Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

10.3791/60920 ◽  
2020 ◽  
Author(s):  
Nan Chen ◽  
Kai Zhu ◽  
Shiqiang Yan ◽  
Junmin Li ◽  
Tianyi Pan ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Cell Distribution ◽  
Homogeneous Cell

scholarly journals An optimized 3D-printed perfusion bioreactor for homogeneous cell seeding in bone substitute scaffolds for future chairside applications

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nadja Engel ◽  
Carsten Fechner ◽  
Annika Voges ◽  
Robert Ott ◽  
Jan Stenzel ◽  
...  
Keyword(s):  
In Silico ◽  
Bone Substitute ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Cell Distribution ◽  
Cell Seeding ◽  
Outlet Port ◽  
Short Period ◽  
Homogeneous Cell ◽  
Perfusion Mode

AbstractA clinical implementation of cell-based bone regeneration in combination with scaffold materials requires the development of efficient, controlled and reproducible seeding procedures and a tailor-made bioreactor design. A perfusion system for efficient, homogeneous, and rapid seeding with human adipogenic stem cells in bone substitute scaffolds was designed. Variants concerning medium inlet and outlet port geometry, i.e. cylindrical or conical diffuser, cell concentration, perfusion mode and perfusion rates were simulated in silico. Cell distribution during perfusion was monitored by dynamic [18F]FDG micro-PET/CT and validated by laser scanning microscopy with three-dimensional image reconstruction. By iterative feedback of the in silico and in vitro experiments, the homogeneity of cell distribution throughout the scaffold was optimized with adjustment of flow rates, cell density and perfusion properties. Finally, a bioreactor with a conical diffusor geometry was developed, that allows a homogeneous cell seeding (hoover coefficient: 0.24) in less than 60 min with an oscillating perfusion mode. During this short period of time, the cells initially adhere within the entire scaffold and stay viable. After two weeks, the formation of several cell layers was observed, which was associated with an osteogenic differentiation process. This newly designed bioreactor may be considered as a prototype for chairside application.

scholarly journals Biomimicry in Bio-Manufacturing: Developments in Melt Electrospinning Writing Technology Towards Hybrid Biomanufacturing

2019 ◽  
Vol 9 (17) ◽  
pp. 3540 ◽  
Author(s):  
Ferdows Afghah ◽  
Caner Dikyol ◽  
Mine Altunbek ◽  
Bahattin Koc
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Future Perspective ◽  
Melt Electrospinning ◽  
Wide Range ◽  
Challenges And Opportunities ◽  
Potential Applications ◽  
Homogeneous Cell

Melt electrospinning writing has been emerged as a promising technique in the field of tissue engineering, with the capability of fabricating controllable and highly ordered complex three-dimensional geometries from a wide range of polymers. This three-dimensional (3D) printing method can be used to fabricate scaffolds biomimicking extracellular matrix of replaced tissue with the required mechanical properties. However, controlled and homogeneous cell attachment on melt electrospun fibers is a challenge. The combination of melt electrospinning writing with other tissue engineering approaches, called hybrid biomanufacturing, has introduced new perspectives and increased its potential applications in tissue engineering. In this review, principles and key parameters, challenges, and opportunities of melt electrospinning writing, and particularly, recent approaches and materials in this field are introduced. Subsequently, hybrid biomanufacturing strategies are presented for improved biological and mechanical properties of the manufactured porous structures. An overview of the possible hybrid setups and applications, future perspective of hybrid processes, guidelines, and opportunities in different areas of tissue/organ engineering are also highlighted.

scholarly journals Multidimensional transcriptomics provides detailed information about immune cell distribution and identity in HER2+ breast tumors

2018 ◽  
Author(s):  
Fredrik Salmén ◽  
Sanja Vickovic ◽  
Ludvig Larsson ◽  
Linnea Stenbeck ◽  
Johan Vallon-Christersson ◽  
...  
Keyword(s):  
Tumor Tissue ◽  
Immune Cell ◽  
Three Dimensional ◽  
Cell Infiltration ◽  
Immune Cell Infiltration ◽  
Cell Distribution ◽  
Tissue Sections ◽  
Disease States ◽  
Her2 Breast Cancer ◽  
Transcriptomics Data

AbstractThe comprehensive analysis of tumor tissue heterogeneity is crucial for determining specific disease states and establishing suitable treatment regimes. Here, we analyze tumor tissue sections from ten patients diagnosed with HER2+ breast cancer. We obtain and analyze multidimensional, genome-wide transcriptomics data to resolve spatial immune cell distribution and identity within the tissue sections. Furthermore, we determine the extent of immune cell infiltration in different regions of the tumor tissue, including invasive cancer regions. We combine cross-sectioning and computational alignment to build three-dimensional images of the transcriptional landscape of the tumor and its microenvironment. The three-dimensional data clearly demonstrates the heterogeneous nature of tumor-immune interactions and reveal interpatient differences in immune cell infiltration patterns. Our study shows the potential for an improved stratification and description of the tumor-immune interplay, which is likely to be essential in treatment decisions.

scholarly journals Arylboronate esters mediated self-healable and biocompatible dynamic G-quadruplex hydrogels as promising 3D-bioinks

2018 ◽  
Vol 54 (14) ◽  
pp. 1778-1781 ◽  
Author(s):  
Ankan Biswas ◽  
Sara Malferrari ◽  
Deepak M. Kalaskar ◽  
Apurba K. Das
Keyword(s):  
Molecular Weight ◽  
Cell Viability ◽  
Low Molecular Weight ◽  
Cell Distribution ◽  
High Cell ◽  
G Quadruplex ◽  
Homogeneous Cell

High cell viability and homogeneous cell distribution within extrudable low molecular weight self-healable G-quadruplex hydrogel make it as suitable 3D bioink.

scholarly journals Tenogenesis of Decellularized Porcine Achilles Tendon Matrix Reseeded with Human Tenocytes in the Nude Mice Xenograft Model

2018 ◽  
Vol 19 (7) ◽  
pp. 2059 ◽  
Author(s):  
Anke Lohan ◽  
Benjamin Kohl ◽  
Carola Meier ◽  
Gundula Schulze-Tanzil
Keyword(s):  
Nude Mice ◽  
Xenograft Model ◽  
Scoring Systems ◽  
Hamstring Tendon ◽  
Polyglycolic Acid ◽  
Cell Distribution ◽  
A Cell ◽  
Homogeneous Cell

Cultivation of autologous human tenocytes in a cell-free xenogenic extracellular tendon matrix (xECM) could present an approach for tendon reconstruction. The aim of this study was to achieve tendon-like tissue formation by implanting decellularized porcine Achilles tendons recellularized with human hamstring tendon-derived tenocytes into nude mice. The structure of decellularized xECM was histologically monitored before being dynamically reseeded with human tenocytes. After 6–12 weeks in vivo, construct quality was monitored using macroscopical and histological scoring systems, vitality assay and quantitative DNA and glycosaminoglycan (GAG) assays. For comparison to tendon xECM, a synthetic polyglycolic acid (PGA) polymer was implanted in a similar manner. Despite decellularized xECM lost some GAGs and structure, it could be recellularized in vitro with human tenocytes, but the cell distribution remained inhomogeneous, with accumulations at the margins of the constructs. In vivo, the xECM constructs revealed in contrast to the PGA no altered size, no inflammation and encapsulation and a more homogeneous cell distribution. xECM reseeded with tenocytes showed superior histological quality than cell-free implanted constructs and contained surviving human cells. Their DNA content after six and 12 weeks in vivo resembled that of native tendon and xECM recellularized in vitro. Results suggest that reseeded decellularized xECM formed a tendon-like tissue in vivo.

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