scholarly journals Cell-Laden Poly(ɛ-caprolactone)/Alginate Hybrid Scaffolds Fabricated by an Aerosol Cross-Linking Process for Obtaining Homogeneous Cell Distribution: Fabrication, Seeding Efficiency, and Cell Proliferation and Distribution

2013 ◽  
Vol 19 (10) ◽  
pp. 784-793 ◽  
Author(s):  
HyeongJin Lee ◽  
SeungHyun Ahn ◽  
Lawrence J. Bonassar ◽  
Wook Chun ◽  
GeunHyung Kim
Keyword(s):  
Cell Proliferation ◽  
Cross Linking ◽  
Cell Distribution ◽  
Homogeneous Cell ◽  
Hybrid Scaffolds ◽  
Seeding Efficiency

Hyaluronic Acid Hydrogel Particles Obtained Using Liposomes as Templates

2021 ◽  
Vol 7 (1) ◽  
pp. 7
Author(s):  
Irene Abelenda Núñez ◽  
Ramón G. Rubio ◽  
Francisco Ortega ◽  
Eduardo Guzmán
Keyword(s):  
Cell Proliferation ◽  
Cross Linking ◽  
Hydrogel Particles ◽  
Cell Proliferation And Migration ◽  
Different Types ◽  
And Migration ◽  
Tissue Matrix ◽  
Hyaluronic Acid Hydrogel ◽  
3D Networks ◽  
Proliferation And Migration

Hydrogels (HG) are 3D networks of hydrophilic macromolecules linked by different “cross-linking points”, which have as a main advantage their capacity for the adsorption of large amounts of water without any apparent dissolution. This allows hydrogels to undergo reversible swelling–shrinking processes upon the modification of the environmental conditions (pH, ionic strength or temperature). This stimuli-responsiveness and their ability for entrapping in their interior different types of molecules makes hydrogels suitable platforms for drug delivery applications. Furthermore, HGs exhibit certain similarities to the extracellular tissue matrix and can be used as a support for cell proliferation and migration.

scholarly journals Hybrid gelation processes in enzymatically gelled gelatin: impact on nanostructure, macroscopic properties and cellular response

Soft Matter ◽  
2013 ◽  
Vol 9 (29) ◽  
pp. 6986-6999 ◽  
Author(s):  
Franziska Bode ◽  
Marcelo Alves da Silva ◽  
Paul Smith ◽  
Christian D. Lorenz ◽  
Seth McCullen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cellular Response ◽  
Cross Linking ◽  
Cluster Growth ◽  
Macroscopic Properties ◽  
Physical Gelation ◽  
Triple Helices

Enzymatic cross-linking of gelatin (left) proceeds through cluster growth (red); when combined with physical gelation, clusters are constrained by triple-helices (black), yielding a more ordered and efficient network, favouring cell proliferation.

scholarly journals Active targeting of nanoparticles: An innovative technology for drug delivery in cancer therapeutics

2019 ◽  
Vol 9 (1-s) ◽  
pp. 408-415 ◽  
Author(s):  
Rupalben Kaushalkumar Jani ◽  
Gohil Krupa
Keyword(s):  
Drug Delivery ◽  
Cell Proliferation ◽  
Cancer Therapy ◽  
Targeted Delivery ◽  
Cancer Therapeutics ◽  
Active Targeting ◽  
Innovative Technology ◽  
Cross Linking ◽  
Uncontrolled Cell Proliferation ◽  
Insight Into

In nanomedicines, currently a wide array of reported nanoparticle systems is being explored by targeting schemes which suggests great potential of targeted delivery to revolutionize cancer therapeutics. This review  gives insight into recent  challenges in modification of nanoparticle systems for enhanced cancer therapy  acknowledged by researchers to date and also outlines different major targeting strategies of nanoparticle systems that have been utilized for the delivery of therapeutics or imaging agents, targeting ligand and cross-linking agent to cancer  which was divided into three sections: 1) Angiogenesis associated targeting, 2) Uncontrolled cell proliferation targeting and 3) Tumor cell targeting. Keywords: nanoparticles, tumor cells, active targeting, targeting strategies, targeting ligands

scholarly journals An optimized 3D-printed perfusion bioreactor for homogeneous cell seeding in bone substitute scaffolds for future chairside applications

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nadja Engel ◽  
Carsten Fechner ◽  
Annika Voges ◽  
Robert Ott ◽  
Jan Stenzel ◽  
...  
Keyword(s):  
In Silico ◽  
Bone Substitute ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Cell Distribution ◽  
Cell Seeding ◽  
Outlet Port ◽  
Short Period ◽  
Homogeneous Cell ◽  
Perfusion Mode

AbstractA clinical implementation of cell-based bone regeneration in combination with scaffold materials requires the development of efficient, controlled and reproducible seeding procedures and a tailor-made bioreactor design. A perfusion system for efficient, homogeneous, and rapid seeding with human adipogenic stem cells in bone substitute scaffolds was designed. Variants concerning medium inlet and outlet port geometry, i.e. cylindrical or conical diffuser, cell concentration, perfusion mode and perfusion rates were simulated in silico. Cell distribution during perfusion was monitored by dynamic [18F]FDG micro-PET/CT and validated by laser scanning microscopy with three-dimensional image reconstruction. By iterative feedback of the in silico and in vitro experiments, the homogeneity of cell distribution throughout the scaffold was optimized with adjustment of flow rates, cell density and perfusion properties. Finally, a bioreactor with a conical diffusor geometry was developed, that allows a homogeneous cell seeding (hoover coefficient: 0.24) in less than 60 min with an oscillating perfusion mode. During this short period of time, the cells initially adhere within the entire scaffold and stay viable. After two weeks, the formation of several cell layers was observed, which was associated with an osteogenic differentiation process. This newly designed bioreactor may be considered as a prototype for chairside application.

Tentacular and oral-disc regeneration in the sea anemone, Aiptasia diaphana

Development ◽  
1971 ◽  
Vol 26 (2) ◽  
pp. 253-270
Author(s):  
Irwin I. Singer
Keyword(s):  
Electron Microscopy ◽  
Cell Proliferation ◽  
Cellular Proliferation ◽  
Interstitial Cell ◽  
Interstitial Cells ◽  
Thymidine Uptake ◽  
Cell Distribution ◽  
Oral Disc ◽  
Cellular Division ◽  
Disc Regeneration

Autoradiography with [3H]thymidine and electron microscopy were used to determine (a) the patterns of cellular division exhibited by intact anemones, (b) if measurable increases in cellular proliferation accompany oral-disc regeneration, (c) whether interstitial cells are present in Aiptasia, and (d) if these cells could be responsible for the latter proliferative patterns. An oral-aboral gradient in cellular proliferation was exhibited by the epidermis of uncut anemones, with the highest levels in the tentacles. Wound healing did not require cell proliferation and did not immediately stimulatecellular division which was associated with subsequent morphogenetic events. Indices of presumptive oral-disc [3H]thymidine uptake into nuclei increased tenfold with the outgrowth of the new tentacles. This increase occurred in the epidermis, while only small amounts of gastrodermal proliferation were detected. It is hypothesized that the epidermis contributes new cells to the expanding gastrodermis during tentacle budding. Most of the [3H]thymidine-labeled nuclei were localized in the basal portions of the epidermis of intact anemones and 1- to 2-day-old regenerates; very few gastrodermal nuclei accumulated the label. Nests of interstitial cells and transforming interstitial cells were localized in the exact epidermal regions where nuclear labeling took place, suggesting that the proliferative patterns of intact and regenerating Aiptasia are a function of their interstitial cell distribution.

scholarly journals A Profibrotic Phenotype in Naïve and in Fibrotic Lung Myofibroblasts Is Governed by Modulations in Thy-1 Expression and Activation

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Pazit Y. Cohen ◽  
Raphael Breuer ◽  
Shulamit B. Wallach-Dayan
Keyword(s):  
Cell Proliferation ◽  
Genetic Manipulation ◽  
Cell Mass ◽  
Angiotensin Receptors ◽  
Cross Linking ◽  
Collagen Deposition ◽  
Wild Type ◽  
Abnormal Accumulation ◽  
Free Environment ◽  
Deficient Mice

Lung fibrosis is characterized by abnormal accumulation of Thy-deficient fibroblasts in the interstitium of the alveolar space. We have previously shown in bleomycin-treated chimeric Thy1-deficient mice with wild-type lymphocytes that Thy1-deficient fibroblasts accumulate and promote fibrosis and an “inflammation-free” environment. Here, we aimed to identify the critical effects of Thy1, or the absence of Thy1, in lung myofibroblast profibrotic functions, particularly proliferation and collagen deposition. Using specific Thy1 siRNA in Thy1-positive cells, Thy1 knockout cells, Thy1 cDNA expression vector in Thy1-deficient cells, and Thy1 cross-linking, we evaluated cell proliferation (assessed by cell mass and BrdU uptake), differentiation (using immunofluorescence), and collagen deposition (using Sircol assay). We found that myofibroblast Thy1 cross-linking and genetic manipulation modulate cell proliferation and expression of Fgf (fibroblast growth factor) and Angtl (angiotensin) receptors (using qPCR) that are involved in myofibroblast proliferation, differentiation, and collagen deposition. In conclusion, lung myofibroblast downregulation of Thy1 expression is critical to increase proliferation, differentiation, and collagen deposition.

The Novel Bispecific Diabody αCD40/αCD28 Strengthens Leukemic Dendritic Cell Induced T Cell Reactivity.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3710-3710
Author(s):  
Ilse Houtenbos ◽  
Saskia J.A.M. Santegoets ◽  
Theresia M. Westers ◽  
Quinten Waisfisz ◽  
Sergey Kipriyanov ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cells ◽  
T Cell ◽  
Dendritic Cell ◽  
Cd8 T Cells ◽  
Cluster Formation ◽  
T Cell Proliferation ◽  
Cross Linking ◽  
Bispecific Diabody ◽  
Control Protein

Abstract Dendritic cell (DC)-based immunotherapy faces new challenges since efficacy of DC vaccines in clinical trials has been inconsistent. Strategies to improve immune responses induced by DC are currently being explored. We have recently shown the feasibility of generating fully functional DC from Acute Myeloid Leukemic (AML) blasts, but with varying expression levels of the important costimulatory molecule CD86. To overcome this variability, we developed a novel bispecific diabody (BsDb) simultaneously and agonistically targeting CD40 on AML-DC and CD28 on naïve T cells. Beside optimization of CD28-mediated signaling, the resulting cellular cross-linking was also hypothesized to increase the strength and duration of T cell/AML-DC interactions, thus increasing T cell responsiveness to AML antigens. Indeed the αCD40/αCD28-bispecific diabody provokes increased T cell-DC cluster formation as assessed by light microscopy. Significant increased cluster formation was observed when T cells and AML-DC were cocultured in presence of the BsDb as compared to T cells incubated with a control protein (46%±2 versus 22%±1 respectively, p<0.05). Prior incubation of T cells and/or AML-DC with CD28 or CD40, respectively, completely prevented cluster formation in presence of the BsDb indicating specific binding of the BsDb to CD40 and CD28. The αCD40/αCD28 BsDb significantly increases T cell proliferation induced by AML-DC as compared to the unstimulated cocultures, in a dose dependent manner, as evaluated by mixed lymphocyte reactions (fold increased T cell proliferation of cocultures stimulated with BsDb as compared to unstimulated cocultures:170%±12, p<0.05). In addition, BsDb is capable of DC maturation induction as shown by significant increased mean fluorescence index (MFI) of the maturation markers CD80 (MFI of AML-DC cultured in presence of control protein vs AML-DC cultured in presence of BsDb: 22±5 vs 12±3, p<0.05) and CD83 (4±1 vs 1.5±0.5, p<0.05). In order to determine the effect of aCD40/aCD28-bispecific diabody-mediated cross-linking of AML-derived DC and CD8+ T cells on the induction efficiency of tumor-specific CTL, AML-DC derived from the HLA-A2+ AML cell line MUTZ-3 were pre-incubated with the aCD40/aCD28-bispecific diabody, loaded with the heteroclitic variant of the aa988 epitope of hTERT, and used as stimulator cells in an HLA-A2-matched allogeneic in vitro CTL induction protocol. In total nine parallel bulk cultures, were stimulated twice with peptide-loaded MUTZ-3 DC, either pulsed with control protein or the aCD40/aCD28-bispecific diabody. hTERT988Y-specific CD8+ T cells could be detected in 5/9 individual cultures when stimulated with DC pulsed with the aCD40/aCD28-bispecific diabody, whereas in only 1/9 individual cultures hTERT988Y-specific CD8+ T cells could be detected when stimulated with DC pulsed with the control protein. Thus, priming efficacy of tumor-specific cytotoxic T cells can also be improved by cross-linking AML-DC and T cells with the αCD40/αCD28 diabody. We propose that the αCD40/αCD28-bispecific diabody can serve as a potent therapeutic tool to effectively augment anti-tumor T cell responses elicited by AML-DC.

scholarly journals Arylboronate esters mediated self-healable and biocompatible dynamic G-quadruplex hydrogels as promising 3D-bioinks

2018 ◽  
Vol 54 (14) ◽  
pp. 1778-1781 ◽  
Author(s):  
Ankan Biswas ◽  
Sara Malferrari ◽  
Deepak M. Kalaskar ◽  
Apurba K. Das
Keyword(s):  
Molecular Weight ◽  
Cell Viability ◽  
Low Molecular Weight ◽  
Cell Distribution ◽  
High Cell ◽  
G Quadruplex ◽  
Homogeneous Cell

High cell viability and homogeneous cell distribution within extrudable low molecular weight self-healable G-quadruplex hydrogel make it as suitable 3D bioink.

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