scholarly journals Real-time multimodal optical control of neurons and muscles in freely behaving Caenorhabditis elegans

2011 ◽  
Vol 8 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Jeffrey N Stirman ◽  
Matthew M Crane ◽  
Steven J Husson ◽  
Sebastian Wabnig ◽  
Christian Schultheis ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Real Time ◽  
Optical Control ◽  
Freely Behaving

scholarly journals Brevinin-2 Drug Family—New Applied Peptide Candidates Against Methicillin-Resistant Staphylococcus aureus and Their Effects on Lys-7 Expression of Innate Immune Pathway DAF-2/DAF-16 in Caenorhabditis elegans

2018 ◽  
Vol 8 (12) ◽  
pp. 2627
Author(s):  
Hui Xie ◽  
Yonghua Zhan ◽  
Xueli Chen ◽  
Qi Zeng ◽  
Dan Chen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Survival Rate ◽  
Caenorhabditis Elegans ◽  
Antimicrobial Peptides ◽  
Real Time ◽  
Innate Immune ◽  
C Elegans ◽  
New Drug ◽  
Immune Pathway ◽  
Innate Immune Pathway

The issue of Staphylococcus aureus (MRSA) developing a resistance to drugs such as methicillin has long been the focus for new drug development. In recent years, antimicrobial peptides, such as small molecular peptides with broad-spectrum antibacterial activity and special antibacterial mechanism, have shown a strong medicinal potential. In particular, the Brevinin-2 family has been shown to have a significant inhibitory effect against gram-positive bacteria (G+). In this study, we researched the influence of MRSA on the behavior and survival rate of nematodes. We established an assay of Caenorhabditis elegans–MRSA antimicrobial peptides to screen for new potent anti-infective peptides against MRSA. From the Brevinin-2 family, 13 peptides that had shown strong effects on G+ were screened for their ability to prolong the lifespan of infected worms. Real-time Polymerase Chain Reaction (PCR) tests were used to evaluate the effect on the innate immune pathway dauer formation defective (DAF)-2/DAF-16 of C. elegans. The assay successfully screened and filtered out four of the 13 peptides that significantly improved the survival rate of MRSA-infected worms. The result of real-time PCR indicated that the mRNA and protein expression levels of lys-7 were consistently upregulated by being treated with four of the Brevinin-2 family. The Brevinin-2 family peptides, including Brevinin-2, Brevinin-2-OA3, Brevinin-2ISb, and Brevinin-2TSa, also played an active role in the DAF-2/DAF-16 pathway in C. elegans. We successfully demonstrated the utility of anti-infective peptides that prolong the survival rate of the MRSA-infected host and discovered the relationship between antibacterial peptides and the innate immune system of C. elegans. We demonstrated the antimicrobial effects of Brevinin-2 family peptides, indicating their potential for use as new drug candidates against MRSA infections.

scholarly journals Presynaptic MAST kinase controls opposing postsynaptic responses to convey stimulus valence in Caenorhabditis elegans

2020 ◽  
Vol 117 (3) ◽  
pp. 1638-1647 ◽  
Author(s):  
Shunji Nakano ◽  
Muneki Ikeda ◽  
Yuki Tsukada ◽  
Xianfeng Fei ◽  
Takamasa Suzuki ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Diacylglycerol Kinase ◽  
Inhibitory Response ◽  
Thermal Gradients ◽  
Postsynaptic Neuron ◽  
Presynaptic Neuron ◽  
Presynaptic Plasticity ◽  
Freely Behaving ◽  
Stimulus Valence ◽  
Thermal Stimuli

Presynaptic plasticity is known to modulate the strength of synaptic transmission. However, it remains unknown whether regulation in presynaptic neurons can evoke excitatory and inhibitory postsynaptic responses. We report here that the Caenorhabditis elegans homologs of MAST kinase, Stomatin, and Diacylglycerol kinase act in a thermosensory neuron to elicit in its postsynaptic neuron an excitatory or inhibitory response that correlates with the valence of thermal stimuli. By monitoring neural activity of the valence-coding interneuron in freely behaving animals, we show that the alteration between excitatory and inhibitory responses of the interneuron is mediated by controlling the balance of two opposing signals released from the presynaptic neuron. These alternative transmissions further generate opposing behavioral outputs necessary for the navigation on thermal gradients. Our findings suggest that valence-encoding interneuronal activity is determined by a presynaptic mechanism whereby MAST kinase, Stomatin, and Diacylglycerol kinase influence presynaptic outputs.

Paper-Based Analytical Device for Real-Time Monitoring of Egg Hatching in the Model Nematode Caenorhabditis elegans

ACS Sensors ◽  
2020 ◽  
Vol 5 (6) ◽  
pp. 1750-1757
Author(s):  
Julaluk Noiphung ◽  
Mani Iyer Prasanth ◽  
Tewin Tencomnao ◽  
Wanida Laiwattanapaisal
Keyword(s):  
Caenorhabditis Elegans ◽  
Real Time ◽  
Real Time Monitoring ◽  
Nematode Caenorhabditis Elegans ◽  
Egg Hatching ◽  
Analytical Device

Automated imaging of neuronal activity in freely behaving Caenorhabditis elegans

2010 ◽  
Vol 187 (2) ◽  
pp. 229-234 ◽  
Author(s):  
Juliette Ben Arous ◽  
Yoshinori Tanizawa ◽  
Ithai Rabinowitch ◽  
Didier Chatenay ◽  
William R. Schafer
Keyword(s):  
Caenorhabditis Elegans ◽  
Neuronal Activity ◽  
Automated Imaging ◽  
Freely Behaving

scholarly journals Real‐time pharmacokinetic and pharmacodynamic measurements of drugs within the brains of freely behaving rats.

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Kyle L. Ploense ◽  
Phillipe Dauphin-Ducharme ◽  
Netzahualcóyotl Arroyo-Curras ◽  
Serina Williams ◽  
Nitsan Schwarz ◽  
...  
Keyword(s):  
Real Time ◽  
Freely Behaving
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