutful Hassan
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration

scholarly journals In Vitro Callus Induction and Regeneration Potentiality of Aromatic Rice (Oryza sativa L.) Cultivars in Differential Growth Regulators

2014 ◽  
Vol 2 (2) ◽  
pp. 160-167 ◽  
Author(s):  
Zahida Yesmin Roly ◽  
Md. Mahmudul Islam ◽  
Md. Pallob Ebna Shaekh ◽  
Md. Saiful Islam Arman ◽  
Shah Md. Shahik ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Callus Induction ◽  
Oryza Sativa L ◽  
Immature Embryo ◽  
Aromatic Rice ◽  
Rice Cultivars ◽  
Differential Growth ◽  
Soil Constraints ◽  
Important Trait

Abstract Aromatic rice (Oryza sativa L.) cultivars are strong aromatic rice cultivars which can thrive well in rice fields prone to flood, drought and other soil constraints. The present investigation was undertaken to determine a suitable media compositions for callus induction and regeneration using immature embryo of six aromatic grown rice cultivars of Bangladesh, namely, Chinigura, Kalijira, Radhuni Pagal, Modhumala, Kataribog and Mohonbhog. For callus induction different concentrations and combinations of 2, 4-Dichlorophenoxyaceticacid (2, 4-D) along with NAA were evaluated. Maximum callus induction (97.22%) was observed in Kalijira when 2 mg/L of 2, 4-D and 0.5 mg/l NAA was used and less Modhumala (66.67%) and remaining cultivars showed moderate. For regeneration initially different concentrations and combinations of 6-BenzylAminoPurine (BAP) and Indole-3-Acetic Acid (IBA) were tested. Maximum regeneration frequency (91.67%) was observed Kalijira when the optimum concentrations and combinations of 0.5 mg/l of BAP + 0.1 of mg/l IBA were used. Presently optimized regeneration method holds promise for facilitating the deployment of agronomical important trait through genetic transformation for the improvement of this important food crops.DOI: http://dx.doi.org/10.3126/ijasbt.v2i2.10313Int J Appl Sci Biotechnol, Vol. 2(2): 160-167 

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