Imprinting of bovine serum albumin in a nonwoven polypropylene membrane supported polyacrylamide/calcium alginate interpenetrating polymer network hydrogel

RSC Advances ◽  
2014 ◽  
Vol 4 (99) ◽  
pp. 55846-55852 ◽  
Author(s):  
Bohong Kan ◽  
Beibei Lin ◽  
Kongyin Zhao ◽  
Xinxin Zhang ◽  
Lingzhi Feng ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Calcium Alginate ◽  
Polymer Network ◽  
Interpenetrating Polymer Network ◽  
Polyacrylamide Hydrogel ◽  
Hydrogel Film ◽  
Polypropylene Membrane

A non-woven polypropylene supported bovine serum albumin imprinted calcium alginate/polyacrylamide hydrogel film with good regeneration performance.

Preparation and adsorption of bovine serum albumin-imprinted polyacrylamide hydrogel membrane grafted on non-woven polypropylene

Talanta ◽  
2014 ◽  
Vol 121 ◽  
pp. 256-262 ◽  
Author(s):  
Kongyin Zhao ◽  
Beibei Lin ◽  
Wenkui Cui ◽  
Lingzhi Feng ◽  
Tian Chen ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Polyacrylamide Hydrogel ◽  
Hydrogel Membrane

scholarly journals Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane

Polymers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 622 ◽  
Author(s):  
Meng Qi ◽  
Kongyin Zhao ◽  
Qiwen Bao ◽  
Peng Pan ◽  
Yuwei Zhao ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Adsorption Capacity ◽  
Bovine Serum ◽  
Calcium Alginate ◽  
Selective Adsorption ◽  
Maximum Adsorption Capacity ◽  
Swelling Properties ◽  
Hydrogel Membrane ◽  
Calcium Alginate Hydrogel

In this paper, bovine serum albumin (BSA)-imprinted calcium alginate (CaAlg) hydrogel membrane was prepared using BSA as a template, sodium alginate (NaAlg) as a functional monomer, and CaCl2 as a cross-linker. The thickness of the CaAlg membrane was controlled by a glass rod enlaced with brass wires (the diameter was 0.1, 0.2, 0.3, 0.4, and 0.5 mm). The swelling properties of the CaAlg membranes prepared with different contents of NaAlg were researched. Circular dichroism indicated that the conformation of BSA did not change during the preparing and eluting process. The thinner the CaAlg hydrogel membrane was, the larger the adsorption capacity and the higher the imprinting efficiency of the CaAlg. The maximum adsorption capacity of molecularly imprinted polymer (MIP) and non-imprinted CaAlg hydrogel membrane (NIP) was 38.6 mg·g−1 and 9.2 mg·g−1, respectively, with an imprinting efficiency of 4.2. The MIP was loaded on the electrode to monitor the selective adsorption of BSA by voltammetry curve.

The Rebinding Properties of Bovine Serum Albumin Imprinted Calcium Alginate/Phosphate Hybrid Microspheres Via the Adjustment of pH Values and Salt Concentration

2010 ◽  
Vol 297 (1) ◽  
pp. 126-137 ◽  
Author(s):  
Kongyin Zhao ◽  
Guoxiang Cheng ◽  
Junfu Wei ◽  
Jinyang Zhou ◽  
Jinlong Zhang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Salt Concentration ◽  
Bovine Serum ◽  
Calcium Alginate ◽  
Ph Values

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

Stability of Prostacyclin in Human Plasma and Whole Blood: Studies on the Protective Effect of Albumin

1981 ◽  
Vol 46 (03) ◽  
pp. 645-647 ◽  
Author(s):  
M A Orchard ◽  
C Robinson
Keyword(s):  
Platelet Aggregation ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protective Effect ◽  
Whole Blood ◽  
Bovine Serum ◽  
Fatty Acid Content ◽  
Krebs Solution ◽  
Antiaggregatory Activity ◽  
Free Plasma

SummaryThe biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37°C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.

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