scholarly journals Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1235 ◽  
Author(s):  
Iva Pernicova ◽  
Ivana Novackova ◽  
Petr Sedlacek ◽  
Xenie Kourilova ◽  
Michal Kalina ◽  
...  
Keyword(s):  
Dry Mass ◽  
Production Potential ◽  
Biotechnological Production ◽  
Promising Candidate ◽  
Isolation And Characterization ◽  
Extremophilic Microorganisms ◽  
Positive Isolate ◽  
Pha Copolymers ◽  
Very High

Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolate Aneurinibacillus sp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 °C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated that Aneurinibacillus sp. H1 is a very promising candidate for production of PHA with tailored material properties.

scholarly journals Isolation and Characterization of Strain Exiguobacterium sp. KRL4, a Producer of Bioactive Secondary Metabolites from a Tibetan Glacier

2021 ◽  
Vol 9 (5) ◽  
pp. 890
Author(s):  
Pietro Tedesco ◽  
Fortunato Palma Esposito ◽  
Antonio Masino ◽  
Giovanni Andrea Vitale ◽  
Emiliana Tortorella ◽  
...  
Keyword(s):  
Phenotypic Analysis ◽  
Biological Assays ◽  
C Elegans ◽  
Isolation And Characterization ◽  
Wide Range ◽  
Protein Encoding ◽  
Nematocidal Activity ◽  
Extremophilic Microorganisms ◽  
Unique Source

Extremophilic microorganisms represent a unique source of novel natural products. Among them, cold adapted bacteria and particularly alpine microorganisms are still underexplored. Here, we describe the isolation and characterization of a novel Gram-positive, aerobic rod-shaped alpine bacterium (KRL4), isolated from sediments from the Karuola glacier in Tibet, China. Complete phenotypic analysis was performed revealing the great adaptability of the strain to a wide range of temperatures (5–40 °C), pHs (5.5–8.5), and salinities (0–15% w/v NaCl). Genome sequencing identified KRL4 as a member of the placeholder genus Exiguobacterium_A and annotation revealed that only half of the protein-encoding genes (1522 of 3079) could be assigned a putative function. An analysis of the secondary metabolite clusters revealed the presence of two uncharacterized phytoene synthase containing pathways and a novel siderophore pathway. Biological assays confirmed that the strain produces molecules with antioxidant and siderophore activities. Furthermore, intracellular extracts showed nematocidal activity towards C. elegans, suggesting that strain KRL4 is a source of anthelmintic compounds.

Studies of the Composition and Structure of Serum Lipoproteins. Isolation and Characterization of Very High Density Lipoproteins of Human Serum*

Biochemistry ◽  
1966 ◽  
Vol 5 (12) ◽  
pp. 4044-4053 ◽  
Author(s):  
Petar Alaupovic ◽  
Shafeek S. Sanbar ◽  
Robert H. Furman ◽  
Michael L. Sullivan ◽  
Sandra L. Walraven
Keyword(s):  
Human Serum ◽  
High Density ◽  
High Density Lipoproteins ◽  
Serum Lipoproteins ◽  
Isolation And Characterization ◽  
Composition And Structure ◽  
Very High

ISOLATION AND CHARACTERIZATION OF DIFFERENT TYPES OF PANCREATIC ISLET CELLS IN GUINEA-PIGS

1966 ◽  
Vol 53 (3) ◽  
pp. 480-488 ◽  
Author(s):  
Birger Petersson
Keyword(s):  
B Cells ◽  
Islet Cells ◽  
Dry Mass ◽  
Endocrine Activity ◽  
Isolation And Characterization ◽  
Different Types ◽  
Gentle Pressure ◽  
Staining Methods ◽  
Isolated Islet Cells

ABSTRACT By means of gentle pressure on isolated guinea-pig islets, cells in an isolated state were obtained. The free islet cells could be classified as A1, A2 and B cells with the current staining methods. Determinations of the dry mass with a scanning interference microphotometer showed a significantly higher value in the A2 cells (1.95 × 10−10 g) as compared with the B cells (1.12 × 10−10 g). Various possibilities for utilizing the isolated islet cells for further analyses of the endocrine activity of the pancreas are discussed.

scholarly journals Isolation and Characterization of a Putative Ascorbate Peroxidase from Citrus Center

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 862B-862
Author(s):  
MadhuraBabu Kunta ◽  
Eliezer Louzada*
Keyword(s):  
Ascorbate Peroxidase ◽  
Amino Acid Level ◽  
Rt Pcr ◽  
Conserved Motifs ◽  
E Coli ◽  
Isolation And Characterization ◽  
Active Site Motif ◽  
Glutathione Pathway ◽  
Very High

Ascorbate Peroxidase (APX) is a heme-containing, non-glycosylated enzyme that destroys harmful hydrogen peroxide via the ascorbate glutathione pathway in plants. This enzyme is considered to be an indispensable part of the electron-scavenging pathway and is involved in preventing oxidative damage in plants. Using differential display RT-PCR and 5' RACE a full length c-DNA clone was isolated, from citrus, with very high similarity at the nucleotide and amino acid level, to ascorbate peroxidases from several plant species. It is well known that APXs have highly conserved motifs like the Arg-38, Ars-71, Glu-65 and Asp-208 residues around the distal Hist-42 and proximal His-163. These residues are essential for binding the ligand heme. Additionally, Trp-179 is conserved in most APXs and is the third participant in hydrogen bonding network, together with His-163 and Asp-208. All these conserved motifs were present in the putative APX from citrus in addition of the presence of the peroxidase active site motif residues (APITLRLAWHSA) and the peroxidase heme-ligand motif (DIVVLSGGHTL). Expression analysis in E. Coli reviewed a recombinant protein of 27 Kda.

scholarly journals Preliminary Studies on Fungal Contamination of Two Rupestrian Churches from Matera (Southern Italy)

2020 ◽  
Vol 12 (17) ◽  
pp. 6988
Author(s):  
Stefania Mirela Mang ◽  
Laura Scrano ◽  
Ippolito Camele
Keyword(s):  
Fungal Species ◽  
World Heritage Site ◽  
Detailed Knowledge ◽  
Unesco World Heritage ◽  
Isolation And Characterization ◽  
Heritage Site ◽  
Unesco World Heritage Site ◽  
Lecanicillium Psalliotae ◽  
Very High

The Sassi, a UNESCO World Heritage Site and its rupestrian churches, are richly decorated and visited by thousands of visitors every year. It is important to preserve this heritage which shows signs of deterioration due to abiotic and/or biotic factors. Aiming to carry out in the future an environmental-friendly restoration, a screening of the fungi present on walls and frescoes of two rupestrian churches “Santa Lucia alle Malve” and “La Madonna dei derelitti” located, respectively, in the “Sasso Caveoso” and in the “Sasso Barisano” was performed. Isolation and characterization of fungal species from investigated sites was carried out. Total genomic DNA (gDNA) was extracted from pure fungal cultures and subsequently utilized in PCRs using primers that amplify a portion of the ribosomal DNA (ITS5/ITS4) or the β-tubulin gene (Bt2a/Bt2b). The amplicons were directly sequenced. Obtained nucleotide sequences were compared to those present in the GenBank (NCBI) showing a very high similarity (99–100%) with the following species: Parengyodontium album, Alternaria alternata, Cladosporium cladosporioides, Lecanicillium psalliotae, Meyerozyma guilliermondii and Botryotrichum atrogriseum. All sequences from this study were deposited in the EMBL database. Detailed knowledge about fungi isolated from stone is indispensable not only to counter/reduce the structural and aesthetic damage but also to protect the health of both guardians and visitors who may develop different pathologies due to the spores diffused in the environment.

Isolation and characterization of a bacterium able to degrade high concentrations of iprodione

2018 ◽  
Vol 64 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Li Cao ◽  
Wenhong Shi ◽  
Rundong Shu ◽  
Jian Pang ◽  
Yuetao Liu ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Sole Source ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Promising Candidate ◽  
Physiological And Biochemical Characteristics ◽  
Isolation And Characterization ◽  
High Concentrations ◽  
Physiological And Biochemical

A bacterial strain CQH-1 capable of mineralizing iprodione was isolated and characterized. On the basis of its morphological, physiological, and biochemical characteristics combined with phylogenetic analysis of its 16S rRNA gene sequence, strain CQH-1 was identified as a Microbacterium sp. CQH-1. It was able to use iprodione and 3,5-dichloroaniline as the sole source of carbon and energy for its growth. It completely degraded 100 mg·L−1iprodione within 96 h at 30 °C. During the degradation of iprodione by strain CQH-1, 2 compounds were detected in GC–MS analysis and were recognized as N-(3,5-dichlorophenyl)-2,4-dioxoimidazolidine and 3,5-dichloroaniline. So, the biodegradation pathway of iprodione by strain CQH-1 was proposed. This is the first report of an iprodione-mineralizing strain from the genus Microbacterium, and strain CQH-1 might be a promising candidate for application in the bioremediation of iprodione-contaminated environments.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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