Investigating the In vitro Regeneration Potentiality of Three High Yielding Indica Rice (Oryza sativa L.) Varieties

2015 ◽  
Vol 9 (3) ◽  
pp. 1-13
Author(s):  
Mohammd Hossain ◽  
Nazneen Akter ◽  
Upama Mondal ◽  
Ratan Dey ◽  
Lutful Hassan
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration

Improvement of Indica rice (Oryza sativa L.) in vitro regeneration efficiency from callus mediated by stress

1998 ◽  
Vol 26 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Yamilet Coll ◽  
Merardo Pujol ◽  
Damaris Castillo ◽  
Annerys González ◽  
Julio Alfonso ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration ◽  
Regeneration Efficiency

In vitro flowering of indica rice (Oryza sativa L. spp. indica)

2012 ◽  
Vol 48 (2) ◽  
pp. 259-264 ◽  
Author(s):  
Suriyan Cha-um ◽  
Thapanee Samphumphuang ◽  
Chalermpol Kirdmanee
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Flowering

scholarly journals Optimization of in vitro regeneration protocol for a popular Indica rice (Oryza sativa L. cv Swarna)

2016 ◽  
Vol 5 (08) ◽  
pp. 1395 ◽  
Author(s):  
Vijaya Naresh Juturu ◽  
Gopala Krishna Mekala ◽  
Mallikarjuna Garladinne ◽  
Puli Chandra Obul Reddy ◽  
Akila Chandra Sekhar*
Keyword(s):  
Callus Induction ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Rice Varieties ◽  
Regenerated Plants ◽  
Rooting Medium

Though regeneration system in rice has been very well established compare to other crop plants, the fact remains that, most of the indica rice varieties are still recalcitrant for regeneration and genetic transformation. Therefore, refinement of tissue culture protocol for generation of embryogenic calli and regeneration of the fertile plants from a single cell should be a pre requisite event for development of transgenic plants. Here, in this study we reported high frequency robust regeneration protocols for a popular Indica cultivar Swarna.Mature seeds were used as initial material as explants. Highest callus induction % was observed in MSCIMP medium containing 2.0 mg-1 2,4, D + 0.5 mg-1 Kn as phytohormonal combinations. In addition, maximum regeneration was observed in 2.0 mg-l KN + 0.5 mg-l NAA. Regenerated plants were shifted to rooting medium followed by polyhouse for hardening. The callus induction and regeneration reported in this study were well suited for transformation agronomical important genes or functional genomics studies.

Sign in / Sign up

Export Citation Format

Share Document