scholarly journals Lipoplex-Mediated Single-Cell Transfection via Droplet Microfluidics

Small ◽  
2018 ◽  
Vol 14 (40) ◽  
pp. 1802055 ◽  
Author(s):  
Xuan Li ◽  
Mohammad Aghaamoo ◽  
Shiyue Liu ◽  
Do-Hyun Lee ◽  
Abraham P. Lee
Keyword(s):  
Single Cell ◽  
Droplet Microfluidics ◽  
Cell Transfection

scholarly journals Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yu Ting Chow ◽  
Shuxun Chen ◽  
Ran Wang ◽  
Chichi Liu ◽  
Chi-wing Kong ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Transfection

scholarly journals Massively parallel, time-resolved single-cell RNA sequencing with scNT-Seq

2019 ◽  
Author(s):  
Qi Qiu ◽  
Peng Hu ◽  
Kiya W. Govek ◽  
Pablo G. Camara ◽  
Hao Wu
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Temporal Dynamics ◽  
Embryonic Stem ◽  
Droplet Microfluidics ◽  
Massively Parallel ◽  
Specific Gene ◽  
Time Resolved ◽  
Single Cell Rna Sequencing ◽  
Rna Biogenesis

ABSTRACTSingle-cell RNA sequencing offers snapshots of whole transcriptomes but obscures the temporal dynamics of RNA biogenesis and decay. Here we present single-cell new transcript tagging sequencing (scNT-Seq), a method for massively parallel analysis of newly-transcribed and pre-existing RNAs from the same cell. This droplet microfluidics-based method enables high-throughput chemical conversion on barcoded beads, efficiently marking metabolically labeled newly-transcribed RNAs with T-to-C substitutions. By simultaneously measuring new and old transcriptomes, scNT-Seq reveals neuronal subtype-specific gene regulatory networks and time-resolved RNA trajectories in response to brief (minutes) versus sustained (hours) neuronal activation. Integrating scNT-Seq with genetic perturbation reveals that DNA methylcytosine dioxygenases may inhibit stepwise transition from pluripotent embryonic stem cell state to intermediate and totipotent two-cell-embryo-like (2C-like) states by promoting global RNA biogenesis. Furthermore, pulse-chase scNT-Seq enables transcriptome-wide measurements of RNA stability in rare 2C-like cells. Time-resolved single-cell transcriptomic analysis thus opens new lines of inquiry regarding cell-type-specific RNA regulatory mechanisms.

Inertial-ordering-assisted droplet microfluidics for high-throughput single-cell RNA-sequencing

Lab on a Chip ◽  
2018 ◽  
Vol 18 (5) ◽  
pp. 775-784 ◽  
Author(s):  
Hui-Sung Moon ◽  
Kwanghwi Je ◽  
Jae-Woong Min ◽  
Donghyun Park ◽  
Kyung-Yeon Han ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
High Throughput ◽  
Droplet Microfluidics ◽  
Single Cell Rna Sequencing ◽  
Spiral Microchannel

We developed a modified high-throughput droplet barcoding technique for single-cell Drop-Seq via introduction of hydrodynamic ordering in a spiral microchannel.

scholarly journals Diamagnetic droplet microfluidics applied to single-cell sorting

AIP Advances ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 075106 ◽  
Author(s):  
Stephanie Buryk-Iggers ◽  
Jennifer Kieda ◽  
Scott S. H. Tsai
Keyword(s):  
Single Cell ◽  
Cell Sorting ◽  
Droplet Microfluidics

scholarly journals Single-cell transfection

2013 ◽  
Vol 7 (10) ◽  
pp. 762-762
Author(s):  
Rachel Won
Keyword(s):  
Single Cell ◽  
Cell Transfection
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