scholarly journals Single Cell Transfection in Chick Embryos

10.3791/2133 ◽  
2010 ◽  
Author(s):  
Raz Ben-Yair ◽  
Chaya Kalcheim
Keyword(s):  
Single Cell ◽  
Cell Transfection ◽  
Chick Embryos

scholarly journals Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yu Ting Chow ◽  
Shuxun Chen ◽  
Ran Wang ◽  
Chichi Liu ◽  
Chi-wing Kong ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Transfection

scholarly journals Single-cell transfection

2013 ◽  
Vol 7 (10) ◽  
pp. 762-762
Author(s):  
Rachel Won
Keyword(s):  
Single Cell ◽  
Cell Transfection

scholarly journals Single cell transfection by laser-induced breakdown of an optically trapped gold nanoparticle

2014 ◽  
Author(s):  
Yoshihiko Arita ◽  
Martin Ploschner ◽  
Maciej Antkowiak ◽  
Frank Gunn-Moore ◽  
Kishan Dholakia
Keyword(s):  
Single Cell ◽  
Gold Nanoparticle ◽  
Cell Transfection ◽  
Laser Induced Breakdown ◽  
Optically Trapped

scholarly journals THE DIFFERENTIAL SENSITIVITY OF CULTURED CHICK MESODERMAL CELLS TO ACTINOMYCIN D

1969 ◽  
Vol 40 (1) ◽  
pp. 209-215 ◽  
Author(s):  
J. A. Piper ◽  
N. W. Klein
Keyword(s):  
Cell Culture ◽  
Single Cell ◽  
Cell Cultures ◽  
Actinomycin D ◽  
Cell Types ◽  
Differential Sensitivity ◽  
Chick Embryos ◽  
Macromolecular Synthesis ◽  
Single Cell Culture

Cells were isolated from the somite mesoderm and from the unsegmented (presomite) mesoderm of early chick embryos and exposed to actinomycin D in single cell culture. Actinomycin D inhibited proliferation in cell cultures derived from the unsegmented mesoderm, although the same concentrations of this antibiotic did not inhibit cultures derived from the somite mesoderm. This differential sensitivity parallels the regionally specific necrosis and degeneration observed in the unsegmented mesoderm of intact chick embryos exposed to actinomycin D. In culture, both cell types exhibited approximately the same permeability to labeled actinomycin D and showed comparable inhibition of RNA, DNA, and protein syntheses in the presence of the antibiotic. However, freshly isolated mesodermal cells from the somite region had a higher content of RNA than did cells from the unsegmented region, and the somite cells maintained a higher rate of macromolecular synthesis in untreated cultures.

scholarly journals Single-Cell Transfection by Electroporation Using an Electrolyte/Plasmid-Filled Capillary

2009 ◽  
Vol 81 (10) ◽  
pp. 4060-4067 ◽  
Author(s):  
Manyan Wang ◽  
Owe Orwar ◽  
Stephen G. Weber
Keyword(s):  
Single Cell ◽  
Cell Transfection

scholarly journals Lipoplex-Mediated Single-Cell Transfection via Droplet Microfluidics

Small ◽  
2018 ◽  
Vol 14 (40) ◽  
pp. 1802055 ◽  
Author(s):  
Xuan Li ◽  
Mohammad Aghaamoo ◽  
Shiyue Liu ◽  
Do-Hyun Lee ◽  
Abraham P. Lee
Keyword(s):  
Single Cell ◽  
Droplet Microfluidics ◽  
Cell Transfection

scholarly journals Integrated Electrowetting Nanoinjector for Single Cell Transfection

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Elaheh Shekaramiz ◽  
Ganeshkumar Varadarajalu ◽  
Philip J. Day ◽  
H. Kumar Wickramasinghe
Keyword(s):  
Single Cell ◽  
Cell Transfection

Intercellular relationships during cavitation of aggregates of extraembryonic endoderm cells from gastrulating chick embryos

Development ◽  
1984 ◽  
Vol 83 (1) ◽  
pp. 43-61
Author(s):  
Nadine Milos ◽  
Sara E. Zalik ◽  
Esmond J. Sanders ◽  
Irene M. Ledsham
Keyword(s):  
Single Cell ◽  
Cell Layer ◽  
Light And Electron Microscopy ◽  
Cell Group ◽  
Cell Contact ◽  
Chick Embryos ◽  
Tissue Mass ◽  
Extraembryonic Endoderm ◽  
Cell Shapes

Extraembryonic endoderm cells from gastrulating chick embryos undergo epiboly and change from a multilayered cell group to a single cell layer surrounding the yolk. Single cell suspensions from this cell layer can aggregate in vitro to form aggregates that cavitate. To study the stages of cavitation aggregates were harvested after different times in culture, and fixed and processed for light and electron microscopy. In aggregates harvested at 75 min of culture cell contact consisted of areas of parallel and close membrane apposition and interdigitation. Desmosomes were occasionally observed. Aggregates in the early stages of cavitation (24 h) contained numerous intercellular spaces bordered by irregularly shaped cells which appeared to be digesting their yolk and releasing material extracellularly. Long cytoplasmic projections were extended into these spaces. In addition to regions of parallel membrane apposition and interdigitation, desmosomes and adherens junctions were observed. Cells closer to the periphery of the aggregates displayed fewer cell projections and also showed signs of release of material extracellularly. After 48 h of culture, a single smooth-walled central cavity was present and cells still exhibited signs of extracellular release of material. These same cell shapes and intercellular junctions were also observed when area opaca tissue dissected from gastrulating embryos was examined. Aggregates of different sizes were created and cultured. The results suggest that a critical tissue mass may be important for cavitation.

Characterization of a Magnetic Microactuator for Manipulating Nanoparticles in Gene Delivery Applications

2010 ◽  
Author(s):  
Eva Flick ◽  
Wenzhong Li ◽  
Jonas Norpoth ◽  
Christian Jooss ◽  
Gustav Steinhoff ◽  
...  
Keyword(s):  
Thin Film ◽  
Magnetic Nanoparticles ◽  
Gene Delivery ◽  
Single Cell ◽  
Magnetic Force ◽  
Cell Transfection ◽  
Micro Actuator ◽  
Thin Film Fabrication ◽  
Film Fabrication

Magnetically driven gene delivery provides a valuable approach for a single cell transfection. Therapeutic genes can be manipulated by conjugating them to magnetic nanoparticles. The magnetic force required for the manipulation of such complexes can be provided by an electromagnetic micro-actuator designed as micro pole row. The analytical calculations, simulations, and the thin-film fabrication of this micro pole row were carried out previously [1]. This paper presents characterization results of the first microactuator fabrication batch.

scholarly journals Single-cell transfection technologies for cell therapies and gene editing

2020 ◽  
Author(s):  
Bastien Duckert ◽  
Steven Vinkx ◽  
Dries Braeken ◽  
Maarten Fauvart
Keyword(s):  
Single Cell ◽  
Gene Editing ◽  
Cell Transfection ◽  
Cell Therapies
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