scholarly journals Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane

Polymers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 622 ◽  
Author(s):  
Meng Qi ◽  
Kongyin Zhao ◽  
Qiwen Bao ◽  
Peng Pan ◽  
Yuwei Zhao ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Adsorption Capacity ◽  
Bovine Serum ◽  
Calcium Alginate ◽  
Selective Adsorption ◽  
Maximum Adsorption Capacity ◽  
Swelling Properties ◽  
Hydrogel Membrane ◽  
Calcium Alginate Hydrogel

In this paper, bovine serum albumin (BSA)-imprinted calcium alginate (CaAlg) hydrogel membrane was prepared using BSA as a template, sodium alginate (NaAlg) as a functional monomer, and CaCl2 as a cross-linker. The thickness of the CaAlg membrane was controlled by a glass rod enlaced with brass wires (the diameter was 0.1, 0.2, 0.3, 0.4, and 0.5 mm). The swelling properties of the CaAlg membranes prepared with different contents of NaAlg were researched. Circular dichroism indicated that the conformation of BSA did not change during the preparing and eluting process. The thinner the CaAlg hydrogel membrane was, the larger the adsorption capacity and the higher the imprinting efficiency of the CaAlg. The maximum adsorption capacity of molecularly imprinted polymer (MIP) and non-imprinted CaAlg hydrogel membrane (NIP) was 38.6 mg·g−1 and 9.2 mg·g−1, respectively, with an imprinting efficiency of 4.2. The MIP was loaded on the electrode to monitor the selective adsorption of BSA by voltammetry curve.

scholarly journals The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana)

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Márcia Regina Ribeiro Alves ◽  
Abraham Damian Giraldo Zuñiga ◽  
Rita de Cássia Superbi Sousa ◽  
Carmelita Zacchi Scolforo
Keyword(s):  
Bovine Serum Albumin ◽  
Zeta Potential ◽  
Serum Albumin ◽  
Adsorption Capacity ◽  
Bovine Serum ◽  
Serum Proteins ◽  
Second Order ◽  
Maximum Adsorption Capacity ◽  
Pseudo Second Order ◽  
Synthetic Hydroxyapatite

Bovine serum albumin is one of the major serum proteins; it plays an important role as a result of its functional and nutritional properties which have bioactive peptides. Adsorption method was used to separate protein, which involves hydroxyapatite, synthetic hydroxyapatite, and active babassu coal. Initially, characterization was carried out using the zeta potential of the adsorbents. Kinetic pseudo-first- and pseudo-second-order models were applied. For isotherms, equilibrium data studies were carried out using the Langmuir and Freundlich models, in addition to determining the efficiency of adsorptive process. The results of the zeta potential showed loads ranging from +6.9 to −42.8 mV. The kinetic data were better represented in the pseudo-second-order model with chemisorption characteristics. The adsorption capacity of the adsorbents decreased as pH increased, indicating that the electrostatic bonds and some functional groups of active babassu coal contributed to the reduction of adsorption, especially oxygen linked to carbon atoms. The value of pH 4.0 showed the best results of adsorption, being obtained as the maximum adsorption capacity (qm) and yield (%) (whereqm=87.95 mg g−1and 74.2%; 68.26 mg g−1and 68.6%; and 36.18 mg g−1, 37.4%) of hydroxyapatite, synthetic hydroxyapatite, and active babassu coal, respectively.

scholarly journals Removing Metal Ions from Water with Graphene–Bovine Serum Albumin Hybrid Membrane

Nanomaterials ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 276 ◽  
Author(s):  
Xiaoqing Yu ◽  
Shuwei Sun ◽  
Lin Zhou ◽  
Zhicong Miao ◽  
Xiaoyuan Zhang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Metal Ions ◽  
Metal Binding ◽  
Bovine Serum ◽  
Large Scale ◽  
Selective Adsorption ◽  
Hybrid Membrane ◽  
Transporter Protein ◽  
Metal Ions Detection

Here we report the fabrication of graphene oxide (GO)-based membranes covalently combined with bovine serum albumin (BSA) for metal ions detection. In this system, BSA acts as a transporter protein in the membrane and endows the membrane with selective recognition of Co2+, Cu2+, AuCl4−, and Fe2+. Combining the metal-binding ability of BSA and the large surface area of GO, the hybrid membrane can be used as a water purification strategy to selectively absorb a large amount of AuCl4− from HAuCl4 solution. Moreover, BSA could reduce the membrane-immobilized AuCl4− by adding sodium borohydride (NaBH4). Interestingly, adsorption experiments on three kinds of metal ions showed that the GO–BSA membrane had good selective adsorption of Co2+ compared with Cu2+ and Fe2+. The morphology and composition changes of the membrane were observed with atomic force microscopy (AFM) and Raman spectroscopy, respectively. It is expected that this facile strategy for fabricating large-scale graphene-biomolecule membranes will spark inspirations in the development of functional nanomaterials and wastewater purification.

scholarly journals Adsorption of bovine serum albumin (BSA) by bare magnetite nanoparticles with surface oxidative impurities that prevent aggregation

2019 ◽  
Vol 97 (8) ◽  
pp. 577-583 ◽  
Author(s):  
Somayeh Rahdar ◽  
Abbas Rahdar ◽  
Shahin Ahmadi ◽  
John F. Trant
Keyword(s):  
Biological Activity ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Magnetite Nanoparticles ◽  
Bovine Serum ◽  
Mammalian Cells ◽  
Particle Diameter ◽  
Resistant Bacteria ◽  
Maximum Adsorption Capacity ◽  
Corona Formation

Bare, uncoated magnetite nanoparticles, synthesized using an electrochemical surfactant-free synthesis, have highly oxidized surfaces that prevent aggregation. These particles have demonstrated highly intriguing biological activity showing extremely potent antibiotic activity against both gram-positive and gram-negative bacteria with little toxicity to rats. This difference in activity could be ascribed to the nature of the protein corona. The kinetics and thermodynamics of the binding of bovine serum albumin (BSA), used as a model serum protein, to these magnetite nanoparticles were analyzed. There is no significant change in particle diameter by dynamic light scattering following adsorption, indicating corona formation does not induce aggregation. The maximum adsorption capacity of the particles was determined to be 300 mg of BSA per gram of magnetite. The particles are able to adsorb 90% of the BSA at protein concentrations as high as 500 mg/L. The adsorption is best described using a pseudo second order model and a Langmuir Type III isotherm model. Thermodynamic analysis showed that the process is entropically driven and is spontaneous at all tested temperatures and conditions. However, it appears to be a weak to moderate physical adsorption. This moderate binding affinity could indicate the differential biological activity of these particles towards bacteria and mammalian cells and further support the contention that these are potentially useful new tools for targeting antibiotic-resistant bacteria.

Preparation and adsorption of bovine serum albumin-imprinted polyacrylamide hydrogel membrane grafted on non-woven polypropylene

Talanta ◽  
2014 ◽  
Vol 121 ◽  
pp. 256-262 ◽  
Author(s):  
Kongyin Zhao ◽  
Beibei Lin ◽  
Wenkui Cui ◽  
Lingzhi Feng ◽  
Tian Chen ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Polyacrylamide Hydrogel ◽  
Hydrogel Membrane

scholarly journals Bovine Serum Albumin Adsorption onto Hydroxyapatite and Biphasic Calcium Phosphate Ceramic Granules

2016 ◽  
Vol 721 ◽  
pp. 197-201 ◽  
Author(s):  
Inga Jurgelane ◽  
Armands Buss ◽  
Dagnija Loca
Keyword(s):  
Calcium Phosphate ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Adsorption Capacity ◽  
Bovine Serum ◽  
Biphasic Calcium Phosphate ◽  
Calcium Phosphate Ceramic ◽  
Beta Tricalcium Phosphate ◽  
Albumin Adsorption ◽  
Hydroxyapatite Formation

Calcium phosphate ceramics are one of the most studied biomaterials for bone substitution and regeneration. Bioactivity is one of the most important properties for these materials and it can be evaluated by adsorption of proteins and by hydroxyapatite formation on the surface in simulated body fluid (SBF). The aim of this work was to evaluate the bioactivity of ceramic granules with various hydroxyapatite (HAp) and beta tricalcium phosphate (β-TCP) ratios by adsorption of bovine serum albumin (BSA) in SBF and phosphate saline buffer (PBS) solution. The highest adsorption capacity in both solutions was observed for biphasic calcium phosphate sample with HAp/β-TCP ratio 50/50 but the lowest – for sample 10/90. The adsorption capacity of all samples was more than 2 times higher in SBF media than in PBS. The possibility of hydroxyapatite formation was evaluated by changes of Ca2+ concentration in SBF. After 5 days of incubation at 37oC all samples showed a decrease in Ca2+ concentration.

Imprinting of bovine serum albumin in a nonwoven polypropylene membrane supported polyacrylamide/calcium alginate interpenetrating polymer network hydrogel

RSC Advances ◽  
2014 ◽  
Vol 4 (99) ◽  
pp. 55846-55852 ◽  
Author(s):  
Bohong Kan ◽  
Beibei Lin ◽  
Kongyin Zhao ◽  
Xinxin Zhang ◽  
Lingzhi Feng ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Calcium Alginate ◽  
Polymer Network ◽  
Interpenetrating Polymer Network ◽  
Polyacrylamide Hydrogel ◽  
Hydrogel Film ◽  
Polypropylene Membrane

A non-woven polypropylene supported bovine serum albumin imprinted calcium alginate/polyacrylamide hydrogel film with good regeneration performance.

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