scholarly journals Effects of different plant growth hormones on in vitro regeneration of a new Malaysian rice variety (Oryza Sativa L. Var. Mria 1) from stem explants

2016 ◽  
Vol 2 (4) ◽  
Keyword(s):  
Oryza Sativa ◽  
Plant Growth ◽  
Oryza Sativa L ◽  
In Vitro Regeneration ◽  
Rice Variety ◽  
Growth Hormones ◽  
Stem Explants ◽  
Plant Growth Hormones

scholarly journals Detection of Somaclonal Variation in Micropropagated and Acclimatized Plantlets of Oryza sativa MRQ 74 from Stem Explants

2019 ◽  
Vol 37 ◽  
Author(s):  
A. SALEH ◽  
R.M. TAHA ◽  
N. MAHMAD ◽  
H. ELIAS ◽  
H.A.A. HAMID
Keyword(s):  
Oryza Sativa ◽  
Plant Growth ◽  
Somaclonal Variation ◽  
Oryza Sativa L ◽  
Growth Hormones ◽  
Optimum Concentration ◽  
Cytological Analysis ◽  
Plant Growth Hormones

ABSTRACT: In plant tissue culture system, the excessive supply of plant growth hormones may inhibit further growth and development of the regenerants and sometimes it may induce somaclonal variation. In this study, the optimum concentration of plant growth hormones for in vitro regeneration of Oryza sativa L. cv. MRQ 74 was identified and subsequently the occurrence of somaclonal variation of regenerated plantlets was determined through cytological analysis of in vivo and in vitro grown plantlets. MS media supplemented with 0.1 mg L-1 BAP in combination with 0.1 mg L-1 NAA was found to be the optimum concentration for shoots and roots induction of Oryza sativa L. cv. MRQ 74. Plantlets derived from MS media supplemented with 0.5 mg L-1 2,4-D showed higher survival rate than that of MS media supplemented with 0.1 mg L-1 BAP in combination with 0.1 mg L-1 NAA. The plant height, number of leaf, leaf length and number of seeds per stalk of acclimatized plants were significantly lower (p<0.05) as compared with plants grown in vivo. Cytological analysis of in vivo and in vitro grown Oryza sativa L. cv. MRQ 74 revealed that the presence of 2,4-D in the culture media had significantly decreased the mitotic index (MI) and increased the ploidy level of the cell nuclei. The findings of the present study would be useful for plant breeders and biotechnologist since somaclonal variation provides a useful source of genetic variation for crop productivity and quality improvement.

scholarly journals Optimization of selected plant growth regulators on callus induction of Oryza sativa L. var MR 219

Food Research ◽  
2021 ◽  
Vol 5 (S4) ◽  
pp. 38-45
Author(s):  
Siti Nur Adila H. ◽  
Nor Azma Y.
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Rice Variety ◽  
Crop Improvement ◽  
Naphthalene Acetic Acid

MR 219, an indica rice from a cross between MR 137 and MR 151 is a long-grain rice variety of high-yielding, good quality of shape and taste, short maturation as well as resistance to blast and bacterial leaf blight. However, it is regarded as a recalcitrant variety due to its low ratio of callogenesis and regeneration in in vitro culturing. This would be a hindrance to crop improvements such as genetic transformation and other crop improvement methods. The objective of this study was to optimize the concentrations and combinations of plant growth regulators (PGRs) which were 2,4-Dichlorophenoxyacetic acid (2,4-D), Kinetin (KIN) and Naphthalene acetic acid (NAA) on its callus induction that ranging from 0.5 to 15.0 mg/L. The callus was induced from the mature seeds of MR 219 on the Murashige and Skoog (MS) media supplemented with 4.4 g/L of MS powder with vitamins, 30.0 g/L of sucrose and 3.5 g/L of gelrite with the addition of 2,4-D, KIN and NAA in different concentrations and combinations. All media supplemented with 2,4- D had successfully induced the callus and 2.0 mg/L of 2,4-D was the best concentration for the callus induction with a 100% success rate. The addition of 15.0 mg/L of NAA, provided less time taken for callus induction with better callus morphology which resulted in fewer browning problems.

Investigating the In vitro Regeneration Potentiality of Three High Yielding Indica Rice (Oryza sativa L.) Varieties

2015 ◽  
Vol 9 (3) ◽  
pp. 1-13
Author(s):  
Mohammd Hossain ◽  
Nazneen Akter ◽  
Upama Mondal ◽  
Ratan Dey ◽  
Lutful Hassan
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration

Improvement of Indica rice (Oryza sativa L.) in vitro regeneration efficiency from callus mediated by stress

1998 ◽  
Vol 26 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Yamilet Coll ◽  
Merardo Pujol ◽  
Damaris Castillo ◽  
Annerys González ◽  
Julio Alfonso ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration ◽  
Regeneration Efficiency
Sign in / Sign up

Export Citation Format

Share Document