The presence and genetic characteristics of porcine circovirus 3 from pigs in Southern and Central provinces of Vietnam

2020 ◽  
pp. 26-31
Author(s):  
Giao N. P. Trinh
Keyword(s):  
Gene Segment ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Nucleotide Sequencing ◽  
Duplex Pcr ◽  
Serum Samples ◽  
Swine Industry ◽  
Genetic Characteristics ◽  
Swine Herds ◽  
Type 3

Porcine circovirus type 3 (PCV3) is an emerging circovirus species that has recently been reported in different countries around the world, suggesting a widespread circulation. This study was carried out in order to investigate the presence and further genetic characteristics of PCV3 from swine herds in Southern and Central provinces of Vietnam. A duplex PCR assay for rapid detection of PCV3 in pigs was established with a pair of specific primers designed between rep and cap gene segment to amplify full-length ORF2 and another set of primers binding to COX1gene serving as an internal amplification control (IAC). The resulting duplex PCR was used to examine PCV3 presence in 94tissue and serum samples. Subsequently, PCV3 was detected in 10 out of 94 cases (10.6%). The infection rate in sows (14.3%) was higher than that in grower pigs (7.7%). Regarding nucleotide sequence comparison, 10 ORF2 genes were selected for nucleotide sequencing and their alignment showed 97.2% - 99.5% homology. According to the phylogenetic analysis and sequence alignment of cap gene, all the sequences were clustered into group PCV3a,including 9 strains of sub-group PCV3a1 and only one strain of subgroup PCV3a2. These findings indicated that the PCV3a group is circulating in swine farms in Vietnam. This study provides better insights into epidemiology of this pathogen in the national swine industry.

scholarly journals Identification of porcine circovirus type 2 (PCV2), type 3 (PCV3) and porcine parvovirus (PPV) in swine by multiplex PCR test

2021 ◽  
Vol 20 (03) ◽  
pp. 11-17
Author(s):  
Phat X. Dinh
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Serum Samples ◽  
Single Target ◽  
Ns1 Gene ◽  
Type 3

This study aimed to simultaneously detect three important viruses reported to be involved in the reproductive problems of sows. A multiplex PCR (mPCR) test was developed to provide rapid diagnosis of porcine circovirus type 2 and 3 (PCV2, PCV3) and to illustrate parvovirus (PPV) prevalence in sow herds. Three pairs of specific primers were designed to target PCV2 Cap gene, PCV3 Cap gene and PPV NS1 gene, with predicted mPCR products of 702 bp, 267 bp and 380 bp, respectively. The detection limit of mPCR was 100 copies/reaction per target gene. The mPCR was run against a panel of 94 swine serum samples whose infection status had been pre-determined by commercial real-time PCR kits. Sequencing of mPCR products performed with clinical serum samples accurately confirmed the results. Overall, the results indicated that the mPCR functioned accurately and specifically and matched 100% with the single-target real-time PCRs. The mPCR was developed successfully and can be used in routine diagnosis of PCV2, PCV3 and PPV.

scholarly journals Molecular Investigation of Porcine Circovirus Type 3 Infection in Pigs in Namibia

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 585
Author(s):  
Umberto Molini ◽  
Giuseppe Marruchella ◽  
Frieda Matheus ◽  
Yvonne Maria Hemberger ◽  
Bernard Chiwome ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Swine Industry ◽  
Domestic Pig ◽  
Pig Production ◽  
Molecular Investigation ◽  
Low Levels ◽  
Type 3 ◽  
Production Facilities

Porcine circovirus type 3 (PCV-3) infection is widely distributed in domestic pig populations in America, Europe, and Asia. However, no data is currently available about its presence and distribution in Africa. This study investigated the presence of PCV-3 in pigs (n = 122) in Namibia, by means of biomolecular methods. The pig samples collected (n = 122) were representative of the swine industry in Namibia, covering the major pig production facilities in the country. All of the samples tested were negative for PCV-3, and this indicated that the virus was either not present in the country or was circulating at low levels. Further studies are needed to better understand the distribution, if any, of PCV-3 in Namibia.

scholarly journals Complete Genome Sequence of a Novel Porcine Circovirus Type 3 Strain, CH/GX/1776D/2017, Isolated from Guangxi, China

2018 ◽  
Vol 6 (17) ◽  
Author(s):  
Bingxia Lu ◽  
Yibin Qin ◽  
Ying He ◽  
Lei Liu ◽  
Qunpeng Duan ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Sequence Data ◽  
Southern China ◽  
The United States ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Molecular Characteristics ◽  
Guangxi Province ◽  
Swine Herds ◽  
Type 3

ABSTRACT Porcine circovirus type 3 (PCV3) was first described in 2016 in U.S. swine herds as a pathogenic agent for pigs. To date, PCV3 has been reported to be widely circulating in the United States, China, South Korea, Brazil, Italy, and Poland. Here, we report the genome sequence of a PCV3 strain (CH/GX/1776D/2017) isolated from Guangxi Province in southern China. The sequence data presented in this study will help us better understand the molecular characteristics and genetic diversity of PCV3 in China.

scholarly journals Complete Genome Sequence of a Novel Porcine Circovirus Type 3 Strain, PCV3/CN/Hubei-618/2016, Isolated from China

2017 ◽  
Vol 5 (15) ◽  
Author(s):  
Shengxian Fan ◽  
Xugang Ku ◽  
Fangzhou Chen ◽  
Ying Wang ◽  
Xuexiang Yu ◽  
...  
Keyword(s):  
United States ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
The United States ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Genetic Characteristics ◽  
Type 3

ABSTRACT In October 2016, porcine circovirus type 3 (PCV3) was identified as a pathogen agent for pigs in the United States. Here, we report the genome sequence of a Chinese PCV3 strain, PCV3/CN/Hubei-618/2016. This will help us better understand the epidemiology and genetic characteristics of PCV3.

scholarly journals Prevalence and Genetic Analysis of Porcine Circovirus 3 in China From 2019 to 2020

2021 ◽  
Vol 8 ◽  
Author(s):  
Meng Ge ◽  
Jie Ren ◽  
Yi-Lin Xie ◽  
Dun Zhao ◽  
Fang-Cheng Fan ◽  
...  
Keyword(s):  
Potential Effect ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Reading Frame ◽  
Humoral Immune ◽  
Antibody Positivity ◽  
Repeated Infections ◽  
Type 3

Porcine circovirus type 3 (PCV3), a virus belonging to the Circoviridae family, is considered to be associated with respiratory and neurological signs, cardiac and multisystemic inflammation, reproductive failure, and porcine dermatitis and nephropathy syndrome-like disease in pigs (Sus scrofa). In this study, epidemiological and serological investigations of PCV3 in clinically healthy pigs from different regions of China were performed. Overall, 42.87% (1,101/2,568) of pigs were positive for PCV3 Cap antibody via indirect enzyme-linked immunosorbent assay, with a higher prevalence of PCV3 in multiparous sows (62.22%, 881/1,416) and fattening pigs (28.96%, 159/549) than in suckling piglets (8.96%, 32/357) and nursery pigs (11.79%, 29/246). Of the 2,568 samples, 255 were further tested for PCV3 DNA using real-time polymerase chain reaction, and 63.14% of these were positive, with nearly half having <10 virus copies. The PCV3 DNA and antibody positivity rates were high in the pig serum samples; however, the virus titers and antibody levels were both low, indicating that the humoral immune response of PCV3-infected pigs was weak or lagging, and persistent or repeated infections could occur. Additionally, the complete genomes of 23 PCV3 strains were sequenced and analyzed, which showed nucleotide identities of 98.5~100.0%, 98.6~100.0%, and 99.2~100.0% in the complete genome, open reading frame (ORF)2, and ORF1 sequences, respectively, and amino acid identities of 96.7~100.0% and 99.3~100.0% in the capsid and replicase proteins, respectively. Phylogenetic analysis based on ORF2 nucleotide sequences indicated that the PCV3 strains obtained in the present study could be classified into three sub-clades, with most strains clustered into clade 3c, indicating that PCV3c is the dominant subtype in the regions of China investigated. In general, the present study revealed a high prevalence and high genetic divergence of PCV3 among Chinese pig herds, and indicated that the potential effect of PCV3 on the pig industry may be a concern.

scholarly journals Nucleolar Phosphoprotein NPM1 Interacts With Porcine Circovirus Type 3 Cap Protein and Facilitates Viral Replication

2021 ◽  
Vol 12 ◽  
Author(s):  
Jiangwei Song ◽  
Lei Hou ◽  
Dan Wang ◽  
Li Wei ◽  
Shanshan Zhu ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Mass Spectrometry Analysis ◽  
Cellular Proteins ◽  
Porcine Circovirus ◽  
Amino Acid Residues ◽  
Liquid Chromatography Mass Spectrometry ◽  
Swine Industry ◽  
Spectrometry Analysis ◽  
Infected Cells ◽  
Type 3

Porcine circovirus type 3 (PCV3) is a recently discovered virus with potentially significant implications on the global swine industry. PCV3 replication involves the entry of the viral capsid (Cap) protein with nucleolar localization signals into the nucleus. Using liquid chromatography-mass spectrometry analysis, nucleolar phosphoprotein NPM1 was identified as one of the cellular proteins bound to PCV3 Cap. Co-immunoprecipitation demonstrated that PCV3 Cap interacts directly with NPM1, where the region binding with NPM1 is mapped to amino acid residues 1–38 of Cap. Upon co-transfection, the expression of Cap protein promoted the redistribution of NPM1, which translocated from the nucleus to the cytoplasm and colocalized with Cap in cultured PK15 cells. NPM1 expression was upregulated and translocated from the nucleus to the cytoplasm in PCV3-infected cells, upon siRNA-mediated depletion, or upon treatment with NPM1 inhibitor in PK15 cells with impaired PCV3 replication, as evidenced by decreased levels of viral DNA synthesis and protein expression. By contrast, the replication of PCV3 was enhanced in stably NPM1-expressing cells via a lentivirus-delivered system. Taken together, these findings indicate that NPM1 interacts with PCV3 Cap and plays a crucial role in PCV3 replication.

scholarly journals Molecular Epidemiology of Porcine Circovirus Type 3 Infection in Swine Herds in China

2018 ◽  
Vol 33 (4) ◽  
pp. 373-377 ◽  
Author(s):  
Xiangdong Li ◽  
Chaolin Zhang ◽  
Mingming Qiao ◽  
Jiangsong Guo ◽  
Guangyuan Xing ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Swine Herds ◽  
Type 3

scholarly journals Identification of Porcine circovirus type (PCV2) and type 3 (PCV3), Porcine 2 parvovirus (PPV) in swine by multiplex PCR test

2020 ◽  
Author(s):  
Thoai Kim Tran ◽  
Trang Thi Thanh Nguyen ◽  
Hiep Lai Xuan Vu ◽  
Phat Xuan Dinh
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Porcine Circovirus Type ◽  
Rapid Diagnosis ◽  
Porcine Circovirus ◽  
Serum Samples ◽  
Ns1 Gene ◽  
Type 3 ◽  
Swine Serum

Abstract Background : Aiming to simultaneously detect three important viruses known to be involved in reproductive problems of sows, a multiplex PCR (mPCR) test was developed to provide rapid diagnosis of porcine circovirus type 2 and 3 (PCV2, PCV3) and to illustrate parvovirus (PPV) prevalence in sow herds. Methods : Three pairs of specific primers were designed to target PCV2 Cap gene, PCV3 Cap gene and PPV NS1 gene, with predicted mPCR products of 702 bp, 267 bp and 380 bp, respectively. Results : The detection limit of mPCR was 100 copies/ reaction per target gene. Sequencing of mPCR products performed with clinical serum samples accurately confirmed results. The mPCR was run against a panel of 94 swine serum samples whose infection status had been pre-determined by commercial real-time PCR kits. Overall, the mPCR results matched 100% with the real-time PCRs. Conclusions : The developed mPCR test functions successfully and can be used in routine rapid diagnosis of PCV2, PCV3 and PPV.

scholarly journals Molecular detection of porcine circovirus type 3 in Shanxi Province, China

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Weidong Yue ◽  
Yihui Liu ◽  
Xinrong Zhang ◽  
Haili Ma ◽  
Junping He
Keyword(s):  
Genomic Sequence ◽  
The United States ◽  
Porcine Circovirus Type ◽  
Shanxi Province ◽  
Porcine Circovirus ◽  
Swine Industry ◽  
Double Positive ◽  
Potential Threat ◽  
Tissue Samples ◽  
Type 3

AbstractPorcine circovirus type 3 (PCV3), which was first detected in the United States of America in 2015, is a potential threat to the swine industry. However, the prevalence of PCV3 in Shanxi Province, China, is unclear. In this research, the prevalence and genetic diversity of PCV3 were investigated in above area. Lung tissue samples (n = 491) from 19 pig slaughterhouses across 11 cities throughout Shanxi Province were analyzed for PCV3 infection by PCR in 2019. The results showed that PCV3 positive rates in slaughterhouses and individuals were 100% (19/19) and 86.76% (426/491), respectively. PCV2 and PCV3 double-positive rates in slaughterhouses and individuals were 100% (19/19) and 59.27% (291/491), respectively. PCR positive samples were further sequenced and 8 PCV3 isolates were identified. The nucleotide homology of these isolates with other PCV3 isolates in NCBI database was 97.45–99.90%. A phylogenetic analysis, based on the complete genomic sequence and ORF2, divided these PCV3 strains into 2 major groups. Based on A24/V and R27/K amino acid mutations of capsid protein, the 8 identified PCV3 strains were separated to 2 clades. This was the first detailed investigation into the epidemiology of PCV3 in Shanxi Province. Our findings enabled us to assess the possibility of widespread transmission from this region. Thus, current findings establish a basis for further studies of genetic variations in PCV3 strains circulating in China.

scholarly journals A Molecular Survey and Phylogenetic Analysis of Porcine Circovirus Type 3 Using Oral Fluid, Faeces and Serum

2020 ◽  
Author(s):  
Jan Plut ◽  
Urska Jamnikar-Ciglenecki ◽  
Irena Golinar-Oven ◽  
Tanja Knific ◽  
Marina Stukelj
Keyword(s):  
Genome Sequencing ◽  
Oral Fluid ◽  
Statistical Significance ◽  
Age Groups ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Serum Samples ◽  
Type 3

Abstract Background: Porcine circovirus type 3 is the most recently discovered porcine circovirus, and an emerging pathogen. In this study the status of its presence on some Slovenian farms is reported. The effectiveness of the vaccine against porcine circovirus type 2 was assessed against porcine circovirus type 3.Group samples of oral fluid, faeces and individual serum samples were taken from six different pig categories and tested for presence of viral DNA, using both real time and conventional PCR. Positive samples were subjected to direct Sanger sequencing. Nucleotide sequences were analyzed and compared to GenBank PCV3 sequences.Results: Positive samples were sent for genome sequencing, which confirmed the presence of virus in all different pig categories on five farms. A high to moderate correlation of strong statistical significance was found between individual serum samples, oral fluid and faeces. Slovenian PCV3 was found to be distributed in a way similar to that of other countries. Slovenian PCV3 nt sequences are highly related, sharing more than 99.5 % nt identity. On one farm a commercially available vaccine against porcine circovirus type 2 was used on 3-week-old pigs. It did not affect the presence of porcine circovirus type 3 in oral fluid or sera of any of the seven age groups of pigs, each with two control groups.Conclusions: The results constitute the first discovery of the virus in Slovenia. Genome sequencing has revealed a high degree of similarity between Slovenian and GenBank isolates.

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