scholarly journals The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana)

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Márcia Regina Ribeiro Alves ◽  
Abraham Damian Giraldo Zuñiga ◽  
Rita de Cássia Superbi Sousa ◽  
Carmelita Zacchi Scolforo
Keyword(s):  
Bovine Serum Albumin ◽  
Zeta Potential ◽  
Serum Albumin ◽  
Adsorption Capacity ◽  
Bovine Serum ◽  
Serum Proteins ◽  
Second Order ◽  
Maximum Adsorption Capacity ◽  
Pseudo Second Order ◽  
Synthetic Hydroxyapatite

Bovine serum albumin is one of the major serum proteins; it plays an important role as a result of its functional and nutritional properties which have bioactive peptides. Adsorption method was used to separate protein, which involves hydroxyapatite, synthetic hydroxyapatite, and active babassu coal. Initially, characterization was carried out using the zeta potential of the adsorbents. Kinetic pseudo-first- and pseudo-second-order models were applied. For isotherms, equilibrium data studies were carried out using the Langmuir and Freundlich models, in addition to determining the efficiency of adsorptive process. The results of the zeta potential showed loads ranging from +6.9 to −42.8 mV. The kinetic data were better represented in the pseudo-second-order model with chemisorption characteristics. The adsorption capacity of the adsorbents decreased as pH increased, indicating that the electrostatic bonds and some functional groups of active babassu coal contributed to the reduction of adsorption, especially oxygen linked to carbon atoms. The value of pH 4.0 showed the best results of adsorption, being obtained as the maximum adsorption capacity (qm) and yield (%) (whereqm=87.95 mg g−1and 74.2%; 68.26 mg g−1and 68.6%; and 36.18 mg g−1, 37.4%) of hydroxyapatite, synthetic hydroxyapatite, and active babassu coal, respectively.

scholarly journals Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane

Polymers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 622 ◽  
Author(s):  
Meng Qi ◽  
Kongyin Zhao ◽  
Qiwen Bao ◽  
Peng Pan ◽  
Yuwei Zhao ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Adsorption Capacity ◽  
Bovine Serum ◽  
Calcium Alginate ◽  
Selective Adsorption ◽  
Maximum Adsorption Capacity ◽  
Swelling Properties ◽  
Hydrogel Membrane ◽  
Calcium Alginate Hydrogel

In this paper, bovine serum albumin (BSA)-imprinted calcium alginate (CaAlg) hydrogel membrane was prepared using BSA as a template, sodium alginate (NaAlg) as a functional monomer, and CaCl2 as a cross-linker. The thickness of the CaAlg membrane was controlled by a glass rod enlaced with brass wires (the diameter was 0.1, 0.2, 0.3, 0.4, and 0.5 mm). The swelling properties of the CaAlg membranes prepared with different contents of NaAlg were researched. Circular dichroism indicated that the conformation of BSA did not change during the preparing and eluting process. The thinner the CaAlg hydrogel membrane was, the larger the adsorption capacity and the higher the imprinting efficiency of the CaAlg. The maximum adsorption capacity of molecularly imprinted polymer (MIP) and non-imprinted CaAlg hydrogel membrane (NIP) was 38.6 mg·g−1 and 9.2 mg·g−1, respectively, with an imprinting efficiency of 4.2. The MIP was loaded on the electrode to monitor the selective adsorption of BSA by voltammetry curve.

A Precipitin-like Reaction of the Hemolymph of the Lobster Homarus americanus

1969 ◽  
Vol 26 (5) ◽  
pp. 1392-1397 ◽  
Author(s):  
James E. Stewart ◽  
Diane M. Foley
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Test Period ◽  
Fluorescent Material

The levels of fluorescent material in the hemolymph of lobsters injected with serum proteins from lobster hemolymph labelled with fluorescein remained relatively constant over a 6-day test period; the levels in lobsters injected with bovine serum albumin labelled with fluorescein declined rapidly. A precipitin-like reaction was observed when lobster hemolymph serum was titrated with bovine serum albumin in vitro.

Bovine Serum Albumin Adsorption onto Colloidal Al2O3Particles:  A New Model Based on Zeta Potential and UV−Vis Measurements

Langmuir ◽  
2004 ◽  
Vol 20 (23) ◽  
pp. 10055-10061 ◽  
Author(s):  
Kurosch Rezwan ◽  
Lorenz P. Meier ◽  
Mandana Rezwan ◽  
Janos Vörös ◽  
Marcus Textor ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Zeta Potential ◽  
Serum Albumin ◽  
Bovine Serum ◽  
New Model ◽  
Model Based ◽  
Bovine Serum Albumin Adsorption ◽  
Albumin Adsorption

scholarly journals Kinetic Study of the Bioadsorption of Methylene Blue on the Surface of the Biomass Obtained from the Algae D. antarctica

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Jhonatan R. Guarín ◽  
Juan Carlos Moreno-Pirajan ◽  
Liliana Giraldo
Keyword(s):  
Methylene Blue ◽  
Kinetic Study ◽  
Adsorption Capacity ◽  
Scientific Community ◽  
Second Order ◽  
Maximum Adsorption Capacity ◽  
Pseudo Second Order ◽  
Best Fit ◽  
Total Elimination ◽  
Purification Of Water

Currently, there is a great pollution of water by the dyes; due to this, several studies have been carried out to remove these compounds. However, the total elimination of these pollutants from the aquatic effluents has represented a great challenge for the scientific community, for which it is necessary to carry out investigations that allow the purification of water. In this work, we studied the bioadsorption of methylene blue on the surface of the biomass obtained from the algae D. antarctica. This material was characterized by SEM and FTIR. To the data obtained in the biosorption experiments, different models of biosorption and kinetics were applied, finding that the best fit to the obtained data is given by applying the pseudo-second-order models and the Toth model, respectively. It was also determined that the maximum adsorption capacity of MB on the surface of the biomass is 702.9 mg/g, which shows that this material has great properties as a bioadsorbent.

Development and creation of zeta potential reference material of the particles in a liquid medium

2021 ◽  
pp. 58-62
Author(s):  
Dmitrii I. Belenkii ◽  
Dmitry V. Averkin ◽  
Dmitry V. Vishnevetskii ◽  
Svetlana D. Khizhnyak ◽  
Pavel M. Pakhomov
Keyword(s):  
Bovine Serum Albumin ◽  
Zeta Potential ◽  
Serum Albumin ◽  
Aqueous Solutions ◽  
Bovine Serum ◽  
Polystyrene Latex ◽  
Powder Materials ◽  
Silver Acetate ◽  
Supramolecular Systems ◽  
Standard Samples

A comprehensive study to develop and create standard samples of the zeta potential unit of particles in a liquid in accordance with the requirements of GOST ISO 13099-2-2016 in the range of measured values from minus 150 mV to plus 150 mV was carried out. The existing standard samples of zeta potential in Russia and the world have been investigated; the analysis of the applicability of the starting materials and components for the creation of new standard samples has done. Samples based on modified polystyrene latex microspheres, bovine serum albumin and supramolecular systems based on aqueous solutions of L-cysteine, N-acetylcysteine, and silver acetate were considered as promising standard samples of the zeta potential of particles. All studies were carried out on equipment from the State Primary Standard of Dispersed Parameters of Aerosols, Suspensions and Powder Materials GET 163-2020 using the method of electrophoretic light scattering and measuring the pH value. It was found that polystyrene latex microspheres do not correspond to the requirements of the work in terms of developing a set of standard samples. Samples based on suspensions of bovine serum albumin do not correspond to the requirements of GOST ISO 13099-2-2016. Supramolecular systems based on aqueous solutions of L-cysteine, N-acetylcysteine, and silver acetate were proposed as standard samples. Such systems are characterized by the ability to modify the initial potential-determining layer of particles without changing their aggregate stability. Studies of the long-term stability of the developed standard samples of the zeta-potential unit of particles in a liquid have been carried out.

Kinetics of lead ion biosorption from aqueous solution onto lyophilized Aspergillus niveus

2010 ◽  
Vol 5 (1) ◽  
Author(s):  
Hülya Karaca ◽  
Turgay Tay ◽  
Merih Kıvanç
Keyword(s):  
Adsorption Capacity ◽  
Correlation Coefficients ◽  
Freundlich Isotherm ◽  
Second Order ◽  
Lead Ions ◽  
Lead Ion ◽  
Maximum Adsorption Capacity ◽  
Order Kinetic ◽  
Aspergillus Niveus ◽  
Pseudo Second Order

The biosorption of lead ions (Pb2+) onto lyophilized fungus Aspergillus niveus was investigated in aqueous solutions in a batch system with respect to pH, contact time and initial concentration of the ions at 30 °C. The maximum adsorption capacity of lyophilized A. niveus was found to be 92.6 mg g−1 at pH 5.1 and the biosorption equilibrium was established about in 30 min. The adsorption capacity obtained is one of the highest value among those reported in the literature. The kinetic data were analyzed using the pseudo-first-order kinetic, pseudo-second-order kinetic, and intraparticle diffusion equations. Kinetic parameters, such as rate constants, equilibrium adsorption capacities, and related correlation coefficients for the kinetic models were calculated and discussed. It was found that the adsorption of lead ions onto lyophilized A. niveus biomass fit the pseudo-second-order kinetic model well. The Langmuir and Freundlich isotherm parameters for the lead ion adsorption were applied and the Langmuir model agreed better with the adsorption of lead ions onto lyophilized A. niveus.

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