Calcium Imaging in Freely Behaving Caenorhabditis elegans with Well-Controlled, Nonlocalized Vibration

10.3791/61626 ◽  
2021 ◽  
Author(s):  
Kazuki Shigyou ◽  
Haruka Maeoka ◽  
Ryuji Igarashi ◽  
Takuma Sugi
Keyword(s):  
Caenorhabditis Elegans ◽  
Calcium Imaging ◽  
Freely Behaving

scholarly journals Whole-brain calcium imaging with cellular resolution in freely behaving Caenorhabditis elegans

2015 ◽  
Vol 113 (8) ◽  
pp. E1074-E1081 ◽  
Author(s):  
Jeffrey P. Nguyen ◽  
Frederick B. Shipley ◽  
Ashley N. Linder ◽  
George S. Plummer ◽  
Mochi Liu ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Animal Behavior ◽  
Calcium Imaging ◽  
Large Scale ◽  
Calcium Transients ◽  
Whole Brain ◽  
Cellular Resolution ◽  
Calcium Indicator ◽  
Custom Software ◽  
Freely Behaving

The ability to acquire large-scale recordings of neuronal activity in awake and unrestrained animals is needed to provide new insights into how populations of neurons generate animal behavior. We present an instrument capable of recording intracellular calcium transients from the majority of neurons in the head of a freely behaving Caenorhabditis elegans with cellular resolution while simultaneously recording the animal’s position, posture, and locomotion. This instrument provides whole-brain imaging with cellular resolution in an unrestrained and behaving animal. We use spinning-disk confocal microscopy to capture 3D volumetric fluorescent images of neurons expressing the calcium indicator GCaMP6s at 6 head-volumes/s. A suite of three cameras monitor neuronal fluorescence and the animal’s position and orientation. Custom software tracks the 3D position of the animal’s head in real time and two feedback loops adjust a motorized stage and objective to keep the animal’s head within the field of view as the animal roams freely. We observe calcium transients from up to 77 neurons for over 4 min and correlate this activity with the animal’s behavior. We characterize noise in the system due to animal motion and show that, across worms, multiple neurons show significant correlations with modes of behavior corresponding to forward, backward, and turning locomotion.

Fast whole‐body motor neuron calcium imaging of freely moving Caenorhabditis elegans without coverslip pressed

2021 ◽  
Author(s):  
Haiwen Li ◽  
Fan Feng ◽  
Muyue Zhai ◽  
Jia Zhi Zhang ◽  
Jingyuan Jiang ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Motor Neuron ◽  
Calcium Imaging ◽  
Whole Body ◽  
Freely Moving

scholarly journals Calcium imaging of multiple neurons in freely behaving C. elegans

2012 ◽  
Vol 206 (1) ◽  
pp. 78-82 ◽  
Author(s):  
Maohua Zheng ◽  
Pengxiu Cao ◽  
Jiong Yang ◽  
X.Z. Shawn Xu ◽  
Zhaoyang Feng
Keyword(s):  
Calcium Imaging ◽  
C Elegans ◽  
Freely Behaving

scholarly journals A Compact Head-Mounted Endoscope for In Vivo Calcium Imaging in Freely Behaving Mice

2018 ◽  
Vol 84 (1) ◽  
pp. e51 ◽  
Author(s):  
Alexander D. Jacob ◽  
Adam I. Ramsaran ◽  
Andrew J. Mocle ◽  
Lina M. Tran ◽  
Chen Yan ◽  
...  
Keyword(s):  
Calcium Imaging ◽  
Freely Behaving ◽  
In Vivo Calcium Imaging

scholarly journals Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

10.3791/56911 ◽  
2018 ◽  
Author(s):  
Bhavya Ravi ◽  
Layla M. Nassar ◽  
Richard J. Kopchock ◽  
Pravat Dhakal ◽  
Michael Scheetz ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Calcium Imaging

scholarly journals Presynaptic MAST kinase controls opposing postsynaptic responses to convey stimulus valence in Caenorhabditis elegans

2020 ◽  
Vol 117 (3) ◽  
pp. 1638-1647 ◽  
Author(s):  
Shunji Nakano ◽  
Muneki Ikeda ◽  
Yuki Tsukada ◽  
Xianfeng Fei ◽  
Takamasa Suzuki ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Diacylglycerol Kinase ◽  
Inhibitory Response ◽  
Thermal Gradients ◽  
Postsynaptic Neuron ◽  
Presynaptic Neuron ◽  
Presynaptic Plasticity ◽  
Freely Behaving ◽  
Stimulus Valence ◽  
Thermal Stimuli

Presynaptic plasticity is known to modulate the strength of synaptic transmission. However, it remains unknown whether regulation in presynaptic neurons can evoke excitatory and inhibitory postsynaptic responses. We report here that the Caenorhabditis elegans homologs of MAST kinase, Stomatin, and Diacylglycerol kinase act in a thermosensory neuron to elicit in its postsynaptic neuron an excitatory or inhibitory response that correlates with the valence of thermal stimuli. By monitoring neural activity of the valence-coding interneuron in freely behaving animals, we show that the alteration between excitatory and inhibitory responses of the interneuron is mediated by controlling the balance of two opposing signals released from the presynaptic neuron. These alternative transmissions further generate opposing behavioral outputs necessary for the navigation on thermal gradients. Our findings suggest that valence-encoding interneuronal activity is determined by a presynaptic mechanism whereby MAST kinase, Stomatin, and Diacylglycerol kinase influence presynaptic outputs.

Automated imaging of neuronal activity in freely behaving Caenorhabditis elegans

2010 ◽  
Vol 187 (2) ◽  
pp. 229-234 ◽  
Author(s):  
Juliette Ben Arous ◽  
Yoshinori Tanizawa ◽  
Ithai Rabinowitch ◽  
Didier Chatenay ◽  
William R. Schafer
Keyword(s):  
Caenorhabditis Elegans ◽  
Neuronal Activity ◽  
Automated Imaging ◽  
Freely Behaving

scholarly journals Real-time multimodal optical control of neurons and muscles in freely behaving Caenorhabditis elegans

2011 ◽  
Vol 8 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Jeffrey N Stirman ◽  
Matthew M Crane ◽  
Steven J Husson ◽  
Sebastian Wabnig ◽  
Christian Schultheis ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Real Time ◽  
Optical Control ◽  
Freely Behaving
Sign in / Sign up

Export Citation Format

Share Document