Efficiency of Chicken Blastoderm Cell Transfection Combining two Nonviral Methods: Electroporation and Synthetic Carriers

2017 ◽  
Vol 65 (1) ◽  
pp. 32-40
Author(s):  
Anita Kinal ◽  
Izabela Kozłowska ◽  
Paweł Łakota ◽  
Magdalena Wawrzyńska ◽  
Ewelina Kijewska
Keyword(s):  
Cell Transfection ◽  
Blastoderm Cell

scholarly journals The Double Mutation DSG2-p.S363X and TBX20-p.D278X Is Associated with Left Ventricular Non-Compaction Cardiomyopathy: Case Report

2021 ◽  
Vol 22 (13) ◽  
pp. 6775
Author(s):  
Roman Myasnikov ◽  
Andreas Brodehl ◽  
Alexey Meshkov ◽  
Olga Kulikova ◽  
Anna Kiseleva ◽  
...  
Keyword(s):  
Genetic Screening ◽  
Ventricular Dysfunction ◽  
Layer Structure ◽  
Left Ventricular ◽  
Cell Transfection ◽  
Nonsense Mutations ◽  
Double Mutation ◽  
T Box ◽  
Generation Sequencing

Left ventricular non-compaction cardiomyopathy (LVNC) is a rare heart disease, with or without left ventricular dysfunction, which is characterized by a two-layer structure of the myocardium and an increased number of trabeculae. The study of familial forms of LVNC is helpful for risk prediction and genetic counseling of relatives. Here, we present a family consisting of three members with LVNC. Using a next-generation sequencing approach a combination of two (likely) pathogenic nonsense mutations DSG2-p.S363X and TBX20-p.D278X was identified in all three patients. TBX20 encodes the cardiac T-box transcription factor 20. DSG2 encodes desmoglein–2, which is part of the cardiac desmosomes and belongs to the cadherin family. Since the identified nonsense variant (DSG2-p.S363X) is localized in the extracellular domain of DSG2, we performed in vitro cell transfection experiments. These experiments revealed the absence of truncated DSG2 at the plasma membrane, supporting the pathogenic relevance of DSG2-p.S363X. In conclusion, we suggest that in the future, these findings might be helpful for genetic screening and counseling of patients with LVNC.

scholarly journals Capsid-like biodegradable poly-glycolic acid nanoparticles for a long-time release of nucleic acid molecules

2021 ◽  
Author(s):  
Clara Guido ◽  
Mariangela Testini ◽  
Stefania D’Amone ◽  
Barbara Cortese ◽  
Maria Grano ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Glycolic Acid ◽  
Cell Transfection ◽  
Biodegradable Poly ◽  
Long Time ◽  
2D And 3D

Capsid-like PGA nanoparticles (NPs) allow sustained cell transfection in 2D and 3D configurations.

3D nanochannel electroporation for high-throughput cell transfection with high uniformity and dosage control

Nanoscale ◽  
2016 ◽  
Vol 8 (1) ◽  
pp. 243-252 ◽  
Author(s):  
Lingqian Chang ◽  
Paul Bertani ◽  
Daniel Gallego-Perez ◽  
Zhaogang Yang ◽  
Feng Chen ◽  
...  
Keyword(s):  
High Throughput ◽  
Cell Transfection ◽  
High Uniformity

Toward an objective evaluation of cell transfection performance

2010 ◽  
Vol 97 (15) ◽  
pp. 153702
Author(s):  
Viviana Orlando ◽  
Daniela Pozzi ◽  
Giulio Caracciolo ◽  
Gabriella Augusti-Tocco ◽  
Stefano Biagioni
Keyword(s):  
Objective Evaluation ◽  
Cell Transfection

scholarly journals Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yu Ting Chow ◽  
Shuxun Chen ◽  
Ran Wang ◽  
Chichi Liu ◽  
Chi-wing Kong ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Transfection

Research on effective cell transfection platform using the needle-free bubble injector

2020 ◽  
Vol 2020.31 (0) ◽  
pp. 1A16
Author(s):  
Wenjing HUANG ◽  
Shigeo S. SUGANO ◽  
Akiho HIRAO ◽  
Naoto TOTTORI ◽  
Yoko YAMANISHI
Keyword(s):  
Cell Transfection

Angiogenic Polypeptides in Breast Cancer: Expression of Mrna’s in Primary Human Tumours, MCF-7 Cell Transfection and Xenograft Models

Angiogenesis ◽  
1998 ◽  
pp. 213-221
Author(s):  
Hua-Tang Zhang ◽  
Rangana Choudhuri ◽  
Prudence AE Scott ◽  
Lyna Zhang ◽  
Marina Ziche ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Transfection ◽  
Human Tumours ◽  
Xenograft Models ◽  
Mcf 7

scholarly journals Peptide–siRNA Supramolecular Particles for Neural Cell Transfection

2018 ◽  
Vol 6 (3) ◽  
pp. 1801458 ◽  
Author(s):  
Armando Hernandez‐Garcia ◽  
Zaida Álvarez ◽  
Dina Simkin ◽  
Ashwin Madhan ◽  
Eloise Pariset ◽  
...  
Keyword(s):  
Neural Cell ◽  
Cell Transfection

An injectable paste of calcium phosphate nanorods, functionalized with nucleic acids, for cell transfection and gene silencing

2010 ◽  
Vol 20 (29) ◽  
pp. 6144 ◽  
Author(s):  
J. Klesing ◽  
S. Chernousova ◽  
A. Kovtun ◽  
S. Neumann ◽  
L. Ruiz ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Nucleic Acids ◽  
Gene Silencing ◽  
Cell Transfection
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