scholarly journals Copper (II) Ion-Modified Gold Nanoclusters as Peroxidase Mimetics for the Colorimetric Detection of Pyrophosphate

Sensors ◽  
2021 ◽  
Vol 21 (16) ◽  
pp. 5538
Author(s):  
Yunjing Shi ◽  
Jinjie Wang ◽  
Kun Mu ◽  
Suqin Liu ◽  
Guang Yang ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Detection Limit ◽  
Chemical Stability ◽  
Human Urine ◽  
Detection Method ◽  
Colorimetric Detection ◽  
Gold Nanoclusters ◽  
High Sensitivity ◽  
Sensitivity And Selectivity ◽  
Optimized Conditions

Copper (II) ions have been shown to greatly improve the chemical stability and peroxidase-like activity of gold nanoclusters (AuNCs). Since the affinity between Cu2+ and pyrophosphate (PPi) is higher than that between Cu2+ and AuNCs, the catalytic activity of AuNCs-Cu2+ decreases with the introduction of PPi. Based on this principle, a new colorimetric detection method of PPi with high sensitivity and selectivity was developed by using AuNCs-Cu2+ as a probe. Under optimized conditions, the detection limit of PPi was 0.49 nM with a linear range of 0.51 to 30,000 nM. The sensitivity of the method was three orders of magnitude higher than that of a fluorescence method using AuNCs-Cu2+ as the probe. Finally, the AuNCs-Cu2+ system was successfully applied to directly determine the concentration of PPi in human urine samples.

scholarly journals A highly selective and sensitive fluorescent probe for lactate dehydrogenase based on ultrabright adenosine monophosphate capped gold nanoclusters

RSC Advances ◽  
2017 ◽  
Vol 7 (22) ◽  
pp. 13438-13443 ◽  
Author(s):  
Jiao Liu ◽  
Hong-Wei Li ◽  
Yuqing Wu
Keyword(s):  
Lactate Dehydrogenase ◽  
Detection Limit ◽  
Fluorescent Probe ◽  
Fluorescence Probe ◽  
Gold Nanoclusters ◽  
High Sensitivity ◽  
Adenosine Monophosphate ◽  
Low Detection Limit ◽  
Sensitivity And Selectivity

The ultrabright AuNCs@AMP are used as fluorescence probe to detect lactate dehydrogenase (LDH) with high sensitivity and selectivity, showing an extremely low detection limit of 0.2 nM (26 pg μL−1, 0.8 U L−1).

Stable gold nanoparticles as a novel peroxidase mimic for colorimetric detection of cysteine

2016 ◽  
Vol 8 (11) ◽  
pp. 2494-2501 ◽  
Author(s):  
Rong Sheng Li ◽  
Hai Liu ◽  
Bin Bin Chen ◽  
Hong Zhi Zhang ◽  
Cheng Zhi Huang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Peroxidase Mimic ◽  
Sensitivity And Selectivity ◽  
Blue Solution

In this work, stable gold nanoparticles (AuNPs) were prepared with good compatibility and favorable catalytic activity. They were developed as a peroxidase mimic for colorimetric detection of cysteine with a weakened blue solution, which supplied high sensitivity and selectivity.

One-pot synthesized DNA-templated Ag/Pt bimetallic nanoclusters as peroxidase mimics for colorimetric detection of thrombin

2014 ◽  
Vol 50 (86) ◽  
pp. 13103-13106 ◽  
Author(s):  
Cheng Zheng ◽  
Ai-Xian Zheng ◽  
Bo Liu ◽  
Xiao-Long Zhang ◽  
Yu He ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Colorimetric Detection ◽  
Protein Detection ◽  
High Sensitivity ◽  
One Pot ◽  
Highly Efficient ◽  
Bimetallic Nanoclusters ◽  
Peroxidase Mimics ◽  
Sensitivity And Selectivity ◽  
Colorimetric Aptasensor

One-pot synthesized DNA-templated Ag/Pt bimetallic nanoclusters possess highly-efficient peroxidase-like catalytic activity and are applied to design a colorimetric aptasensor for the protein detection with high sensitivity and selectivity.

Exploring a new rapid colorimetric detection method of Cu2+ with high sensitivity and selectivity

2013 ◽  
Vol 176 ◽  
pp. 906-912 ◽  
Author(s):  
Li-Jing Miao ◽  
Jun-Wei Xin ◽  
Zhe-Yu Shen ◽  
Yu-Jie Zhang ◽  
Hai-Yan Wang ◽  
...  
Keyword(s):  
Detection Method ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Sensitivity And Selectivity

scholarly journals Effective reduction of nitrophenols and colorimetric detection of Pb(ii) ions by Siraitia grosvenorii fruit extract capped gold nanoparticles

RSC Advances ◽  
2021 ◽  
Vol 11 (25) ◽  
pp. 15438-15448
Author(s):  
Van Thuan Le ◽  
Truong Giang Duong ◽  
Van Tan Le ◽  
Thanh Long Phan ◽  
Thi Lan Huong Nguyen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Fruit Extract ◽  
Siraitia Grosvenorii ◽  
Effective Reduction ◽  
Sensitivity And Selectivity

Siraitia grosvenorii fruit extract capped AuNPs exhibited excellent catalytic activity in the reduction of nitrophenols and high sensitivity and selectivity for detection of Pb(ii) ions.

scholarly journals Fluorescence Sensing Platforms for Epinephrine Detection Based on Low Temperature Cofired Ceramics

Sensors ◽  
2020 ◽  
Vol 20 (5) ◽  
pp. 1429
Author(s):  
Sylwia Baluta ◽  
Karol Malecha ◽  
Agnieszka Świst ◽  
Joanna Cabaj
Keyword(s):  
Detection Limit ◽  
Low Temperature ◽  
High Sensitivity ◽  
Fluorescence Sensing ◽  
Free System ◽  
Injection Test ◽  
Sensing Platforms ◽  
Sensitivity And Selectivity ◽  
Cofired Ceramics ◽  
Optimized Conditions

A novel fluorescence-sensing pathway for epinephrine (EP) detection was investigated. The ceramic-based miniature biosensor was developed through the immobilization of an enzyme (laccase, tyrosinase) on a polymer—poly-(2,6-di([2,2′-bithiophen]-5-yl)-4-(5-hexylthiophen-2-yl)pyridine), based on low temperature cofired ceramics technology (LTCC). The detection procedure was based on the oxidation of the substrate, i.e., in the presence of the enzyme. An alternative enzyme-free system utilized the formation of a colorful complex between Fe2+ ions and epinephrine molecules. With the optimized conditions, the analytical performance illustrated high sensitivity and selectivity in a broad linear range with a detection limit of 0.14–2.10 nM. Moreover, the strategy was successfully used for an EP injection test with labeled pharmacological samples.

scholarly journals Enhancement of the Peroxidase-Like Activity of Iodine-Capped Gold Nanoparticles for the Colorimetric Detection of Biothiols

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 113 ◽  
Author(s):  
Chia-Chen Chang ◽  
Tsz-Lian Hsu ◽  
Chie-Pein Chen ◽  
Chen-Yu Chen
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Detection Limit ◽  
Enzymatic Activity ◽  
Catalytic Properties ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Synergetic Effect ◽  
Vis Spectroscopy ◽  
Wide Range

A colorimetric assay was developed for the detection of biothiols, based on the peroxidase-like activity of iodine-capped gold nanoparticles (AuNPs). These AuNPs show a synergetic effect in the form of peroxidase-mimicking activity at the interface of AuNPs, while free AuNPs and iodine alone have weak catalytic properties. Thus, iodine-capped AuNPs possess good intrinsic enzymatic activity and trigger the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), leading to a change in color from colorless to yellow. When added to solution, biothiols, such as cysteine, strongly bind to the interface of AuNPs via gold-thiol bonds, inhibiting the catalytic activity of AuNPs, resulting in a decrease in oxidized TMB. Using this strategy, cysteine could be linearly determined, at a wide range of concentrations (0.5 to 20 μM), with a detection limit of 0.5 μM using UV-Vis spectroscopy. This method was applied for the detection of cysteine in diluted human urine.

scholarly journals Colorimetric Detection of Sulfide Anions via Redox-Modulated Surface Chemistry and Morphology of Au-Hg Nanorods

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaotian Zhu ◽  
Chang Liu ◽  
Jie Liu
Keyword(s):  
Surface Chemistry ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Localized Surface Plasmon ◽  
Wavelength Shift ◽  
Colorimetric Sensing ◽  
Leather Industry ◽  
Colorimetric Response ◽  
Sensitivity And Selectivity

A new colorimetric assay for the detection of sulfide anions with high sensitivity and selectivity is reported, utilizing Au-Hg alloy nanorods (Au-HgNRs) as probe. Au-HgNRs were prepared by modifying gold nanorods (AuNRs) with reducing agent and mercury ions. In an aqueous solution with sulfide anions, the formation of mercuric sulfide due to redox reaction between the amalgams and sulfide anions greatly changed the surface chemistry and morphology of the Au-HgNRs, leading to a red shift of the localized surface plasmon resonance (LSPR) absorption peak, accompanied by a change in colorimetric response. A good linear relationship was obtained between the LSPR peak wavelength shift and concentration of sulfide anion in the range of 1 × 10−5−1 × 10−4 mol/L. The selectivity of this method has been investigated by other anions. The colorimetric sensing system successfully detected sulfide in wastewater from leather industry.

scholarly journals Label-Free Split Aptamer Sensor for Femtomolar Detection of Dopamine by Means of Flexible Organic Electrochemical Transistors

Materials ◽  
2020 ◽  
Vol 13 (11) ◽  
pp. 2577 ◽  
Author(s):  
Yuanying Liang ◽  
Ting Guo ◽  
Lei Zhou ◽  
Andreas Offenhäusser ◽  
Dirk Mayer
Keyword(s):  
Detection Limit ◽  
Electrochemical Sensors ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Label Free ◽  
Dopamine Detection ◽  
Electrochemical Transistor ◽  
Sensitivity And Selectivity

The detection of chemical messenger molecules, such as neurotransmitters in nervous systems, demands high sensitivity to measure small variations, selectivity to eliminate interferences from analogues, and compliant devices to be minimally invasive to soft tissue. Here, an organic electrochemical transistor (OECT) embedded in a flexible polyimide substrate is utilized as transducer to realize a highly sensitive dopamine aptasensor. A split aptamer is tethered to a gold gate electrode and the analyte binding can be detected optionally either via an amperometric or a potentiometric transducer principle. The amperometric sensor can detect dopamine with a limit of detection of 1 μM, while the novel flexible OECT-based biosensor exhibits an ultralow detection limit down to the concentration of 0.5 fM, which is lower than all previously reported electrochemical sensors for dopamine detection. The low detection limit can be attributed to the intrinsic amplification properties of OECTs. Furthermore, a significant response to dopamine inputs among interfering analogues hallmarks the selective detection capabilities of this sensor. The high sensitivity and selectivity, as well as the flexible properties of the OECT-based aptasensor, are promising features for their integration in neuronal probes for the in vitro or in vivo detection of neurochemical signals.

Study of Glucose Binding Protein Encapsulated Gold Nanoclusters by Molecular Dynamic Simulation

2019 ◽  
Vol 948 ◽  
pp. 133-139
Author(s):  
Boy Marsaputra Panjaitan ◽  
Karina Kubiak-Ossowska ◽  
David Birch ◽  
Yu Chen
Keyword(s):  
Md Simulation ◽  
Glucose Sensor ◽  
Binding Protein ◽  
Gold Nanoclusters ◽  
High Sensitivity ◽  
Nucleation Site ◽  
Fluorescent Properties ◽  
Glucose Binding ◽  
Sensitivity And Selectivity ◽  
Glucose Binding Protein

Protein encapsulated gold nanoclusters has attracted great attention for their excellent fluorescent properties and potential biomedical applications. Glucose Binding Protein (GBP) has a high sensitivity and selectivity to glucose binding that makes them ideal for biosensor development. It is anticipated that GBP encapsulated gold nanoclusters could be a promising glucose sensor. Here we investigated the growth of gold nanoclusters in GBP using Molecular Dynamics (MD) simulation. To facilitation the nucleation of gold nanoclusters at specific sites, cysteine mutations were introduced in GBP. It is found that the nucleation site of gold nanoclusters inside mutant GBP are different from those in native GBP. Gold nanoclusters were formed near the mutated cysteine and tyrosine residues. Glucose remained in the binding site of a mutant GBP with gold nanoclusters although no conformational change was observed in MD simulation, similar to a native GBP. This work suggests the possibility of growing gold nanoclusters in the designed site within GBP and a new glucose sensor based on mutated GBP protected gold nanoclusters.

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