scholarly journals Antioxidant and Antiproliferative Potentials of Ficus glumosa and Its Bioactive Polyphenol Metabolites

2021 ◽  
Vol 14 (3) ◽  
pp. 266
Author(s):  
Moses Mutuse Mutungi ◽  
Felix Wambua Muema ◽  
Festus Kimutai ◽  
Yong-Bing Xu ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Stem Bark ◽  
Background Information ◽  
Antioxidant Power ◽  
Chemical Profiling ◽  
Anti Cancer ◽  
Ic50 Values ◽  
Ferric Reducing Antioxidant Power ◽  
High Cytotoxicity ◽  
Ethanol Extracts

Ficus glumosa Delile (Moraceae), a reputed plant that is used in herbal medicine, is of high medicinal and nutritional value in local communities primarily ascribed to its phytochemical profile. Currently, there are hardly any fine details on the chemical profiling and pharmacological evaluation of this species. In this study, the flavonoids and phenolics contents of the ethanol extracts and four extracted fractions (petroleum ether (PE), ethyl acetate (EA), n-butanol, and water) of the stem bark of Ficus glumosa were firstly quantified. Further, their antioxidant and antiproliferative potentials were also evaluated. The quantitative determination indicated that the EA and n-butanol fractions possessed the highest total flavonoids/phenolics levels of 274.05 ± 0.68 mg RE/g and 78.87 ± 0.97 mg GAE/g, respectively. Similarly, for the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric-reducing antioxidant power (FRAP) assays, the EA fraction exhibited high potency in both DPPH and ABTS+ scavenging activities with IC50 values of 0.23 ± 0.03 mg/mL, 0.22 ± 0.03 mg/mL, and FRAP potential of 2.81 ± 0.01 mg Fe2+/g, respectively. Furthermore, the EA fraction displayed high cytotoxicity against human lung (A549) and colon (HT-29) cancer cells. Additionally, the liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) was employed in order to characterize the chemical constituents of the EA fraction of Ficus glumosa stem bark. Our findings revealed 16 compounds from the EA fraction that were possibly responsible for the strong antioxidant and anti-proliferative properties. This study provides edge-cutting background information on the exploitation of Ficus glumosa as a potential natural antioxidant and anti-cancer remedy.

scholarly journals Comparison of Phenolic Contents and Scavenging Activities of Miang Extracts Derived from Filamentous and Non-Filamentous Fungi-Based Fermentation Processes

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1144
Author(s):  
Aliyu Dantani Abdullahi ◽  
Pratthana Kodchasee ◽  
Kridsada Unban ◽  
Thanawat Pattananandecha ◽  
Chalermpong Saenjum ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Protective Effects ◽  
Tea Leaves ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Ic50 Values ◽  
Ferric Reducing Antioxidant Power ◽  
The Impact

The study investigated the impact of the fermentation process on the phenolic contents and antioxidant and anti-inflammatory activities in extracts of Miang, an ethnic fermented tea product of northern Thailand. The acetone (80%) extraction of Miang samples fermented by a non-filamentous fungi-based process (NFP) and filamentous fungi-based process (FFP) had elevated levels of total polyphenols, total tannins, and condensed tannins compared to young and mature tea leaves. The antioxidant studies also showed better the half-maximal inhibitory concentration (IC50) values for fermented leaves in both 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity assays as well as improved ferric reducing antioxidant power (FRAP) compared to young and mature tea leaves. Extracts of NFP and FFP samples at concentrations of 50 and 100 ppm showed better protective effects against hydrogen peroxide (H2O2)-induced intracellular reactive oxygen species (ROS) production in HT-29 colorectal cells without exerting cytotoxicity. Additionally, lipopolysaccharide (LPS)-induced production of nitric oxide (a proinflammatory mediator as well as a reactive nitrogen species) was also inhibited by these fermented Miang extracts with an IC50 values of 17.15 μg/mL (NFP), 20.17 μg/mL (FFP), 33.96 μg/mL (young tea leaves), and 31.33 μg/mL (mature tea leaves). Therefore, both NFP-Miang and FFP-Miang showed the potential to be targeted as natural bioactive functional ingredients with preventive properties against free radical and inflammatory-mediated diseases.

scholarly journals Changes of Phytochemical Components (Urushiols, Polyphenols, Gallotannins) and Antioxidant Capacity during Fomitella fraxinea–Mediated Fermentation of Toxicodendron vernicifluum Bark

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 683 ◽  
Author(s):  
Da-Ham Kim ◽  
Min-Ji Kim ◽  
Dae-Woon Kim ◽  
Gi-Yoon Kim ◽  
Jong-Kuk Kim ◽  
...  
Keyword(s):  
Fermentation Process ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Antioxidant Power ◽  
Food Ingredients ◽  
Ferric Reducing Antioxidant Power ◽  
Quantitative Changes ◽  
Phytochemical Components ◽  
Toxicodendron Vernicifluum

The stem bark of Toxicodendron vernicifluum (TVSB) has been widely used as a traditional herbal medicine and food ingredients in Korea. However, its application has been restricted due to its potential to cause allergies. Moreover, there is limited data available on the qualitative and quantitative changes in the composition of its phytochemicals during fermentation. Although the Formitella fraxinea-mediated fermentation method has been reported as an effective detoxification tool, changes to its bioactive components and the antioxidant activity that takes place during its fermentation process have not yet been fully elucidated. This study aimed to investigate the dynamic changes of urushiols, bioactive compounds, and antioxidant properties during the fermentation of TVSB by mushroom F. fraxinea. The contents of urushiols, total polyphenols, and individual flavonoids (fisetin, fustin, sulfuretin, and butein) and 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG) significantly decreased during the first 10 days of fermentation, with only a slight decrease thereafter until 22 days. Free radical scavenging activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6- sulfonic acid) (ABTS), and ferric reducing/antioxidant power (FRAP) as an antioxidant function also decreased significantly during the first six to nine days of fermentation followed by a gentle decrease up until 22 days. These findings can be helpful in optimizing the F. fraxinea–mediated fermentation process of TVSB and developing functional foods with reduced allergy using fermented TVSB.

scholarly journals Isolation of Lasiodiplodin and Evaluation of some Biological Activities of the Stem Barks of Phyllanthus Albizzioides (Kurz) Hook.f.

2019 ◽  
Vol 1 (4) ◽  
pp. 199-216 ◽  
Author(s):  
Khine Zar Wynn Lae ◽  
Su Swe Su ◽  
Nwet Nwet Win ◽  
Ni Ni Than ◽  
Hla Ngwe
Keyword(s):  
Biological Activities ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
2D Nmr ◽  
Stem Bark ◽  
Chloroform Extract ◽  
Traditional System ◽  
Spectroscopic Analyses ◽  
Ethanol Extracts ◽  
Relative Structure

A large number of the plants are claimed to possess the antibiotic properties in the traditional system and are also used extensively by the tribal people throughout the world. It is now mostly thought that nature has given the cure of every disease in one form or another. Plants have been known to cure people from various diseases in Ayurveda. This research focused on the screening of phytochemicals and some biological activities of Phyllanthus albizzioides. The research showed that the ethanol extracts were found to be greater chemical constituents than watery extracts. Among the chemical constituents, steroid compounds were observed in highest amount in ethanol extract and the second highest in flavonoid compounds followed by tannin compounds and pheolic compounds. Furthermore, the ethanol extracts were more effective than the watery extracts in all tested biological activities such as antioxidant, anti-diabetic, cytotoxicity, antimicrobial, antitumor and NO inhibition activities. A cyclohexanone compound was isolated from the chloroform extract of the stem bark of P. albizzioides. The relative structure was determined to be 4,5-dihydroxy-3-methyl-cyclohex-2-enone on the basic of HRESIMS, 1H NMR and 13C NMR spectroscopic analyses, including 2D NMR experiments.

scholarly journals Phenolic Content, Antioxidant and 15-Lypoxygenase Inhibitory Effects of Daniellia oliveri (rolfe) Hutch. & Dalziel (Fabaceae)

2021 ◽  
pp. 10-18
Author(s):  
Mariam Traore ◽  
Adjaratou C. Coulibaly ◽  
Aboubacar Yaro ◽  
Esther W. L. M. Kabre ◽  
Noufou Ouedraogo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Stem Bark ◽  
Inhibitory Effect ◽  
Phytochemical Screening ◽  
Methanol Fraction ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Daniellia Oliveri

Aims: The aim of this study is to evaluate the content of phenolic compounds, antioxidant and 15-lipoxygenase inhibition effects of methanolic extract and its fractions of Daniellia. oliveri stem bark. Place and Duration of Study: The work was carried out in the Department of Traditional Pharmacopoeia and Pharmacy (MEPHATRA / PH) of the Institute for Research in Health Sciences (IRSS) and LABIOCA from Université Joseph KI-ZERBO in Ouagadougou between February 2018 and June 2019 Methodology: The phytochemical screening and phenolic content were determined by the methods described in the literature. Antioxidant activity was determined by DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,20-azinobis 3-ethylbenzoline-6-sulphonate), FRAP (ferric reducing antioxidant power), and lipid peroxidation (LPO). The anti-inflammatory activities were measured on the ability of the extract to inhibit the activity of enzymes such as 15-lipoxygenase Results: Phytochemical screening revealed the presence of flavonoids, tannins, saponins, anthocyanosides, triterpenes and sterols. The extract and its fractions showed a good content of phenolic compounds with values ranging from 773.97 ± 22.91 to 505.51 mg EAG / g for total polyphenols; from 24.38 ± 0.34 to 12.04 ± 0.08 mg EQ / g in total flavonoids, 311.65 ± 6.70 to 221 ± 0.52 mg EC / g in condensed tannins; 681.81 ± 3.97 to 421.29 ± 8.73 mg EUA / g in total triterpenes and from 280.29 ± 21.72 to 222.04 ± 20.99 mg EC / g for total sterols. The methanol fraction showed the best antioxidant activity as well as the best inhibitory effect on 15-lipoxygenase activity. Conclusion: These results showed that Daniellia. oliveri possessed an antioxidant power, an inhibitory effect of 15 lipoxygenase. Daniellia oliveri could be used in the management of inflammatory pathologies and oxidative stress.

scholarly journals Screening of In Vitro Health Benefits of Tangerine Tomatoes

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 230 ◽  
Author(s):  
Hartono Tanambell ◽  
Siew Young Quek ◽  
Karen Suzanne Bishop
Keyword(s):  
Scientific Evidence ◽  
Health Benefits ◽  
Beta Carotene ◽  
Antioxidant Power ◽  
Chicken Egg ◽  
Ic50 Values ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory ◽  
Prevention Of Cancer

Tomatoes have been associated with various health benefits, including the prevention of chronic diseases. The cis-isomers of lycopene occurring in tangerine tomatoes were, through clinical trials, proven to be more bioavailable than the all-trans lycopene found in red tomatoes. Nonetheless, scientific evidence regarding the bioactivities of the tangerine tomatoes is lacking. In this article, the antioxidant, anticancer, and anti-inflammatory properties of extracts prepared from four different tomato varieties, namely Alfred, Olga’s Round Golden Chicken Egg, Golden Green, and Golden Eye, were investigated. While the antioxidant capacities of the extracts were measured through the ferric reducing antioxidant power (FRAP) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, their anti-proliferative properties in prostate cancer cell lines were examined through the Sulforhodamine-B (SRB) assay. The anti-inflammatory activities of the extracts were assessed through the toll-like receptor (TLR)2, TLR4, and nucleotide-binding oligomerization domain containing protein 2 (NOD2)-mediated inflammatory pathways. Our results show that the tangerine tomatoes had lower IC50 values in both the anticancer and anti-inflammatory assays compared to the red tomatoes. Specifically, the half-maximal inhibitory concentration (IC50) values of the tangerine tomatoes in LNCaP cells were approximately two to three fold lower than the red tomato (IC50: 14.46, 5.62, and 8.08 mg dry tomato equivalent/mL from Alfred hexane-acetone, Olga’s Round Golden Chicken Egg hexane, and Golden Green hexane, respectively). These findings indicate that the tangerine varieties, Olga’s Round Golden Chicken Egg and Golden Green, possess greater potential to be used in conjunction with treatment and for the prevention of cancer and inflammatory-related diseases than the Alfred (red) and Golden Eye (high beta-carotene) varieties.

scholarly journals Comparative Phytochemical Profile and Biological Activity of Four Major Medicinal Halophytes from Qassim Flora

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2208
Author(s):  
Hamdoon A. Mohammed ◽  
Hussein M. Ali ◽  
Kamal A. Qureshi ◽  
Mansour Alsharidah ◽  
Yasser I. Kandil ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Biological Properties ◽  
Human Pancreatic Cancer ◽  
Antioxidant Power ◽  
Hydroalcoholic Extract ◽  
Anabasis Articulata ◽  
Ic50 Values ◽  
Ferric Reducing Antioxidant Power ◽  
Induced Apoptosis

Four halophytic plants, Lycium shawii, Anabasis articulata, Rumex vesicarius, and Zilla spinosa, growing in the central Qassim area, Saudi Arabia, were phytochemically and biologically investigated. Their hydroalcoholic extracts’ UPLC-ESIQ-TOF analyses demonstrated the presence of 44 compounds of phenolic acids, flavonoids, saponins, carbohydrates, and fatty acids chemical classes. Among all the plants’ extracts, L. shawii showed the highest quantities of total phenolics, and flavonoids contents (52.72 and 13.01 mg/gm of the gallic acid and quercetin equivalents, respectively), along with the antioxidant activity in the TAA (total antioxidant activity), FRAP (ferric reducing antioxidant power), and DPPH-SA (2,2-diphenyl-1-picryl-hydrazyl-scavenging activity) assays with 25.6, 56.68, and 19.76 mg/gm, respectively, as Trolox equivalents. The hydroalcoholic extract of the L. shawii also demonstrated the best chelating activity at 21.84 mg/gm EDTA equivalents. Among all the four halophytes, the hydroalcoholic extract of L. shawii exhibited the highest antiproliferative activity against MCF7 and K562 cell lines with IC50 values at 194.5 µg/mL and 464.9 µg/mL, respectively. The hydroalcoholic extract of A. articulata demonstrated better cytotoxic activity amongst all the tested plants’ extracts against the human pancreatic cancer cell lines (PANC1) with an IC50 value of 998.5 µg/mL. The L. shawii induced apoptosis in the MCF7 cell lines, and the percentage of the necrotic cells changed to 28.1% and 36.5% for the IC50 and double-IC50 values at 22.9% compared with the untreated groups. The hydroalcoholic extract of L. shawii showed substantial antibacterial activity against Bacillus cereus ATCC 10876 with a MIC value of 12.5 mg/mL. By contrast, the A. articulata and Z. spinosa exhibited antifungal activities against Aspergillus niger ATCC 6275 with MIC values at 12.5 and 50 mg/mL, respectively. These findings suggested that the L. shawii is a potential halophyte with remarkable biological properties, attributed to its contents of phenolics and flavonoid classes of compounds in its extract.

Secondary Metabolites from the Barks of Eucommia ulmoides and Their Bioactivities

2019 ◽  
Vol 41 (1) ◽  
pp. 169-169
Author(s):  
Wen Yan Liu Wen Yan Liu ◽  
Zi Ling Zi Ling ◽  
Dong Dong ◽  
Ke Huan Li Ke Huan Li ◽  
Jian Hua Shao Jian Hua Shao ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
2D Nmr ◽  
Eucommia Ulmoides ◽  
Antioxidant Power ◽  
Ic50 Values ◽  
Ferric Reducing Antioxidant Power ◽  
Intense Activity

Eucommia ulmoides Oliv., exhibiting diverse bioactivities including antimicrobial, antioxidant, neuroprotective, anti-tumor and anti-inflammatory properties, has long been known as an active ingredient commonly used in antihypertensive herbal prescriptions in China. The 95% ethanol extract of the barks of E. ulmoides showed antimicrobial and antioxidant activities and then was isolated by bioactivity-guided fractionation to obtain a new cinnamyl glucoside, 1-O-trans-cinnamoyl-β-D-apiofuranosyl-(1→6)-β-D-glucopyranose (1), together with twenty known secondary metabolites (2–21). And their structures were identified by extensive spectroscopic analyses, including 1D and 2D NMR, UV, IR and HRESIMS, and chemical methods. All secondary metabolites were evaluated for their antimicrobial and antioxidant activities in vitro. Compounds 7, 8, 11, and 21 exhibited moderately antimicrobial activity. In the antioxidant activity assay, compounds 3, 7, 11, and 14 showed intense activity in ferric reducing antioxidant power (FRAP) with the values of 9.82 mmol/g, 19.25 mmol/g, 14.52 mmol/g, and 12.58 mmol/g in comparison to Trolox (9.02 mmol/g). And compounds 2, 11, and 14–16 showed more potent activity with IC50 values ranging from 18.34 to 24.36 M than Trolox (26.35 M) in ABTS+• scavenging assay.

Antioxidant Activities of Ethanol Extracts from Zanthoxylum avicennae

2014 ◽  
Vol 472 ◽  
pp. 801-804
Author(s):  
Xi Feng Li ◽  
Nan Nan Zheng ◽  
Lu Tang ◽  
Wei Xiao Zhang ◽  
You Xu Wang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Ethanol Extracts ◽  
Dpph Radical Scavenging

The Zanthoxylum avicennae 95% EtoH extracts after concentration were dissolved in H2O and extracted with EtoAc and n-BuOH Successively. The n-BuOH layer was added to Diaion HP-20 macroporous resin column, then the resin was washed by distilled water to get rid of impurity, then washed by 20%, 40% and 60% methanol individually, and obtained M20, M40and M60respectively. And antioxidant activity was assessed using three methods: DPPH radical-scavenging activity, ABTS+radical-scavenging activity, ferric reducing antioxidant power (FRAP) assay. The results indicate that TEAC value of M40were higher than other part extracted from Zanthoxylum avicennae 95% EtoH extracts during three methods .The results of the experiments also can find M40is the strongest antioxidant activity part in Zanthoxylum avicennae 95% EtoH extracts and provide reference for further isolating the part M40.

scholarly journals Antioxidant and Anti-Inflammatory Activities of the Crude Extracts of Moringa oleifera from Kenya and Their Correlations with Flavonoids

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 296 ◽  
Author(s):  
Yong-Bing Xu ◽  
Gui-Lin Chen ◽  
Ming-Quan Guo
Keyword(s):  
Moringa Oleifera ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Reducing Power ◽  
No Production ◽  
Antioxidant Power ◽  
Crude Extracts ◽  
Ic50 Values ◽  
Anti Inflammatory

Moringa oleifera Lam. (M. oleifera) is commonly distributed and utilized in tropical and sub-tropical areas. There has been a large number of reports on the antioxidant and anti-inflammatory activity of its leaves, but only a few about its seeds and roots. Hence, in this work we aimed to systematically compare the antioxidant and anti-inflammatory activities of the ethanol crude extracts of leaves, seeds, and roots of M. oleifera from Kenya, and further correlate the differential activities with the chemical constituents from these three parts. The antioxidant activities were measured by using three different assays (DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) and FRAP (Ferric-Reducing Antioxidant Power), respectively). Results showed that the leaf extracts displayed the highest DPPH radical scavenging and FRAP total reducing power activities with IC50 values of 1.02 ± 0.13 mg/mL and 0.99 ± 0.06 mM Fe2+/g, respectively; the leaf and root extracts exhibited potential ABTS radical scavenging activities with the IC50 values of 1.36 ± 0.02 and 1.24 ± 0.03 mg/mL. Meanwhile, the leaf and seed extracts (11.1–100 µg/mL) also exerted obvious anti-inflammatory activities, as indicated by the inhibition of NO production. To further reveal correlations between these differential activities with the chemical constituents in the three organs, the total flavonoids content (TFC) of the three different extracts were evaluated, and the TFC of leaves, seeds and roots were found to be 192.36 ± 2.96, 5.89 ± 0.65 and 106.79 ± 2.12 mg rutin equivalent (RE)/g, respectively. These findings indicated the important impacts of the total flavonoid contents on antioxidant and anti-inflammatory activities. Additionally, we further determined the phytochemical profiles of M. oleifera by HPLC-UV/ESI-MS/MS, and identified most of the chemical constituents of leaves as flavonoids. In summary, the leaves of M. oleifera are a better potential natural source of antioxidants and anti-inflammatory agents, and very promising for development into the health promoting dietary supplements.

scholarly journals EVALUATION OF IN VITRO- ANTI-OXIDANT POTENTIAL OF AQUEOUS ROOT EXTRACT OF CLERODENDRUM SERRATUM L.

2017 ◽  
Vol 10 (4) ◽  
pp. 402
Author(s):  
Raj Kumar Tiwari ◽  
Udayabanu Malairaman ◽  
Silpi Chanda
Keyword(s):  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Root Extract ◽  
Antioxidant Power ◽  
Ic50 Values ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging ◽  
Aqueous Root Extract

Objective: The intent  of this report  was to investigate the effect of aqueous root extract of Clerodendrum serratum L. for antioxidant activity using divergent models viz. DPPH scavenging assay, Superoxide scavenging assay and Ferric Reducing Antioxidant Power (FRAP) assay.Materials and Methods: The root of C. serratum was extracted using water. The yield of aqueous extract was 10%w/w. The outcome was examined statistically by the regression method.Results and discussions: The IC50 values are 85.43 µg/ml and 107.59 µg/ml for DPPH radical scavenging and Superoxide scavenging assay respectively whereas  FRAP showed significant reducing power activity with increased concentration of sample. The pilot study showed, a significant correlation existed between concentrations of the extract and percentage engrossment of free radicals.Conclusion: The antioxidant property may be corresponding to the polyphenols and flavonoids adjacent in the extract. These results clearly revealed that C. serratum might be effective against diseases analogous with free radical mediated. Keywords Clerodendrum serratum, DPPH, Superoxide, FRAP, Rutin, Antioxidant

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