scholarly journals Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

10.3791/56911 ◽  
2018 ◽  
Author(s):  
Bhavya Ravi ◽  
Layla M. Nassar ◽  
Richard J. Kopchock ◽  
Pravat Dhakal ◽  
Michael Scheetz ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Calcium Imaging

Fast whole‐body motor neuron calcium imaging of freely moving Caenorhabditis elegans without coverslip pressed

2021 ◽  
Author(s):  
Haiwen Li ◽  
Fan Feng ◽  
Muyue Zhai ◽  
Jia Zhi Zhang ◽  
Jingyuan Jiang ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Motor Neuron ◽  
Calcium Imaging ◽  
Whole Body ◽  
Freely Moving

An automated microfluidic platform for calcium imaging of chemosensory neurons in Caenorhabditis elegans

Lab on a Chip ◽  
2010 ◽  
Vol 10 (20) ◽  
pp. 2758 ◽  
Author(s):  
Trushal Vijaykumar Chokshi ◽  
Daphne Bazopoulou ◽  
Nikos Chronis
Keyword(s):  
Caenorhabditis Elegans ◽  
Calcium Imaging ◽  
Microfluidic Platform ◽  
Chemosensory Neurons

scholarly journals Whole-brain calcium imaging with cellular resolution in freely behaving Caenorhabditis elegans

2015 ◽  
Vol 113 (8) ◽  
pp. E1074-E1081 ◽  
Author(s):  
Jeffrey P. Nguyen ◽  
Frederick B. Shipley ◽  
Ashley N. Linder ◽  
George S. Plummer ◽  
Mochi Liu ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Animal Behavior ◽  
Calcium Imaging ◽  
Large Scale ◽  
Calcium Transients ◽  
Whole Brain ◽  
Cellular Resolution ◽  
Calcium Indicator ◽  
Custom Software ◽  
Freely Behaving

The ability to acquire large-scale recordings of neuronal activity in awake and unrestrained animals is needed to provide new insights into how populations of neurons generate animal behavior. We present an instrument capable of recording intracellular calcium transients from the majority of neurons in the head of a freely behaving Caenorhabditis elegans with cellular resolution while simultaneously recording the animal’s position, posture, and locomotion. This instrument provides whole-brain imaging with cellular resolution in an unrestrained and behaving animal. We use spinning-disk confocal microscopy to capture 3D volumetric fluorescent images of neurons expressing the calcium indicator GCaMP6s at 6 head-volumes/s. A suite of three cameras monitor neuronal fluorescence and the animal’s position and orientation. Custom software tracks the 3D position of the animal’s head in real time and two feedback loops adjust a motorized stage and objective to keep the animal’s head within the field of view as the animal roams freely. We observe calcium transients from up to 77 neurons for over 4 min and correlate this activity with the animal’s behavior. We characterize noise in the system due to animal motion and show that, across worms, multiple neurons show significant correlations with modes of behavior corresponding to forward, backward, and turning locomotion.

scholarly journals Escape Steering by Cholecystokinin Peptidergic Signaling

2021 ◽  
Author(s):  
Lili Chen ◽  
Yuting Liu ◽  
Pan Su ◽  
Wesley Hung ◽  
Haiwen Li ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Motor Neuron ◽  
Avoidance Response ◽  
Calcium Imaging ◽  
Genetic Screening ◽  
Cholecystokinin Receptor ◽  
Evolutionarily Conserved ◽  
Escape Responses

Escape is an evolutionarily conserved and essential avoidance response. Considered to be innate, most studies on escape responses focused on hard-wired circuits. We report here that peptidergic signaling is an integral and necessary component of the Caenorhabditis elegans escape circuit. Combining genetic screening, electrophysiology, and calcium imaging, we reveal that a neuropeptide NLP-18 and its cholecystokinin receptor CKR-1 enable the escape circuit to execute a full omega turn, the last motor step where the animal robustly steers away from its original trajectory. We demonstrate in vivo and in vitro that CKR-1 is a Gq protein-coupled receptor for NLP-18. in vivo, NLP-18 is mainly secreted by the gustatory sensory neuron (ASI) to activate CKR-1 in the head motor neuron (SMD) and the turn-initiating interneuron (AIB). Removal of NLP-18, removal of CKR-1, or specific knockdown of CKR-1 in SMD or AIB neurons lead to shallower turns hence less robust escape steering. Consistently, the Ca2+ transients elevation of head motor neuron SMD during escape steering is attenuated upon the removal of NLP-18 or CKR-1. in vitro, synthetic NLP-18 directly evokes CKR-1-dependent currents in oocytes and CKR-1-dependent Ca2+ transients in SMD. Thus, cholecystokinin signaling modulates an escape circuit to generate robust escape steering.

scholarly journals State dependence of neural networks on past history and stimulus presentation in Caenorhabditis elegans

2021 ◽  
Author(s):  
Zachary T. Cecere ◽  
Eviatar Yemini ◽  
Tatyana O. Sharpee ◽  
Sreekanth H. Chalasani
Keyword(s):  
Caenorhabditis Elegans ◽  
Calcium Imaging ◽  
Multinomial Logistic Regression ◽  
Past History ◽  
Stimulus Presentation ◽  
State Dependence ◽  
Global Scale ◽  
Stimulus Onset ◽  
Global Dynamics ◽  
Multinomial Logistic Regression Model

AbstractNeurons cooperate and interact with one another to generate behavior. While individual neurons and their ensembles are often probed in isolation, very little is known about their relationships on a global scale. Here, we define six groups of neurons with interactions that encode and process food stimulus responses in Caenorhabditis elegans. Using brain-wide calcium imaging in restrained animals, we categorized active neurons into either: sensory (On or Off) or motor (forward, backward, dorsal or ventral turn) neuron groups. Next, we show that stimulus onset rapidly shifts activity from backward to forward motor neuron groups, while prolonged stimulus removal is required to initiate converse activity. Finally, we use a gated multinomial logistic regression model to understand how stimulus processing depends on network state. This study shows how neural activity is coordinated across a network to generate global dynamics that drive behavior.

scholarly journals Co-Contraction Analyses of the Body Wall Muscles in Caenorhabditis Elegans by Calcium Imaging Assay and Simulation

2021 ◽  
Author(s):  
Zu Soh ◽  
Hiroki Yamashita ◽  
Michiyo Suzuki ◽  
Kazuma Sakamoto ◽  
Toshio Tsuji
Keyword(s):  
Caenorhabditis Elegans ◽  
Calcium Imaging ◽  
Body Wall ◽  
The Body ◽  
Viscous Drag ◽  
Dynamics Model ◽  
Local Curvature ◽  
Significant Difference ◽  
Body Dynamics ◽  
Body Wall Muscles

Abstract Caenorhabditis elegans can generate locomotion under various environments with completely different drag levels. Therefore, animals should have strategies for adapting to the changes in the dynamics of locomotion imposed by various environments. We hypothesized that co-contraction between the ventral and dorsal body wall muscles plays such a role and validated the presence of a co-contraction strategy through both experimental and mathematical modeling approaches. To this end, the fluorescence of calcium ion (Ca2+) corresponding to a part of activities of the body wall muscles were measured. The results indicated a significant difference in the co-fluorescence rate between the animals moving in low- and high-drag environments. The contribution of co-contraction to the dynamics of locomotion was then analysed using a body dynamics model. The simulation results suggested that co-contraction allows the dominance of body stiffness over viscous drag so that the phase difference between the local curvature of the body and muscle activities can be maintained under different environmental drag levels. Therefore, co-contraction can be an effective strategy for adapting to environmental drag that changes the dynamics of locomotion.

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