ISOLATION AND CHARACTERIZATION OF DIFFERENT TYPES OF PANCREATIC ISLET CELLS IN GUINEA-PIGS

1966 ◽  
Vol 53 (3) ◽  
pp. 480-488 ◽  
Author(s):  
Birger Petersson
Keyword(s):  
B Cells ◽  
Islet Cells ◽  
Dry Mass ◽  
Endocrine Activity ◽  
Isolation And Characterization ◽  
Different Types ◽  
Gentle Pressure ◽  
Staining Methods ◽  
Isolated Islet Cells

ABSTRACT By means of gentle pressure on isolated guinea-pig islets, cells in an isolated state were obtained. The free islet cells could be classified as A1, A2 and B cells with the current staining methods. Determinations of the dry mass with a scanning interference microphotometer showed a significantly higher value in the A2 cells (1.95 × 10−10 g) as compared with the B cells (1.12 × 10−10 g). Various possibilities for utilizing the isolated islet cells for further analyses of the endocrine activity of the pancreas are discussed.

scholarly journals Isolation and Characterization of Single Anti-U1A-specific B Cells from Autoimmune Patients

1997 ◽  
Vol 815 (1 B-Lymphocytes) ◽  
pp. 440-442 ◽  
Author(s):  
RUUD M. T. DE WILDT ◽  
FRANK H. J. VAN DEN HOOGEN ◽  
WALTHER J. VENROOIJ ◽  
RENÉ M. A. HOET
Keyword(s):  
B Cells ◽  
Isolation And Characterization

Isolation and characterization of plasmid from the Bacillus brevis var. G.-B. cells

1978 ◽  
Vol 164 (2) ◽  
pp. 195-204 ◽  
Author(s):  
A. P. Dobritsa ◽  
Svetlana V. Dobritsa ◽  
V. I. Tanyashin
Keyword(s):  
B Cells ◽  
Bacillus Brevis ◽  
Isolation And Characterization

Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48)

1991 ◽  
Vol 11 (3) ◽  
pp. 1614-1623
Author(s):  
R C Fisher ◽  
D A Thorley-Lawson
Keyword(s):  
B Cells ◽  
Epstein Barr Virus ◽  
Leukocyte Adhesion ◽  
Human Leukocyte ◽  
Lymphoid Cells ◽  
Immunoglobulin Superfamily ◽  
Barr Virus ◽  
Isolation And Characterization ◽  
Epstein Barr

BLAST-1 (CD48) (previously referred to as BCM-1 by the Human Gene Nomenclature Committee) is an early-activation-associated membrane glycoprotein expressed on the surface of human leukocytes and induced to a high level following infection of B cells by the Epstein-Barr virus. It is a member of the immunoglobulin superfamily, mediates cell adhesion, and has significant sequence homology to two other adhesion molecules, CD2 and LFA3. Here we report the isolation and characterization of the BLAST-1 gene. The gene is at least 28.6 kb in length, is split into 4 exons, and contains a restriction fragment-length polymorphism. The overall genomic organization is consistent with other members of the immunoglobulin superfamily, in which extracellular immunoglobulinlike domains are encoded by discrete exons. Transcription is initiated at a series of major and minor sites in both normal and tumor-derived lymphoid cells. Appropriately located TATA and CCAAT box sequences were not detected. These characteristics have also been demonstrated for the recently described B-cell-specific genes B29 and CD20. The expression of these genes in B cells may involve the use of multiple promoters and novel transcription initiator-binding proteins. A 1.58-kb genomic DNA fragment, consisting of the 5'-flanking region located immediately upstream of the ATG initiation codon, was able to drive the expression of a reporter gene in an orientation-dependent and tissue-restricted manner.

scholarly journals Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48).

1991 ◽  
Vol 11 (3) ◽  
pp. 1614-1623 ◽  
Author(s):  
R C Fisher ◽  
D A Thorley-Lawson
Keyword(s):  
B Cells ◽  
Epstein Barr Virus ◽  
Leukocyte Adhesion ◽  
Human Leukocyte ◽  
Lymphoid Cells ◽  
Immunoglobulin Superfamily ◽  
Barr Virus ◽  
Isolation And Characterization ◽  
Epstein Barr

BLAST-1 (CD48) (previously referred to as BCM-1 by the Human Gene Nomenclature Committee) is an early-activation-associated membrane glycoprotein expressed on the surface of human leukocytes and induced to a high level following infection of B cells by the Epstein-Barr virus. It is a member of the immunoglobulin superfamily, mediates cell adhesion, and has significant sequence homology to two other adhesion molecules, CD2 and LFA3. Here we report the isolation and characterization of the BLAST-1 gene. The gene is at least 28.6 kb in length, is split into 4 exons, and contains a restriction fragment-length polymorphism. The overall genomic organization is consistent with other members of the immunoglobulin superfamily, in which extracellular immunoglobulinlike domains are encoded by discrete exons. Transcription is initiated at a series of major and minor sites in both normal and tumor-derived lymphoid cells. Appropriately located TATA and CCAAT box sequences were not detected. These characteristics have also been demonstrated for the recently described B-cell-specific genes B29 and CD20. The expression of these genes in B cells may involve the use of multiple promoters and novel transcription initiator-binding proteins. A 1.58-kb genomic DNA fragment, consisting of the 5'-flanking region located immediately upstream of the ATG initiation codon, was able to drive the expression of a reporter gene in an orientation-dependent and tissue-restricted manner.

scholarly journals Characterization of mouse spermatogonia by transmission electron microscopy

Reproduction ◽  
2002 ◽  
pp. 567-577 ◽  
Author(s):  
H Chiarini-Garcia ◽  
LD Russell
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
B Cells ◽  
Nuclear Envelope ◽  
Type B ◽  
Intermediate Type ◽  
Functional Studies ◽  
Transmission Electron ◽  
Different Types

Characteristics of the various type A, intermediate (In) and B spermatogonia were determined in C57BL/6J mice using transmission electron microscopy. Spermatogonia were photographed at all stages of the cycle of the seminiferous epithelium. Over 450 images were taken. Spermatogonia could be classified into As, Apr, Aal, A1 cells, A2 cells, A3 cells, A4 cells, intermediate type and type B cells primarily on the basis of nuclear and nucleolar characteristics. The most primitive spermatogonia (As, Apr, Aal) had mottled chromatin; A1 cells contained homogeneously finely granular chromatin throughout the nucleus; A2, A3, A4 and intermediate type spermatogonia had progressively increasing amounts of chromatin encrusting the nuclear envelope; type B spermatogonia had less heterochromatin along the nuclear envelope, although the particles were more dense and rounded than in intermediate type spermatogonia. Mitochondrial size and position of Golgi complexes varied in different types of spermatogonia. These data show that types of spermatogonia can be differentiated such that these characteristics can be used in functional studies.

scholarly journals Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1235 ◽  
Author(s):  
Iva Pernicova ◽  
Ivana Novackova ◽  
Petr Sedlacek ◽  
Xenie Kourilova ◽  
Michal Kalina ◽  
...  
Keyword(s):  
Dry Mass ◽  
Production Potential ◽  
Biotechnological Production ◽  
Promising Candidate ◽  
Isolation And Characterization ◽  
Extremophilic Microorganisms ◽  
Positive Isolate ◽  
Pha Copolymers ◽  
Very High

Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolate Aneurinibacillus sp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 °C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated that Aneurinibacillus sp. H1 is a very promising candidate for production of PHA with tailored material properties.

scholarly journals A perspective on the isolation and characterization of extracellular vesicles from different biofluids

RSC Advances ◽  
2021 ◽  
Vol 11 (32) ◽  
pp. 19598-19615
Author(s):  
Reshma Bano ◽  
Farhan Ahmad ◽  
Mohd Mohsin
Keyword(s):  
Extracellular Vesicles ◽  
Detection Methods ◽  
Apoptotic Bodies ◽  
Isolation And Characterization ◽  
Different Types

Isolation and detection methods for the different types of EVs (e.g., exosomes, microvesicles, apoptotic bodies, oncosomes) from biofluids.

scholarly journals Isolation and Characterization of Enterococcus faecalis-Infecting Bacteriophages From Different Cheese Types

2021 ◽  
Vol 11 ◽  
Author(s):  
Beatriz del Rio ◽  
Esther Sánchez-Llana ◽  
Noelia Martínez ◽  
María Fernández ◽  
Victor Ladero ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Fermented Foods ◽  
Isolation And Characterization ◽  
Different Types ◽  
Clinical Environments ◽  
Genomic Characterization ◽  
Diverse Origin ◽  
Hospital Acquired ◽  
Silver Bullet

Enterococci are a diverse group of Gram-positive, lactic acid bacteria (LAB). They are found in many environments, including fermented foods, in which they could constitute a health threat since they produce biogenic amines, which consumption can lead to intoxication. Moreover, enterococci has also emerged as an important hospital-acquired pathogens via its acquisition of antimicrobial resistance. Bacteriophages possess features that make them optimal biotechnological weapons for controlling bacterial growth in disease and food spoilage contexts. However, no silver bullet bacteriophage exists that can eliminate all the undesirable bacteria in a complex environment. Rather, a combination of phages with different host ranges would be required which implies the need for large collections of diverse phages. This work reports the isolation of several Enterococcus faecalis-infecting bacteriophages from different types of cheese, along with the range of E. faecalis strains of diverse origin (from dairy to clinical environments) they are able to infect. The isolated phages showed a large diversity regarding the number and origin of strains they infect. Some of these phages were subjected to morphological and genomic characterization which confirmed their diversity and showed they belong to different families and genera. The present findings increase the potential arsenal for the bacteriophage-based biocontrol of harmful E. faecalis populations.

Isolation and characterization of human B lymphocyte enriched populations. I. Purification of B cells by immune rosette depletion

1983 ◽  
Vol 61 (3) ◽  
pp. 283-292 ◽  
Author(s):  
Kenneth C. Anderson ◽  
James D. Griffin ◽  
Michael P. Bates ◽  
Bruce L. Slaughenhoupt ◽  
Stuart F. Schlossman ◽  
...  
Keyword(s):  
B Cells ◽  
B Lymphocyte ◽  
Isolation And Characterization

scholarly journals ISOLATION AND CHARACTERIZATION OF THE SARCOPLASMIC RETICULUM OF SKELETAL MUSCLE

1972 ◽  
Vol 55 (2) ◽  
pp. 471-488 ◽  
Author(s):  
Jeanine-Anne Heuson-Stiennon ◽  
Jean-Claude Wanson ◽  
Pierre Drochmans
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Calcium Binding ◽  
Plasma Membranes ◽  
Binding Activity ◽  
Positive Staining ◽  
Isolation And Characterization ◽  
Different Types ◽  
Thin Walled

The sarcoplasmic reticulum (SR) of rabbit skeletal muscle was studied after isolation of a vesicle fraction and of vesicular subfractions by means of differential and density gradient centrifugations. The different fractions were examined electron microscopically by negative and positive staining; their content in protein and phospholipid and their ability to bind Ca++ were determined. After homogenization, differential centrifugation yielded a "sarcovesicular fraction" (SVF) which was mainly composed of numerous vesicles of different types mixed with fibrous proteins and mitochondrial fragments. This SVF contained 2% of the protein and 25% of the phospholipid of the initial tissue extract. It had a high Ca++ binding activity that was preserved for several days by storage in the presence of oxalate. After centrifugations of the SVF on sucrose density gradients, two vesicular subfractions were obtained which were characterized by different sedimentation rates, isopycnic banding, morphology, and composition in protein and phospholipid. (a) The low-density subfraction (ρ 1.10–1.12) contained a heterogeneous population of membranous structures: thick- and thin-walled vesicles, tubular formations, triads, and plasma membranes. Its content in protein and phospholipid was very low. (b) The high-density subfraction (ρ 1.13–1.17) was a very pure subfraction composed only of thin-walled vesicles. Its content in phospholipid was high and the ratio of phospholipid-phosphorus to protein was about 20. The calcium-binding activity found in the total SVF was recovered only in this latter homogeneous subfraction. The origin of these two subfractions from the SR is discussed.

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