scholarly journals THE DIFFERENTIAL SENSITIVITY OF CULTURED CHICK MESODERMAL CELLS TO ACTINOMYCIN D

1969 ◽  
Vol 40 (1) ◽  
pp. 209-215 ◽  
Author(s):  
J. A. Piper ◽  
N. W. Klein
Keyword(s):  
Cell Culture ◽  
Single Cell ◽  
Cell Cultures ◽  
Actinomycin D ◽  
Cell Types ◽  
Differential Sensitivity ◽  
Chick Embryos ◽  
Macromolecular Synthesis ◽  
Single Cell Culture

Cells were isolated from the somite mesoderm and from the unsegmented (presomite) mesoderm of early chick embryos and exposed to actinomycin D in single cell culture. Actinomycin D inhibited proliferation in cell cultures derived from the unsegmented mesoderm, although the same concentrations of this antibiotic did not inhibit cultures derived from the somite mesoderm. This differential sensitivity parallels the regionally specific necrosis and degeneration observed in the unsegmented mesoderm of intact chick embryos exposed to actinomycin D. In culture, both cell types exhibited approximately the same permeability to labeled actinomycin D and showed comparable inhibition of RNA, DNA, and protein syntheses in the presence of the antibiotic. However, freshly isolated mesodermal cells from the somite region had a higher content of RNA than did cells from the unsegmented region, and the somite cells maintained a higher rate of macromolecular synthesis in untreated cultures.

A study of changes in flocculence in a single cell culture of a strain of Saccharomyces cerevisiae

1963 ◽  
Vol 157 (967) ◽  
pp. 223-233 ◽  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Culture ◽  
Liquid Medium ◽  
Single Cell ◽  
Cell Cultures ◽  
Single Cells ◽  
Selective Advantage ◽  
Brewing Yeast ◽  
Flocculent Yeast ◽  
Single Cell Culture

A culture from a single cell of a flocculent brewing yeast became less flocculent during sub-culture on agar slopes. It was found that the culture had become a mixture of a number of variants differing in degree of flocculence. Five of the variants were studied using a sedi­-mentation method to measure flocculence. During subculture on agar and in liquid medium, single cell cultures of all these variants except the least flocculent gave rise to other variants. The proportion of less flocculent yeast increased during further subculture. The change was faster in liquid medium than on agar slopes. Yeast with more stable flocculence could be selected from the part of the population which did not change. Experiments with mixtures of ‘stable’ yeast indicated that the less flocculent yeast had a selective advantage, and that the mechanism of the selection involves the different physical properties of the yeast. All variants examined had the same rate of growth. Cultures derived from single cells of a given variant tended to give rise to the same new variants. Yeast with flocculence similar to the original strains could be isolated from the variant strains. It was not possible to determine whether these variants were produced by gene mutation since spore formation has never been detected in this yeast.

scholarly journals Rapid and Simple Cryopreservation of Anaerobic Ammonium-Oxidizing Bacteria

2012 ◽  
Vol 78 (8) ◽  
pp. 3010-3013 ◽  
Author(s):  
Kim Heylen ◽  
Katharina Ettwig ◽  
Ziye Hu ◽  
Mike Jetten ◽  
Boran Kartal
Keyword(s):  
Cell Culture ◽  
Single Cell ◽  
Anammox Bacteria ◽  
Content Type ◽  
Growth Recovery ◽  
Activity Recovery ◽  
Simple Protocol ◽  
Single Cell Culture ◽  
Preservation Conditions

ABSTRACTA quick and simple protocol for long-term cryopreservation of anaerobic ammonium-oxidizing bacteria (anammox bacteria) was developed. After 29 weeks of preservation at −80°C, activity recovery for all tested cultures under at least one of the applied sets of preservation conditions was observed. Growth recovery was also demonstrated for a single-cell culture of “CandidatusKuenenia stuttgartiensis.”

scholarly journals Tracing blastomere fate choices of early embryos in single cell culture

2010 ◽  
Author(s):  
Mingyou Li ◽  
Jianxin Song ◽  
Yan Yan ◽  
Yongming Yuan ◽  
Chang Ming Li ◽  
...  
Keyword(s):  
Cell Culture ◽  
Single Cell ◽  
Early Embryos ◽  
Single Cell Culture

Growth characteristics in cell culture and pathogenicity in mice of two terrestrial rabies strains indigenous to Canada

1988 ◽  
Vol 34 (1) ◽  
pp. 19-23 ◽  
Author(s):  
W. A. Webster ◽  
K. M. Charlton ◽  
G. A. Casey
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Culture Supernatant ◽  
Neuroblastoma Cell ◽  
Cell Types ◽  
Kidney Cells ◽  
Baby Hamster Kidney Cell ◽  
Baby Hamster Kidney ◽  
The Brain ◽  
Baby Hamster Kidney Cells

Two strains of street rabies virus from striped skunks (Mephitis mephitis) were used to infect either a murine neuroblastoma (NA 1300) or a baby hamster kidney (BHK-21/C13) cell culture and the cell infection rates were noted during 4 days postinfection. These cultures were then passaged for four consecutive passages, and the viruses obtained in the supernatant fluids of passage 4 were then treated as original isolates and used to infect both neuroblastoma and baby hamster kidney cells. The mortality period in Swiss white mice caused by the various virus suspensions was noted. The virus strain from the brain of skunks from Saskatchewan infected neuroblastoma and baby hamster kidney cells equally well, produced similar virus titres in supernatant fluids after four subcultures in both cell types, and appeared to produce similar mortality periods in mice from either the original brain tissue or from cell culture supernatant fluids. On the other hand, the virus from the brains of skunks from Ontario readily infected neuroblastoma but poorly infected baby hamster kidney cell cultures. Passage of this strain through four subcultures in both cell types produced virus titres in the supernatant fluids of equal magnitude. However, reisolation of the virus from the supernatant fluid of passage 4 in neuroblastoma cell cultures showed a similar pattern to that from the original brain, while the virus from baby hamster kidney cell passage supernatant fluid was considerably altered. Although the mortality period in mice was similar with virus from the brain and neuroblastoma cell cultures, this period was shortened when mice were inoculated with baby hamster kidney culture supernatant virus. Virus from the salivary glands of Ontario skunks readily infected both cell types, producing similar titres at 4 days postinfection. The mortality period of mice inoculated with salivary gland suspensions was shorter than of those inoculated with brain suspensions. These findings demonstrate differences in rabies street virus strains that may have affected diagnostic procedures.

scholarly journals Improved single-cell culture achieved using micromolding in capillaries technology coupled with poly (HEMA)

2015 ◽  
Vol 9 (4) ◽  
pp. 044106 ◽  
Author(s):  
Fang Ye ◽  
Jin Jiang ◽  
Honglong Chang ◽  
Li Xie ◽  
Jinjun Deng ◽  
...  
Keyword(s):  
Cell Culture ◽  
Single Cell ◽  
Single Cell Culture ◽  
Micromolding In Capillaries
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