scholarly journals The estimation of efficiency of diet therapy in atopic dermatitis and food allergy in children

2012 ◽  
Vol 9 (4) ◽  
pp. 72-78
Author(s):  
V A Revyakina ◽  
K G Sharapova ◽  
E YU Berezina ◽  
T B Sentsova ◽  
O YU Monosova ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Food Allergy ◽  
Milk Protein ◽  
Goat Milk ◽  
Serum Levels ◽  
Diet Therapy ◽  
Cow Milk ◽  
Milk Formula ◽  
Igg Antibodies ◽  
Positive Skin

Background. Clinical and immunological estimation of efficiency of different special formulas in children with atopic dermatitis due to food allergy. Methods. Fifty four children with atopic dermatitis aged 0,5—3 years old were examined. Children were divided into three groups on the base of their diet therapy. The 1 st group included 15 patients on the hypoallergenic milk-free diet, the 2 nd group (18 patients) was fed by goat milk formula, and the 3 rd group (21 children) was fed by special formula with high hydrolyzed protein. The dynamics of serum levels of IgE and IgG antibodies to cow milk protein and its fractions, and dynamics of cytokine levels (IL-4, IL-5, and IL-10) were measured during different types of diet therapy. Results. The positive skin changes during diet therapy included decrease of erythema, exudation and itching, gradual regression of rash. In 3 months of therapy complete clinical remission was achieved in 10 (66,7%) children in the 1 st group, in 14 (77,8%) children in the 2 nd group and in 15 (71,7%) children in the 3 rd group. The significant decrease of levels of IgG antibodies levels to cow milk protein and its fractions in children during different elimination diets was determined. The significant decrease of levels of specific IgE antibodies to cow milk protein (p

scholarly journals Preexposure prophylaxis of infants’ food allergy

2013 ◽  
Vol 10 (2) ◽  
pp. 37-42
Author(s):  
S N Denisova ◽  
M Yu Belitskaya ◽  
T B Sentsova ◽  
V A Revyakina ◽  
O O Kirillova ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Breast Feeding ◽  
Milk Protein ◽  
Goat Milk ◽  
Diet Therapy ◽  
Cow Milk ◽  
Igg Antibodies ◽  
Mother And Child ◽  
Nursing Mothers ◽  
Cow Milk Protein Allergy

Background. Till the present time the problem of food allergy remains to be the topical subject and demands further development of therapeutical and prevention programs, including diet therapy for nursing mothers. The work is aimed at clinical and immunological evaluating of diet therapy of mothers whose children have atopic dermatitis on the back of breast feeding only. Methods. There were 100 «mother and child» couples kept under observance, that were divided into two groups. All the children had atopic dermatitis (AD), associated to cow milk protein allergy. Nursing mothers from both the groups were treated by diet therapy according to which the cow milk in diet have been replaced either with goat milk of New Zealand origin (first group had 43 «mother and child» couples) or with nondairy products (second group had 57 «mother and child» couples). Effectiveness of diet therapy of nursing mothers has been evaluated by the dynamic of clinical and immunological symptoms of atopic dermatitis in 1-3 months from treatment beginning. Immunological effectiveness of the treatment has been evaluated by the dynamic of indexes of specific IgE and IgG antibodies to cow milk protein, β-lactoglobulin, casein, soy protein and goat milk protein and cytokines levels. Results. In the course of treatment, that included diet therapy of nursing mothers and antiallergic cure of infants, both the groups showed remission and saved breast feeding. Immunological evaluating of therapeutic intervention’s effectiveness revealed the improvement of total IgE, allergenspecific IgE and IgG antibodies to cow milk protein and its fractions, as well as to soy and goat milk protein in blood of all the children on the back of breast feeding. Moreover, complex therapy conduced decreasing of pro-inflammatory cytokines concentration in blood. Conclusion. These findings prove reasonability of dietary treatement for nursing mothers, whose children have atopic dermatitis, associated associated to cow milk protein allergy.

scholarly journals The dynamics of soluble apoptosis markers during diet therapy in infants with atopic dermatitis

2012 ◽  
Vol 9 (3) ◽  
pp. 18-22
Author(s):  
T B Sentsova ◽  
V A Revyakina ◽  
B S Kaganov ◽  
S N Denisova ◽  
I V Vorozhko ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Milk Protein ◽  
Goat Milk ◽  
Diet Therapy ◽  
Cow Milk ◽  
Control Group ◽  
Milk Formula ◽  
Caspase 8 ◽  
Caspase 9 ◽  
Apoptosis Markers

Background. To estimate the dynamics of soluble apoptosis markers in infants with atopic dermatitis for updating mechanisms of immunopathogenesis and improvement of diet therapy. Methods. We observed 66 bottle-fed infants aged 1,5—12 months old (boys -47, girls — 19) with atopic dermatitis (AD). The sensibilization to cow milk protein was revealed in all 66 infants. Detected allergen-specific IgG and IgE antibodies to cow milk protein, its fraction and goat milk protein were the reason to include infants into the 1st group and feed with hydrolyzed formula (27 infants). 39 infants in the 2nd group, who were not sensibilized to goat milk protein, were fed by goat milk based formula. Serum levels of soluble apoptosis markers (sCD153, caspase-8, sFas-L, caspase-9 and annexin-5) were measured by immunoenzyme method (ELISA). Results. The activation of signal apoptosis systems in infants with AD with increased levels of sFas-L и sCD153 was revealed. Levels of caspase-8 and caspase-9 were significantly lower than in control group, and reflected the impaired elimination of modified immmunocompetent cells. The level of annexin-5 was significantly lower in infants with AD than in control group. The estimation of the dynamics of investigated parameters during diet therapy showed significant increase of caspase-9 level in both groups. The level of caspase-8 was increased only in infants who were fed by goat milk formula. Levels of sFas-L, sCD153 and annexin-5 during diet treatment did not differ significantly between groups. Conclusion. The results showed that sCD153, caspase-8, sFas-L, caspase-9 and annexin-5 play a role in the realization of allergic inflammation in infants with AD. The diet therapy with goat milk formula promotes more physiological repair of the effectory component of the apoptosis.

scholarly journals Immunological biomarkers changes during diet therapy in children with food allergy

2015 ◽  
Vol 12 (1) ◽  
pp. 73-78
Author(s):  
I V Vorozhko ◽  
T B Sentsova ◽  
V A Revyakina ◽  
O O Chernyak ◽  
S N Denisova
Keyword(s):  
Food Allergy ◽  
Milk Protein ◽  
Goat Milk ◽  
Specific Ige ◽  
Diet Therapy ◽  
Cow’S Milk ◽  
Igg Antibodies ◽  
Cow's Milk ◽  
Cow’S Milk Protein ◽  
Different Sources

Immunological estimating of different diet therapy types became currently very important. The aim of this work was to study the changes of immunological biomarkers during usage of formulas based on different sources of protein in children with food allergy and to assess and predict the effectiveness of diet therapy. The study included 66 children with food allergy in age from 1,5 months to 1,5 years. Observed children were divided into three groups depending on the type of formulas used. The dynamics of immunoregulatory substances (IL-5, TGF β 1, IL-10, IL-4, IL-2, IL-13, Eotaxin3), and allergenspecific IgE and IgG antibodies to cow’s milk protein, α-lactoglobulin, β-laktoglobulin, casein, soy and goat’s milk during diet therapy were evaluated. Observation duration was 28-30 days. The differences in the dynamics of the investigated parameters depending on the type of formula were revealed. Thus, the use of formula based on cow’s milk hydrolysates revealed their primarily effect on cytokines IL-2, IL-5 and growth factor TGF β ; the use of formula based on soy protein revealed combined effect on humoral immunity (allergen specific IgE and IgG antibodies) and IL-5; the use of formula based on goat milk characterized decreased levels of allergenspecific IgG antibodies to the cow’s milk protein and its fractions, reduced allergen specific IgE antibody concentrations to cow’s milk protein and α-lactoglobulin, decreased levels of IL-5, and, exclusive for this formula - reduction of IL-4 level. Accordingly, the assessment of immunological biomarkers can be used to personalize diet therapy in children with food allergy.

scholarly journals A2 Bovine Milk and Caprine Milk as a Means of Remedy for Milk Protein Allergy

Dairy ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 191-201
Author(s):  
Young W. Park ◽  
George F. W. Haenlein
Keyword(s):  
Milk Protein ◽  
Bovine Milk ◽  
Goat Milk ◽  
Food Sources ◽  
Cow Milk ◽  
Cow’S Milk ◽  
Dairy Goat ◽  
Cow's Milk ◽  
Caprine Milk ◽  
Low Levels

A new type of cow’s milk, called A2 milk, has appeared in the dairy aisles of supermarkets in recent years. Cows’ milk generally contains two major types of beta-casein as A1 and A2 types, although there are 13 genetic variants of β-casein: A1, A2, A3, A4, B, C, D, E, F, H1, H2, I and G. Studies have shown that A1 β-casein may be harmful, and A2 β-casein is a safer choice for human health especially in infant nutrition and health. The A2 cow milk is reportedly easier to digest and better absorb than A1 or other types of milk. The structure of A2 cow’s milk protein is more comparable to human breast milk, as well as milk from goats, sheep and buffalo. Digestion of A1 type milk produces a peptide called β-casomorphin-7 (BCM-7), which is implicated with adverse gastrointestinal effects on milk consumption. In addition, bovine milk contains predominantly αs1-casein and low levels or even absent in αs2-casein, whereby caprine milk has been recommended as an ideal substitute for patients suffering from allergies against cow milk protein or other food sources. Since goat milk contains relatively low levels of αs1-casein or negligible its content, and αs2-casein levels are high in the milk of most dairy goat breeds, it is logical to assume that children with a high milk sensitivity to αs1-casein should tolerate goat milk well. Cow milk protein allergy (CMPA) is considered a common milk digestive and metabolic disorder or allergic disease with various levels of prevalence from 2.5% in children during the first 3 years of life to 12–30% in infants less than 3 months old, and it can go up to even as high as 20% in some countries. CMPA is an IgE-mediated allergy where the body starts to produce IgE antibodies against certain protein (allergens) such as A1 milk and αs1-casein in bovine milk. Studies have shown that ingestion of β-casein A1 milk can cause ischemic heart disease, type-1 diabetes, arteriosclerosis, sudden infant death syndrome, autism, schizophrenia, etc. The knowledge of bovine A2 milk and caprine αs2-casein has been utilized to rescue CMPA patients and other potential disease problems. This knowledge has been genetically applied to milk production in cows or goats or even whole herds of the two species. This practice has happened in California and Ohio, as well as in New Zealand, where this A2 cow milk has been now advanced commercially. In the USA, there have been even promotions of bulls, whose daughters have been tested homozygous for the A2 β-casein protein.

scholarly journals Development of QCM Biosensor with Specific Cow Milk Protein Antibody for Candidate Milk Adulteration Detection

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Setyawan P. Sakti ◽  
Nur Chabibah ◽  
Senja P. Ayu ◽  
Masdiana C. Padaga ◽  
Aulanni’am Aulanni’am
Keyword(s):  
Milk Protein ◽  
Protein A ◽  
Goat Milk ◽  
Specific Protein ◽  
Cow Milk ◽  
Frequency Change ◽  
Milk Product ◽  
Reaction Cell ◽  
Buffer Solution ◽  
Milk Adulteration

Adulteration of goat milk is usually done using cow’s milk product. Cow milk is used as it is widely available and its price is cheaper compared to goat milk. This paper shows a development of candidate tools for milk adulteration using cow milk. A quartz crystal microbalance immunosensor was developed using commercial crystal resonator and polyclonal antibody specific to cow milk protein. A specific protein at 208 KDa is found only in cow milk and does not exist in goat milk. The existence of this protein can be used as an indicator of cow milk content in a target solution. To detect the PSS 208 kDa protein, antibody specific to the PSS 208 was developed. The purified antibody was immobilized on top of the sensor surface on a polystyrene layer. The fraction of the immobilized antibody on the sensor was found at 1.5% of the given antibody. Using a static reaction cell, the developed immunosensor could detect the specific cow milk protein in buffer solution. The detection limit is 1 ppm. A linear relationship between frequency change and specific protein of cow milk concentration is found from a concentration of 1 ppm to 120 ppm.

scholarly journals SAT-076 Hypophosphatemic Rickets Secondary to Elemental Formula Use - Report on Two Cases

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Délia Oliveira Braz ◽  
Renata Santarem Oliveira ◽  
Fernanda Sousa Cardoso Lopes ◽  
Lara Pereira ◽  
Alessandra Domingues ◽  
...  
Keyword(s):  
Gastrointestinal Symptoms ◽  
Serum Levels ◽  
Hypophosphatemic Rickets ◽  
Extremely Low Birth Weight ◽  
Cow Milk ◽  
Milk Formula ◽  
Metabolic Disturbances ◽  
Normal Range ◽  
Elemental Formula ◽  
Phosphate Supplementation

Abstract Background: Hypophosphatemic rickets (HR) is usually an inherited disorder, but it may also occur in several clinical settings as an acquired condition due to phosphate absorption and internal distribution issues. Recently it was described the association between the use of the elemental amino-acid based formula (AAF) and HR. Herein we report two male twins presenting this condition. Clinical case: Two monozygotic preterm (28 weeks + 5 days) brothers, born with extremely low birth weight (895 and 995 grams, -1,4 SDS and -0.9 SDS, respectively), received total parental nutrition from the 1st to the 7th day of life. Afterwards they started oral diet with milk and human milk fortifier FM85 for preventing metabolic bone disease of prematurity. After some weeks they developed abdominal distension, vomiting and hematochezia. Allergy to cow milk protein was suspected and the infants started receiving extensively hydrolyzed milk formula. So, FM85 was suspended and they were put on tricalcium phosphate (12.9%) supplementation from the 4th week of life on. As the gastrointestinal symptoms persisted, the formula was exchanged for elemental formula Neocate at 2 months of age, with improvement of those symptoms. After the 5th week of life the patients developed hypophosphatemia (2.8 mg/dL, reference 4.8-7.4 mg/dL), hyperphosphatasemia (1,619 IU/L and 2,173 IU/L; reference 70-350 IU/L) and low urinary phosphate excretion, keeping normal calcium and PTH ser um levels. Radiographic skeletal inventory showed under mineralized bones with irregular metaphyseal margins, but no signs of fractures. Calcium and phosphate supplementation doses were increased in attempt to correct the metabolic disturbances, and calcitriol was also started. Nevertheless, hypophosphatemia, hyperphosphatasemia and hypophosphaturia persisted. At 4 months of age, Neocate was switched to Alfamino, still an AAF. After that, phosphate serum levels went up until normalization and alkaline phosphatase started to decrease. Calcium and phosphate supplementation were decreased to keep their serum levels at the normal range for age. The boys were then discharged from hospital when they were 4 months old (45 days of life of corrected age), but the family could not afford Alfamino, switching back to Neocate. After that their phosphate serum levels went below normal range again. Conclusion: To our knowledge, this is the first report on monozygotic twins presenting AAF Neocate-related HR. The underlying mechanisms of Neocate induced hypophosphatemia is still elusive, since its content of phosphate and calcium:phosphate ratio are similar to other AAFs. These cases reinforce the importance of evaluating phosphate metabolism in infants receiving AAF while the pathophysiology of this condition is not entirely understood.

scholarly journals UTILIZAÇÃO DE BETACICLODEXTRINA "SABOR CAPRINO" DO IOGURTE DE LEITE DE CABRA

2001 ◽  
Vol 19 (1) ◽  
Author(s):  
DEISY ALESSANDRA DRUNKLER ◽  
ROSEANE FETT ◽  
MARILDE T. BORDIGNON LUIZ
Keyword(s):  
Milk Protein ◽  
Chemical Properties ◽  
Goat Milk ◽  
Cow Milk ◽  
Physical Chemical ◽  
Total Carbohydrates ◽  
Strawberry Flavor ◽  
Scale Test ◽  
Sensorial Evaluation ◽  
And Control

Avaliou-se a influência da betaciclodextrina (beta-CD) na qualidade sensorial, na composição química e propriedades físicoquímicas (proteína, lipídios, sólidos totais, cinzas, acidez em ácido láctico, carboidratos totais e pH) do iogurte de leite de cabra, sabor morango. Para a avaliação sensorial utilizouse o teste de categoria. Os resultados demonstraram que houve diferença significativa ao nível de 1% de probabilidade entre os tratamentos teste e controle, para o iogurte contendo 0,40% de beta-CD, mostrando a eficiência da beta-CD na minimização do "sabor caprino" nesta concentração. As análises físico-químicas não apresentaram diferença estatística entre os tratamentos. O iogurte de leite de cabra adicionado de beta-CD pode tornar-se mais uma alternativa para o beneficiamento de leite de cabra e mais uma opção para indivíduos alérgicos às proteínas do leite de vaca. Abstract The influence of betaciclodextrin (betacd ) was evaluated in sensorial quality, chemical composition and physical chemical properties (protein, lipids, total solids, ashes, acidity in lactic acid, total carbohydrates and pH) of goat milk yogurt, strawberry flavor. For sensorial evaluation the category scale test was utilized. The results demonstrated significant differences at level of 1% probability between the test and control treatments, for the yogurt containing 0,40% of betaCD, showing the efficiency of betaCD in the minimization of the goaty flavor in this concentration. The physical chemical analysis didn’t show significant statistical difference between the treatments. The goat milk yogurt with addition of betaCD can become another alternative for goat milk processing and other option for allergic individuals to cow milk protein.

scholarly journals Camel Milk Is a Safer Choice than Goat Milk for Feeding Children with Cow Milk Allergy

ISRN Allergy ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Mohammad Ehlayel ◽  
Abdulbari Bener ◽  
Khalid Abu Hazeima ◽  
Fatima Al-Mesaifri
Keyword(s):  
Atopic Dermatitis ◽  
Prospective Study ◽  
Goat Milk ◽  
Specific Ige ◽  
Cross Reactivity ◽  
Medical Examination ◽  
Camel Milk ◽  
Cow Milk ◽  
Milk Allergy ◽  
Cow Milk Allergy

Background. Various sources of mammalian milk have been tried in CMA. Objectives. To determine whether camel milk is safer than goat milk in CMA. Methods. Prospective study conducted at Hamad Medical Corporation between April 2007 and April 2010, on children with CMA. Each child had medical examination, CBC, total IgE, cow milk-specific IgE and SPT. CMA children were tested against fresh camel and goat milks. Results. Of 38 children (median age 21.5 months), 21 (55.3%) presented with urticaria, 17 (39.5%) atopic dermatitis, 10 (26.3%) anaphylaxis. WBC was 10,039±4,735 cells/μL, eosinophil 1,143±2,213 cells/μL, IgE 694±921 IU/mL, cow's milk-specific-IgE 23.5±35.6 KU/L. Only 7 children (18.4%) tested positive to camel milk and 24 (63.2%) to goat milk. 6 (15.8%) were positive to camel, goat, and cow milks. Patients with negative SPT tolerated well camel and goat milks. Conclusions. In CMA, SPT indicates low cross-reactivity between camel milk and cow milk, and camel milk is a safer alternative than goat milk.

scholarly journals DIET THERAPY IN CHILDREN WITH FOOD ALLERGY

2013 ◽  
Vol 10 (5) ◽  
pp. 70-75
Author(s):  
E E Varlamov ◽  
A N Pampura
Keyword(s):  
Atopic Dermatitis ◽  
Food Allergy ◽  
Amino Acid ◽  
Diet Therapy ◽  
Average Value ◽  
Multiple Food Allergy ◽  
Amino Acid Formula

Introduction. Experience of amino acid formulas application in eldest children is limited. Background. To estimate efficiency of amino acid formula at children at the age from 1 till 10 years with atopic dermatitis (AD). Methods. 9 AD children with multiple food allergy were included in the study. All patients took amino acid formula «Neocate Advance». The efficiency of diet therapy was estimated with index SCORAD. Results. The average value of index SCORAD decreased AFTER 1 week therapy, Me — 48,5 [44,5; 52,5] to Me — 18,8 [18; 37,5] a point (p=0,008), after 2 weeks — Me — 12,6 [7,8; 13,5] a point (p=0,008). Conclusion. Medical amino acid formula «Neocate Advance» is an effective diet therapy of AD children with multiple food allergy.

scholarly journals ASSESSMENT OF CLINICAL-IMMUNOLOGICAL EFFECTIVENESS OF COMPLEX THERAPY OF INFANT ATOPIC DERMATITIS

2013 ◽  
Vol 10 (5) ◽  
pp. 55-60
Author(s):  
S N Denisova ◽  
T B Sentsova ◽  
M Y Belitskaya
Keyword(s):  
Atopic Dermatitis ◽  
Milk Protein ◽  
Protein Hydrolysate ◽  
Blood Stream ◽  
Diet Therapy ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Complex Therapy ◽  
Specific Igg ◽  
Cow’S Milk Protein

The work is aimed at assessment of clinicalimmunological effectiveness of diet therapy based on hypoallergenic formula used for infants with atopic dermatitis. 167 infants with atopic dermatitis associated with cow’s milk protein allergy have been put included under outpatient observation. 52 infants of from the first group were being fed on whole goat milk’s formula, 56 infants of from the the second group — cows’ milk protein hydrolysate and 59 infants of the from the third groupsoy’s isolate formula. The age of infants was 2, 5—9 months. The duration of observation was 3—12 months. Complex therapy has been conducted over the infants and it contained antiallergenic treatment and diet therapy with hypoallergenic baby formula. The majority of patients showed improvement due to applied treatment. The first group’s infants with positive clinical dynamic showed decrease of specific IgG and IgE to cow’s milk protein, casein, β-LH and soy’s protein. The infants of the second and third groups also showed decrease of specific IgG and IgE to cow’s milk protein, casein, β-LH, soy’s protein and goat’s milk at clinical remission. All the infants with positive clinical effect showed trend to decrease of IL12, IL13, sICAM1 and sEselectin’s concentration in the blood stream, that can indicate on leukocyte adherence inhibition and cell migration to the area of inflammation. Based on the results of research, we can conclude that replacement of one product with another one by taking into account their clinicalimmunological effectiveness as the method of treatment the of atopic dermatitis patients let us optimize infant diet therapy.

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