An injectable paste of calcium phosphate nanorods, functionalized with nucleic acids, for cell transfection and gene silencing

2010 ◽  
Vol 20 (29) ◽  
pp. 6144 ◽  
Author(s):  
J. Klesing ◽  
S. Chernousova ◽  
A. Kovtun ◽  
S. Neumann ◽  
L. Ruiz ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Nucleic Acids ◽  
Gene Silencing ◽  
Cell Transfection

Deciphering the response of asymmetry in the hydrophobic chains of novel cationic lipids towards biological function

2020 ◽  
Vol 22 (3) ◽  
pp. 1738-1746 ◽  
Author(s):  
Dipanjan Mukherjee ◽  
Priya Singh ◽  
Tatini Rakshit ◽  
Theja P. Puthiya-Purayil ◽  
Praveen Kumar Vemula ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Biological Function ◽  
Cationic Liposome ◽  
Cationic Lipids ◽  
Cell Transfection ◽  
Molecular Architecture

Variations in molecular architecture of the hydrophobic tails of cationic lipids influence cationic liposome*s efficiency of delivering nucleic acids during cell transfection.

F127/Calcium phosphate hybrid nanoparticles: a promising vector for improving siRNA delivery and gene silencing

2013 ◽  
Vol 24 (15) ◽  
pp. 1757-1766 ◽  
Author(s):  
Liubin Qin ◽  
Ying Sun ◽  
Peifeng Liu ◽  
Qi Wang ◽  
Baosan Han ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Gene Silencing ◽  
Sirna Delivery ◽  
Hybrid Nanoparticles

Cell Transfection Using Calcium Phosphate Ceramic

2004 ◽  
Vol 3 (4) ◽  
pp. 57-59 ◽  
Author(s):  
Patrick Frayssinet ◽  
André Guilhem
Keyword(s):  
Calcium Phosphate ◽  
Cell Transfection ◽  
Calcium Phosphate Ceramic

Colonic gene silencing using siRNA-loaded calcium phosphate/PLGA nanoparticles ameliorates intestinal inflammation in vivo

2016 ◽  
Vol 222 ◽  
pp. 86-96 ◽  
Author(s):  
Annika Frede ◽  
Bernhard Neuhaus ◽  
Robert Klopfleisch ◽  
Catherine Walker ◽  
Jan Buer ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Gene Silencing ◽  
Intestinal Inflammation ◽  
Plga Nanoparticles

scholarly journals Functionalized Calcium Phosphate Nanoparticles for Biomedical Application

2010 ◽  
Vol 441 ◽  
pp. 299-305 ◽  
Author(s):  
Matthias Epple ◽  
Anna Kovtun
Keyword(s):  
Calcium Phosphate ◽  
Nucleic Acids ◽  
Intracellular Calcium ◽  
Calcium Level ◽  
Biomedical Application ◽  
Hard Tissue ◽  
Chemical Similarity ◽  
Monodisperse Nanoparticles ◽  
Intracellular Calcium Level ◽  
Calcium Phosphate Nanoparticles

Calcium phosphate is a natural biomineral and therefore possesses an excellent biocompatibility due to its chemical similarity to human hard tissue (bone and teeth). Calcium phosphate nanoparticles can be precipitated under controlled conditions and used as carrier in biological systems, e.g. to transfer nucleic acids or drugs. Such nanoparticles can also be suitably functionalized with fluorescing dyes, polymeric agents, pro-drugs or activators. The small monodisperse nanoparticles only mildly influence the intracellular calcium level and therefore are not toxic for cells.

Functionalisation of calcium phosphate nanoparticles by oligonucleotides and their application for gene silencing

2007 ◽  
Vol 17 (8) ◽  
pp. 721-727 ◽  
Author(s):  
Viktoriya Sokolova ◽  
Anna Kovtun ◽  
Oleg Prymak ◽  
Wolfgang Meyer-Zaika ◽  
Elena A. Kubareva ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Gene Silencing ◽  
Calcium Phosphate Nanoparticles

Synthetic α-l-Threose Nucleic Acids Targeting BcL-2 Show Gene Silencing and in Vivo Antitumor Activity for Cancer Therapy

2019 ◽  
Vol 11 (42) ◽  
pp. 38510-38518 ◽  
Author(s):  
Fei Wang ◽  
Ling Sum Liu ◽  
Cia Hin Lau ◽  
Tristan Juin Han Chang ◽  
Dick Yan Tam ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Antitumor Activity ◽  
Nucleic Acids ◽  
Gene Silencing ◽  
In Vivo Antitumor Activity

Calcium phosphate nanoparticles prepared from infusion fluids for stem cell transfection: process optimization and cytotoxicity analysis

2017 ◽  
Vol 5 (5) ◽  
pp. 972-981 ◽  
Author(s):  
Quazi T. H. Shubhra ◽  
Ayako Oyane ◽  
Hiroko Araki ◽  
Maki Nakamura ◽  
Hideo Tsurushima
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Process Optimization ◽  
Cell Transfection ◽  
Calcium Phosphate Nanoparticles ◽  
Infusion Fluids

The preparation of calcium phosphate nanoparticles from infusion fluids for gene delivery to stem cells and CHO-K1 cells is reported.

scholarly journals Resveratrol Enhances mRNA and siRNA Lipid Nanoparticles Primary CLL Cell Transfection

Pharmaceutics ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 520
Author(s):  
Edo Kon ◽  
Inbal Hazan-Halevy ◽  
Daniel Rosenblum ◽  
Niv Cohen ◽  
Sushmita Chatterjee ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Nucleic Acids ◽  
Caffeic Acid ◽  
Targeted Therapies ◽  
Lipid Nanoparticles ◽  
Lymphocytic Leukemia ◽  
Cell Transfection ◽  
Formidable Challenge ◽  
Patient Heterogeneity ◽  
Adult Leukemia

Chronic lymphocytic leukemia (CLL) is the most common adult leukemia in Western populations. Therapies such as mRNA and siRNA encapsulated in lipid nanoparticles (LNPs) represent a clinically advanced platform and are utilized for a wide variety of applications. Unfortunately, transfection of RNA into CLL cells remains a formidable challenge and a bottleneck for developing targeted therapies for this disease. Therefore, we aimed to elucidate the barriers to efficient transfection of RNA-encapsulated LNPs into primary CLL cells to advance therapies in the future. To this end, we transfected primary CLL patient samples with mRNA and siRNA payloads encapsulated in an FDA-approved LNP formulation and characterized the transfection. Additionally, we tested the potential of repurposing caffeic acid, curcumin and resveratrol to enhance the transfection of nucleic acids into CLL cells. The results demonstrate that the rapid uptake of LNPs is required for successful transfection. Furthermore, we demonstrate that resveratrol enhances the delivery of both mRNA and siRNA encapsulated in LNPs into primary CLL patient samples, overcoming inter-patient heterogeneity. This study points out the important challenges to consider for efficient RNA therapeutics for CLL patients and advocates the use of resveratrol in combination with RNA lipid nanoparticles to enhance delivery into CLL cells.

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