An automated microfluidic platform for calcium imaging of chemosensory neurons in Caenorhabditis elegans

Lab on a Chip ◽  
2010 ◽  
Vol 10 (20) ◽  
pp. 2758 ◽  
Author(s):  
Trushal Vijaykumar Chokshi ◽  
Daphne Bazopoulou ◽  
Nikos Chronis
Keyword(s):  
Caenorhabditis Elegans ◽  
Calcium Imaging ◽  
Microfluidic Platform ◽  
Chemosensory Neurons

Fast whole‐body motor neuron calcium imaging of freely moving Caenorhabditis elegans without coverslip pressed

2021 ◽  
Author(s):  
Haiwen Li ◽  
Fan Feng ◽  
Muyue Zhai ◽  
Jia Zhi Zhang ◽  
Jingyuan Jiang ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Motor Neuron ◽  
Calcium Imaging ◽  
Whole Body ◽  
Freely Moving

Caenorhabditis elegans senses protons through amphid chemosensory neurons

Neuroreport ◽  
2000 ◽  
Vol 11 (10) ◽  
pp. 2229-2232 ◽  
Author(s):  
Yoshihiro Sambongi ◽  
Kenji Takeda ◽  
Tokumitsu Wakabayashi ◽  
Ikuo Ueda ◽  
Yoh Wada ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Chemosensory Neurons

scholarly journals Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

10.3791/56911 ◽  
2018 ◽  
Author(s):  
Bhavya Ravi ◽  
Layla M. Nassar ◽  
Richard J. Kopchock ◽  
Pravat Dhakal ◽  
Michael Scheetz ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Calcium Imaging

scholarly journals A trade-off switch of two immunological memories in Caenorhabditis elegans reinfected by bacterial pathogens

2020 ◽  
Vol 295 (50) ◽  
pp. 17323-17336
Author(s):  
Jinyuan Yan ◽  
Ninghui Zhao ◽  
Zhongshan Yang ◽  
Yuhong Li ◽  
Hua Bai ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Caenorhabditis Elegans ◽  
Pathogenic Bacteria ◽  
Immunological Memory ◽  
Innate Immune ◽  
Microbial Pathogens ◽  
Immune Memory ◽  
Microbial Infection ◽  
Trade Off ◽  
Chemosensory Neurons

Recent studies have suggested that innate immune responses exhibit characteristics associated with memory linked to modulations in both vertebrates and invertebrates. However, the diverse evolutionary paths taken, particularly within the invertebrate taxa, should lead to similarly diverse innate immunity memory processes. Our understanding of innate immune memory in invertebrates primarily comes from studies of the fruit fly Drosophila melanogaster, the generality of which is unclear. Caenorhabditis elegans typically inhabits soil harboring a variety of fatal microbial pathogens; for this invertebrate, the innate immune system and aversive behavior are the major defensive strategies against microbial infection. However, their characteristics of immunological memory remains infantile. Here we discovered an immunological memory that promoted avoidance and suppressed innate immunity during reinfection with bacteria, which we revealed to be specific to the previously exposed pathogens. During this trade-off switch of avoidance and innate immunity, the chemosensory neurons AWB and ADF modulated production of serotonin and dopamine, which in turn decreased expression of the innate immunity-associated genes and led to enhanced avoidance via the downstream insulin-like pathway. Therefore, our current study profiles the immune memories during C. elegans reinfected by pathogenic bacteria and further reveals that the chemosensory neurons, the neurotransmitter(s), and their associated molecular signaling pathways are responsible for a trade-off switch between the two immunological memories.

scholarly journals Hydrogel-droplet microfluidic platform for high-resolution imaging and sorting of early larval Caenorhabditis elegans

Lab on a Chip ◽  
2015 ◽  
Vol 15 (6) ◽  
pp. 1424-1431 ◽  
Author(s):  
Guillaume Aubry ◽  
Mei Zhan ◽  
Hang Lu
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
Microfluidic Device ◽  
High Resolution Imaging ◽  
Microfluidic Platform ◽  
C Elegans ◽  
Resolution Imaging

We present a microfluidic device for high-resolution imaging and sorting of early larval C. elegans. The animals are isolated in droplets and temporarily immobilized for imaging using a reversible hydrogel.

scholarly journals Whole-brain calcium imaging with cellular resolution in freely behaving Caenorhabditis elegans

2015 ◽  
Vol 113 (8) ◽  
pp. E1074-E1081 ◽  
Author(s):  
Jeffrey P. Nguyen ◽  
Frederick B. Shipley ◽  
Ashley N. Linder ◽  
George S. Plummer ◽  
Mochi Liu ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Animal Behavior ◽  
Calcium Imaging ◽  
Large Scale ◽  
Calcium Transients ◽  
Whole Brain ◽  
Cellular Resolution ◽  
Calcium Indicator ◽  
Custom Software ◽  
Freely Behaving

The ability to acquire large-scale recordings of neuronal activity in awake and unrestrained animals is needed to provide new insights into how populations of neurons generate animal behavior. We present an instrument capable of recording intracellular calcium transients from the majority of neurons in the head of a freely behaving Caenorhabditis elegans with cellular resolution while simultaneously recording the animal’s position, posture, and locomotion. This instrument provides whole-brain imaging with cellular resolution in an unrestrained and behaving animal. We use spinning-disk confocal microscopy to capture 3D volumetric fluorescent images of neurons expressing the calcium indicator GCaMP6s at 6 head-volumes/s. A suite of three cameras monitor neuronal fluorescence and the animal’s position and orientation. Custom software tracks the 3D position of the animal’s head in real time and two feedback loops adjust a motorized stage and objective to keep the animal’s head within the field of view as the animal roams freely. We observe calcium transients from up to 77 neurons for over 4 min and correlate this activity with the animal’s behavior. We characterize noise in the system due to animal motion and show that, across worms, multiple neurons show significant correlations with modes of behavior corresponding to forward, backward, and turning locomotion.

scholarly journals In vivo calcium imaging of OFF-responding ASK chemosensory neurons in C. elegans

2009 ◽  
Vol 1790 (8) ◽  
pp. 765-769 ◽  
Author(s):  
Tokumitsu Wakabayashi ◽  
Yukihiro Kimura ◽  
Yusuke Ohba ◽  
Ryota Adachi ◽  
Yoh-ichi Satoh ◽  
...  
Keyword(s):  
Calcium Imaging ◽  
Chemosensory Neurons ◽  
C Elegans ◽  
In Vivo Calcium Imaging
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