First report of wild boar susceptibility to Porcine circovirus type 3: High prevalence in the Colli Euganei Regional Park (Italy) in the absence of clinical signs

2018 ◽  
Vol 65 (4) ◽  
pp. 957-962 ◽  
Author(s):  
Giovanni Franzo ◽  
Claudia Maria Tucciarone ◽  
Michele Drigo ◽  
Mattia Cecchinato ◽  
Marco Martini ◽  
...  
Keyword(s):  
Wild Boar ◽  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
First Report ◽  
High Prevalence ◽  
Type 3

scholarly journals Porcine Circovirus Type 3 in Pig Farms Experiencing Diarrhea in Jiangxi, China: Prevalence, Genome Sequence and Pathogenicity

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2324
Author(s):  
Fanfan Zhang ◽  
Weifeng Yuan ◽  
Zhiquan Li ◽  
Yuhan Zhang ◽  
Xiuxiu Zeng ◽  
...  
Keyword(s):  
Complete Genome ◽  
Amino Acid Level ◽  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Weaned Piglets ◽  
Gross Lesions ◽  
Genome Level ◽  
Genome Analyses ◽  
Type 3

Porcine circovirus 3 (PCV3) infections have been reported in different clinical presentations. However, the prevalence and pathogenicity of PCV3 associated with diarrhea in piglets have been limited. Herein, we present an investigation and genome analyses of PCV3 in piglets experiencing diarrhea, and observed clinical signs, gross lesions, and histological changes in pigs negative for all known pathogens associated with diarrhea but positive for PCV3 alone. Among the feces (n = 141) tested, 16.31% (23/141) were positive for PCV3. Of which, 27.28% (15/55) and 14.29% (5/35) were present in diarrheal samples from suckling and weaned piglets, respectively. Moderate to severe atrophic villi was confined in duodenum, jejunum, and ileum, and significantly decreased average heights of villi, and the depths of crypt were observed in PCV3-infected piglets. The complete genome of a representative strain of PCV3, designated as JX/CH/2018, was determined. Multialignment analysis indicated that JX/CH/2018 had 97.7–99.7% nucleotide identity at the complete genome level, and 97.2–100% at the amino acid level of the capsid protein when compared with reference PCV3 strains. Phylogenetic analysis showed that the PCV3 strain identified in this study belonged to PCV3a lineage. The present study demonstrated that PCV3 is a common virus in diarrheal suckling and weaned piglets.

scholarly journals Induction of Porcine Dermatitis and Nephropathy Syndrome in Piglets by Infection with Porcine Circovirus Type 3

2018 ◽  
Vol 93 (4) ◽  
Author(s):  
Haijun Jiang ◽  
Dan Wang ◽  
Jing Wang ◽  
Shanshan Zhu ◽  
Ruiping She ◽  
...  
Keyword(s):  
Specific Antigen ◽  
Clinical Signs ◽  
Keyhole Limpet Hemocyanin ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Significant Information ◽  
Pathological Lesions ◽  
Cytokines And Chemokines ◽  
Specific Pathogen ◽  
Type 3

ABSTRACT Porcine circovirus type 3 (PCV3) is an emerging porcine circovirus that has been associated with porcine dermatitis and nephropathy syndrome (PDNS)-like clinical signs, reproductive failure, cardiac pathologies, and multisystemic inflammation in piglets and sows. Many aspects of PCV3 infection biology and pathogenesis, however, remain unknown. Here, we used a PCV3 virus stock from the rescue of an infectious PCV3 DNA clone to intranasally inoculate 4- and 8-week-old specific-pathogen-free piglets for evaluation of PCV3 pathogenesis. For 4-week-old piglets, typical clinical signs resembling those of PDNS-like disease were observed when piglets were inoculated with PCV3 alone or PCV3 combined with immunostimulation by keyhole limpet hemocyanin, with a mortality of 40% (2/5) for both types of inoculated piglets during a 28-day observation period postinoculation. Both types of inoculated piglets showed similar progressive increases in viral loads in the sera and had seroconverted to PCV3 capsid antibody after inoculation. Pathological lesions and PCV3-specific antigen were detected in various tissues and organs, including the lung, heart, kidney, lymph nodes, spleen, liver, and small intestine, in both types of inoculated piglets. The levels of proinflammatory cytokines and chemokines, including interleukin 1 beta (IL-1β), IL-6, IL-23α, gamma interferon (IFN-γ), tumor necrosis factor alpha (TNF-α), and chemokine ligand 5 (CCL5), were significantly upregulated in both groups of inoculated piglets. Eight-week-old piglets also exhibited a similar PDNS-like disease but without death after PCV3 inoculation, as evidenced by pathological lesions and PCV3 antigen in various tissues and organs. These results show for the first time successful reproduction of PDNS-like disease by PCV3 infection and further provide significant information regarding the pathogenesis of PCV3 in piglets. IMPORTANCE Porcine circovirus type 3 (PCV3), an emerging porcine circovirus, is considered the cause of porcine dermatitis and nephropathy syndrome (PDNS)-like clinical signs and other systemic diseases in piglets and sows. To evaluate the pathogenesis of PCV3 infection in vivo, we used a PCV3 virus stock from the rescue of an infectious PCV3 DNA clone to intranasally inoculate 4- and 8-week-old specific-pathogen-free piglets and demonstrated successful reproduction of PDNS-like disease in animals that were inoculated with PCV3 alone or PCV3 combined with immunostimulation by keyhole limpet hemocyanin. Both 4- and 8-week-old PCV3-inoculated piglets showed similar increases in viral loads in the sera and had seroconverted to PCV3 capsid antibody. Pathological lesions and PCV3-specific antigen were detected in various tissues and organs, while numerous proinflammatory cytokines and chemokines in the sera were significantly upregulated after PCV3 inoculation. These results will provide significant information regarding the pathogenesis of PCV3 in piglets.

scholarly journals High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Bing Yan Liu ◽  
Bin Gao ◽  
Meng Zhi Liu ◽  
Ting Ting Zhang ◽  
Bao Shan Liu ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Vaccine Candidate ◽  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Reproductive Failure ◽  
Porcine Circovirus ◽  
Effective Vaccine ◽  
E Coli ◽  
Efficient Expression ◽  
Type 3

AbstractPorcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitive arginine (ΔCAP) and their codon-optimized counterparts in E. coli. These results showed that lots of repeated arginine could severely lower the expression of PCV3 capsid protein in E. coli. At the same time, the recombined truncated PCV3 capsid protein forms typic virions. The efficient expression of capsid protein is expected to serve the development of PCV3 vaccines and other studies of PCV3 capsid protein.

scholarly journals Complete Genome Sequences of Two Type 3 Porcine Circoviruses, WB17KW and WB20GG, Isolated from Korean Wild Boar

2021 ◽  
Vol 10 (6) ◽  
Author(s):  
Sok Song ◽  
Kyu-Nam Park ◽  
SeEun Choe ◽  
Ra Mi Cha ◽  
Jihye Shin ◽  
...  
Keyword(s):  
South Korea ◽  
Wild Boar ◽  
Complete Genome ◽  
Genetic Relatedness ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Genome Sequences ◽  
Domestic Pigs ◽  
Porcine Circoviruses ◽  
Type 3

ABSTRACT Porcine circovirus type 3 (PCV3) in domestic pigs was first reported in South Korea in 2017. Here, we report the first complete genome sequences of two PCV3 strains isolated from Korean wild boar, which enhance our understanding about the genetic relatedness of PCV3 in domestic pigs and wild boar.

scholarly journals Porcine Gammaherpesviruses in Italian Commercial Swine Population: Frequent but Harmless

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 47
Author(s):  
Giovanni Franzo ◽  
Michele Drigo ◽  
Matteo Legnardi ◽  
Laura Grassi ◽  
Maria Luisa Menandro ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Clinical Signs ◽  
Northern Italy ◽  
Porcine Circovirus ◽  
Swine Industry ◽  
Positive Interaction ◽  
High Prevalence ◽  
Swine Population ◽  
Clinical Conditions

Differently from alpha- and betaherpesviruses affecting swine, interest in the recently discovered Suid gammaherpesvirus 3, Suid gammaherpesvirus 4, and Suid gammaherpesvirus 5, also known as porcine lymphotropic herpesviruses (PLHV-1, PLHV-2, and PLHV-3), has largely focused on their role as potential zoonotic agents in cases of xenotransplantation. However, their role as primary pathogens of swine or as co-factors for other lymphotropic infections has essentially been neglected. The present study aims at filling this gap, evaluating the association between PLHVs infection and different clinical conditions and/or porcine circovirus (PCV) co-infection. One hundred seventy-six samples were obtained from different animals located in a high-density pig area of Northern Italy in the period 2017–2020. The presence of PLHVs and PCVs was tested and quantified by specific real-time PCR: PLHVs were widespread among pigs (PLHV-1, PLHV-2, and PLHV-3 prevalence was 28.97%, 10.79%, and 4.54%, respectively) and detected in all considered tissues and clinical conditions. Frequent co-infections were also observed among PLHVs and with PCVs, although a significant association was not detected with the exception of a positive interaction between PLHV-1 and PLHV-3, and a negative one between PLHV-2 and PCV-2. Significantly, no association between PLHVs, alone or in co-infection, emerged with any of the considered clinical signs, their frequency being comparable between healthy and diseased animals. Based on these pieces of evidence and despite their high prevalence, PLHVs’ relevance for the swine industry appears negligible, either as primary pathogens or as predisposing factors for circovirus-induced diseases.

scholarly journals Description of a closed pyometra on a wild boar (Sus scrofa, Linnaeus 1758)

2021 ◽  
Vol 53 (3) ◽  
Author(s):  
Andreia Garcês ◽  
Vanessa Soeira ◽  
Sara Lóio ◽  
Isabel Pires
Keyword(s):  
Wild Boar ◽  
Body Condition ◽  
Sus Scrofa ◽  
Unusual Case ◽  
Clinical Signs ◽  
Advanced Age ◽  
Uterine Lumen ◽  
First Report ◽  
Geriatric Conditions ◽  
Large Uterus

Pyometra is a pus accumulation in the uterine lumen. This paper reports an unusual case of pyometra in a female Sus scrofa of Parque Biologico de Gaia (Avintes, Portugal). The animal was of advanced age, and the clinical signs (e.g., anorexia, lethargy) were originally associated with geriatric conditions. The animal presented a large uterus, which likely further contributed to the locomotion difficulties and poor body condition. To the extent of our knowledge, this is the first report of a close pyometra in wild boar (Sus scrofa, Linnaeus, 1758). However, this diagnosis must be taken into account during uterine evaluation and special attention must be given to its inspection in meat animals.

scholarly journals Diagnosis of post-weaning multisystemic wasting syndrome in pigs in Brazil caused by porcine circovirus type 2

2003 ◽  
Vol 55 (5) ◽  
pp. 522-527 ◽  
Author(s):  
J.R. Ciacci-Zanella ◽  
N. Morés
Keyword(s):  
Clinical Signs ◽  
Rflp Analysis ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Nested Polymerase Chain Reaction ◽  
Wasting Syndrome ◽  
Thymus Atrophy ◽  
Granulomatous Lymphadenitis

This report describes the first preliminary characterization of porcine circovirus type 2 (PCV2) isolates from pigs affected with post-weaning multisystemic wasting syndrome (PMWS) in Brazil. Diseased pigs were examined at necropsy and by histopathology. Macroscopic and microscopic analyses revealed lesions reported to be typical of PMWS, which included, respectively, emaciation, enlargement of lymph nodes, thymus atrophy and interstitial pneumonia, and granulomatous lymphadenitis with syncytial cells, among others. Using nested polymerase chain reaction (PCR) or imunoperoxidase it was possible to detected DNA or antigen of PCV2, respectively. The PCR' s amplified fragment could be differentiated from PCV1 and PCV2 from one another by restriction fragment length polymorphism (RFLP) analysis. PCV2 DNA was detected in 70% (14/20) of samples of pigs with clinical signs and lesions associated with PMWS. This study shows that PCV2 is associated with lesions and symptoms indicative of PMWS in pigs. It is also shown that the Brazilian PCV2 isolates may have variation in their genome.

Production of a monoclonal antibody against Porcine circovirus type 3 cap protein

2018 ◽  
Vol 261 ◽  
pp. 10-13 ◽  
Author(s):  
Xiangdong Li ◽  
Yilin Bai ◽  
Haiyang Zhang ◽  
Dingding Zheng ◽  
Tongyan Wang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Type 3

Retrospective survey and phylogenetic analysis of porcine circovirus type 3 in Jiangsu province, China, 2008 to 2017

2018 ◽  
Vol 163 (9) ◽  
pp. 2531-2538 ◽  
Author(s):  
Dong Zhao ◽  
Xiaobo Wang ◽  
Qingqing Gao ◽  
Changchao Huan ◽  
Wanbin Wang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Porcine Circovirus Type ◽  
Jiangsu Province ◽  
Porcine Circovirus ◽  
Retrospective Survey ◽  
Type 3
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