scholarly journals Molecular Investigation of Porcine Circovirus Type 3 Infection in Pigs in Namibia

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 585
Author(s):  
Umberto Molini ◽  
Giuseppe Marruchella ◽  
Frieda Matheus ◽  
Yvonne Maria Hemberger ◽  
Bernard Chiwome ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Swine Industry ◽  
Domestic Pig ◽  
Pig Production ◽  
Molecular Investigation ◽  
Low Levels ◽  
Type 3 ◽  
Production Facilities

Porcine circovirus type 3 (PCV-3) infection is widely distributed in domestic pig populations in America, Europe, and Asia. However, no data is currently available about its presence and distribution in Africa. This study investigated the presence of PCV-3 in pigs (n = 122) in Namibia, by means of biomolecular methods. The pig samples collected (n = 122) were representative of the swine industry in Namibia, covering the major pig production facilities in the country. All of the samples tested were negative for PCV-3, and this indicated that the virus was either not present in the country or was circulating at low levels. Further studies are needed to better understand the distribution, if any, of PCV-3 in Namibia.

scholarly journals Nucleolar Phosphoprotein NPM1 Interacts With Porcine Circovirus Type 3 Cap Protein and Facilitates Viral Replication

2021 ◽  
Vol 12 ◽  
Author(s):  
Jiangwei Song ◽  
Lei Hou ◽  
Dan Wang ◽  
Li Wei ◽  
Shanshan Zhu ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Mass Spectrometry Analysis ◽  
Cellular Proteins ◽  
Porcine Circovirus ◽  
Amino Acid Residues ◽  
Liquid Chromatography Mass Spectrometry ◽  
Swine Industry ◽  
Spectrometry Analysis ◽  
Infected Cells ◽  
Type 3

Porcine circovirus type 3 (PCV3) is a recently discovered virus with potentially significant implications on the global swine industry. PCV3 replication involves the entry of the viral capsid (Cap) protein with nucleolar localization signals into the nucleus. Using liquid chromatography-mass spectrometry analysis, nucleolar phosphoprotein NPM1 was identified as one of the cellular proteins bound to PCV3 Cap. Co-immunoprecipitation demonstrated that PCV3 Cap interacts directly with NPM1, where the region binding with NPM1 is mapped to amino acid residues 1–38 of Cap. Upon co-transfection, the expression of Cap protein promoted the redistribution of NPM1, which translocated from the nucleus to the cytoplasm and colocalized with Cap in cultured PK15 cells. NPM1 expression was upregulated and translocated from the nucleus to the cytoplasm in PCV3-infected cells, upon siRNA-mediated depletion, or upon treatment with NPM1 inhibitor in PK15 cells with impaired PCV3 replication, as evidenced by decreased levels of viral DNA synthesis and protein expression. By contrast, the replication of PCV3 was enhanced in stably NPM1-expressing cells via a lentivirus-delivered system. Taken together, these findings indicate that NPM1 interacts with PCV3 Cap and plays a crucial role in PCV3 replication.

scholarly journals Molecular detection of porcine circovirus type 3 in Shanxi Province, China

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Weidong Yue ◽  
Yihui Liu ◽  
Xinrong Zhang ◽  
Haili Ma ◽  
Junping He
Keyword(s):  
Genomic Sequence ◽  
The United States ◽  
Porcine Circovirus Type ◽  
Shanxi Province ◽  
Porcine Circovirus ◽  
Swine Industry ◽  
Double Positive ◽  
Potential Threat ◽  
Tissue Samples ◽  
Type 3

AbstractPorcine circovirus type 3 (PCV3), which was first detected in the United States of America in 2015, is a potential threat to the swine industry. However, the prevalence of PCV3 in Shanxi Province, China, is unclear. In this research, the prevalence and genetic diversity of PCV3 were investigated in above area. Lung tissue samples (n = 491) from 19 pig slaughterhouses across 11 cities throughout Shanxi Province were analyzed for PCV3 infection by PCR in 2019. The results showed that PCV3 positive rates in slaughterhouses and individuals were 100% (19/19) and 86.76% (426/491), respectively. PCV2 and PCV3 double-positive rates in slaughterhouses and individuals were 100% (19/19) and 59.27% (291/491), respectively. PCR positive samples were further sequenced and 8 PCV3 isolates were identified. The nucleotide homology of these isolates with other PCV3 isolates in NCBI database was 97.45–99.90%. A phylogenetic analysis, based on the complete genomic sequence and ORF2, divided these PCV3 strains into 2 major groups. Based on A24/V and R27/K amino acid mutations of capsid protein, the 8 identified PCV3 strains were separated to 2 clades. This was the first detailed investigation into the epidemiology of PCV3 in Shanxi Province. Our findings enabled us to assess the possibility of widespread transmission from this region. Thus, current findings establish a basis for further studies of genetic variations in PCV3 strains circulating in China.

The presence and genetic characteristics of porcine circovirus 3 from pigs in Southern and Central provinces of Vietnam

2020 ◽  
pp. 26-31
Author(s):  
Giao N. P. Trinh
Keyword(s):  
Gene Segment ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Nucleotide Sequencing ◽  
Duplex Pcr ◽  
Serum Samples ◽  
Swine Industry ◽  
Genetic Characteristics ◽  
Swine Herds ◽  
Type 3

Porcine circovirus type 3 (PCV3) is an emerging circovirus species that has recently been reported in different countries around the world, suggesting a widespread circulation. This study was carried out in order to investigate the presence and further genetic characteristics of PCV3 from swine herds in Southern and Central provinces of Vietnam. A duplex PCR assay for rapid detection of PCV3 in pigs was established with a pair of specific primers designed between rep and cap gene segment to amplify full-length ORF2 and another set of primers binding to COX1gene serving as an internal amplification control (IAC). The resulting duplex PCR was used to examine PCV3 presence in 94tissue and serum samples. Subsequently, PCV3 was detected in 10 out of 94 cases (10.6%). The infection rate in sows (14.3%) was higher than that in grower pigs (7.7%). Regarding nucleotide sequence comparison, 10 ORF2 genes were selected for nucleotide sequencing and their alignment showed 97.2% - 99.5% homology. According to the phylogenetic analysis and sequence alignment of cap gene, all the sequences were clustered into group PCV3a,including 9 strains of sub-group PCV3a1 and only one strain of subgroup PCV3a2. These findings indicated that the PCV3a group is circulating in swine farms in Vietnam. This study provides better insights into epidemiology of this pathogen in the national swine industry.

Production of a monoclonal antibody against Porcine circovirus type 3 cap protein

2018 ◽  
Vol 261 ◽  
pp. 10-13 ◽  
Author(s):  
Xiangdong Li ◽  
Yilin Bai ◽  
Haiyang Zhang ◽  
Dingding Zheng ◽  
Tongyan Wang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Type 3

Retrospective survey and phylogenetic analysis of porcine circovirus type 3 in Jiangsu province, China, 2008 to 2017

2018 ◽  
Vol 163 (9) ◽  
pp. 2531-2538 ◽  
Author(s):  
Dong Zhao ◽  
Xiaobo Wang ◽  
Qingqing Gao ◽  
Changchao Huan ◽  
Wanbin Wang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Porcine Circovirus Type ◽  
Jiangsu Province ◽  
Porcine Circovirus ◽  
Retrospective Survey ◽  
Type 3

Genetic diversity and prevalence of porcine circovirus type 3 (PCV3) and type 2 (PCV2) in the Midwest of the USA during 2016–2018

2020 ◽  
Vol 67 (3) ◽  
pp. 1284-1294 ◽  
Author(s):  
Yin Wang ◽  
Lance Noll ◽  
Nanyan Lu ◽  
Elizabeth Porter ◽  
Colin Stoy ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
The Usa ◽  
Type 3

Molecular detection and sequence analysis of porcine circovirus type 3 in sow sera from farms with prolonged histories of reproductive problems in Hunan, China

2018 ◽  
Vol 163 (10) ◽  
pp. 2841-2847 ◽  
Author(s):  
Yawen Zou ◽  
Nanxiangzi Zhang ◽  
Jiaxin Zhang ◽  
Sujiao Zhang ◽  
Yifan Jiang ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Molecular Detection ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Type 3

First report of wild boar susceptibility to Porcine circovirus type 3: High prevalence in the Colli Euganei Regional Park (Italy) in the absence of clinical signs

2018 ◽  
Vol 65 (4) ◽  
pp. 957-962 ◽  
Author(s):  
Giovanni Franzo ◽  
Claudia Maria Tucciarone ◽  
Michele Drigo ◽  
Mattia Cecchinato ◽  
Marco Martini ◽  
...  
Keyword(s):  
Wild Boar ◽  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
First Report ◽  
High Prevalence ◽  
Type 3

scholarly journals First detection of porcine circovirus type 3 in Japan

2018 ◽  
Vol 80 (9) ◽  
pp. 1468-1472 ◽  
Author(s):  
Shizuka HAYASHI ◽  
Yoshiyuki OHSHIMA ◽  
Yoshiaki FURUYA ◽  
Aki NAGAO ◽  
Kazuki OROKU ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Type 3
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