scholarly journals Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yu Ting Chow ◽  
Shuxun Chen ◽  
Ran Wang ◽  
Chichi Liu ◽  
Chi-wing Kong ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Transfection

scholarly journals Single-cell transfection

2013 ◽  
Vol 7 (10) ◽  
pp. 762-762
Author(s):  
Rachel Won
Keyword(s):  
Single Cell ◽  
Cell Transfection

scholarly journals Single cell transfection by laser-induced breakdown of an optically trapped gold nanoparticle

2014 ◽  
Author(s):  
Yoshihiko Arita ◽  
Martin Ploschner ◽  
Maciej Antkowiak ◽  
Frank Gunn-Moore ◽  
Kishan Dholakia
Keyword(s):  
Single Cell ◽  
Gold Nanoparticle ◽  
Cell Transfection ◽  
Laser Induced Breakdown ◽  
Optically Trapped

scholarly journals Single-Cell Transfection by Electroporation Using an Electrolyte/Plasmid-Filled Capillary

2009 ◽  
Vol 81 (10) ◽  
pp. 4060-4067 ◽  
Author(s):  
Manyan Wang ◽  
Owe Orwar ◽  
Stephen G. Weber
Keyword(s):  
Single Cell ◽  
Cell Transfection

scholarly journals Lipoplex-Mediated Single-Cell Transfection via Droplet Microfluidics

Small ◽  
2018 ◽  
Vol 14 (40) ◽  
pp. 1802055 ◽  
Author(s):  
Xuan Li ◽  
Mohammad Aghaamoo ◽  
Shiyue Liu ◽  
Do-Hyun Lee ◽  
Abraham P. Lee
Keyword(s):  
Single Cell ◽  
Droplet Microfluidics ◽  
Cell Transfection

scholarly journals Integrated Electrowetting Nanoinjector for Single Cell Transfection

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Elaheh Shekaramiz ◽  
Ganeshkumar Varadarajalu ◽  
Philip J. Day ◽  
H. Kumar Wickramasinghe
Keyword(s):  
Single Cell ◽  
Cell Transfection

Characterization of a Magnetic Microactuator for Manipulating Nanoparticles in Gene Delivery Applications

2010 ◽  
Author(s):  
Eva Flick ◽  
Wenzhong Li ◽  
Jonas Norpoth ◽  
Christian Jooss ◽  
Gustav Steinhoff ◽  
...  
Keyword(s):  
Thin Film ◽  
Magnetic Nanoparticles ◽  
Gene Delivery ◽  
Single Cell ◽  
Magnetic Force ◽  
Cell Transfection ◽  
Micro Actuator ◽  
Thin Film Fabrication ◽  
Film Fabrication

Magnetically driven gene delivery provides a valuable approach for a single cell transfection. Therapeutic genes can be manipulated by conjugating them to magnetic nanoparticles. The magnetic force required for the manipulation of such complexes can be provided by an electromagnetic micro-actuator designed as micro pole row. The analytical calculations, simulations, and the thin-film fabrication of this micro pole row were carried out previously [1]. This paper presents characterization results of the first microactuator fabrication batch.

scholarly journals Single-cell transfection technologies for cell therapies and gene editing

2020 ◽  
Author(s):  
Bastien Duckert ◽  
Steven Vinkx ◽  
Dries Braeken ◽  
Maarten Fauvart
Keyword(s):  
Single Cell ◽  
Gene Editing ◽  
Cell Transfection ◽  
Cell Therapies

Single cell transfection using plasmid decorated AFM probes

2007 ◽  
Vol 355 (3) ◽  
pp. 632-636 ◽  
Author(s):  
Charles M. Cuerrier ◽  
Réjean Lebel ◽  
Michel Grandbois
Keyword(s):  
Single Cell ◽  
Cell Transfection

scholarly journals Single cell optical transfection

2010 ◽  
Vol 7 (47) ◽  
pp. 863-871 ◽  
Author(s):  
David J. Stevenson ◽  
Frank J. Gunn-Moore ◽  
Paul Campbell ◽  
Kishan Dholakia
Keyword(s):  
Plasma Membrane ◽  
Single Cell ◽  
Laser Field ◽  
Small Interfering Rna ◽  
Eukaryotic Cell ◽  
Optical Injection ◽  
Cell Transfection ◽  
Gene Gun ◽  
Transfection Method ◽  
Interfering Rna

The plasma membrane of a eukaryotic cell is impermeable to most hydrophilic substances, yet the insertion of these materials into cells is an extremely important and universal requirement for the cell biologist. To address this need, many transfection techniques have been developed including viral, lipoplex, polyplex, capillary microinjection, gene gun and electroporation. The current discussion explores a procedure called optical injection, where a laser field transiently increases the membrane permeability to allow species to be internalized. If the internalized substance is a nucleic acid, such as DNA, RNA or small interfering RNA (siRNA), then the process is called optical transfection. This contactless, aseptic, single cell transfection method provides a key nanosurgical tool to the microscopist—the intracellular delivery of reagents and single nanoscopic objects. The experimental possibilities enabled by this technology are only beginning to be realized. A review of optical transfection is presented, along with a forecast of future applications of this rapidly developing and exciting technology.

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